YMR162C / DNF3

Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; localizes to the trans-Golgi network; likely involved in protein transport; type 4 P-type ATPase

Zygosity: Homozygous strain
fixedexpanded
Profile for YMR162C / DNF3

Click on Significant Values for Screen Details ID:SGTC_47|Compound:0149-0135|FD-Score:-3.27|P-value:2.96E-4 ID:SGTC_53|Compound:0973-0023|FD-Score:2.92|P-value:9.82E-4 ID:SGTC_72|Compound:0744-0168|FD-Score:3.39|P-value:1.60E-4 ID:SGTC_105|Compound:0111-0055|FD-Score:-3.05|P-value:6.87E-4 ID:SGTC_475|Compound:α-linolenic acid|FD-Score:3.29|P-value:2.41E-4 ID:SGTC_527|Compound:4476-4215|FD-Score:3.07|P-value:5.58E-4 ID:SGTC_621|Compound:3584-0052|FD-Score:4.05|P-value:8.81E-6 ID:SGTC_822|Compound:1487-0950|FD-Score:4.83|P-value:1.60E-7 ID:SGTC_823|Compound:0417-1665|FD-Score:-3.90|P-value:2.03E-5 ID:SGTC_828|Compound:2074-0507|FD-Score:3.95|P-value:1.39E-5 ID:SGTC_890|Compound:0929-0006|FD-Score:3.00|P-value:7.40E-4 ID:SGTC_997|Compound:3996-0078|FD-Score:3.40|P-value:1.53E-4 ID:SGTC_1221|Compound:2958-7315|FD-Score:3.66|P-value:5.11E-5 ID:SGTC_1309|Compound:pyrazolanthrone|FD-Score:2.93|P-value:9.32E-4 ID:SGTC_1476|Compound:4112-3294|FD-Score:-3.05|P-value:6.87E-4 ID:SGTC_1491|Compound:4138-0063|FD-Score:2.95|P-value:8.74E-4 ID:SGTC_1747|Compound:st037496|FD-Score:-4.67|P-value:4.56E-7 ID:SGTC_1819|Compound:st053448|FD-Score:-3.63|P-value:6.93E-5 ID:SGTC_1824|Compound:st053723|FD-Score:3.74|P-value:3.65E-5 ID:SGTC_2078|Compound:5212016|FD-Score:3.78|P-value:3.12E-5 ID:SGTC_2263|Compound:7948595|FD-Score:2.96|P-value:8.32E-4 ID:SGTC_2276|Compound:7947513|FD-Score:-2.99|P-value:8.42E-4 ID:SGTC_2309|Compound:7664088|FD-Score:3.12|P-value:4.58E-4 ID:SGTC_2380|Compound:9035439|FD-Score:3.73|P-value:3.76E-5 ID:SGTC_2412|Compound:st077135|FD-Score:3.18|P-value:3.68E-4 ID:SGTC_2461|Compound:5755302|FD-Score:3.18|P-value:3.69E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:3.27|P-value:2.60E-4 ID:SGTC_2616|Compound:abietic acid|FD-Score:3.19|P-value:3.60E-4 ID:SGTC_2629|Compound:bisabolol|FD-Score:3.36|P-value:1.85E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-3.30|P-value:2.66E-4 ID:SGTC_3328|Compound:9140624|FD-Score:5.49|P-value:3.03E-9 ID:SGTC_3330|Compound:9141424|FD-Score:3.95|P-value:1.40E-5 ID:SGTC_3332|Compound:9141069|FD-Score:5.24|P-value:1.47E-8 ID:SGTC_3356|Compound:9151811|FD-Score:-3.75|P-value:3.96E-5 ID:SGTC_47|Compound:0149-0135|FD-Score:-3.27|P-value:2.96E-4 ID:SGTC_53|Compound:0973-0023|FD-Score:2.92|P-value:9.82E-4 ID:SGTC_72|Compound:0744-0168|FD-Score:3.39|P-value:1.60E-4 ID:SGTC_105|Compound:0111-0055|FD-Score:-3.05|P-value:6.87E-4 ID:SGTC_475|Compound:α-linolenic acid|FD-Score:3.29|P-value:2.41E-4 ID:SGTC_527|Compound:4476-4215|FD-Score:3.07|P-value:5.58E-4 ID:SGTC_621|Compound:3584-0052|FD-Score:4.05|P-value:8.81E-6 ID:SGTC_822|Compound:1487-0950|FD-Score:4.83|P-value:1.60E-7 ID:SGTC_823|Compound:0417-1665|FD-Score:-3.90|P-value:2.03E-5 ID:SGTC_828|Compound:2074-0507|FD-Score:3.95|P-value:1.39E-5 ID:SGTC_890|Compound:0929-0006|FD-Score:3.00|P-value:7.40E-4 ID:SGTC_997|Compound:3996-0078|FD-Score:3.40|P-value:1.53E-4 ID:SGTC_1221|Compound:2958-7315|FD-Score:3.66|P-value:5.11E-5 ID:SGTC_1309|Compound:pyrazolanthrone|FD-Score:2.93|P-value:9.32E-4 ID:SGTC_1476|Compound:4112-3294|FD-Score:-3.05|P-value:6.87E-4 ID:SGTC_1491|Compound:4138-0063|FD-Score:2.95|P-value:8.74E-4 ID:SGTC_1747|Compound:st037496|FD-Score:-4.67|P-value:4.56E-7 ID:SGTC_1819|Compound:st053448|FD-Score:-3.63|P-value:6.93E-5 ID:SGTC_1824|Compound:st053723|FD-Score:3.74|P-value:3.65E-5 ID:SGTC_2078|Compound:5212016|FD-Score:3.78|P-value:3.12E-5 ID:SGTC_2263|Compound:7948595|FD-Score:2.96|P-value:8.32E-4 ID:SGTC_2276|Compound:7947513|FD-Score:-2.99|P-value:8.42E-4 ID:SGTC_2309|Compound:7664088|FD-Score:3.12|P-value:4.58E-4 ID:SGTC_2380|Compound:9035439|FD-Score:3.73|P-value:3.76E-5 ID:SGTC_2412|Compound:st077135|FD-Score:3.18|P-value:3.68E-4 ID:SGTC_2461|Compound:5755302|FD-Score:3.18|P-value:3.69E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:3.27|P-value:2.60E-4 ID:SGTC_2616|Compound:abietic acid|FD-Score:3.19|P-value:3.60E-4 ID:SGTC_2629|Compound:bisabolol|FD-Score:3.36|P-value:1.85E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-3.30|P-value:2.66E-4 ID:SGTC_3328|Compound:9140624|FD-Score:5.49|P-value:3.03E-9 ID:SGTC_3330|Compound:9141424|FD-Score:3.95|P-value:1.40E-5 ID:SGTC_3332|Compound:9141069|FD-Score:5.24|P-value:1.47E-8 ID:SGTC_3356|Compound:9151811|FD-Score:-3.75|P-value:3.96E-5

Top fitness defect scores for YMR162C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3328 9140624 5.49 29.0 µM 3.03E-9 6 1.98E-8
2 SGTC_3332 9141069 5.24 11.2 µM 1.47E-8 2 8.16E-8
3 SGTC_822 1487-0950 4.83 154.0 µM 1.60E-7 4 6.95E-7
4 SGTC_621 3584-0052 4.05 32.1 µM 8.81E-6 25 2.55E-5
5 SGTC_828 2074-0507 3.95 139.0 µM 1.39E-5 RPP1 & pyrimidine depletion 25 3.84E-5
6 SGTC_3330 9141424 3.95 71.4 µM 1.40E-5 amide catabolism 17 3.87E-5
7 SGTC_2078 5212016 3.78 200.0 µM 3.12E-5 60S ribosome export 21 7.96E-5
8 SGTC_1824 st053723 3.74 68.6 µM 3.65E-5 40 9.16E-5
9 SGTC_2380 9035439 3.73 200.0 µM 3.76E-5 endomembrane recycling 31 9.39E-5
10 SGTC_1221 2958-7315 3.66 257.0 µM 5.11E-5 21 1.24E-4
11 SGTC_997 3996-0078 3.40 137.0 µM 1.53E-4 46 3.32E-4
12 SGTC_72 0744-0168 3.39 198.4 µM 1.60E-4 RPP1 & pyrimidine depletion 45 3.46E-4
13 SGTC_2629 bisabolol 3.36 100.0 µM 1.85E-4 43 3.93E-4
14 SGTC_475 α-linolenic acid 3.29 10.0 µM 2.41E-4 excess fatty acid 40 5.00E-4
15 SGTC_2582 agaric acid 3.27 100.0 µM 2.60E-4 redox potentiating 391 5.34E-4
16 SGTC_2616 abietic acid 3.19 100.0 µM 3.60E-4 60 7.17E-4
17 SGTC_2412 st077135 3.18 33.4 µM 3.68E-4 57 7.31E-4
18 SGTC_2461 5755302 3.18 26.1 µM 3.69E-4 69 7.33E-4
19 SGTC_2309 7664088 3.12 163.9 µM 4.58E-4 92 8.90E-4
20 SGTC_527 4476-4215 3.07 268.0 µM 5.58E-4 PDR1 81 0.00106

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.141 2.91E-16 YNL106C INP52 hom Polyphosphatidylinositol phosphatase, dephosphorylates a number of phosphatidylinositols (PIs) to PI; involved in endocytosis; hyperosmotic stress causes translocation to actin patches; synaptojanin-like protein with a Sac1 domain
0.131 2.50E-14 YGL042C_d YGL042C_d hom Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene DST1
0.117 1.07E-11 YDR379C-A YDR379C-A hom Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; mutations in human ortholog SDHAF1 are associated with infantile leukoencephalopathy
0.114 3.75E-11 YLR241W_p YLR241W_p hom Putative protein of unknown function, may be involved in detoxification
0.108 3.71E-10 YIL129C TAO3 het Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with protein kinase Cbk1p and also with Kic1p
0.104 1.57E-9 YOR084W LPX1 hom Oleic acid-inducible, peroxisomal matrix localized lipase; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; peroxisomal import is dependent on the PTS1 receptor, Pex5p and on self-interaction
0.104 1.54E-9 YML103C NUP188 hom Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC organization and nucleocytoplasmic transport; homologous to human NUP188
0.099 9.86E-9 YFR032C-A RPL29 hom Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog
0.098 1.28E-8 YOR049C RSB1 hom Suppressor of sphingoid long chain base (LCB) sensitivity of an LCB-lyase mutation; putative integral membrane transporter or flippase that may transport LCBs from the cytoplasmic side toward the extracytoplasmic side of the membrane
0.098 1.40E-8 YNL105W_d RRT16_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene INP52; identified in a screen for mutants with decreased levels of rDNA transcription
0.097 1.75E-8 YKL123W_d YKL123W_d hom Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene SSH4
0.095 3.06E-8 YML052W SUR7 hom Plasma membrane protein that localizes to furrow-like invaginations (MCC patches); component of eisosomes; associated with endocytosis, along with Pil1p and Lsp1p; sporulation and plasma membrane sphingolipid content are altered in mutants
0.095 3.81E-8 YNL103W MET4 het Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p
0.094 4.63E-8 YOR299W BUD7 hom Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BUD7 has a paralog, BCH1, that arose from the whole genome duplication
0.093 6.16E-8 YBL096C_d YBL096C_d hom Non-essential protein of unknown function