YMR255W / GFD1

Coiled-coiled protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YMR255W / GFD1

Click on Significant Values for Screen Details ID:SGTC_211|Compound:3455-0679|FD-Score:3.41|P-value:1.98E-4 ID:SGTC_375|Compound:3970-0961|FD-Score:4.06|P-value:1.24E-5 ID:SGTC_683|Compound:panaxydol|FD-Score:4.50|P-value:1.48E-6 ID:SGTC_731|Compound:3966-0296|FD-Score:3.30|P-value:3.02E-4 ID:SGTC_886|Compound:0866-0150|FD-Score:3.28|P-value:3.35E-4 ID:SGTC_953|Compound:1188-0045|FD-Score:4.42|P-value:2.23E-6 ID:SGTC_961|Compound:1252-1571|FD-Score:-4.84|P-value:2.56E-7 ID:SGTC_963|Compound:1269-2853|FD-Score:-8.09|P-value:2.28E-17 ID:SGTC_1029|Compound:k015-0030|FD-Score:-3.19|P-value:4.62E-4 ID:SGTC_1040|Compound:k898-0338|FD-Score:-3.24|P-value:3.93E-4 ID:SGTC_1097|Compound:itavastatin|FD-Score:3.56|P-value:1.09E-4 ID:SGTC_1140|Compound:3229-1848|FD-Score:3.46|P-value:1.62E-4 ID:SGTC_1165|Compound:k072-0275|FD-Score:3.77|P-value:4.66E-5 ID:SGTC_1215|Compound:3852-0577|FD-Score:3.23|P-value:3.98E-4 ID:SGTC_1245|Compound:0418-0076|FD-Score:6.36|P-value:1.99E-11 ID:SGTC_1288|Compound:0973-0013|FD-Score:-3.39|P-value:2.17E-4 ID:SGTC_1327|Compound:1436-0082|FD-Score:3.01|P-value:8.78E-4 ID:SGTC_1392|Compound:3455-1037|FD-Score:-3.39|P-value:2.20E-4 ID:SGTC_1395|Compound:3486-0190|FD-Score:3.59|P-value:9.62E-5 ID:SGTC_1468|Compound:k284-1086|FD-Score:3.12|P-value:6.06E-4 ID:SGTC_1469|Compound:k284-2957|FD-Score:3.65|P-value:7.59E-5 ID:SGTC_1489|Compound:4065-0444|FD-Score:3.04|P-value:8.07E-4 ID:SGTC_1556|Compound:plumbagin|FD-Score:4.10|P-value:1.06E-5 ID:SGTC_1557|Compound:sumaresinolic acid|FD-Score:3.59|P-value:9.83E-5 ID:SGTC_1570|Compound:verbenone|FD-Score:6.33|P-value:2.49E-11 ID:SGTC_1607|Compound:st000305|FD-Score:-3.25|P-value:3.76E-4 ID:SGTC_1666|Compound:st014829|FD-Score:3.01|P-value:8.79E-4 ID:SGTC_1906|Compound:st060206|FD-Score:3.47|P-value:1.55E-4 ID:SGTC_1920|Compound:st075728|FD-Score:3.14|P-value:5.69E-4 ID:SGTC_2053|Compound:5265679|FD-Score:-3.12|P-value:6.03E-4 ID:SGTC_2200|Compound:6600604|FD-Score:3.05|P-value:7.79E-4 ID:SGTC_2297|Compound:7971671|FD-Score:-4.31|P-value:3.86E-6 ID:SGTC_2311|Compound:7694451|FD-Score:3.02|P-value:8.49E-4 ID:SGTC_2344|Compound:7990960|FD-Score:-3.26|P-value:3.62E-4 ID:SGTC_2453|Compound:5731132|FD-Score:4.79|P-value:3.28E-7 ID:SGTC_2474|Compound:5321570|FD-Score:3.74|P-value:5.11E-5 ID:SGTC_2576|Compound:guaiol|FD-Score:3.10|P-value:6.42E-4 ID:SGTC_2593|Compound:nobiletin|FD-Score:-3.19|P-value:4.66E-4 ID:SGTC_2771|Compound:fluoxetine|FD-Score:3.94|P-value:2.20E-5 ID:SGTC_2774|Compound:tamoxifen|FD-Score:-3.85|P-value:3.17E-5 ID:SGTC_2886|Compound:9058944|FD-Score:3.18|P-value:4.80E-4 ID:SGTC_2994|Compound:9053520|FD-Score:3.17|P-value:4.92E-4 ID:SGTC_3007|Compound:9078383|FD-Score:3.26|P-value:3.52E-4 ID:SGTC_3049|Compound:9092223|FD-Score:3.06|P-value:7.51E-4 ID:SGTC_211|Compound:3455-0679|FD-Score:3.41|P-value:1.98E-4 ID:SGTC_375|Compound:3970-0961|FD-Score:4.06|P-value:1.24E-5 ID:SGTC_683|Compound:panaxydol|FD-Score:4.50|P-value:1.48E-6 ID:SGTC_731|Compound:3966-0296|FD-Score:3.30|P-value:3.02E-4 ID:SGTC_886|Compound:0866-0150|FD-Score:3.28|P-value:3.35E-4 ID:SGTC_953|Compound:1188-0045|FD-Score:4.42|P-value:2.23E-6 ID:SGTC_961|Compound:1252-1571|FD-Score:-4.84|P-value:2.56E-7 ID:SGTC_963|Compound:1269-2853|FD-Score:-8.09|P-value:2.28E-17 ID:SGTC_1029|Compound:k015-0030|FD-Score:-3.19|P-value:4.62E-4 ID:SGTC_1040|Compound:k898-0338|FD-Score:-3.24|P-value:3.93E-4 ID:SGTC_1097|Compound:itavastatin|FD-Score:3.56|P-value:1.09E-4 ID:SGTC_1140|Compound:3229-1848|FD-Score:3.46|P-value:1.62E-4 ID:SGTC_1165|Compound:k072-0275|FD-Score:3.77|P-value:4.66E-5 ID:SGTC_1215|Compound:3852-0577|FD-Score:3.23|P-value:3.98E-4 ID:SGTC_1245|Compound:0418-0076|FD-Score:6.36|P-value:1.99E-11 ID:SGTC_1288|Compound:0973-0013|FD-Score:-3.39|P-value:2.17E-4 ID:SGTC_1327|Compound:1436-0082|FD-Score:3.01|P-value:8.78E-4 ID:SGTC_1392|Compound:3455-1037|FD-Score:-3.39|P-value:2.20E-4 ID:SGTC_1395|Compound:3486-0190|FD-Score:3.59|P-value:9.62E-5 ID:SGTC_1468|Compound:k284-1086|FD-Score:3.12|P-value:6.06E-4 ID:SGTC_1469|Compound:k284-2957|FD-Score:3.65|P-value:7.59E-5 ID:SGTC_1489|Compound:4065-0444|FD-Score:3.04|P-value:8.07E-4 ID:SGTC_1556|Compound:plumbagin|FD-Score:4.10|P-value:1.06E-5 ID:SGTC_1557|Compound:sumaresinolic acid|FD-Score:3.59|P-value:9.83E-5 ID:SGTC_1570|Compound:verbenone|FD-Score:6.33|P-value:2.49E-11 ID:SGTC_1607|Compound:st000305|FD-Score:-3.25|P-value:3.76E-4 ID:SGTC_1666|Compound:st014829|FD-Score:3.01|P-value:8.79E-4 ID:SGTC_1906|Compound:st060206|FD-Score:3.47|P-value:1.55E-4 ID:SGTC_1920|Compound:st075728|FD-Score:3.14|P-value:5.69E-4 ID:SGTC_2053|Compound:5265679|FD-Score:-3.12|P-value:6.03E-4 ID:SGTC_2200|Compound:6600604|FD-Score:3.05|P-value:7.79E-4 ID:SGTC_2297|Compound:7971671|FD-Score:-4.31|P-value:3.86E-6 ID:SGTC_2311|Compound:7694451|FD-Score:3.02|P-value:8.49E-4 ID:SGTC_2344|Compound:7990960|FD-Score:-3.26|P-value:3.62E-4 ID:SGTC_2453|Compound:5731132|FD-Score:4.79|P-value:3.28E-7 ID:SGTC_2474|Compound:5321570|FD-Score:3.74|P-value:5.11E-5 ID:SGTC_2576|Compound:guaiol|FD-Score:3.10|P-value:6.42E-4 ID:SGTC_2593|Compound:nobiletin|FD-Score:-3.19|P-value:4.66E-4 ID:SGTC_2771|Compound:fluoxetine|FD-Score:3.94|P-value:2.20E-5 ID:SGTC_2774|Compound:tamoxifen|FD-Score:-3.85|P-value:3.17E-5 ID:SGTC_2886|Compound:9058944|FD-Score:3.18|P-value:4.80E-4 ID:SGTC_2994|Compound:9053520|FD-Score:3.17|P-value:4.92E-4 ID:SGTC_3007|Compound:9078383|FD-Score:3.26|P-value:3.52E-4 ID:SGTC_3049|Compound:9092223|FD-Score:3.06|P-value:7.51E-4

Top fitness defect scores for YMR255W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1245 0418-0076 6.36 17.2 µM 1.99E-11 ERG2 3 9.83E-11
2 SGTC_1570 verbenone 6.33 133.0 µM 2.49E-11 2 1.21E-10
3 SGTC_2453 5731132 4.79 22.2 µM 3.28E-7 7 8.24E-7
4 SGTC_683 panaxydol 4.50 45.0 µM 1.48E-6 sphingolipid biosynthesis & PDR1 22 3.34E-6
5 SGTC_953 1188-0045 4.42 9.7 µM 2.23E-6 60S ribosome export 18 4.89E-6
6 SGTC_1556 plumbagin 4.10 1.5 µM 1.06E-5 superoxide 18 2.09E-5
7 SGTC_375 3970-0961 4.06 9.3 µM 1.24E-5 tubulin folding & SWR complex 16 2.43E-5
8 SGTC_2771 fluoxetine 3.94 41.0 µM 2.20E-5 22 4.14E-5
9 SGTC_1165 k072-0275 3.77 30.3 µM 4.66E-5 48 8.32E-5
10 SGTC_2474 5321570 3.74 43.3 µM 5.11E-5 18 9.07E-5
11 SGTC_1469 k284-2957 3.65 126.0 µM 7.59E-5 12 1.31E-4
12 SGTC_1395 3486-0190 3.59 4.3 µM 9.62E-5 24 1.64E-4
13 SGTC_1557 sumaresinolic acid 3.59 42.3 µM 9.83E-5 27 1.67E-4
14 SGTC_1097 itavastatin 3.56 7.2 µM 1.09E-4 azole & statin 31 1.83E-4
15 SGTC_1906 st060206 3.47 94.7 µM 1.55E-4 32 2.56E-4
16 SGTC_1140 3229-1848 3.46 3.0 µM 1.62E-4 17 2.66E-4
17 SGTC_211 3455-0679 3.41 143.5 µM 1.98E-4 36 3.21E-4
18 SGTC_731 3966-0296 3.30 387.0 µM 3.02E-4 36 4.76E-4
19 SGTC_886 0866-0150 3.28 153.0 µM 3.35E-4 45 5.24E-4
20 SGTC_3007 9078383 3.26 71.4 µM 3.52E-4 39 5.48E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.119 4.03E-12 YMR130W_p YMR130W_p hom Putative protein of unknown function; YMR130W is not an essential gene
0.103 2.17E-9 YGL067W NPY1 hom NADH diphosphatase (pyrophosphatase), hydrolyzes the pyrophosphate linkage in NADH and related nucleotides; localizes to peroxisomes; nudix hydrolase family member
0.088 3.20E-7 YJR094C IME1 hom Master regulator of meiosis that is active only during meiotic events, activates transcription of early meiotic genes through interaction with Ume6p, degraded by the 26S proteasome following phosphorylation by Ime2p
0.083 1.49E-6 YOR112W CEX1 hom Cytoplasmic component of the nuclear aminoacylation-dependent tRNA export pathway; interacts with nuclear pore component Nup116p; copurifies with tRNA export receptors Los1p and Msn5p, as well as eIF-1a and the RAN GTPase Gsp1p
0.082 1.83E-6 YPR105C COG4 het Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
0.081 2.80E-6 YIL091C UTP25 het Nucleolar protein required for 35S pre-RNA processing and 40S ribosomal subunit biogenesis
0.079 5.10E-6 YKL096C-B_p YKL096C-B_p hom Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
0.077 8.35E-6 YOR163W DDP1 hom Polyphosphate phosphatase; hydrolyzes diphosphorylated inositol polyphosphates and diadenosine polyphosphates; high specificity for diadenosine hexa- and pentaphosphates; contains endopolyphosphatase activity with a high affinity for polyphosphates, an activity also observed for its human DIPP homologs; possesses mRNA decapping activity; nudix hydrolase family member; protein abundance increases in response to DNA replication stress
0.075 1.24E-5 YPR035W GLN1 het Glutamine synthetase (GS); synthesizes glutamine from glutamate and ammonia; with Glt1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source and by amino acid limitation; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
0.074 1.57E-5 YLL034C RIX7 het Putative ATPase of the AAA family, required for export of pre-ribosomal large subunits from the nucleus; distributed between the nucleolus, nucleoplasm, and nuclear periphery depending on growth conditions
0.074 1.97E-5 YKR068C BET3 het Hydrophilic protein that acts in conjunction with SNARE proteins in targeting and fusion of ER to Golgi transport vesicles; component of the TRAPP (transport protein particle) complex
0.073 2.49E-5 YFL020C PAU5 hom Member of the seripauperin multigene family encoded mainly in subtelomeric regions; induced during alcoholic fermentation; induced by low temperature and also by anaerobic conditions; negatively regulated by oxygen and repressed by heme
0.072 2.86E-5 YGL097W SRM1 het Nucleotide exchange factor for Gsp1p, localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p
0.069 5.79E-5 YIL111W COX5B hom Subunit Vb of cytochrome c oxidase; cytochrome c oxidase is the terminal member of the mitochondrial inner membrane electron transport chain; Cox5Bp is predominantly expressed during anaerobic growth while its isoform Va (Cox5Ap) is expressed during aerobic growth; COX5B has a paralog, COX5A, that arose from the whole genome duplication
0.068 7.35E-5 YPR031W NTO1 hom Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3