Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; YMR178W is not an essential gene; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
Profile for YMR178W / YMR178W

Click on Significant Values for Screen Details

Top fitness defect scores for YMR178W deletion by condition

RankScreen IDConditionFD ScoreConc
1 SGTC_2629 bisabolol 7.58 100.0 µM 1.71E-16 3 1.71E-14
2 SGTC_1758 st042942 5.81 75.2 µM 2.02E-10 2 3.17E-9
3 SGTC_2437 5741531 5.35 144.2 µM 4.21E-9 14 4.44E-8
4 SGTC_1815 st051655 5.28 58.2 µM 6.64E-9 TSC3-RPN4 3 6.60E-8
5 SGTC_1853 st056710 4.93 91.2 µM 5.31E-8 amide catabolism 9 4.01E-7
6 SGTC_2931 9014674 4.85 55.7 µM 8.93E-8 7 6.31E-7
7 SGTC_940 3232-1287 4.73 54.8 µM 1.77E-7 NEO1 19 1.14E-6
8 SGTC_2817 7995342 4.30 71.4 µM 1.78E-6 17 8.49E-6
9 SGTC_1856 st057054 4.29 55.7 µM 1.85E-6 20 8.81E-6
10 SGTC_837 0091-0265 4.27 12.5 µM 2.05E-6 ubiquinone biosynthesis & proteasome 20 9.63E-6
11 SGTC_2955 9076254 4.14 12.2 µM 4.08E-6 13 1.75E-5
12 SGTC_2947 9072639 4.09 7.7 µM 5.15E-6 tubulin folding & SWR complex 39 2.14E-5
13 SGTC_2416 tofa 4.02 880.0 nM 7.32E-6 sphingolipid biosynthesis & PDR1 48 2.91E-5
14 SGTC_1457 k015-0032 3.94 57.3 µM 1.10E-5 35 4.15E-5
15 SGTC_745 0469-0705 3.88 296.0 µM 1.44E-5 mitochondrial processes 24 5.23E-5
16 SGTC_2252 omeprazole 3.84 400.0 µM 1.70E-5 11 6.05E-5
17 SGTC_1006 4099-6547 3.59 255.0 µM 5.43E-5 33 1.67E-4
18 SGTC_1750 st081859 3.58 2.4 µM 5.72E-5 endomembrane recycling 215 1.74E-4
19 SGTC_2255 7941087 3.54 197.4 µM 6.82E-5 43 2.03E-4
20 SGTC_1954 st077032 3.50 68.9 µM 8.07E-5 35 2.35E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.134 7.40E-15 YKR028W SAP190 hom Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap185p; SAP190 has a paralog, SAP185, that arose from the whole genome duplication
0.129 8.00E-14 YNL181W_p YNL181W_p het Putative oxidoreductase; required for cell viability
0.117 1.15E-11 YOR078W BUD21 hom Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern
0.108 3.14E-10 YMR126C_p DLT1_p hom Protein of unknown function, mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA)
0.106 8.71E-10 YLR420W URA4 hom Dihydroorotase, catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate
0.096 2.68E-8 YPR029C APL4 hom Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport
0.095 3.22E-8 YKL098W MTC2 hom Protein of unknown function; mtc2 is synthetically sick with cdc13-1
0.093 7.41E-8 YOL084W PHM7 hom Protein of unknown function; expression is regulated by phosphate levels; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; protein abundance increases in response to DNA replication stress
0.092 9.60E-8 YNL327W EGT2 hom Glycosylphosphatidylinositol (GPI)-anchored cell wall endoglucanase required for proper cell separation after cytokinesis, expression is activated by Swi5p and tightly regulated in a cell cycle-dependent manner
0.088 3.67E-7 YPR153W YPR153W hom Putative protein of unknown function
0.087 4.23E-7 YNL269W BSC4 hom Protein of unknown function, ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough is increased upon depletion of Sup35p
0.085 7.49E-7 YCL046W_d YCL046W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C
0.085 8.60E-7 YJL138C TIF2 hom Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication
0.082 2.18E-6 YGR086C PIL1 hom Primary protein component of eisosomes; eisosomes are large immobile cell cortex structures associated with endocytosis; null mutant shows activation of Pkc1p/Ypk1p stress resistance pathways; detected in phosphorylated state in mitochondria; member of the BAR domain family; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress
0.080 3.66E-6 YGR037C ACB1 hom Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress