YNL078W / NIS1

Protein localized in the bud neck at G2/M phase; physically interacts with septins; possibly involved in a mitotic signaling network

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL078W / NIS1

Click on Significant Values for Screen Details ID:SGTC_40|Compound:0327-0103|FD-Score:3.06|P-value:2.27E-4 ID:SGTC_292|Compound:k038-0015|FD-Score:-3.22|P-value:1.50E-4 ID:SGTC_401|Compound:neomycin|FD-Score:-4.65|P-value:7.85E-8 ID:SGTC_412|Compound:gemfibrozil|FD-Score:-3.55|P-value:3.32E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.26|P-value:9.46E-5 ID:SGTC_486|Compound:niguldipine|FD-Score:3.68|P-value:1.30E-5 ID:SGTC_620|Compound:0109-0052|FD-Score:2.69|P-value:9.97E-4 ID:SGTC_621|Compound:3584-0052|FD-Score:3.65|P-value:1.50E-5 ID:SGTC_633|Compound:0437-0093|FD-Score:-3.55|P-value:3.28E-5 ID:SGTC_871|Compound:0574-0004|FD-Score:-2.88|P-value:6.21E-4 ID:SGTC_875|Compound:0709-0210|FD-Score:2.73|P-value:8.68E-4 ID:SGTC_892|Compound:0929-0076|FD-Score:-2.80|P-value:8.38E-4 ID:SGTC_900|Compound:1112-0045|FD-Score:-3.38|P-value:7.20E-5 ID:SGTC_1040|Compound:k898-0338|FD-Score:2.71|P-value:9.34E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-5.01|P-value:7.75E-9 ID:SGTC_1143|Compound:3454-2408|FD-Score:2.95|P-value:3.61E-4 ID:SGTC_1180|Compound:1193-0155|FD-Score:3.42|P-value:4.62E-5 ID:SGTC_1200|Compound:1643-0193|FD-Score:3.75|P-value:9.02E-6 ID:SGTC_1215|Compound:3852-0577|FD-Score:4.07|P-value:1.63E-6 ID:SGTC_1260|Compound:0700-1617|FD-Score:-2.97|P-value:4.22E-4 ID:SGTC_1613|Compound:st002381|FD-Score:-2.88|P-value:6.10E-4 ID:SGTC_1737|Compound:st035557|FD-Score:-3.41|P-value:6.21E-5 ID:SGTC_1858|Compound:4-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione|FD-Score:3.11|P-value:1.85E-4 ID:SGTC_1902|Compound:st060212|FD-Score:-3.16|P-value:1.88E-4 ID:SGTC_2087|Compound:5276107|FD-Score:-2.86|P-value:6.72E-4 ID:SGTC_2096|Compound:5468907|FD-Score:3.08|P-value:2.14E-4 ID:SGTC_2102|Compound:5377816|FD-Score:3.78|P-value:7.89E-6 ID:SGTC_2103|Compound:5378138|FD-Score:2.86|P-value:5.19E-4 ID:SGTC_2125|Compound:5246583|FD-Score:4.82|P-value:1.93E-8 ID:SGTC_2137|Compound:5261020|FD-Score:-2.83|P-value:7.48E-4 ID:SGTC_2281|Compound:7943571|FD-Score:3.09|P-value:1.99E-4 ID:SGTC_2470|Compound:5664817|FD-Score:-3.73|P-value:1.37E-5 ID:SGTC_2487|Compound:5918373|FD-Score:3.37|P-value:5.81E-5 ID:SGTC_2508|Compound:celastrol|FD-Score:-2.84|P-value:7.28E-4 ID:SGTC_2589|Compound:lobaric acid|FD-Score:-3.65|P-value:2.04E-5 ID:SGTC_2660|Compound:2',4'-dihydroxychalcone|FD-Score:2.99|P-value:3.03E-4 ID:SGTC_2750|Compound:bromebric acid|FD-Score:3.08|P-value:2.11E-4 ID:SGTC_2757|Compound:clomiphene|FD-Score:5.27|P-value:9.11E-10 ID:SGTC_2759|Compound:disulfiram|FD-Score:-3.52|P-value:3.77E-5 ID:SGTC_2943|Compound:9068529|FD-Score:-3.46|P-value:4.97E-5 ID:SGTC_2969|Compound:9091408|FD-Score:-3.54|P-value:3.37E-5 ID:SGTC_2971|Compound:9092186|FD-Score:-3.16|P-value:1.90E-4 ID:SGTC_3015|Compound:9080845|FD-Score:3.34|P-value:6.54E-5 ID:SGTC_3291|Compound:9121177|FD-Score:-2.78|P-value:9.15E-4 ID:SGTC_3351|Compound:9150312|FD-Score:-3.42|P-value:5.85E-5 ID:SGTC_3355|Compound:9151058|FD-Score:-2.92|P-value:5.20E-4 ID:SGTC_40|Compound:0327-0103|FD-Score:3.06|P-value:2.27E-4 ID:SGTC_292|Compound:k038-0015|FD-Score:-3.22|P-value:1.50E-4 ID:SGTC_401|Compound:neomycin|FD-Score:-4.65|P-value:7.85E-8 ID:SGTC_412|Compound:gemfibrozil|FD-Score:-3.55|P-value:3.32E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.26|P-value:9.46E-5 ID:SGTC_486|Compound:niguldipine|FD-Score:3.68|P-value:1.30E-5 ID:SGTC_620|Compound:0109-0052|FD-Score:2.69|P-value:9.97E-4 ID:SGTC_621|Compound:3584-0052|FD-Score:3.65|P-value:1.50E-5 ID:SGTC_633|Compound:0437-0093|FD-Score:-3.55|P-value:3.28E-5 ID:SGTC_871|Compound:0574-0004|FD-Score:-2.88|P-value:6.21E-4 ID:SGTC_875|Compound:0709-0210|FD-Score:2.73|P-value:8.68E-4 ID:SGTC_892|Compound:0929-0076|FD-Score:-2.80|P-value:8.38E-4 ID:SGTC_900|Compound:1112-0045|FD-Score:-3.38|P-value:7.20E-5 ID:SGTC_1040|Compound:k898-0338|FD-Score:2.71|P-value:9.34E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-5.01|P-value:7.75E-9 ID:SGTC_1143|Compound:3454-2408|FD-Score:2.95|P-value:3.61E-4 ID:SGTC_1180|Compound:1193-0155|FD-Score:3.42|P-value:4.62E-5 ID:SGTC_1200|Compound:1643-0193|FD-Score:3.75|P-value:9.02E-6 ID:SGTC_1215|Compound:3852-0577|FD-Score:4.07|P-value:1.63E-6 ID:SGTC_1260|Compound:0700-1617|FD-Score:-2.97|P-value:4.22E-4 ID:SGTC_1613|Compound:st002381|FD-Score:-2.88|P-value:6.10E-4 ID:SGTC_1737|Compound:st035557|FD-Score:-3.41|P-value:6.21E-5 ID:SGTC_1858|Compound:4-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione|FD-Score:3.11|P-value:1.85E-4 ID:SGTC_1902|Compound:st060212|FD-Score:-3.16|P-value:1.88E-4 ID:SGTC_2087|Compound:5276107|FD-Score:-2.86|P-value:6.72E-4 ID:SGTC_2096|Compound:5468907|FD-Score:3.08|P-value:2.14E-4 ID:SGTC_2102|Compound:5377816|FD-Score:3.78|P-value:7.89E-6 ID:SGTC_2103|Compound:5378138|FD-Score:2.86|P-value:5.19E-4 ID:SGTC_2125|Compound:5246583|FD-Score:4.82|P-value:1.93E-8 ID:SGTC_2137|Compound:5261020|FD-Score:-2.83|P-value:7.48E-4 ID:SGTC_2281|Compound:7943571|FD-Score:3.09|P-value:1.99E-4 ID:SGTC_2470|Compound:5664817|FD-Score:-3.73|P-value:1.37E-5 ID:SGTC_2487|Compound:5918373|FD-Score:3.37|P-value:5.81E-5 ID:SGTC_2508|Compound:celastrol|FD-Score:-2.84|P-value:7.28E-4 ID:SGTC_2589|Compound:lobaric acid|FD-Score:-3.65|P-value:2.04E-5 ID:SGTC_2660|Compound:2',4'-dihydroxychalcone|FD-Score:2.99|P-value:3.03E-4 ID:SGTC_2750|Compound:bromebric acid|FD-Score:3.08|P-value:2.11E-4 ID:SGTC_2757|Compound:clomiphene|FD-Score:5.27|P-value:9.11E-10 ID:SGTC_2759|Compound:disulfiram|FD-Score:-3.52|P-value:3.77E-5 ID:SGTC_2943|Compound:9068529|FD-Score:-3.46|P-value:4.97E-5 ID:SGTC_2969|Compound:9091408|FD-Score:-3.54|P-value:3.37E-5 ID:SGTC_2971|Compound:9092186|FD-Score:-3.16|P-value:1.90E-4 ID:SGTC_3015|Compound:9080845|FD-Score:3.34|P-value:6.54E-5 ID:SGTC_3291|Compound:9121177|FD-Score:-2.78|P-value:9.15E-4 ID:SGTC_3351|Compound:9150312|FD-Score:-3.42|P-value:5.85E-5 ID:SGTC_3355|Compound:9151058|FD-Score:-2.92|P-value:5.20E-4

Top fitness defect scores for YNL078W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2757 clomiphene 5.27 4.2 µM 9.11E-10 fatty acid desaturase (OLE1) 17 6.81E-8
2 SGTC_2125 5246583 4.82 200.0 µM 1.93E-8 tubulin folding & SWR complex 28 7.34E-7
3 SGTC_1215 3852-0577 4.07 11.2 µM 1.63E-6 15 2.33E-5
4 SGTC_2102 5377816 3.78 72.8 µM 7.89E-6 31 7.97E-5
5 SGTC_1200 1643-0193 3.75 18.4 µM 9.02E-6 24 8.84E-5
6 SGTC_486 niguldipine 3.68 82.0 µM 1.30E-5 amide catabolism 21 1.18E-4
7 SGTC_621 3584-0052 3.65 32.1 µM 1.50E-5 31 1.32E-4
8 SGTC_1180 1193-0155 3.42 285.0 µM 4.62E-5 32 3.17E-4
9 SGTC_2487 5918373 3.37 44.0 µM 5.81E-5 37 3.80E-4
10 SGTC_3015 9080845 3.34 49.5 µM 6.54E-5 22 4.16E-4
11 SGTC_453 enantio-paf c16 3.26 10.0 µM 9.46E-5 211 5.55E-4
12 SGTC_1858 4-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 3.11 62.6 µM 1.85E-4 TSC3-RPN4 37 9.39E-4
13 SGTC_2281 7943571 3.09 200.0 µM 1.99E-4 31 9.94E-4
14 SGTC_2750 bromebric acid 3.08 70.2 µM 2.11E-4 84 0.00104
15 SGTC_2096 5468907 3.08 200.0 µM 2.14E-4 29 0.00105
16 SGTC_40 0327-0103 3.06 19.8 µM 2.27E-4 69 0.00110
17 SGTC_2660 2',4'-dihydroxychalcone 2.99 17.1 µM 3.03E-4 53 0.00138
18 SGTC_1143 3454-2408 2.95 5.1 µM 3.61E-4 60S ribosome export 19 0.00158
19 SGTC_2103 5378138 2.86 10.0 µM 5.19E-4 70 0.00211
20 SGTC_875 0709-0210 2.73 25.7 µM 8.68E-4 fatty acid desaturase (OLE1) 99 0.00315

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.248 2.33E-48 YKL066W_d YKL066W_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene YNK1
0.166 3.99E-22 YKL171W NNK1 hom Protein kinase; implicated in proteasome function; interacts with TORC1, Ure2 and Gdh2; overexpression leads to hypersensitivity to rapamycin and nuclear accumulation of Gln3; epitope-tagged protein localizes to the cytoplasm
0.165 6.55E-22 YOR238W_p YOR238W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.153 5.72E-19 YLR205C HMX1 hom ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants
0.148 7.21E-18 YKL122C SRP21 het Subunit of the signal recognition particle (SRP), which functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm
0.144 5.40E-17 YBL015W ACH1 hom Protein with CoA transferase activity, particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth
0.142 1.14E-16 YKR023W_p YKR023W_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.135 4.79E-15 YCL035C GRX1 hom Glutathione-dependent disulfide oxidoreductase; hydroperoxide and superoxide-radical responsive, heat-stable, with active site cysteine pair; protects cells from oxidative damage; GRX1 has a paralog, GRX2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.133 1.03E-14 YLR241W_p YLR241W_p hom Putative protein of unknown function, may be involved in detoxification
0.131 2.49E-14 YML074C FPR3 hom Nucleolar peptidyl-prolyl cis-trans isomerase (PPIase); FK506 binding protein; phosphorylated by casein kinase II (Cka1p-Cka2p-Ckb1p-Ckb2p) and dephosphorylated by Ptp1p
0.128 8.09E-14 YOR163W DDP1 hom Polyphosphate phosphatase; hydrolyzes diphosphorylated inositol polyphosphates and diadenosine polyphosphates; high specificity for diadenosine hexa- and pentaphosphates; contains endopolyphosphatase activity with a high affinity for polyphosphates, an activity also observed for its human DIPP homologs; possesses mRNA decapping activity; nudix hydrolase family member; protein abundance increases in response to DNA replication stress
0.126 2.27E-13 YMR095C SNO1 hom Protein of unconfirmed function, involved in pyridoxine metabolism; expression is induced during stationary phase; forms a putative glutamine amidotransferase complex with Snz1p, with Sno1p serving as the glutaminase
0.126 2.69E-13 YOR298C-A MBF1 hom Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress
0.126 2.95E-13 YIL010W DOT5 hom Nuclear thiol peroxidase which functions as an alkyl-hydroperoxide reductase during post-diauxic growth
0.125 3.37E-13 YBR263W SHM1 hom Mitochondrial serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis; reverse reaction generates serine