YMR205C / PFK2

Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes

Zygosity: Homozygous strain
fixedexpanded
Profile for YMR205C / PFK2

Click on Significant Values for Screen Details ID:SGTC_25|Compound:0118-0223|FD-Score:4.43|P-value:2.45E-5 ID:SGTC_49|Compound:0717-0965|FD-Score:3.77|P-value:3.25E-4 ID:SGTC_69|Compound:0096-0274|FD-Score:4.27|P-value:4.72E-5 ID:SGTC_197|Compound:4544-0043|FD-Score:3.47|P-value:9.20E-4 ID:SGTC_209|Compound:4077-0173|FD-Score:3.76|P-value:3.38E-4 ID:SGTC_378|Compound:3970-0959|FD-Score:3.65|P-value:4.98E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:-2.83|P-value:9.39E-4 ID:SGTC_429|Compound:1112-0049|FD-Score:-3.98|P-value:1.12E-5 ID:SGTC_662|Compound:k832-1615|FD-Score:-3.25|P-value:2.12E-4 ID:SGTC_766|Compound:1391-0721|FD-Score:3.55|P-value:7.09E-4 ID:SGTC_811|Compound:0416-0114|FD-Score:3.88|P-value:2.15E-4 ID:SGTC_893|Compound:0958-0369|FD-Score:-3.23|P-value:2.35E-4 ID:SGTC_960|Compound:1222-0186|FD-Score:3.45|P-value:9.87E-4 ID:SGTC_964|Compound:1287-0107|FD-Score:3.55|P-value:7.08E-4 ID:SGTC_1007|Compound:4101-0031|FD-Score:-2.97|P-value:5.98E-4 ID:SGTC_1014|Compound:4130-3005|FD-Score:3.72|P-value:3.86E-4 ID:SGTC_1032|Compound:k061-1018|FD-Score:3.46|P-value:9.49E-4 ID:SGTC_1219|Compound:1469-0211|FD-Score:-2.83|P-value:9.59E-4 ID:SGTC_1447|Compound:4239-0816|FD-Score:3.76|P-value:3.37E-4 ID:SGTC_1452|Compound:4194-0072|FD-Score:3.68|P-value:4.46E-4 ID:SGTC_1455|Compound:4262-0120|FD-Score:3.70|P-value:4.13E-4 ID:SGTC_1513|Compound:0337-0559|FD-Score:3.81|P-value:2.79E-4 ID:SGTC_1528|Compound:arabinosylhypoxanthine|FD-Score:3.82|P-value:2.66E-4 ID:SGTC_1616|Compound:st002734|FD-Score:-3.34|P-value:1.52E-4 ID:SGTC_1646|Compound:st011709|FD-Score:-4.42|P-value:1.47E-6 ID:SGTC_1672|Compound:st016598|FD-Score:-3.16|P-value:3.01E-4 ID:SGTC_1710|Compound:st031758|FD-Score:3.63|P-value:5.32E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:-2.86|P-value:8.63E-4 ID:SGTC_1907|Compound:st060210|FD-Score:3.49|P-value:8.68E-4 ID:SGTC_1939|Compound:st074455|FD-Score:3.66|P-value:4.77E-4 ID:SGTC_2034|Compound:5197015|FD-Score:3.62|P-value:5.53E-4 ID:SGTC_2137|Compound:5261020|FD-Score:3.51|P-value:8.09E-4 ID:SGTC_2164|Compound:5685496|FD-Score:3.48|P-value:8.97E-4 ID:SGTC_2229|Compound:7354935|FD-Score:-3.55|P-value:6.90E-5 ID:SGTC_2271|Compound:7849410|FD-Score:-3.02|P-value:4.94E-4 ID:SGTC_2274|Compound:7947094|FD-Score:3.84|P-value:2.49E-4 ID:SGTC_2279|Compound:7948423|FD-Score:3.58|P-value:6.31E-4 ID:SGTC_2376|Compound:9072922|FD-Score:3.67|P-value:4.67E-4 ID:SGTC_2472|Compound:5377652|FD-Score:3.66|P-value:4.84E-4 ID:SGTC_2481|Compound:5478948|FD-Score:3.91|P-value:1.97E-4 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:3.58|P-value:6.35E-4 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:3.58|P-value:6.41E-4 ID:SGTC_2674|Compound:ligustilide|FD-Score:3.46|P-value:9.59E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:-3.23|P-value:2.35E-4 ID:SGTC_2703|Compound:st077852|FD-Score:3.85|P-value:2.40E-4 ID:SGTC_2712|Compound:st079126|FD-Score:3.46|P-value:9.39E-4 ID:SGTC_2772|Compound:vinpocetine|FD-Score:4.11|P-value:8.90E-5 ID:SGTC_2835|Compound:9006588|FD-Score:3.85|P-value:2.44E-4 ID:SGTC_2894|Compound:9048625|FD-Score:3.67|P-value:4.58E-4 ID:SGTC_2935|Compound:9038016|FD-Score:-2.89|P-value:7.80E-4 ID:SGTC_2938|Compound:9059746|FD-Score:3.54|P-value:7.25E-4 ID:SGTC_2998|Compound:9066762|FD-Score:-3.03|P-value:4.84E-4 ID:SGTC_3049|Compound:9092223|FD-Score:-2.92|P-value:7.03E-4 ID:SGTC_3097|Compound:9117246|FD-Score:3.46|P-value:9.50E-4 ID:SGTC_3131|Compound:9127911|FD-Score:4.06|P-value:1.08E-4 ID:SGTC_3132|Compound:9126706|FD-Score:4.00|P-value:1.37E-4 ID:SGTC_3197|Compound:9113331|FD-Score:-3.37|P-value:1.37E-4 ID:SGTC_3207|Compound:9114163|FD-Score:3.48|P-value:8.92E-4 ID:SGTC_3211|Compound:9128781|FD-Score:3.48|P-value:8.79E-4 ID:SGTC_25|Compound:0118-0223|FD-Score:4.43|P-value:2.45E-5 ID:SGTC_49|Compound:0717-0965|FD-Score:3.77|P-value:3.25E-4 ID:SGTC_69|Compound:0096-0274|FD-Score:4.27|P-value:4.72E-5 ID:SGTC_197|Compound:4544-0043|FD-Score:3.47|P-value:9.20E-4 ID:SGTC_209|Compound:4077-0173|FD-Score:3.76|P-value:3.38E-4 ID:SGTC_378|Compound:3970-0959|FD-Score:3.65|P-value:4.98E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:-2.83|P-value:9.39E-4 ID:SGTC_429|Compound:1112-0049|FD-Score:-3.98|P-value:1.12E-5 ID:SGTC_662|Compound:k832-1615|FD-Score:-3.25|P-value:2.12E-4 ID:SGTC_766|Compound:1391-0721|FD-Score:3.55|P-value:7.09E-4 ID:SGTC_811|Compound:0416-0114|FD-Score:3.88|P-value:2.15E-4 ID:SGTC_893|Compound:0958-0369|FD-Score:-3.23|P-value:2.35E-4 ID:SGTC_960|Compound:1222-0186|FD-Score:3.45|P-value:9.87E-4 ID:SGTC_964|Compound:1287-0107|FD-Score:3.55|P-value:7.08E-4 ID:SGTC_1007|Compound:4101-0031|FD-Score:-2.97|P-value:5.98E-4 ID:SGTC_1014|Compound:4130-3005|FD-Score:3.72|P-value:3.86E-4 ID:SGTC_1032|Compound:k061-1018|FD-Score:3.46|P-value:9.49E-4 ID:SGTC_1219|Compound:1469-0211|FD-Score:-2.83|P-value:9.59E-4 ID:SGTC_1447|Compound:4239-0816|FD-Score:3.76|P-value:3.37E-4 ID:SGTC_1452|Compound:4194-0072|FD-Score:3.68|P-value:4.46E-4 ID:SGTC_1455|Compound:4262-0120|FD-Score:3.70|P-value:4.13E-4 ID:SGTC_1513|Compound:0337-0559|FD-Score:3.81|P-value:2.79E-4 ID:SGTC_1528|Compound:arabinosylhypoxanthine|FD-Score:3.82|P-value:2.66E-4 ID:SGTC_1616|Compound:st002734|FD-Score:-3.34|P-value:1.52E-4 ID:SGTC_1646|Compound:st011709|FD-Score:-4.42|P-value:1.47E-6 ID:SGTC_1672|Compound:st016598|FD-Score:-3.16|P-value:3.01E-4 ID:SGTC_1710|Compound:st031758|FD-Score:3.63|P-value:5.32E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:-2.86|P-value:8.63E-4 ID:SGTC_1907|Compound:st060210|FD-Score:3.49|P-value:8.68E-4 ID:SGTC_1939|Compound:st074455|FD-Score:3.66|P-value:4.77E-4 ID:SGTC_2034|Compound:5197015|FD-Score:3.62|P-value:5.53E-4 ID:SGTC_2137|Compound:5261020|FD-Score:3.51|P-value:8.09E-4 ID:SGTC_2164|Compound:5685496|FD-Score:3.48|P-value:8.97E-4 ID:SGTC_2229|Compound:7354935|FD-Score:-3.55|P-value:6.90E-5 ID:SGTC_2271|Compound:7849410|FD-Score:-3.02|P-value:4.94E-4 ID:SGTC_2274|Compound:7947094|FD-Score:3.84|P-value:2.49E-4 ID:SGTC_2279|Compound:7948423|FD-Score:3.58|P-value:6.31E-4 ID:SGTC_2376|Compound:9072922|FD-Score:3.67|P-value:4.67E-4 ID:SGTC_2472|Compound:5377652|FD-Score:3.66|P-value:4.84E-4 ID:SGTC_2481|Compound:5478948|FD-Score:3.91|P-value:1.97E-4 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:3.58|P-value:6.35E-4 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:3.58|P-value:6.41E-4 ID:SGTC_2674|Compound:ligustilide|FD-Score:3.46|P-value:9.59E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:-3.23|P-value:2.35E-4 ID:SGTC_2703|Compound:st077852|FD-Score:3.85|P-value:2.40E-4 ID:SGTC_2712|Compound:st079126|FD-Score:3.46|P-value:9.39E-4 ID:SGTC_2772|Compound:vinpocetine|FD-Score:4.11|P-value:8.90E-5 ID:SGTC_2835|Compound:9006588|FD-Score:3.85|P-value:2.44E-4 ID:SGTC_2894|Compound:9048625|FD-Score:3.67|P-value:4.58E-4 ID:SGTC_2935|Compound:9038016|FD-Score:-2.89|P-value:7.80E-4 ID:SGTC_2938|Compound:9059746|FD-Score:3.54|P-value:7.25E-4 ID:SGTC_2998|Compound:9066762|FD-Score:-3.03|P-value:4.84E-4 ID:SGTC_3049|Compound:9092223|FD-Score:-2.92|P-value:7.03E-4 ID:SGTC_3097|Compound:9117246|FD-Score:3.46|P-value:9.50E-4 ID:SGTC_3131|Compound:9127911|FD-Score:4.06|P-value:1.08E-4 ID:SGTC_3132|Compound:9126706|FD-Score:4.00|P-value:1.37E-4 ID:SGTC_3197|Compound:9113331|FD-Score:-3.37|P-value:1.37E-4 ID:SGTC_3207|Compound:9114163|FD-Score:3.48|P-value:8.92E-4 ID:SGTC_3211|Compound:9128781|FD-Score:3.48|P-value:8.79E-4

Top fitness defect scores for YMR205C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_25 0118-0223 4.43 35.7 µM 2.45E-5 8 4.74E-6
2 SGTC_69 0096-0274 4.27 206.7 µM 4.72E-5 mitochondrial processes 30 9.75E-6
3 SGTC_2772 vinpocetine 4.11 57.1 µM 8.90E-5 19 1.96E-5
4 SGTC_3131 9127911 4.06 49.5 µM 1.08E-4 26 2.42E-5
5 SGTC_3132 9126706 4.00 49.5 µM 1.37E-4 21 3.14E-5
6 SGTC_2481 5478948 3.91 45.2 µM 1.97E-4 PDR1 36 4.71E-5
7 SGTC_811 0416-0114 3.88 265.0 µM 2.15E-4 25 5.18E-5
8 SGTC_2703 st077852 3.85 53.4 µM 2.40E-4 27 5.84E-5
9 SGTC_2835 9006588 3.85 71.4 µM 2.44E-4 44 5.96E-5
10 SGTC_2274 7947094 3.84 46.1 µM 2.49E-4 40 6.09E-5
11 SGTC_1528 arabinosylhypoxanthine 3.82 74.6 µM 2.66E-4 27 6.56E-5
12 SGTC_1513 0337-0559 3.81 19.0 µM 2.79E-4 21 6.91E-5
13 SGTC_49 0717-0965 3.77 32.1 µM 3.25E-4 60S ribosome export 25 8.17E-5
14 SGTC_1447 4239-0816 3.76 81.9 µM 3.37E-4 35 8.51E-5
15 SGTC_209 4077-0173 3.76 175.6 µM 3.38E-4 12 8.54E-5
16 SGTC_1014 4130-3005 3.72 69.3 µM 3.86E-4 25 9.90E-5
17 SGTC_1455 4262-0120 3.70 131.0 µM 4.13E-4 plasma membrane duress 27 1.07E-4
18 SGTC_1452 4194-0072 3.68 190.0 µM 4.46E-4 19 1.16E-4
19 SGTC_2894 9048625 3.67 32.5 µM 4.58E-4 18 1.20E-4
20 SGTC_2376 9072922 3.67 81.3 µM 4.67E-4 41 1.22E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.122 1.25E-12 YJL138C TIF2 hom Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication
0.098 1.36E-8 YOR336W KRE5 het Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects
0.085 8.33E-7 YDR074W TPS2 hom Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress
0.085 9.30E-7 YNR023W SNF12 hom 73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive
0.084 1.13E-6 YIL087C AIM19 hom Putative protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays reduced respiratory growth
0.073 2.27E-5 YOL091W SPO21 hom Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation
0.073 2.55E-5 YBL050W SEC17 het Peripheral membrane protein required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, and autophagy; stimulates the ATPase activity of Sec18p; has similarity to mammalian alpha-SNAP
0.072 3.26E-5 YDR536W STL1 hom Glycerol proton symporter of the plasma membrane, subject to glucose-induced inactivation, strongly but transiently induced when cells are subjected to osmotic shock
0.071 3.56E-5 YGL038C OCH1 hom Mannosyltransferase of the cis-Golgi apparatus, initiates the polymannose outer chain elongation of N-linked oligosaccharides of glycoproteins
0.069 6.18E-5 YNL296W_d YNL296W_d hom Dubious open reading frame unlikely to encode a functional protein; deletion adversely affects sporulation; deletion mutant exhibits synthetic phenotype under expression of mutant huntingtin fragment, but gene does not have human ortholog
0.069 6.89E-5 YPR134W MSS18 hom Nuclear encoded protein needed for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions
0.067 1.16E-4 YOR208W PTP2 hom Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the nucleus
0.066 1.38E-4 YPR122W AXL1 hom Haploid specific endoprotease that performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells
0.066 1.38E-4 YML032C RAD52 hom Protein that stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA; anneals complementary single-stranded DNA; involved in the repair of double-strand breaks in DNA during vegetative growth and meiosis
0.065 1.51E-4 YOR268C_p YOR268C_p hom Putative protein of unknown function; sporulation is abnormal in homozygous diploid; YOR268C is not an essential gene