YNL074C / MLF3

Serine-rich protein of unknown function, predicted to be palmitoylated; overproduction suppresses the growth inhibition caused by exposure to the immunosuppressant leflunomide

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL074C / MLF3

Click on Significant Values for Screen Details ID:SGTC_534|Compound:k292-0788|FD-Score:3.14|P-value:3.15E-4 ID:SGTC_626|Compound:0214-0009|FD-Score:3.14|P-value:3.17E-4 ID:SGTC_1348|Compound:1493-0373|FD-Score:3.28|P-value:1.75E-4 ID:SGTC_1585|Compound:2',4'-dihydroxychalcone|FD-Score:3.59|P-value:4.58E-5 ID:SGTC_1699|Compound:st031318|FD-Score:3.19|P-value:2.49E-4 ID:SGTC_1737|Compound:st035557|FD-Score:-4.32|P-value:9.51E-7 ID:SGTC_1745|Compound:st081495|FD-Score:4.61|P-value:2.42E-7 ID:SGTC_2014|Compound:4013930|FD-Score:3.73|P-value:2.35E-5 ID:SGTC_2029|Compound:5175081|FD-Score:-3.28|P-value:1.49E-4 ID:SGTC_2037|Compound:5169046|FD-Score:3.37|P-value:1.18E-4 ID:SGTC_2333|Compound:9017922|FD-Score:2.87|P-value:8.77E-4 ID:SGTC_2359|Compound:9039622|FD-Score:3.19|P-value:2.56E-4 ID:SGTC_2394|Compound:5221648|FD-Score:2.84|P-value:9.89E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:8.23|P-value:1.02E-19 ID:SGTC_2629|Compound:bisabolol|FD-Score:2.95|P-value:6.68E-4 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:-2.91|P-value:6.47E-4 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:3.75|P-value:2.19E-5 ID:SGTC_2690|Compound:usnic acid|FD-Score:3.95|P-value:8.35E-6 ID:SGTC_2693|Compound:st077581|FD-Score:3.16|P-value:2.90E-4 ID:SGTC_2817|Compound:7995342|FD-Score:-3.17|P-value:2.28E-4 ID:SGTC_2834|Compound:9001755|FD-Score:-3.69|P-value:2.29E-5 ID:SGTC_2835|Compound:9006588|FD-Score:-2.97|P-value:5.08E-4 ID:SGTC_2934|Compound:9036370|FD-Score:3.31|P-value:1.54E-4 ID:SGTC_2989|Compound:9073649|FD-Score:3.44|P-value:8.76E-5 ID:SGTC_2992|Compound:9074591|FD-Score:-3.08|P-value:3.31E-4 ID:SGTC_534|Compound:k292-0788|FD-Score:3.14|P-value:3.15E-4 ID:SGTC_626|Compound:0214-0009|FD-Score:3.14|P-value:3.17E-4 ID:SGTC_1348|Compound:1493-0373|FD-Score:3.28|P-value:1.75E-4 ID:SGTC_1585|Compound:2',4'-dihydroxychalcone|FD-Score:3.59|P-value:4.58E-5 ID:SGTC_1699|Compound:st031318|FD-Score:3.19|P-value:2.49E-4 ID:SGTC_1737|Compound:st035557|FD-Score:-4.32|P-value:9.51E-7 ID:SGTC_1745|Compound:st081495|FD-Score:4.61|P-value:2.42E-7 ID:SGTC_2014|Compound:4013930|FD-Score:3.73|P-value:2.35E-5 ID:SGTC_2029|Compound:5175081|FD-Score:-3.28|P-value:1.49E-4 ID:SGTC_2037|Compound:5169046|FD-Score:3.37|P-value:1.18E-4 ID:SGTC_2333|Compound:9017922|FD-Score:2.87|P-value:8.77E-4 ID:SGTC_2359|Compound:9039622|FD-Score:3.19|P-value:2.56E-4 ID:SGTC_2394|Compound:5221648|FD-Score:2.84|P-value:9.89E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:8.23|P-value:1.02E-19 ID:SGTC_2629|Compound:bisabolol|FD-Score:2.95|P-value:6.68E-4 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:-2.91|P-value:6.47E-4 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:3.75|P-value:2.19E-5 ID:SGTC_2690|Compound:usnic acid|FD-Score:3.95|P-value:8.35E-6 ID:SGTC_2693|Compound:st077581|FD-Score:3.16|P-value:2.90E-4 ID:SGTC_2817|Compound:7995342|FD-Score:-3.17|P-value:2.28E-4 ID:SGTC_2834|Compound:9001755|FD-Score:-3.69|P-value:2.29E-5 ID:SGTC_2835|Compound:9006588|FD-Score:-2.97|P-value:5.08E-4 ID:SGTC_2934|Compound:9036370|FD-Score:3.31|P-value:1.54E-4 ID:SGTC_2989|Compound:9073649|FD-Score:3.44|P-value:8.76E-5 ID:SGTC_2992|Compound:9074591|FD-Score:-3.08|P-value:3.31E-4

Top fitness defect scores for YNL074C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2582 agaric acid 8.23 100.0 µM 1.02E-19 redox potentiating 98 9.03E-17
2 SGTC_1745 st081495 4.61 74.0 µM 2.42E-7 RNA processing & uracil transport 55 2.03E-6
3 SGTC_2690 usnic acid 3.95 100.0 µM 8.35E-6 27 3.99E-5
4 SGTC_2633 5,7-dihydroxyisoflavone 3.75 100.0 µM 2.19E-5 36 9.00E-5
5 SGTC_2014 4013930 3.73 200.0 µM 2.35E-5 42 9.54E-5
6 SGTC_1585 2',4'-dihydroxychalcone 3.59 10.4 µM 4.58E-5 47 1.67E-4
7 SGTC_2989 9073649 3.44 71.4 µM 8.76E-5 61 2.89E-4
8 SGTC_2037 5169046 3.37 200.0 µM 1.18E-4 60S ribosome export 24 3.71E-4
9 SGTC_2934 9036370 3.31 36.9 µM 1.54E-4 56 4.67E-4
10 SGTC_1348 1493-0373 3.28 93.8 µM 1.75E-4 32 5.18E-4
11 SGTC_1699 st031318 3.19 29.1 µM 2.49E-4 43 6.99E-4
12 SGTC_2359 9039622 3.19 200.0 µM 2.56E-4 endomembrane recycling 55 7.17E-4
13 SGTC_2693 st077581 3.16 40.7 µM 2.90E-4 79 7.95E-4
14 SGTC_534 k292-0788 3.14 63.4 µM 3.15E-4 mitochondrial processes 58 8.54E-4
15 SGTC_626 0214-0009 3.14 33.0 µM 3.17E-4 copper-dependent oxidative stress 54 8.57E-4
16 SGTC_2629 bisabolol 2.95 100.0 µM 6.68E-4 71 0.00161
17 SGTC_2333 9017922 2.87 200.0 µM 8.77E-4 80 0.00203
18 SGTC_2394 5221648 2.84 200.0 µM 9.89E-4 88 0.00225
19 SGTC_2042 p-Dimethylaminoacetophenone 2.82 200.0 µM 0.00108 75 0.00242
20 SGTC_2645 abamectin 2.78 100.0 µM 0.00123 heme requiring 82 0.00271

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.349 7.75E-97 YML103C NUP188 hom Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC organization and nucleocytoplasmic transport; homologous to human NUP188
0.314 1.52E-77 YNL106C INP52 hom Polyphosphatidylinositol phosphatase, dephosphorylates a number of phosphatidylinositols (PIs) to PI; involved in endocytosis; hyperosmotic stress causes translocation to actin patches; synaptojanin-like protein with a Sac1 domain
0.295 1.61E-68 YMR109W MYO5 hom One of two type I myosins; contains proline-rich tail homology 2 (TH2) and SH3 domains; MYO5 deletion has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization
0.290 5.18E-66 YNL027W CRZ1 hom Transcription factor, activates transcription of stress response genes; nuclear localization is positively regulated by calcineurin-mediated dephosphorylation; rapidly localizes to the nucleus under blue light stress
0.280 1.11E-61 YOR049C RSB1 hom Suppressor of sphingoid long chain base (LCB) sensitivity of an LCB-lyase mutation; putative integral membrane transporter or flippase that may transport LCBs from the cytoplasmic side toward the extracytoplasmic side of the membrane
0.276 8.10E-60 YLR206W ENT2 hom Epsin-like protein required for endocytosis and actin patch assembly and functionally redundant with Ent1p; contains clathrin-binding motif at C-terminus
0.274 1.02E-58 YEL007W MIT1 hom Transcriptional regulator of pseudohyphal growth; protein with sequence similarity to S. pombe gti1+ (gluconate transport inducer 1) and C. albicans Wor1
0.269 1.44E-56 YML052W SUR7 hom Plasma membrane protein that localizes to furrow-like invaginations (MCC patches); component of eisosomes; associated with endocytosis, along with Pil1p and Lsp1p; sporulation and plasma membrane sphingolipid content are altered in mutants
0.267 8.94E-56 YPL232W SSO1 hom Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p
0.257 1.43E-51 YML102W CAC2 hom Subunit of chromatin assembly factor I (CAF-1), with Rlf2p and Msi1p; chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription
0.246 2.25E-47 YOR050C_d YOR050C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutation is viable
0.242 4.00E-46 YNL105W_d RRT16_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene INP52; identified in a screen for mutants with decreased levels of rDNA transcription
0.235 3.18E-43 YIL129C TAO3 het Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with protein kinase Cbk1p and also with Kic1p
0.226 3.43E-40 YGL178W MPT5 hom mRNA-binding protein of the PUF family; binds to the 3' UTR of specific mRNAs, including those involved in mating type switching, cell wall integrity, chronological lifespan, chromatin modification, and spindle pole body architecture; recruits the CCR4-NOT deadenylase complex to mRNAs along with Dhh1p and Dcp1p to promote deadenylation, decapping, and decay; also interacts with the Caf20p translational initiation repressor, affecting its mRNA target specificity
0.217 4.50E-37 YOL060C MAM3 hom Protein required for normal mitochondrial morphology, has similarity to hemolysins