YMR153W / NUP53

FG-nucleoporin component of central core of nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport; involved in regulation of transcription and mitosis; induces membrane tubulation, which may contribute to nuclear pore assembly; NUP53 has a paralog, ASM4, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YMR153W / NUP53

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:3.88|P-value:2.59E-5 ID:SGTC_373|Compound:0844-0104|FD-Score:3.36|P-value:2.22E-4 ID:SGTC_403|Compound:arphamenine a|FD-Score:3.02|P-value:7.97E-4 ID:SGTC_421|Compound:streptovitacin|FD-Score:3.04|P-value:7.38E-4 ID:SGTC_446|Compound:go6976|FD-Score:3.00|P-value:8.56E-4 ID:SGTC_477|Compound:mead ethanolamide|FD-Score:4.42|P-value:2.04E-6 ID:SGTC_493|Compound:zm226600|FD-Score:3.77|P-value:4.22E-5 ID:SGTC_511|Compound:blebbistatin|FD-Score:-3.21|P-value:4.25E-4 ID:SGTC_573|Compound:3702-0553|FD-Score:3.29|P-value:2.93E-4 ID:SGTC_754|Compound:3474-0146|FD-Score:6.33|P-value:2.09E-11 ID:SGTC_757|Compound:1066-0008|FD-Score:3.19|P-value:4.38E-4 ID:SGTC_881|Compound:0833-0569|FD-Score:4.12|P-value:8.41E-6 ID:SGTC_889|Compound:0919-1524|FD-Score:3.07|P-value:6.69E-4 ID:SGTC_965|Compound:1291-5240|FD-Score:4.46|P-value:1.67E-6 ID:SGTC_1232|Compound:0279-0259|FD-Score:3.31|P-value:2.70E-4 ID:SGTC_1272|Compound:0839-0021|FD-Score:3.38|P-value:2.10E-4 ID:SGTC_1334|Compound:1486-0610|FD-Score:3.47|P-value:1.48E-4 ID:SGTC_1358|Compound:1509-0022|FD-Score:3.71|P-value:5.38E-5 ID:SGTC_1486|Compound:3970-0793|FD-Score:3.02|P-value:8.20E-4 ID:SGTC_1617|Compound:st002906|FD-Score:3.23|P-value:3.75E-4 ID:SGTC_1631|Compound:benzylvanillin|FD-Score:3.18|P-value:4.48E-4 ID:SGTC_1663|Compound:st014140|FD-Score:5.08|P-value:5.85E-8 ID:SGTC_1664|Compound:st014217|FD-Score:3.02|P-value:8.00E-4 ID:SGTC_1799|Compound:st045822|FD-Score:4.46|P-value:1.68E-6 ID:SGTC_1967|Compound:st070177|FD-Score:3.48|P-value:1.43E-4 ID:SGTC_1983|Compound:st071813|FD-Score:4.18|P-value:6.53E-6 ID:SGTC_2006|Compound:4011487|FD-Score:3.12|P-value:5.71E-4 ID:SGTC_2038|Compound:4100038|FD-Score:3.58|P-value:9.31E-5 ID:SGTC_2259|Compound:7964009|FD-Score:5.94|P-value:2.92E-10 ID:SGTC_2282|Compound:7944268|FD-Score:5.20|P-value:2.93E-8 ID:SGTC_2329|Compound:6138490|FD-Score:3.25|P-value:3.51E-4 ID:SGTC_2377|Compound:9073351|FD-Score:3.52|P-value:1.18E-4 ID:SGTC_2412|Compound:st077135|FD-Score:-3.66|P-value:7.37E-5 ID:SGTC_2517|Compound:totarol|FD-Score:3.49|P-value:1.36E-4 ID:SGTC_2526|Compound:patulin|FD-Score:4.58|P-value:9.01E-7 ID:SGTC_2582|Compound:agaric acid|FD-Score:3.99|P-value:1.60E-5 ID:SGTC_2661|Compound:dactinomycin|FD-Score:-3.27|P-value:3.38E-4 ID:SGTC_2833|Compound:9001315|FD-Score:3.20|P-value:4.22E-4 ID:SGTC_2865|Compound:9030313|FD-Score:3.28|P-value:3.13E-4 ID:SGTC_2893|Compound:9048488|FD-Score:3.54|P-value:1.10E-4 ID:SGTC_2932|Compound:9016711|FD-Score:-4.82|P-value:2.75E-7 ID:SGTC_2937|Compound:9044753|FD-Score:-3.18|P-value:4.86E-4 ID:SGTC_2961|Compound:9083290|FD-Score:3.63|P-value:7.46E-5 ID:SGTC_3158|Compound:9100007|FD-Score:3.78|P-value:4.04E-5 ID:SGTC_3207|Compound:9114163|FD-Score:3.36|P-value:2.28E-4 ID:SGTC_3210|Compound:9128157|FD-Score:-3.31|P-value:2.99E-4 ID:SGTC_3323|Compound:9138564|FD-Score:4.13|P-value:8.20E-6 ID:SGTC_3325|Compound:9138760|FD-Score:5.89|P-value:4.20E-10 ID:SGTC_3333|Compound:9141820|FD-Score:3.50|P-value:1.28E-4 ID:SGTC_3334|Compound:9142812|FD-Score:-4.06|P-value:1.23E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:3.88|P-value:2.59E-5 ID:SGTC_373|Compound:0844-0104|FD-Score:3.36|P-value:2.22E-4 ID:SGTC_403|Compound:arphamenine a|FD-Score:3.02|P-value:7.97E-4 ID:SGTC_421|Compound:streptovitacin|FD-Score:3.04|P-value:7.38E-4 ID:SGTC_446|Compound:go6976|FD-Score:3.00|P-value:8.56E-4 ID:SGTC_477|Compound:mead ethanolamide|FD-Score:4.42|P-value:2.04E-6 ID:SGTC_493|Compound:zm226600|FD-Score:3.77|P-value:4.22E-5 ID:SGTC_511|Compound:blebbistatin|FD-Score:-3.21|P-value:4.25E-4 ID:SGTC_573|Compound:3702-0553|FD-Score:3.29|P-value:2.93E-4 ID:SGTC_754|Compound:3474-0146|FD-Score:6.33|P-value:2.09E-11 ID:SGTC_757|Compound:1066-0008|FD-Score:3.19|P-value:4.38E-4 ID:SGTC_881|Compound:0833-0569|FD-Score:4.12|P-value:8.41E-6 ID:SGTC_889|Compound:0919-1524|FD-Score:3.07|P-value:6.69E-4 ID:SGTC_965|Compound:1291-5240|FD-Score:4.46|P-value:1.67E-6 ID:SGTC_1232|Compound:0279-0259|FD-Score:3.31|P-value:2.70E-4 ID:SGTC_1272|Compound:0839-0021|FD-Score:3.38|P-value:2.10E-4 ID:SGTC_1334|Compound:1486-0610|FD-Score:3.47|P-value:1.48E-4 ID:SGTC_1358|Compound:1509-0022|FD-Score:3.71|P-value:5.38E-5 ID:SGTC_1486|Compound:3970-0793|FD-Score:3.02|P-value:8.20E-4 ID:SGTC_1617|Compound:st002906|FD-Score:3.23|P-value:3.75E-4 ID:SGTC_1631|Compound:benzylvanillin|FD-Score:3.18|P-value:4.48E-4 ID:SGTC_1663|Compound:st014140|FD-Score:5.08|P-value:5.85E-8 ID:SGTC_1664|Compound:st014217|FD-Score:3.02|P-value:8.00E-4 ID:SGTC_1799|Compound:st045822|FD-Score:4.46|P-value:1.68E-6 ID:SGTC_1967|Compound:st070177|FD-Score:3.48|P-value:1.43E-4 ID:SGTC_1983|Compound:st071813|FD-Score:4.18|P-value:6.53E-6 ID:SGTC_2006|Compound:4011487|FD-Score:3.12|P-value:5.71E-4 ID:SGTC_2038|Compound:4100038|FD-Score:3.58|P-value:9.31E-5 ID:SGTC_2259|Compound:7964009|FD-Score:5.94|P-value:2.92E-10 ID:SGTC_2282|Compound:7944268|FD-Score:5.20|P-value:2.93E-8 ID:SGTC_2329|Compound:6138490|FD-Score:3.25|P-value:3.51E-4 ID:SGTC_2377|Compound:9073351|FD-Score:3.52|P-value:1.18E-4 ID:SGTC_2412|Compound:st077135|FD-Score:-3.66|P-value:7.37E-5 ID:SGTC_2517|Compound:totarol|FD-Score:3.49|P-value:1.36E-4 ID:SGTC_2526|Compound:patulin|FD-Score:4.58|P-value:9.01E-7 ID:SGTC_2582|Compound:agaric acid|FD-Score:3.99|P-value:1.60E-5 ID:SGTC_2661|Compound:dactinomycin|FD-Score:-3.27|P-value:3.38E-4 ID:SGTC_2833|Compound:9001315|FD-Score:3.20|P-value:4.22E-4 ID:SGTC_2865|Compound:9030313|FD-Score:3.28|P-value:3.13E-4 ID:SGTC_2893|Compound:9048488|FD-Score:3.54|P-value:1.10E-4 ID:SGTC_2932|Compound:9016711|FD-Score:-4.82|P-value:2.75E-7 ID:SGTC_2937|Compound:9044753|FD-Score:-3.18|P-value:4.86E-4 ID:SGTC_2961|Compound:9083290|FD-Score:3.63|P-value:7.46E-5 ID:SGTC_3158|Compound:9100007|FD-Score:3.78|P-value:4.04E-5 ID:SGTC_3207|Compound:9114163|FD-Score:3.36|P-value:2.28E-4 ID:SGTC_3210|Compound:9128157|FD-Score:-3.31|P-value:2.99E-4 ID:SGTC_3323|Compound:9138564|FD-Score:4.13|P-value:8.20E-6 ID:SGTC_3325|Compound:9138760|FD-Score:5.89|P-value:4.20E-10 ID:SGTC_3333|Compound:9141820|FD-Score:3.50|P-value:1.28E-4 ID:SGTC_3334|Compound:9142812|FD-Score:-4.06|P-value:1.23E-5

Top fitness defect scores for YMR153W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_754 3474-0146 6.33 171.0 µM 2.09E-11 copper-dependent oxidative stress 4 1.23E-10
2 SGTC_2259 7964009 5.94 200.0 µM 2.92E-10 superoxide 14 1.41E-9
3 SGTC_3325 9138760 5.89 55.2 µM 4.20E-10 Golgi 8 1.97E-9
4 SGTC_2282 7944268 5.20 200.0 µM 2.93E-8 RPP1 & pyrimidine depletion 10 9.92E-8
5 SGTC_1663 st014140 5.08 55.4 µM 5.85E-8 60S ribosome export 9 1.88E-7
6 SGTC_2526 patulin 4.58 37.6 µM 9.01E-7 60 2.35E-6
7 SGTC_965 1291-5240 4.46 308.0 µM 1.67E-6 20 4.17E-6
8 SGTC_1799 st045822 4.46 46.1 µM 1.68E-6 7 4.19E-6
9 SGTC_477 mead ethanolamide 4.42 10.0 µM 2.04E-6 2 5.01E-6
10 SGTC_1983 st071813 4.18 46.9 µM 6.53E-6 RPP1 & pyrimidine depletion 11 1.47E-5
11 SGTC_3323 9138564 4.13 53.9 µM 8.20E-6 16 1.81E-5
12 SGTC_881 0833-0569 4.12 170.0 µM 8.41E-6 23 1.85E-5
13 SGTC_2582 agaric acid 3.99 100.0 µM 1.60E-5 redox potentiating 314 3.36E-5
14 SGTC_58 0957-0393 3.88 9.0 µM 2.59E-5 cell wall 381 5.24E-5
15 SGTC_3158 9100007 3.78 49.5 µM 4.04E-5 23 7.91E-5
16 SGTC_493 zm226600 3.77 134.0 µM 4.22E-5 mitochondrial processes 19 8.23E-5
17 SGTC_1358 1509-0022 3.71 2.5 µM 5.38E-5 15 1.03E-4
18 SGTC_2961 9083290 3.63 32.5 µM 7.46E-5 27 1.39E-4
19 SGTC_2038 4100038 3.58 31.0 µM 9.31E-5 plasma membrane duress 65 1.71E-4
20 SGTC_2893 9048488 3.54 26.0 µM 1.10E-4 fatty acid desaturase (OLE1) 30 2.01E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.143 6.66E-17 YML103C NUP188 hom Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC organization and nucleocytoplasmic transport; homologous to human NUP188
0.143 8.09E-17 YNL293W MSB3 hom Rab GTPase-activating protein; regulates endocytosis via activation of Vps21p and Ypt7p; also acts on Ypt52p and Sec4p; also required for proper actin organization; localizes to the plasma membrane and sites of polarized growth, and a portion localizes to endosomes and vacuoles; similar to Msb4p; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.117 1.03E-11 YIL160C POT1 hom 3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids
0.114 3.78E-11 YDL162C_d YDL162C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps ENT1/YDL161W, a verified gene involved in endocytosis and actin cortical patch assembly
0.113 4.88E-11 YOR231W MKK1 hom MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
0.112 7.75E-11 YNL241C ZWF1 hom Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidatve stress; homolog of the human G6PD which is deficient in patients with hemolytic anemia; protein abundance increases in response to DNA replication stress
0.112 8.90E-11 YIL123W SIM1 hom Protein of the SUN family (Sim1p, Uth1p, Nca3p, Sun4p) that may participate in DNA replication, promoter contains SCB regulation box at -300 bp indicating that expression may be cell cycle-regulated
0.111 1.02E-10 YLL039C UBI4 hom Ubiquitin; becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response; encoded as a polyubiquitin precursor comprised of 5 head-to-tail repeats; protein abundance increases in response to DNA replication stress
0.108 3.49E-10 YNL215W IES2 hom Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress
0.107 5.88E-10 YDR537C_d YDR537C_d hom Dubious open reading frame unlikely to encode a protein, almost completely overlaps verified ORF PAD1/YDR538W
0.105 8.96E-10 YNL027W CRZ1 hom Transcription factor, activates transcription of stress response genes; nuclear localization is positively regulated by calcineurin-mediated dephosphorylation; rapidly localizes to the nucleus under blue light stress
0.104 1.40E-9 YEL007W MIT1 hom Transcriptional regulator of pseudohyphal growth; protein with sequence similarity to S. pombe gti1+ (gluconate transport inducer 1) and C. albicans Wor1
0.104 1.72E-9 YIL111W COX5B hom Subunit Vb of cytochrome c oxidase; cytochrome c oxidase is the terminal member of the mitochondrial inner membrane electron transport chain; Cox5Bp is predominantly expressed during anaerobic growth while its isoform Va (Cox5Ap) is expressed during aerobic growth; COX5B has a paralog, COX5A, that arose from the whole genome duplication
0.104 1.76E-9 YMR111C YMR111C hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR111C is not an essential gene; forms nuclear foci upon DNA replication stress
0.104 1.81E-9 YLR206W ENT2 hom Epsin-like protein required for endocytosis and actin patch assembly and functionally redundant with Ent1p; contains clathrin-binding motif at C-terminus