YMR194C-B / CMC4

Protein that localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; contains twin cysteine-x(9)-cysteine motifs

Zygosity: Homozygous strain
fixedexpanded
Profile for YMR194C-B / CMC4

Click on Significant Values for Screen Details ID:SGTC_123|Compound:0083-0120|FD-Score:4.05|P-value:5.89E-6 ID:SGTC_161|Compound:4065-0411|FD-Score:2.89|P-value:8.64E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:-2.91|P-value:7.94E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:5.25|P-value:6.51E-9 ID:SGTC_436|Compound:n,n-dimethylsphingosine|FD-Score:7.08|P-value:8.63E-15 ID:SGTC_468|Compound:gf-109203x|FD-Score:3.11|P-value:3.83E-4 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:3.22|P-value:2.46E-4 ID:SGTC_624|Compound:k048-0052|FD-Score:-3.92|P-value:1.09E-5 ID:SGTC_662|Compound:k832-1615|FD-Score:3.07|P-value:4.49E-4 ID:SGTC_734|Compound:2023-0417|FD-Score:3.10|P-value:3.98E-4 ID:SGTC_751|Compound:0833-0568|FD-Score:4.18|P-value:3.06E-6 ID:SGTC_760|Compound:0568-0725|FD-Score:-3.19|P-value:2.74E-4 ID:SGTC_906|Compound:1165-0455|FD-Score:2.97|P-value:6.43E-4 ID:SGTC_975|Compound:1335-0046|FD-Score:2.97|P-value:6.59E-4 ID:SGTC_1045|Compound:1348-1485|FD-Score:3.63|P-value:4.25E-5 ID:SGTC_1369|Compound:1866-0067|FD-Score:2.86|P-value:9.93E-4 ID:SGTC_1610|Compound:st001799|FD-Score:-3.60|P-value:4.86E-5 ID:SGTC_1613|Compound:st002381|FD-Score:3.17|P-value:2.94E-4 ID:SGTC_1620|Compound:st003704|FD-Score:3.64|P-value:3.99E-5 ID:SGTC_1646|Compound:st011709|FD-Score:-3.83|P-value:1.66E-5 ID:SGTC_1748|Compound:st037455|FD-Score:3.34|P-value:1.47E-4 ID:SGTC_1758|Compound:st042942|FD-Score:4.50|P-value:5.39E-7 ID:SGTC_1762|Compound:st045503|FD-Score:6.19|P-value:1.01E-11 ID:SGTC_1763|Compound:st044482|FD-Score:-3.15|P-value:3.18E-4 ID:SGTC_1764|Compound:st044502|FD-Score:3.42|P-value:1.08E-4 ID:SGTC_1836|Compound:st055642|FD-Score:3.69|P-value:3.21E-5 ID:SGTC_1841|Compound:st054148|FD-Score:-3.06|P-value:4.53E-4 ID:SGTC_1920|Compound:st075728|FD-Score:2.90|P-value:8.63E-4 ID:SGTC_1938|Compound:st074717|FD-Score:3.72|P-value:2.81E-5 ID:SGTC_1943|Compound:st076331|FD-Score:-2.92|P-value:7.74E-4 ID:SGTC_1971|Compound:st069429|FD-Score:2.96|P-value:6.65E-4 ID:SGTC_2035|Compound:5154463|FD-Score:2.98|P-value:6.37E-4 ID:SGTC_2260|Compound:7964312|FD-Score:3.03|P-value:5.27E-4 ID:SGTC_2285|Compound:7945142|FD-Score:4.02|P-value:6.84E-6 ID:SGTC_2313|Compound:7726885|FD-Score:-3.02|P-value:5.41E-4 ID:SGTC_2326|Compound:6164455|FD-Score:-3.86|P-value:1.43E-5 ID:SGTC_2334|Compound:9017922|FD-Score:-3.06|P-value:4.54E-4 ID:SGTC_2407|Compound:paclitaxel|FD-Score:4.90|P-value:5.62E-8 ID:SGTC_2788|Compound:5282734|FD-Score:4.03|P-value:6.41E-6 ID:SGTC_2999|Compound:9069765|FD-Score:3.83|P-value:1.69E-5 ID:SGTC_3219|Compound:9131112|FD-Score:3.03|P-value:5.20E-4 ID:SGTC_123|Compound:0083-0120|FD-Score:4.05|P-value:5.89E-6 ID:SGTC_161|Compound:4065-0411|FD-Score:2.89|P-value:8.64E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:-2.91|P-value:7.94E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:5.25|P-value:6.51E-9 ID:SGTC_436|Compound:n,n-dimethylsphingosine|FD-Score:7.08|P-value:8.63E-15 ID:SGTC_468|Compound:gf-109203x|FD-Score:3.11|P-value:3.83E-4 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:3.22|P-value:2.46E-4 ID:SGTC_624|Compound:k048-0052|FD-Score:-3.92|P-value:1.09E-5 ID:SGTC_662|Compound:k832-1615|FD-Score:3.07|P-value:4.49E-4 ID:SGTC_734|Compound:2023-0417|FD-Score:3.10|P-value:3.98E-4 ID:SGTC_751|Compound:0833-0568|FD-Score:4.18|P-value:3.06E-6 ID:SGTC_760|Compound:0568-0725|FD-Score:-3.19|P-value:2.74E-4 ID:SGTC_906|Compound:1165-0455|FD-Score:2.97|P-value:6.43E-4 ID:SGTC_975|Compound:1335-0046|FD-Score:2.97|P-value:6.59E-4 ID:SGTC_1045|Compound:1348-1485|FD-Score:3.63|P-value:4.25E-5 ID:SGTC_1369|Compound:1866-0067|FD-Score:2.86|P-value:9.93E-4 ID:SGTC_1610|Compound:st001799|FD-Score:-3.60|P-value:4.86E-5 ID:SGTC_1613|Compound:st002381|FD-Score:3.17|P-value:2.94E-4 ID:SGTC_1620|Compound:st003704|FD-Score:3.64|P-value:3.99E-5 ID:SGTC_1646|Compound:st011709|FD-Score:-3.83|P-value:1.66E-5 ID:SGTC_1748|Compound:st037455|FD-Score:3.34|P-value:1.47E-4 ID:SGTC_1758|Compound:st042942|FD-Score:4.50|P-value:5.39E-7 ID:SGTC_1762|Compound:st045503|FD-Score:6.19|P-value:1.01E-11 ID:SGTC_1763|Compound:st044482|FD-Score:-3.15|P-value:3.18E-4 ID:SGTC_1764|Compound:st044502|FD-Score:3.42|P-value:1.08E-4 ID:SGTC_1836|Compound:st055642|FD-Score:3.69|P-value:3.21E-5 ID:SGTC_1841|Compound:st054148|FD-Score:-3.06|P-value:4.53E-4 ID:SGTC_1920|Compound:st075728|FD-Score:2.90|P-value:8.63E-4 ID:SGTC_1938|Compound:st074717|FD-Score:3.72|P-value:2.81E-5 ID:SGTC_1943|Compound:st076331|FD-Score:-2.92|P-value:7.74E-4 ID:SGTC_1971|Compound:st069429|FD-Score:2.96|P-value:6.65E-4 ID:SGTC_2035|Compound:5154463|FD-Score:2.98|P-value:6.37E-4 ID:SGTC_2260|Compound:7964312|FD-Score:3.03|P-value:5.27E-4 ID:SGTC_2285|Compound:7945142|FD-Score:4.02|P-value:6.84E-6 ID:SGTC_2313|Compound:7726885|FD-Score:-3.02|P-value:5.41E-4 ID:SGTC_2326|Compound:6164455|FD-Score:-3.86|P-value:1.43E-5 ID:SGTC_2334|Compound:9017922|FD-Score:-3.06|P-value:4.54E-4 ID:SGTC_2407|Compound:paclitaxel|FD-Score:4.90|P-value:5.62E-8 ID:SGTC_2788|Compound:5282734|FD-Score:4.03|P-value:6.41E-6 ID:SGTC_2999|Compound:9069765|FD-Score:3.83|P-value:1.69E-5 ID:SGTC_3219|Compound:9131112|FD-Score:3.03|P-value:5.20E-4

Top fitness defect scores for YMR194C-B deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_436 n,n-dimethylsphingosine 7.08 3.3 µM 8.63E-15 1 7.08E-13
2 SGTC_1762 st045503 6.19 25.5 µM 1.01E-11 4 2.97E-10
3 SGTC_236 1509-0011 5.25 5.3 µM 6.51E-9 312 7.56E-8
4 SGTC_2407 paclitaxel 4.90 400.0 µM 5.62E-8 17 4.80E-7
5 SGTC_1758 st042942 4.50 75.2 µM 5.39E-7 11 3.33E-6
6 SGTC_751 0833-0568 4.18 350.0 µM 3.06E-6 14 1.48E-5
7 SGTC_123 0083-0120 4.05 66.7 µM 5.89E-6 44 2.60E-5
8 SGTC_2788 5282734 4.03 71.4 µM 6.41E-6 44 2.79E-5
9 SGTC_2285 7945142 4.02 200.0 µM 6.84E-6 21 2.95E-5
10 SGTC_2999 9069765 3.83 71.4 µM 1.69E-5 amide catabolism 31 6.40E-5
11 SGTC_1938 st074717 3.72 56.4 µM 2.81E-5 17 9.95E-5
12 SGTC_1836 st055642 3.69 81.0 µM 3.21E-5 23 1.12E-4
13 SGTC_1620 st003704 3.64 16.5 µM 3.99E-5 25 1.34E-4
14 SGTC_1045 1348-1485 3.63 165.0 µM 4.25E-5 46 1.42E-4
15 SGTC_1764 st044502 3.42 93.5 µM 1.08E-4 39 3.16E-4
16 SGTC_1748 st037455 3.34 53.6 µM 1.47E-4 56 4.13E-4
17 SGTC_474 dl-dihydrosphingosine 3.22 5.0 µM 2.46E-4 65 6.42E-4
18 SGTC_1613 st002381 3.17 52.1 µM 2.94E-4 47 7.50E-4
19 SGTC_468 gf-109203x 3.11 121.0 µM 3.83E-4 amide catabolism 16 9.41E-4
20 SGTC_734 2023-0417 3.10 80.0 µM 3.98E-4 ergosterol biosynthesis 55 9.72E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.191 5.59E-29 YOR330C MIP1 hom Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit
0.191 7.19E-29 YGL237C HAP2 hom Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding
0.182 1.82E-26 YJR120W YJR120W hom Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p
0.176 7.08E-25 YJL166W QCR8 hom Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
0.174 2.51E-24 YHR050W-A_p YHR050W-A_p hom Protein of unknown function; identified by expression profiling and mass spectrometry
0.171 2.64E-23 YMR035W IMP2 hom Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p
0.170 3.21E-23 YKL109W HAP4 hom Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex
0.167 2.18E-22 YDR230W_d YDR230W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20
0.165 8.81E-22 YKL087C CYT2 hom Cytochrome c1 heme lyase, involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1
0.162 4.38E-21 YMR286W MRPL33 hom Mitochondrial ribosomal protein of the large subunit
0.162 4.19E-21 YPL215W CBP3 hom Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.160 1.02E-20 YDR518W EUG1 hom Protein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER
0.157 4.87E-20 YER141W COX15 hom Protein required for the hydroxylation of heme O to form heme A, which is an essential prosthetic group for cytochrome c oxidase
0.155 1.34E-19 YNL052W COX5A hom Subunit Va of cytochrome c oxidase; cytochrome c oxidase is the terminal member of the mitochondrial inner membrane electron transport chain; Cox5Ap is predominantly expressed during aerobic growth while its isoform Vb (Cox5Bp) is expressed during anaerobic growth; COX5A has a paralog, COX5B, that arose from the whole genome duplication
0.155 1.95E-19 YOR065W CYT1 hom Cytochrome c1, component of the mitochondrial respiratory chain; expression is regulated by the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex