YMR283C / RIT1

2'-O-ribosyl phosphate transferase, modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA

Zygosity: Homozygous strain
fixedexpanded
Profile for YMR283C / RIT1

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:2.92|P-value:8.93E-4 ID:SGTC_87|Compound:0100-0052|FD-Score:2.93|P-value:8.62E-4 ID:SGTC_306|Compound:1672-3436|FD-Score:2.93|P-value:8.76E-4 ID:SGTC_407|Compound:eburnamonine|FD-Score:-3.34|P-value:3.68E-4 ID:SGTC_497|Compound:capsazepine|FD-Score:-3.18|P-value:6.60E-4 ID:SGTC_679|Compound:0422-0091|FD-Score:2.96|P-value:7.77E-4 ID:SGTC_743|Compound:1275-0778|FD-Score:4.18|P-value:4.71E-6 ID:SGTC_884|Compound:0851-0618|FD-Score:3.26|P-value:2.56E-4 ID:SGTC_899|Compound:1083-0020|FD-Score:3.72|P-value:3.93E-5 ID:SGTC_1039|Compound:k821-0043|FD-Score:4.60|P-value:5.88E-7 ID:SGTC_1043|Compound:0916-0009|FD-Score:4.57|P-value:6.78E-7 ID:SGTC_1378|Compound:2093-0007|FD-Score:4.28|P-value:2.89E-6 ID:SGTC_1379|Compound:2144-0755|FD-Score:3.04|P-value:5.99E-4 ID:SGTC_1417|Compound:3966-0327|FD-Score:3.02|P-value:6.39E-4 ID:SGTC_1420|Compound:4'-methoxyflavone|FD-Score:2.93|P-value:8.85E-4 ID:SGTC_1434|Compound:4073-0095|FD-Score:3.24|P-value:2.76E-4 ID:SGTC_1520|Compound:flindersine|FD-Score:-3.41|P-value:2.81E-4 ID:SGTC_1636|Compound:st007707|FD-Score:6.90|P-value:2.02E-13 ID:SGTC_1660|Compound:st012960|FD-Score:-3.28|P-value:4.52E-4 ID:SGTC_1677|Compound:st019204|FD-Score:3.34|P-value:1.89E-4 ID:SGTC_1678|Compound:st019265|FD-Score:3.78|P-value:2.98E-5 ID:SGTC_1940|Compound:st075990|FD-Score:-3.96|P-value:2.85E-5 ID:SGTC_1972|Compound:st070201|FD-Score:2.97|P-value:7.51E-4 ID:SGTC_2140|Compound:5586273|FD-Score:2.98|P-value:7.27E-4 ID:SGTC_2146|Compound:5545642|FD-Score:3.17|P-value:3.58E-4 ID:SGTC_2190|Compound:6049624|FD-Score:3.55|P-value:7.90E-5 ID:SGTC_2311|Compound:7694451|FD-Score:7.90|P-value:5.57E-17 ID:SGTC_2323|Compound:6148992|FD-Score:2.94|P-value:8.57E-4 ID:SGTC_2329|Compound:6138490|FD-Score:3.51|P-value:9.61E-5 ID:SGTC_2387|Compound:9077560|FD-Score:3.37|P-value:1.69E-4 ID:SGTC_2391|Compound:6285546|FD-Score:-3.37|P-value:3.19E-4 ID:SGTC_2411|Compound:st077133|FD-Score:5.46|P-value:4.17E-9 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:4.88|P-value:1.27E-7 ID:SGTC_2522|Compound:biochanin a|FD-Score:3.49|P-value:1.02E-4 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:3.84|P-value:2.29E-5 ID:SGTC_2612|Compound:dictamnine|FD-Score:8.69|P-value:4.18E-20 ID:SGTC_2639|Compound:lactacystin|FD-Score:3.24|P-value:2.75E-4 ID:SGTC_2729|Compound:fluphenazine|FD-Score:3.17|P-value:3.68E-4 ID:SGTC_2730|Compound:imipramine|FD-Score:3.19|P-value:3.42E-4 ID:SGTC_2769|Compound:toremifene|FD-Score:-3.31|P-value:4.09E-4 ID:SGTC_2813|Compound:7971799|FD-Score:-3.47|P-value:2.19E-4 ID:SGTC_2934|Compound:9036370|FD-Score:3.27|P-value:2.46E-4 ID:SGTC_3044|Compound:9093193|FD-Score:-4.43|P-value:3.26E-6 ID:SGTC_3048|Compound:9093155|FD-Score:2.90|P-value:9.84E-4 ID:SGTC_3068|Compound:9116768|FD-Score:4.03|P-value:9.82E-6 ID:SGTC_3074|Compound:9116018|FD-Score:-3.31|P-value:4.02E-4 ID:SGTC_3151|Compound:9099821|FD-Score:2.98|P-value:7.34E-4 ID:SGTC_3263|Compound:9137018|FD-Score:-3.42|P-value:2.63E-4 ID:SGTC_3307|Compound:9126885|FD-Score:3.22|P-value:3.02E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:2.92|P-value:8.93E-4 ID:SGTC_87|Compound:0100-0052|FD-Score:2.93|P-value:8.62E-4 ID:SGTC_306|Compound:1672-3436|FD-Score:2.93|P-value:8.76E-4 ID:SGTC_407|Compound:eburnamonine|FD-Score:-3.34|P-value:3.68E-4 ID:SGTC_497|Compound:capsazepine|FD-Score:-3.18|P-value:6.60E-4 ID:SGTC_679|Compound:0422-0091|FD-Score:2.96|P-value:7.77E-4 ID:SGTC_743|Compound:1275-0778|FD-Score:4.18|P-value:4.71E-6 ID:SGTC_884|Compound:0851-0618|FD-Score:3.26|P-value:2.56E-4 ID:SGTC_899|Compound:1083-0020|FD-Score:3.72|P-value:3.93E-5 ID:SGTC_1039|Compound:k821-0043|FD-Score:4.60|P-value:5.88E-7 ID:SGTC_1043|Compound:0916-0009|FD-Score:4.57|P-value:6.78E-7 ID:SGTC_1378|Compound:2093-0007|FD-Score:4.28|P-value:2.89E-6 ID:SGTC_1379|Compound:2144-0755|FD-Score:3.04|P-value:5.99E-4 ID:SGTC_1417|Compound:3966-0327|FD-Score:3.02|P-value:6.39E-4 ID:SGTC_1420|Compound:4'-methoxyflavone|FD-Score:2.93|P-value:8.85E-4 ID:SGTC_1434|Compound:4073-0095|FD-Score:3.24|P-value:2.76E-4 ID:SGTC_1520|Compound:flindersine|FD-Score:-3.41|P-value:2.81E-4 ID:SGTC_1636|Compound:st007707|FD-Score:6.90|P-value:2.02E-13 ID:SGTC_1660|Compound:st012960|FD-Score:-3.28|P-value:4.52E-4 ID:SGTC_1677|Compound:st019204|FD-Score:3.34|P-value:1.89E-4 ID:SGTC_1678|Compound:st019265|FD-Score:3.78|P-value:2.98E-5 ID:SGTC_1940|Compound:st075990|FD-Score:-3.96|P-value:2.85E-5 ID:SGTC_1972|Compound:st070201|FD-Score:2.97|P-value:7.51E-4 ID:SGTC_2140|Compound:5586273|FD-Score:2.98|P-value:7.27E-4 ID:SGTC_2146|Compound:5545642|FD-Score:3.17|P-value:3.58E-4 ID:SGTC_2190|Compound:6049624|FD-Score:3.55|P-value:7.90E-5 ID:SGTC_2311|Compound:7694451|FD-Score:7.90|P-value:5.57E-17 ID:SGTC_2323|Compound:6148992|FD-Score:2.94|P-value:8.57E-4 ID:SGTC_2329|Compound:6138490|FD-Score:3.51|P-value:9.61E-5 ID:SGTC_2387|Compound:9077560|FD-Score:3.37|P-value:1.69E-4 ID:SGTC_2391|Compound:6285546|FD-Score:-3.37|P-value:3.19E-4 ID:SGTC_2411|Compound:st077133|FD-Score:5.46|P-value:4.17E-9 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:4.88|P-value:1.27E-7 ID:SGTC_2522|Compound:biochanin a|FD-Score:3.49|P-value:1.02E-4 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:3.84|P-value:2.29E-5 ID:SGTC_2612|Compound:dictamnine|FD-Score:8.69|P-value:4.18E-20 ID:SGTC_2639|Compound:lactacystin|FD-Score:3.24|P-value:2.75E-4 ID:SGTC_2729|Compound:fluphenazine|FD-Score:3.17|P-value:3.68E-4 ID:SGTC_2730|Compound:imipramine|FD-Score:3.19|P-value:3.42E-4 ID:SGTC_2769|Compound:toremifene|FD-Score:-3.31|P-value:4.09E-4 ID:SGTC_2813|Compound:7971799|FD-Score:-3.47|P-value:2.19E-4 ID:SGTC_2934|Compound:9036370|FD-Score:3.27|P-value:2.46E-4 ID:SGTC_3044|Compound:9093193|FD-Score:-4.43|P-value:3.26E-6 ID:SGTC_3048|Compound:9093155|FD-Score:2.90|P-value:9.84E-4 ID:SGTC_3068|Compound:9116768|FD-Score:4.03|P-value:9.82E-6 ID:SGTC_3074|Compound:9116018|FD-Score:-3.31|P-value:4.02E-4 ID:SGTC_3151|Compound:9099821|FD-Score:2.98|P-value:7.34E-4 ID:SGTC_3263|Compound:9137018|FD-Score:-3.42|P-value:2.63E-4 ID:SGTC_3307|Compound:9126885|FD-Score:3.22|P-value:3.02E-4

Top fitness defect scores for YMR283C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2612 dictamnine 8.69 15.5 µM 4.18E-20 RNA pol III & RNase P/MRP 5 1.84E-18
2 SGTC_2311 7694451 7.90 200.0 µM 5.57E-17 2 1.37E-15
3 SGTC_1636 st007707 6.90 68.9 µM 2.02E-13 15 2.55E-12
4 SGTC_2411 st077133 5.46 39.9 µM 4.17E-9 RNA pol III & RNase P/MRP 10 2.32E-8
5 SGTC_2499 avocadenofuran 4.88 51.5 µM 1.27E-7 17 5.31E-7
6 SGTC_1039 k821-0043 4.60 94.0 µM 5.88E-7 Golgi 13 2.15E-6
7 SGTC_1043 0916-0009 4.57 19.3 µM 6.78E-7 13 2.45E-6
8 SGTC_1378 2093-0007 4.28 380.0 nM 2.89E-6 15 9.23E-6
9 SGTC_743 1275-0778 4.18 158.0 µM 4.71E-6 excess fatty acid 31 1.44E-5
10 SGTC_3068 9116768 4.03 49.5 µM 9.82E-6 17 2.82E-5
11 SGTC_2573 2',4'-dihydroxychalcone 3.84 15.0 µM 2.29E-5 55 6.12E-5
12 SGTC_1678 st019265 3.78 80.2 µM 2.98E-5 60S ribosome export 25 7.79E-5
13 SGTC_899 1083-0020 3.72 478.0 µM 3.93E-5 28 1.00E-4
14 SGTC_2190 6049624 3.55 200.0 µM 7.90E-5 9 1.90E-4
15 SGTC_2329 6138490 3.51 200.0 µM 9.61E-5 48 2.27E-4
16 SGTC_2522 biochanin a 3.49 4.9 µM 1.02E-4 RNA pol III & RNase P/MRP 20 2.39E-4
17 SGTC_2387 9077560 3.37 195.1 µM 1.69E-4 34 3.79E-4
18 SGTC_1677 st019204 3.34 53.9 µM 1.89E-4 42 4.20E-4
19 SGTC_2934 9036370 3.27 36.9 µM 2.46E-4 61 5.34E-4
20 SGTC_884 0851-0618 3.26 334.0 µM 2.56E-4 40 5.53E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.226 5.09E-40 YNL221C POP1 het Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
0.197 1.45E-30 YDR211W GCD6 het Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress
0.184 7.14E-27 YLR291C GCD7 het Beta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
0.170 3.29E-23 YGR083C GCD2 het Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
0.152 6.67E-19 YOR116C RPO31 het RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
0.151 1.30E-18 YOR260W GCD1 het Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
0.128 1.25E-13 YPL178W CBC2 hom Small subunit of the heterodimeric cap binding complex that also contains Sto1p, component of the spliceosomal commitment complex; interacts with Npl3p, possibly to package mRNA for export from the nucleus; contains an RNA-binding motif
0.121 1.71E-12 YOR207C RET1 het Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
0.120 3.75E-12 YDL150W RPC53 het RNA polymerase III subunit C53
0.116 1.76E-11 YOR204W DED1 het ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility
0.114 4.31E-11 YGR247W CPD1 hom Cyclic nucleotide phosphodiesterase; hydrolyzes ADP-ribose 1'', 2''-cyclic phosphate to ADP-ribose 1''-phosphate; may have a role in tRNA splicing; no detectable phenotype is conferred by null mutation or by overexpression; protein abundance increases in response to DNA replication stress
0.112 7.35E-11 YAL017W PSK1 hom PAS domain-containing serine/threonine protein kinase; coordinately regulates protein synthesis and carbohydrate metabolism and storage in response to a unknown metabolite that reflects nutritional status; PSK1 has a paralog, PSK2, that arose from the whole genome duplication
0.109 2.15E-10 YMR253C_p YMR253C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene
0.109 2.17E-10 YBR257W POP4 het Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
0.104 1.62E-9 YJL064W_d YJL064W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL065C/DLS1