YMR291W / TDA1

Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; relocalizes from nucleus to cytoplasm upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YMR291W / TDA1

Click on Significant Values for Screen Details ID:SGTC_15|Compound:0266-0086|FD-Score:3.22|P-value:6.33E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-6.12|P-value:1.05E-9 ID:SGTC_421|Compound:streptovitacin|FD-Score:3.46|P-value:2.66E-4 ID:SGTC_425|Compound:3825-7017|FD-Score:-5.21|P-value:1.73E-7 ID:SGTC_554|Compound:4483-2211|FD-Score:3.23|P-value:6.09E-4 ID:SGTC_761|Compound:k216-0224|FD-Score:-3.22|P-value:8.78E-4 ID:SGTC_829|Compound:1635-0120|FD-Score:3.10|P-value:9.31E-4 ID:SGTC_839|Compound:0122-0028|FD-Score:3.76|P-value:8.47E-5 ID:SGTC_890|Compound:0929-0006|FD-Score:4.45|P-value:4.47E-6 ID:SGTC_924|Compound:2046-0044|FD-Score:-3.46|P-value:3.78E-4 ID:SGTC_931|Compound:2812-0076|FD-Score:-5.12|P-value:2.81E-7 ID:SGTC_1052|Compound:3253-3998|FD-Score:3.32|P-value:4.42E-4 ID:SGTC_1130|Compound:3454-2858|FD-Score:-3.33|P-value:5.99E-4 ID:SGTC_1323|Compound:1348-1351|FD-Score:-4.84|P-value:1.12E-6 ID:SGTC_1331|Compound:1472-0049|FD-Score:3.11|P-value:8.98E-4 ID:SGTC_1339|Compound:1486-1321|FD-Score:4.10|P-value:2.12E-5 ID:SGTC_1407|Compound:3909-7909|FD-Score:3.31|P-value:4.61E-4 ID:SGTC_1439|Compound:4092-1168|FD-Score:3.10|P-value:9.27E-4 ID:SGTC_1523|Compound:st012844|FD-Score:3.23|P-value:5.97E-4 ID:SGTC_1652|Compound:st011932|FD-Score:3.50|P-value:2.28E-4 ID:SGTC_1806|Compound:st050005|FD-Score:3.14|P-value:8.07E-4 ID:SGTC_2253|Compound:7938677|FD-Score:7.16|P-value:5.66E-13 ID:SGTC_2258|Compound:7960471|FD-Score:3.36|P-value:3.79E-4 ID:SGTC_2260|Compound:7964312|FD-Score:5.10|P-value:1.84E-7 ID:SGTC_2295|Compound:7971645|FD-Score:-3.47|P-value:3.62E-4 ID:SGTC_2325|Compound:6158266|FD-Score:-3.49|P-value:3.40E-4 ID:SGTC_2533|Compound:agelasine|FD-Score:4.62|P-value:1.98E-6 ID:SGTC_2568|Compound:diosmetin|FD-Score:3.77|P-value:8.10E-5 ID:SGTC_2579|Compound:2',5'-dihydroxy-4-methoxychalcone|FD-Score:3.38|P-value:3.55E-4 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:-3.65|P-value:1.91E-4 ID:SGTC_3295|Compound:9122362|FD-Score:-4.40|P-value:8.97E-6 ID:SGTC_3303|Compound:9125618|FD-Score:-3.64|P-value:1.97E-4 ID:SGTC_15|Compound:0266-0086|FD-Score:3.22|P-value:6.33E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-6.12|P-value:1.05E-9 ID:SGTC_421|Compound:streptovitacin|FD-Score:3.46|P-value:2.66E-4 ID:SGTC_425|Compound:3825-7017|FD-Score:-5.21|P-value:1.73E-7 ID:SGTC_554|Compound:4483-2211|FD-Score:3.23|P-value:6.09E-4 ID:SGTC_761|Compound:k216-0224|FD-Score:-3.22|P-value:8.78E-4 ID:SGTC_829|Compound:1635-0120|FD-Score:3.10|P-value:9.31E-4 ID:SGTC_839|Compound:0122-0028|FD-Score:3.76|P-value:8.47E-5 ID:SGTC_890|Compound:0929-0006|FD-Score:4.45|P-value:4.47E-6 ID:SGTC_924|Compound:2046-0044|FD-Score:-3.46|P-value:3.78E-4 ID:SGTC_931|Compound:2812-0076|FD-Score:-5.12|P-value:2.81E-7 ID:SGTC_1052|Compound:3253-3998|FD-Score:3.32|P-value:4.42E-4 ID:SGTC_1130|Compound:3454-2858|FD-Score:-3.33|P-value:5.99E-4 ID:SGTC_1323|Compound:1348-1351|FD-Score:-4.84|P-value:1.12E-6 ID:SGTC_1331|Compound:1472-0049|FD-Score:3.11|P-value:8.98E-4 ID:SGTC_1339|Compound:1486-1321|FD-Score:4.10|P-value:2.12E-5 ID:SGTC_1407|Compound:3909-7909|FD-Score:3.31|P-value:4.61E-4 ID:SGTC_1439|Compound:4092-1168|FD-Score:3.10|P-value:9.27E-4 ID:SGTC_1523|Compound:st012844|FD-Score:3.23|P-value:5.97E-4 ID:SGTC_1652|Compound:st011932|FD-Score:3.50|P-value:2.28E-4 ID:SGTC_1806|Compound:st050005|FD-Score:3.14|P-value:8.07E-4 ID:SGTC_2253|Compound:7938677|FD-Score:7.16|P-value:5.66E-13 ID:SGTC_2258|Compound:7960471|FD-Score:3.36|P-value:3.79E-4 ID:SGTC_2260|Compound:7964312|FD-Score:5.10|P-value:1.84E-7 ID:SGTC_2295|Compound:7971645|FD-Score:-3.47|P-value:3.62E-4 ID:SGTC_2325|Compound:6158266|FD-Score:-3.49|P-value:3.40E-4 ID:SGTC_2533|Compound:agelasine|FD-Score:4.62|P-value:1.98E-6 ID:SGTC_2568|Compound:diosmetin|FD-Score:3.77|P-value:8.10E-5 ID:SGTC_2579|Compound:2',5'-dihydroxy-4-methoxychalcone|FD-Score:3.38|P-value:3.55E-4 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:-3.65|P-value:1.91E-4 ID:SGTC_3295|Compound:9122362|FD-Score:-4.40|P-value:8.97E-6 ID:SGTC_3303|Compound:9125618|FD-Score:-3.64|P-value:1.97E-4

Top fitness defect scores for YMR291W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2253 7938677 7.16 135.8 µM 5.66E-13 2 4.15E-13
2 SGTC_2260 7964312 5.10 200.0 µM 1.84E-7 3 1.69E-7
3 SGTC_2533 agelasine 4.62 13.7 µM 1.98E-6 ERG2 31 1.88E-6
4 SGTC_890 0929-0006 4.45 47.2 µM 4.47E-6 12 4.31E-6
5 SGTC_1339 1486-1321 4.10 22.7 µM 2.12E-5 ERG2 18 2.09E-5
6 SGTC_2568 diosmetin 3.77 100.0 µM 8.10E-5 27 8.13E-5
7 SGTC_839 0122-0028 3.76 230.0 µM 8.47E-5 RSC complex & mRNA processing 46 8.50E-5
8 SGTC_1652 st011932 3.50 78.0 µM 2.28E-4 RSC complex & mRNA processing 26 2.31E-4
9 SGTC_421 streptovitacin 3.46 30.0 mM 2.66E-4 40 2.71E-4
10 SGTC_2579 2',5'-dihydroxy-4-methoxychalcone 3.38 13.2 µM 3.55E-4 46 3.63E-4
11 SGTC_2258 7960471 3.36 200.0 µM 3.79E-4 24 3.87E-4
12 SGTC_1052 3253-3998 3.32 198.0 µM 4.42E-4 44 4.53E-4
13 SGTC_1407 3909-7909 3.31 12.3 µM 4.61E-4 60S ribosome export 32 4.72E-4
14 SGTC_1523 st012844 3.23 44.7 µM 5.97E-4 60S ribosome export 52 6.14E-4
15 SGTC_554 4483-2211 3.23 187.0 µM 6.09E-4 39 6.26E-4
16 SGTC_15 0266-0086 3.22 27.1 µM 6.33E-4 endomembrane recycling 104 6.52E-4
17 SGTC_1806 st050005 3.14 60.3 µM 8.07E-4 48 8.33E-4
18 SGTC_1331 1472-0049 3.11 76.2 µM 8.98E-4 59 9.27E-4
19 SGTC_1439 4092-1168 3.10 46.0 µM 9.27E-4 30 9.57E-4
20 SGTC_829 1635-0120 3.10 117.0 µM 9.31E-4 DNA damage response 78 9.62E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.127 1.43E-13 YJL070C_p YJL070C_p hom Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; may regulate purine nucleotide homeostasis as overexpression in an AMD1 strain grown in adenine results in greatly reduced GDP and GTP intracellular levels; not an essential gene; YJL070C has a paralog, YBR284W, that arose from the whole genome duplication
0.119 5.19E-12 YPL026C SKS1 hom Putative serine/threonine protein kinase; involved in the adaptation to low concentrations of glucose independent of the SNF3 regulated pathway
0.086 6.89E-7 YDL083C RPS16B hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16B has a paralog, RPS16A, that arose from the whole genome duplication
0.085 9.22E-7 YIL078W THS1 het Threonyl-tRNA synthetase, essential cytoplasmic protein
0.084 1.12E-6 YOL005C RPB11 het RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit
0.082 2.09E-6 YER028C MIG3 hom Transcriptional regulator; partially nonfunctional in S288C strains but has a major role in catabolite repression and ethanol response in some other strains; involved in response to toxic agents; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genes; MIG3 has a paralog, MIG2, that arose from the whole genome duplication
0.080 3.31E-6 YOL008W COQ10 hom Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q6 to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes
0.080 3.71E-6 YHR065C RRP3 het Protein involved in rRNA processing; required for maturation of the 35S primary transcript of pre-rRNA and for cleavage leading to mature 18S rRNA; homologous to eIF-4a, which is a DEAD box RNA-dependent ATPase with helicase activity
0.079 5.16E-6 YOL046C_d YOL046C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data;almost completely overlaps the verified gene PSK2/YOL045W
0.074 1.65E-5 YPL040C ISM1 hom Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth
0.074 1.84E-5 YGL261C_p PAU11_p hom Putative protein of unknown function and member of the seripauperin multigene family encoded mainly in subtelomeric regions; mRNA expression appears to be regulated by SUT1 and UPC2
0.073 2.47E-5 YHR031C RRM3 hom DNA helicase involved in rDNA replication and Ty1 transposition; relieves replication fork pauses at telomeric regions; structurally and functionally related to Pif1p
0.072 3.13E-5 YML063W RPS1B hom Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1B has a paralog, RPS1A, that arose from the whole genome duplication
0.069 6.07E-5 YDL154W MSH5 hom Protein of the MutS family, forms a dimer with Msh4p that facilitates crossovers between homologs during meiosis; msh5-Y823H mutation confers tolerance to DNA alkylating agents; homologs present in C. elegans and humans
0.069 7.09E-5 YPR104C FHL1 het Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants