YMR285C / NGL2

Protein involved in 5.8S rRNA processing; Ccr4p-like RNase required for correct 3'-end formation of 5.8S rRNA at site E; similar to Ngl1p; NGL2 has a paralog, NGL3, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YMR285C / NGL2

Click on Significant Values for Screen Details ID:SGTC_69|Compound:0096-0274|FD-Score:3.99|P-value:5.48E-6 ID:SGTC_386|Compound:1509-0011|FD-Score:3.16|P-value:2.43E-4 ID:SGTC_504|Compound:lovastatin|FD-Score:2.90|P-value:6.87E-4 ID:SGTC_506|Compound:n9-isopropylolomoucine|FD-Score:2.84|P-value:8.49E-4 ID:SGTC_567|Compound:1080-0542|FD-Score:-3.13|P-value:3.05E-4 ID:SGTC_574|Compound:1315-0397|FD-Score:3.00|P-value:4.61E-4 ID:SGTC_662|Compound:k832-1615|FD-Score:2.81|P-value:9.49E-4 ID:SGTC_780|Compound:0109-0199|FD-Score:3.19|P-value:2.19E-4 ID:SGTC_806|Compound:3966-4455|FD-Score:2.93|P-value:6.19E-4 ID:SGTC_865|Compound:0488-0499|FD-Score:2.84|P-value:8.64E-4 ID:SGTC_884|Compound:0851-0618|FD-Score:3.24|P-value:1.74E-4 ID:SGTC_953|Compound:1188-0045|FD-Score:2.92|P-value:6.39E-4 ID:SGTC_1079|Compound:idarubicin|FD-Score:3.05|P-value:3.86E-4 ID:SGTC_1131|Compound:3454-3128|FD-Score:2.83|P-value:9.06E-4 ID:SGTC_1145|Compound:3546-0597|FD-Score:3.53|P-value:4.99E-5 ID:SGTC_1236|Compound:0330-0013|FD-Score:3.83|P-value:1.21E-5 ID:SGTC_1238|Compound:0333-0287|FD-Score:-2.83|P-value:9.79E-4 ID:SGTC_1522|Compound:st012842|FD-Score:-3.33|P-value:1.34E-4 ID:SGTC_1527|Compound:coumarin|FD-Score:3.87|P-value:1.01E-5 ID:SGTC_1731|Compound:st035964|FD-Score:-4.05|P-value:4.47E-6 ID:SGTC_1783|Compound:st039459|FD-Score:3.13|P-value:2.77E-4 ID:SGTC_1846|Compound:st056244|FD-Score:2.84|P-value:8.62E-4 ID:SGTC_2005|Compound:4024620|FD-Score:-2.87|P-value:8.42E-4 ID:SGTC_2037|Compound:5169046|FD-Score:-3.23|P-value:2.05E-4 ID:SGTC_2045|Compound:5106652|FD-Score:2.80|P-value:9.91E-4 ID:SGTC_2104|Compound:5389443|FD-Score:3.30|P-value:1.34E-4 ID:SGTC_2111|Compound:5486399|FD-Score:-3.11|P-value:3.36E-4 ID:SGTC_2294|Compound:7971431|FD-Score:2.88|P-value:7.29E-4 ID:SGTC_2363|Compound:9053361|FD-Score:3.45|P-value:7.01E-5 ID:SGTC_2395|Compound:5646831|FD-Score:3.46|P-value:6.86E-5 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:4.00|P-value:5.22E-6 ID:SGTC_2415|Compound:st077333|FD-Score:3.53|P-value:4.94E-5 ID:SGTC_2433|Compound:5607874|FD-Score:3.52|P-value:5.13E-5 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:2.88|P-value:7.30E-4 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:-2.84|P-value:9.53E-4 ID:SGTC_2628|Compound:lathosterol|FD-Score:4.55|P-value:2.65E-7 ID:SGTC_2640|Compound:pentamidine|FD-Score:3.34|P-value:1.15E-4 ID:SGTC_2685|Compound:mycophenolic acid|FD-Score:3.15|P-value:2.58E-4 ID:SGTC_2703|Compound:st077852|FD-Score:4.70|P-value:1.13E-7 ID:SGTC_2760|Compound:etidronate|FD-Score:3.91|P-value:8.00E-6 ID:SGTC_2784|Compound:7567673|FD-Score:2.82|P-value:9.41E-4 ID:SGTC_2811|Compound:7967822|FD-Score:4.43|P-value:5.14E-7 ID:SGTC_2830|Compound:7992007|FD-Score:-2.97|P-value:5.81E-4 ID:SGTC_2841|Compound:9004761|FD-Score:2.98|P-value:5.13E-4 ID:SGTC_3007|Compound:9078383|FD-Score:2.95|P-value:5.67E-4 ID:SGTC_3347|Compound:9188721|FD-Score:3.01|P-value:4.56E-4 ID:SGTC_69|Compound:0096-0274|FD-Score:3.99|P-value:5.48E-6 ID:SGTC_386|Compound:1509-0011|FD-Score:3.16|P-value:2.43E-4 ID:SGTC_504|Compound:lovastatin|FD-Score:2.90|P-value:6.87E-4 ID:SGTC_506|Compound:n9-isopropylolomoucine|FD-Score:2.84|P-value:8.49E-4 ID:SGTC_567|Compound:1080-0542|FD-Score:-3.13|P-value:3.05E-4 ID:SGTC_574|Compound:1315-0397|FD-Score:3.00|P-value:4.61E-4 ID:SGTC_662|Compound:k832-1615|FD-Score:2.81|P-value:9.49E-4 ID:SGTC_780|Compound:0109-0199|FD-Score:3.19|P-value:2.19E-4 ID:SGTC_806|Compound:3966-4455|FD-Score:2.93|P-value:6.19E-4 ID:SGTC_865|Compound:0488-0499|FD-Score:2.84|P-value:8.64E-4 ID:SGTC_884|Compound:0851-0618|FD-Score:3.24|P-value:1.74E-4 ID:SGTC_953|Compound:1188-0045|FD-Score:2.92|P-value:6.39E-4 ID:SGTC_1079|Compound:idarubicin|FD-Score:3.05|P-value:3.86E-4 ID:SGTC_1131|Compound:3454-3128|FD-Score:2.83|P-value:9.06E-4 ID:SGTC_1145|Compound:3546-0597|FD-Score:3.53|P-value:4.99E-5 ID:SGTC_1236|Compound:0330-0013|FD-Score:3.83|P-value:1.21E-5 ID:SGTC_1238|Compound:0333-0287|FD-Score:-2.83|P-value:9.79E-4 ID:SGTC_1522|Compound:st012842|FD-Score:-3.33|P-value:1.34E-4 ID:SGTC_1527|Compound:coumarin|FD-Score:3.87|P-value:1.01E-5 ID:SGTC_1731|Compound:st035964|FD-Score:-4.05|P-value:4.47E-6 ID:SGTC_1783|Compound:st039459|FD-Score:3.13|P-value:2.77E-4 ID:SGTC_1846|Compound:st056244|FD-Score:2.84|P-value:8.62E-4 ID:SGTC_2005|Compound:4024620|FD-Score:-2.87|P-value:8.42E-4 ID:SGTC_2037|Compound:5169046|FD-Score:-3.23|P-value:2.05E-4 ID:SGTC_2045|Compound:5106652|FD-Score:2.80|P-value:9.91E-4 ID:SGTC_2104|Compound:5389443|FD-Score:3.30|P-value:1.34E-4 ID:SGTC_2111|Compound:5486399|FD-Score:-3.11|P-value:3.36E-4 ID:SGTC_2294|Compound:7971431|FD-Score:2.88|P-value:7.29E-4 ID:SGTC_2363|Compound:9053361|FD-Score:3.45|P-value:7.01E-5 ID:SGTC_2395|Compound:5646831|FD-Score:3.46|P-value:6.86E-5 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:4.00|P-value:5.22E-6 ID:SGTC_2415|Compound:st077333|FD-Score:3.53|P-value:4.94E-5 ID:SGTC_2433|Compound:5607874|FD-Score:3.52|P-value:5.13E-5 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:2.88|P-value:7.30E-4 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:-2.84|P-value:9.53E-4 ID:SGTC_2628|Compound:lathosterol|FD-Score:4.55|P-value:2.65E-7 ID:SGTC_2640|Compound:pentamidine|FD-Score:3.34|P-value:1.15E-4 ID:SGTC_2685|Compound:mycophenolic acid|FD-Score:3.15|P-value:2.58E-4 ID:SGTC_2703|Compound:st077852|FD-Score:4.70|P-value:1.13E-7 ID:SGTC_2760|Compound:etidronate|FD-Score:3.91|P-value:8.00E-6 ID:SGTC_2784|Compound:7567673|FD-Score:2.82|P-value:9.41E-4 ID:SGTC_2811|Compound:7967822|FD-Score:4.43|P-value:5.14E-7 ID:SGTC_2830|Compound:7992007|FD-Score:-2.97|P-value:5.81E-4 ID:SGTC_2841|Compound:9004761|FD-Score:2.98|P-value:5.13E-4 ID:SGTC_3007|Compound:9078383|FD-Score:2.95|P-value:5.67E-4 ID:SGTC_3347|Compound:9188721|FD-Score:3.01|P-value:4.56E-4

Top fitness defect scores for YMR285C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2703 st077852 4.70 53.4 µM 1.13E-7 7 1.31E-6
2 SGTC_2628 lathosterol 4.55 100.0 µM 2.65E-7 16 2.66E-6
3 SGTC_2811 7967822 4.43 32.5 µM 5.14E-7 10 4.62E-6
4 SGTC_2410 aloe-emodin-8-o-glycoside 4.00 46.3 µM 5.22E-6 mitochondrial processes 29 3.18E-5
5 SGTC_69 0096-0274 3.99 206.7 µM 5.48E-6 mitochondrial processes 40 3.31E-5
6 SGTC_2760 etidronate 3.91 98.0 µM 8.00E-6 29 4.54E-5
7 SGTC_1527 coumarin 3.87 137.0 µM 1.01E-5 27 5.53E-5
8 SGTC_1236 0330-0013 3.83 258.0 µM 1.21E-5 12 6.43E-5
9 SGTC_2415 st077333 3.53 57.7 µM 4.94E-5 70 2.07E-4
10 SGTC_1145 3546-0597 3.53 10.2 µM 4.99E-5 mitochondrial processes 29 2.09E-4
11 SGTC_2433 5607874 3.52 27.8 µM 5.13E-5 30 2.14E-4
12 SGTC_2395 5646831 3.46 180.2 µM 6.86E-5 45 2.72E-4
13 SGTC_2363 9053361 3.45 176.0 µM 7.01E-5 Golgi 50 2.77E-4
14 SGTC_2640 pentamidine 3.34 10.4 µM 1.15E-4 mitochondrial processes 52 4.18E-4
15 SGTC_2104 5389443 3.30 200.0 µM 1.34E-4 41 4.76E-4
16 SGTC_884 0851-0618 3.24 334.0 µM 1.74E-4 42 5.94E-4
17 SGTC_780 0109-0199 3.19 192.0 µM 2.19E-4 50 7.19E-4
18 SGTC_386 1509-0011 3.16 3.1 µM 2.43E-4 184 7.82E-4
19 SGTC_2685 mycophenolic acid 3.15 18.9 µM 2.58E-4 RNA pol III & RNase P/MRP 25 8.23E-4
20 SGTC_1783 st039459 3.13 87.5 µM 2.77E-4 Golgi 51 8.74E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.175 1.96E-24 YLR449W FPR4 hom Peptidyl-prolyl cis-trans isomerase (PPIase) (proline isomerase) localized to the nucleus; catalyzes isomerization of proline residues in histones H3 and H4, which affects lysine methylation of those histones
0.122 1.41E-12 YKL194C MST1 hom Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro
0.113 5.65E-11 YML100W TSL1 hom Large subunit of trehalose 6-phosphate synthase/phosphatase complex; Tps1p-Tps2p complex converts uridine-5'-diphosphoglucose and glucose 6-phosphate to trehalose; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress; TSL1 has a paralog, TPS3, that arose from the whole genome duplication
0.111 1.04E-10 YPR020W ATP20 hom Subunit g of the mitochondrial F1F0 ATP synthase; reversibly phosphorylated on two residues; unphosphorylated form is required for dimerization of the ATP synthase complex
0.110 1.78E-10 YJL022W_d YJL022W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130
0.104 1.66E-9 YMR271C URA10 hom Minor orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in the de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA10 has a paralog, URA5, that arose from the whole genome duplication
0.101 4.77E-9 YDR460W TFB3 het Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit
0.101 4.91E-9 YNL181W_p YNL181W_p het Putative oxidoreductase; required for cell viability
0.097 1.63E-8 YMR173W DDR48 hom DNA damage-responsive protein; expression is increased in response to heat-shock stress or treatments that produce DNA lesions; contains multiple repeats of the amino acid sequence NNNDSYGS; protein abundance increases in response to DNA replication stress
0.090 1.81E-7 YER065C ICL1 hom Isocitrate lyase, catalyzes the formation of succinate and glyoxylate from isocitrate, a key reaction of the glyoxylate cycle; expression of ICL1 is induced by growth on ethanol and repressed by growth on glucose
0.089 2.39E-7 YHR116W COX23 hom Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs
0.089 2.74E-7 YBL030C PET9 het Major ADP/ATP carrier of the mitochondrial inner membrane; exchanges cytosolic ADP for mitochondrially synthesized ATP; also imports heme and ATP; phosphorylated; required for viability in many lab strains that carry a sal1 mutation; PET9 has a paralog, AAC3, that arose from the whole genome duplication
0.086 5.28E-7 YMR150C IMP1 hom Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p
0.086 5.89E-7 YOR208W PTP2 hom Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the nucleus
0.085 7.59E-7 YOL046C_d YOL046C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data;almost completely overlaps the verified gene PSK2/YOL045W