YNL014W / HEF3

Translational elongation factor EF-3; member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells; HEF3 has a paralog, YEF3, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL014W / HEF3

Click on Significant Values for Screen Details ID:SGTC_62|Compound:0180-0423|FD-Score:3.25|P-value:9.54E-4 ID:SGTC_189|Compound:3970-0742|FD-Score:-3.48|P-value:4.21E-4 ID:SGTC_252|Compound:miconazole|FD-Score:-3.65|P-value:2.26E-4 ID:SGTC_430|Compound:0958-0115|FD-Score:-3.39|P-value:5.70E-4 ID:SGTC_634|Compound:1611-4317|FD-Score:-3.49|P-value:3.98E-4 ID:SGTC_647|Compound:0140-0236|FD-Score:-3.58|P-value:2.90E-4 ID:SGTC_816|Compound:0325-0417|FD-Score:5.29|P-value:2.07E-7 ID:SGTC_819|Compound:1013-0258|FD-Score:4.30|P-value:1.95E-5 ID:SGTC_820|Compound:1582-0060|FD-Score:-3.26|P-value:8.79E-4 ID:SGTC_823|Compound:0417-1665|FD-Score:-3.70|P-value:1.89E-4 ID:SGTC_862|Compound:0452-0056|FD-Score:3.44|P-value:5.11E-4 ID:SGTC_872|Compound:0588-0139|FD-Score:4.65|P-value:4.30E-6 ID:SGTC_964|Compound:1287-0107|FD-Score:3.30|P-value:7.94E-4 ID:SGTC_995|Compound:3931-2021|FD-Score:-3.38|P-value:5.86E-4 ID:SGTC_1004|Compound:4084-0029|FD-Score:3.23|P-value:0.00100 ID:SGTC_1243|Compound:0416-0003|FD-Score:-3.49|P-value:4.03E-4 ID:SGTC_1901|Compound:st060216|FD-Score:4.29|P-value:2.04E-5 ID:SGTC_1939|Compound:st074455|FD-Score:-4.25|P-value:2.21E-5 ID:SGTC_1955|Compound:wogonin|FD-Score:-4.41|P-value:1.17E-5 ID:SGTC_2112|Compound:5488546|FD-Score:3.31|P-value:7.72E-4 ID:SGTC_2162|Compound:5663267|FD-Score:-3.40|P-value:5.38E-4 ID:SGTC_2178|Compound:5812956|FD-Score:3.72|P-value:1.90E-4 ID:SGTC_2371|Compound:9071574|FD-Score:-3.65|P-value:2.26E-4 ID:SGTC_2424|Compound:5537928|FD-Score:3.97|P-value:7.28E-5 ID:SGTC_2484|Compound:5809818|FD-Score:-3.38|P-value:5.94E-4 ID:SGTC_2722|Compound:naftopidil|FD-Score:-3.44|P-value:4.77E-4 ID:SGTC_3199|Compound:9111318|FD-Score:6.63|P-value:1.15E-10 ID:SGTC_3200|Compound:9114385|FD-Score:5.21|P-value:3.18E-7 ID:SGTC_3216|Compound:9129716|FD-Score:3.59|P-value:2.95E-4 ID:SGTC_3348|Compound:9149464|FD-Score:4.86|P-value:1.65E-6 ID:SGTC_62|Compound:0180-0423|FD-Score:3.25|P-value:9.54E-4 ID:SGTC_189|Compound:3970-0742|FD-Score:-3.48|P-value:4.21E-4 ID:SGTC_252|Compound:miconazole|FD-Score:-3.65|P-value:2.26E-4 ID:SGTC_430|Compound:0958-0115|FD-Score:-3.39|P-value:5.70E-4 ID:SGTC_634|Compound:1611-4317|FD-Score:-3.49|P-value:3.98E-4 ID:SGTC_647|Compound:0140-0236|FD-Score:-3.58|P-value:2.90E-4 ID:SGTC_816|Compound:0325-0417|FD-Score:5.29|P-value:2.07E-7 ID:SGTC_819|Compound:1013-0258|FD-Score:4.30|P-value:1.95E-5 ID:SGTC_820|Compound:1582-0060|FD-Score:-3.26|P-value:8.79E-4 ID:SGTC_823|Compound:0417-1665|FD-Score:-3.70|P-value:1.89E-4 ID:SGTC_862|Compound:0452-0056|FD-Score:3.44|P-value:5.11E-4 ID:SGTC_872|Compound:0588-0139|FD-Score:4.65|P-value:4.30E-6 ID:SGTC_964|Compound:1287-0107|FD-Score:3.30|P-value:7.94E-4 ID:SGTC_995|Compound:3931-2021|FD-Score:-3.38|P-value:5.86E-4 ID:SGTC_1004|Compound:4084-0029|FD-Score:3.23|P-value:0.00100 ID:SGTC_1243|Compound:0416-0003|FD-Score:-3.49|P-value:4.03E-4 ID:SGTC_1901|Compound:st060216|FD-Score:4.29|P-value:2.04E-5 ID:SGTC_1939|Compound:st074455|FD-Score:-4.25|P-value:2.21E-5 ID:SGTC_1955|Compound:wogonin|FD-Score:-4.41|P-value:1.17E-5 ID:SGTC_2112|Compound:5488546|FD-Score:3.31|P-value:7.72E-4 ID:SGTC_2162|Compound:5663267|FD-Score:-3.40|P-value:5.38E-4 ID:SGTC_2178|Compound:5812956|FD-Score:3.72|P-value:1.90E-4 ID:SGTC_2371|Compound:9071574|FD-Score:-3.65|P-value:2.26E-4 ID:SGTC_2424|Compound:5537928|FD-Score:3.97|P-value:7.28E-5 ID:SGTC_2484|Compound:5809818|FD-Score:-3.38|P-value:5.94E-4 ID:SGTC_2722|Compound:naftopidil|FD-Score:-3.44|P-value:4.77E-4 ID:SGTC_3199|Compound:9111318|FD-Score:6.63|P-value:1.15E-10 ID:SGTC_3200|Compound:9114385|FD-Score:5.21|P-value:3.18E-7 ID:SGTC_3216|Compound:9129716|FD-Score:3.59|P-value:2.95E-4 ID:SGTC_3348|Compound:9149464|FD-Score:4.86|P-value:1.65E-6

Top fitness defect scores for YNL014W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3199 9111318 6.63 49.5 µM 1.15E-10 4 1.72E-11
2 SGTC_816 0325-0417 5.29 81.3 µM 2.07E-7 1 6.02E-8
3 SGTC_3200 9114385 5.21 49.5 µM 3.18E-7 11 9.64E-8
4 SGTC_3348 9149464 4.86 3.5 µM 1.65E-6 6 5.76E-7
5 SGTC_872 0588-0139 4.65 234.0 µM 4.30E-6 18 1.64E-6
6 SGTC_819 1013-0258 4.30 27.7 µM 1.95E-5 31 8.47E-6
7 SGTC_1901 st060216 4.29 58.6 µM 2.04E-5 17 8.89E-6
8 SGTC_2424 5537928 3.97 59.2 µM 7.28E-5 39 3.55E-5
9 SGTC_2178 5812956 3.72 200.0 µM 1.90E-4 21 1.01E-4
10 SGTC_3216 9129716 3.59 49.5 µM 2.95E-4 RPP1 & pyrimidine depletion 34 1.62E-4
11 SGTC_862 0452-0056 3.44 10.2 µM 5.11E-4 azole & statin 45 2.95E-4
12 SGTC_2112 5488546 3.31 200.0 µM 7.72E-4 TSC3-RPN4 38 4.61E-4
13 SGTC_964 1287-0107 3.30 19.4 µM 7.94E-4 cell wall 75 4.75E-4
14 SGTC_62 0180-0423 3.25 3.2 µM 9.54E-4 60S ribosome export 43 5.80E-4
15 SGTC_1004 4084-0029 3.23 199.0 µM 0.00100 48 6.10E-4
16 SGTC_668 1682-0499 3.22 62.6 µM 0.00104 57 6.38E-4
17 SGTC_77 0828-0286 3.16 12.5 µM 0.00127 TSC3-RPN4 80 7.92E-4
18 SGTC_450 aminopyrene 3.12 156.0 nM 0.00143 74 9.01E-4
19 SGTC_2538 3',7'-epoxycaryophyllan-6-one 3.07 100.0 µM 0.00169 55 0.00108
20 SGTC_3205 9113483 3.06 49.5 µM 0.00171 78 0.00110

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.107 5.83E-10 YOR154W_p SLP1_p hom Glycosylated integral ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Emp65p; member of the SUN-like family of proteins; genetic interactions suggest a role in folding of ER membrane proteins; required for nuclear envelope localization of Mps3p
0.105 8.82E-10 YJL174W KRE9 het Glycoprotein involved in cell wall beta-glucan assembly; null mutation leads to severe growth defects, aberrant multibudded morphology, and mating defects
0.105 9.18E-10 YLR208W SEC13 het Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
0.102 3.88E-9 YHR172W SPC97 het Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
0.092 9.85E-8 YKR096W ESL2 hom Protein of unknown function that interacts with Pex14p; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; predicted to contain a PINc domain
0.088 3.29E-7 YOR378W_p YOR378W_p hom Putative paralog of ATR1, but not required for boron tolerance; member of the DHA2 family of drug:H+ antiporters; YOR378W is not an essential gene
0.079 4.69E-6 YPL152W RRD2 hom Peptidyl-prolyl cis/trans-isomerase; also activates the phosphotyrosyl phosphatase activity of protein phosphatase 2A (PP2A); regulates G1 phase progression, the osmoresponse, microtubule dynamics; subunit of the Tap42p-Pph21p-Rrd2p complex; protein abundance increases in response to DNA replication stress
0.076 9.48E-6 YPL236C ENV7 hom Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16)
0.073 2.43E-5 YER185W PUG1 hom Plasma membrane protein with roles in the uptake of protoprophyrin IX and the efflux of heme; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of proteins
0.073 2.67E-5 YDR162C NBP2 hom Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM)
0.072 3.25E-5 YCL047C POF1 hom ATPase involved in the protein quality control and filamentation pathways; interacts physically with Kss1p and suppresses the filamentation defect of a kss1 deletion; also interacts with Ubc7p
0.070 4.57E-5 YPL167C REV3 hom Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair
0.069 6.97E-5 YGL224C SDT1 hom Pyrimidine nucleotidase; overexpression suppresses the 6-AU sensitivity of transcription elongation factor S-II, as well as resistance to other pyrimidine derivatives
0.067 9.36E-5 YBR158W AMN1 hom Protein required for daughter cell separation, multiple mitotic checkpoints, and chromosome stability; contains 12 degenerate leucine-rich repeat motifs; expression is induced by the Mitotic Exit Network (MEN)
0.067 1.11E-4 YBR005W RCR1 hom Protein of the ER membrane involved in cell wall chitin deposition; may function in the endosomal-vacuolar trafficking pathway, helping determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR1 has a paralog, RCR2, that arose from the whole genome duplication