Protein of unknown function; contains WD-40 domains; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YNL035C is not an essential gene; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
Profile for YNL035C / YNL035C

Click on Significant Values for Screen Details

Top fitness defect scores for YNL035C deletion by condition

RankScreen IDConditionFD ScoreConc
1 SGTC_2933 9026794 6.74 32.5 µM 4.38E-13 2 8.15E-12
2 SGTC_3326 9140064 5.35 16.4 µM 6.60E-9 8 4.50E-8
3 SGTC_836 0330-0104 4.30 1.4 µM 2.25E-6 8 8.36E-6
4 SGTC_127 0180-0333 4.20 59.1 µM 3.76E-6 mitochondrial response to ROS 34 1.32E-5
5 SGTC_369 0957-0393 4.14 4.5 µM 5.20E-6 cell wall signaling 19 1.77E-5
6 SGTC_1261 0712-0010 4.09 12.7 µM 6.57E-6 RNA processing & uracil transport 19 2.18E-5
7 SGTC_753 1322-0200 4.05 289.0 µM 7.92E-6 16 2.57E-5
8 SGTC_1894 3',6'-dihydroxyflavone 3.96 78.7 µM 1.22E-5 12 3.77E-5
9 SGTC_272 doxorubicin 3.92 8.1 µM 1.43E-5 anthracycline transcription coupled DNA repair 20 4.36E-5
10 SGTC_3133 9127736 3.90 49.5 µM 1.57E-5 18 4.75E-5
11 SGTC_1166 k206-0078 3.67 225.0 µM 4.39E-5 27 1.19E-4
12 SGTC_387 norethindrone acetate 3.66 66.7 µM 4.67E-5 23 1.26E-4
13 SGTC_1414 3917-0015 3.58 5.4 µM 6.50E-5 18 1.69E-4
14 SGTC_390 dec-rvkr-cmk 3.58 16.7 µM 6.52E-5 calcium & mitochondrial duress 39 1.70E-4
15 SGTC_2634 4'-methoxyflavone 3.56 100.0 µM 7.23E-5 47 1.86E-4
16 SGTC_1637 st007924 3.52 17.4 µM 8.53E-5 42 2.16E-4
17 SGTC_865 0488-0499 3.48 301.0 µM 1.01E-4 30 2.52E-4
18 SGTC_1841 st054148 3.43 70.0 µM 1.24E-4 19 3.02E-4
19 SGTC_2342 7990637 3.43 118.9 µM 1.25E-4 RNA processing & uracil transport 59 3.03E-4
20 SGTC_2069 5228469 3.39 200.0 µM 1.45E-4 31 3.47E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.128 8.96E-14 YOR329C SCD5 het Protein required for normal actin organization and endocytosis; targeting subunit for protein phosphatase type 1; undergoes Crm1p-dependent nuclear-cytoplasmic shuttling; multicopy suppressor of clathrin deficiency
0.113 4.95E-11 YJL049W_p YJL049W_p hom Putative protein of unknown function; YJL049W is a non-essential gene
0.104 1.37E-9 YIL143C SSL2 het Component of RNA polymerase transcription factor TFIIH holoenzyme; has DNA-dependent ATPase/helicase activity and is required, with Rad3p, for unwinding promoter DNA; interacts functionally with TFIIB and has roles in transcription start site selection and in gene looping to juxtapose initiation and termination regions; involved in DNA repair; homolog of human ERCC3
0.102 2.87E-9 YKL097C_d YKL097C_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species
0.088 3.21E-7 YPL113C_p YPL113C_p hom Glyoxylate reductase; acts on glyoxylate and hydroxypyruvate substrates; YPL113C is not an essential gene
0.087 4.69E-7 YPL012W RRP12 het Protein required for export of the ribosomal subunits; associates with the RNA components of the pre-ribosomes; has a role in nuclear import in association with Pse1p; contains HEAT-repeats
0.085 7.72E-7 YLL055W YCT1 hom High-affinity cysteine-specific transporter with similarity to the Dal5p family of transporters; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YCT1 is not an essential gene
0.084 9.80E-7 YKR041W YKR041W hom Protein of unknown function; localizes to the mitotic spindle; overexpression of YKR041W affects endocytic protein trafficking
0.083 1.62E-6 YNL339C YRF1-6 hom Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p
0.081 2.37E-6 YPR037C ERV2 hom Flavin-linked sulfhydryl oxidase localized to the endoplasmic reticulum lumen, involved in disulfide bond formation within the ER
0.081 2.42E-6 YFR035C_p YFR035C_p hom Putative protein of unknown function, deletion mutant exhibits synthetic phenotype with alpha-synuclein
0.077 7.68E-6 YBR217W ATG12 hom Conserved ubiquitin-like modifier involved in autophagy and the Cvt pathway; conjugated to Atg5p to form a complex involved in Atg8p lipidation; Atg12p-Atg5p also forms a complex with Atg16p that is required for autophagosome formation
0.076 9.24E-6 YLR284C ECI1 hom Peroxisomal delta3,delta2-enoyl-CoA isomerase, hexameric protein that converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA, essential for the beta-oxidation of unsaturated fatty acids, oleate-induced
0.076 1.11E-5 YOR353C SOG2 het Key component of the RAM signaling network, required for proper cell morphogenesis and cell separation after mitosis
0.075 1.20E-5 YCR107W AAD3 hom Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role