HIPHOP chemogenomics database
YMR300C / ADE4 
Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway
Zygosity: Homozygous strain
fixedexpanded
  Click on Significant Values for Screen Details |
Top fitness defect scores for YMR300C deletion by condition
Download Fitness data (tab-delimited text) (excel) |
Cofit Genes
Download Cofitness data (tab-delimited text) (excel)| Correlation | pval | ORF | Gene | Zygosity | Description |
|---|---|---|---|---|---|
| 0.416 | 1.37E-140 | YGR204W | ADE3 | hom | Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine |
| 0.300 | 1.19E-70 | YGL234W | ADE5,7 | hom | Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities |
| 0.279 | 4.93E-61 | YLR058C | SHM2 | hom | Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis |
| 0.274 | 6.93E-59 | YGR061C | ADE6 | hom | Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway |
| 0.229 | 6.67E-41 | YDR408C | ADE8 | hom | Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway |
| 0.138 | 1.14E-15 | YKR080W | MTD1 | hom | NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase, plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline |
| 0.129 | 7.63E-14 | YMR120C | ADE17 | hom | Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE17 has a paralog, ADE16, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine |
| 0.115 | 2.83E-11 | YJR046W | TAH11 | het | DNA replication licensing factor, required for pre-replication complex assembly |
| 0.105 | 9.28E-10 | YGR058W | PEF1 | hom | Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly |
| 0.102 | 2.87E-9 | YLR264W | RPS28B | hom | Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; RPS28B has a paralog, RPS28A, that arose from the whole genome duplication |
| 0.101 | 4.16E-9 | YGR239C | PEX21 | hom | Peroxin required for peroxisomal matrix protein targeting; acts on proteins containing the PTS2 targeting sequence; interacts with Pex7p; partially redundant with Pex18p; relative distribution to cytoplasmic foci increases upon DNA replication stress |
| 0.100 | 7.33E-9 | YGR273C_p | YGR273C_p | hom | Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene |
| 0.090 | 1.63E-7 | YLR140W_d | YLR140W_d | het | Dubious open reading frame unlikely to encode a functional protein; overlaps essential RRN5 gene which encodes a member of the UAF transcription factor involved in transcription of rDNA by RNA polymerase I |
| 0.085 | 7.48E-7 | YLL044W_d | YLL044W_d | hom | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLL044W and the overlapping gene RPL8B is reduced in the gcr1 null mutant |
| 0.085 | 8.77E-7 | YHR010W | RPL27A | hom | Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication |