YNL104C / LEU4

Alpha-isopropylmalate synthase (2-isopropylmalate synthase); the main isozyme responsible for the first step in the leucine biosynthesis pathway; LEU4 has a paralog, LEU9, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL104C / LEU4

Click on Significant Values for Screen Details ID:SGTC_113|Compound:0099-0221|FD-Score:3.04|P-value:2.65E-4 ID:SGTC_258|Compound:haloperidol|FD-Score:-3.40|P-value:5.19E-5 ID:SGTC_270|Compound:tamoxifen|FD-Score:3.28|P-value:9.29E-5 ID:SGTC_292|Compound:k038-0015|FD-Score:-2.94|P-value:3.99E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:5.67|P-value:5.13E-11 ID:SGTC_360|Compound:0957-0399|FD-Score:3.44|P-value:4.37E-5 ID:SGTC_361|Compound:000s-0510|FD-Score:-3.83|P-value:6.11E-6 ID:SGTC_396|Compound:dithiazanine|FD-Score:-3.67|P-value:1.39E-5 ID:SGTC_401|Compound:neomycin|FD-Score:-4.12|P-value:1.27E-6 ID:SGTC_405|Compound:ebelactone b|FD-Score:3.36|P-value:6.35E-5 ID:SGTC_534|Compound:k292-0788|FD-Score:3.61|P-value:1.88E-5 ID:SGTC_537|Compound:1334-0398|FD-Score:4.49|P-value:1.52E-7 ID:SGTC_809|Compound:3998-0021|FD-Score:4.27|P-value:5.56E-7 ID:SGTC_1035|Compound:k081-0041|FD-Score:2.80|P-value:7.06E-4 ID:SGTC_1305|Compound:1181-0477|FD-Score:2.74|P-value:8.72E-4 ID:SGTC_1315|Compound:1237-0019|FD-Score:3.11|P-value:1.96E-4 ID:SGTC_1322|Compound:1348-1070|FD-Score:2.75|P-value:8.50E-4 ID:SGTC_1356|Compound:1502-0781|FD-Score:2.90|P-value:4.67E-4 ID:SGTC_1724|Compound:st037281|FD-Score:-2.95|P-value:3.80E-4 ID:SGTC_1802|Compound:st050112|FD-Score:-2.93|P-value:4.14E-4 ID:SGTC_1908|Compound:st060353|FD-Score:2.73|P-value:9.11E-4 ID:SGTC_2013|Compound:4024628|FD-Score:-3.46|P-value:3.88E-5 ID:SGTC_2022|Compound:5136654|FD-Score:3.88|P-value:4.83E-6 ID:SGTC_2467|Compound:5474540|FD-Score:-3.65|P-value:1.57E-5 ID:SGTC_2601|Compound:securinine|FD-Score:-3.87|P-value:5.14E-6 ID:SGTC_2625|Compound:euphol|FD-Score:3.02|P-value:2.86E-4 ID:SGTC_2829|Compound:7992743|FD-Score:2.71|P-value:9.80E-4 ID:SGTC_2830|Compound:7992007|FD-Score:-3.63|P-value:1.75E-5 ID:SGTC_2832|Compound:7992762|FD-Score:-4.31|P-value:4.35E-7 ID:SGTC_2834|Compound:9001755|FD-Score:4.94|P-value:8.87E-9 ID:SGTC_2864|Compound:9034755|FD-Score:-3.98|P-value:2.85E-6 ID:SGTC_2873|Compound:9040596|FD-Score:2.77|P-value:7.97E-4 ID:SGTC_3072|Compound:9116419|FD-Score:-4.18|P-value:9.42E-7 ID:SGTC_3082|Compound:9117008|FD-Score:-3.18|P-value:1.45E-4 ID:SGTC_3083|Compound:9117465|FD-Score:2.74|P-value:8.98E-4 ID:SGTC_3084|Compound:9116877|FD-Score:3.13|P-value:1.79E-4 ID:SGTC_3201|Compound:9113999|FD-Score:-3.08|P-value:2.25E-4 ID:SGTC_3285|Compound:9112818|FD-Score:-3.81|P-value:6.89E-6 ID:SGTC_3329|Compound:9141233|FD-Score:3.47|P-value:3.80E-5 ID:SGTC_113|Compound:0099-0221|FD-Score:3.04|P-value:2.65E-4 ID:SGTC_258|Compound:haloperidol|FD-Score:-3.40|P-value:5.19E-5 ID:SGTC_270|Compound:tamoxifen|FD-Score:3.28|P-value:9.29E-5 ID:SGTC_292|Compound:k038-0015|FD-Score:-2.94|P-value:3.99E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:5.67|P-value:5.13E-11 ID:SGTC_360|Compound:0957-0399|FD-Score:3.44|P-value:4.37E-5 ID:SGTC_361|Compound:000s-0510|FD-Score:-3.83|P-value:6.11E-6 ID:SGTC_396|Compound:dithiazanine|FD-Score:-3.67|P-value:1.39E-5 ID:SGTC_401|Compound:neomycin|FD-Score:-4.12|P-value:1.27E-6 ID:SGTC_405|Compound:ebelactone b|FD-Score:3.36|P-value:6.35E-5 ID:SGTC_534|Compound:k292-0788|FD-Score:3.61|P-value:1.88E-5 ID:SGTC_537|Compound:1334-0398|FD-Score:4.49|P-value:1.52E-7 ID:SGTC_809|Compound:3998-0021|FD-Score:4.27|P-value:5.56E-7 ID:SGTC_1035|Compound:k081-0041|FD-Score:2.80|P-value:7.06E-4 ID:SGTC_1305|Compound:1181-0477|FD-Score:2.74|P-value:8.72E-4 ID:SGTC_1315|Compound:1237-0019|FD-Score:3.11|P-value:1.96E-4 ID:SGTC_1322|Compound:1348-1070|FD-Score:2.75|P-value:8.50E-4 ID:SGTC_1356|Compound:1502-0781|FD-Score:2.90|P-value:4.67E-4 ID:SGTC_1724|Compound:st037281|FD-Score:-2.95|P-value:3.80E-4 ID:SGTC_1802|Compound:st050112|FD-Score:-2.93|P-value:4.14E-4 ID:SGTC_1908|Compound:st060353|FD-Score:2.73|P-value:9.11E-4 ID:SGTC_2013|Compound:4024628|FD-Score:-3.46|P-value:3.88E-5 ID:SGTC_2022|Compound:5136654|FD-Score:3.88|P-value:4.83E-6 ID:SGTC_2467|Compound:5474540|FD-Score:-3.65|P-value:1.57E-5 ID:SGTC_2601|Compound:securinine|FD-Score:-3.87|P-value:5.14E-6 ID:SGTC_2625|Compound:euphol|FD-Score:3.02|P-value:2.86E-4 ID:SGTC_2829|Compound:7992743|FD-Score:2.71|P-value:9.80E-4 ID:SGTC_2830|Compound:7992007|FD-Score:-3.63|P-value:1.75E-5 ID:SGTC_2832|Compound:7992762|FD-Score:-4.31|P-value:4.35E-7 ID:SGTC_2834|Compound:9001755|FD-Score:4.94|P-value:8.87E-9 ID:SGTC_2864|Compound:9034755|FD-Score:-3.98|P-value:2.85E-6 ID:SGTC_2873|Compound:9040596|FD-Score:2.77|P-value:7.97E-4 ID:SGTC_3072|Compound:9116419|FD-Score:-4.18|P-value:9.42E-7 ID:SGTC_3082|Compound:9117008|FD-Score:-3.18|P-value:1.45E-4 ID:SGTC_3083|Compound:9117465|FD-Score:2.74|P-value:8.98E-4 ID:SGTC_3084|Compound:9116877|FD-Score:3.13|P-value:1.79E-4 ID:SGTC_3201|Compound:9113999|FD-Score:-3.08|P-value:2.25E-4 ID:SGTC_3285|Compound:9112818|FD-Score:-3.81|P-value:6.89E-6 ID:SGTC_3329|Compound:9141233|FD-Score:3.47|P-value:3.80E-5

Top fitness defect scores for YNL104C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_358 0075-0058 5.67 9.4 µM 5.13E-11 22 7.31E-9
2 SGTC_2834 9001755 4.94 58.4 µM 8.87E-9 17 3.94E-7
3 SGTC_537 1334-0398 4.49 209.0 µM 1.52E-7 14 3.57E-6
4 SGTC_809 3998-0021 4.27 90.0 µM 5.56E-7 RNA processing & uracil transport 10 9.78E-6
5 SGTC_2022 5136654 3.88 200.0 µM 4.83E-6 23 5.24E-5
6 SGTC_534 k292-0788 3.61 63.4 µM 1.88E-5 mitochondrial processes 39 1.51E-4
7 SGTC_3329 9141233 3.47 27.6 µM 3.80E-5 23 2.62E-4
8 SGTC_360 0957-0399 3.44 1.0 µM 4.37E-5 cell wall 68 2.92E-4
9 SGTC_405 ebelactone b 3.36 50.0 µM 6.35E-5 38 3.90E-4
10 SGTC_270 tamoxifen 3.28 5.5 µM 9.29E-5 NEO1 64 5.26E-4
11 SGTC_3084 9116877 3.13 49.5 µM 1.79E-4 21 8.76E-4
12 SGTC_1315 1237-0019 3.11 3.7 µM 1.96E-4 34 9.42E-4
13 SGTC_113 0099-0221 3.04 2.5 µM 2.65E-4 71 0.00119
14 SGTC_2625 euphol 3.02 100.0 µM 2.86E-4 78 0.00127
15 SGTC_1356 1502-0781 2.90 85.8 µM 4.67E-4 fatty acid desaturase (OLE1) 66 0.00186
16 SGTC_1035 k081-0041 2.80 55.2 µM 7.06E-4 Golgi 91 0.00257
17 SGTC_2873 9040596 2.77 71.4 µM 7.97E-4 121 0.00282
18 SGTC_1322 1348-1070 2.75 12.0 µM 8.50E-4 44 0.00297
19 SGTC_1305 1181-0477 2.74 144.0 µM 8.72E-4 76 0.00303
20 SGTC_3083 9117465 2.74 49.5 µM 8.98E-4 104 0.00310

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.191 7.09E-29 YDR420W HKR1 hom Mucin family member that functions as an osmosensor in the Sho1p-mediated HOG pathway with Msb2p; proposed to be a negative regulator of filamentous growth; mutant displays defects in beta-1,3 glucan synthesis and bud site selection
0.151 1.61E-18 YBL002W HTB2 hom Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB1; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation
0.133 1.31E-14 YLR237W THI7 hom Plasma membrane transporter responsible for the uptake of thiamine, member of the major facilitator superfamily of transporters; mutation of human ortholog causes thiamine-responsive megaloblastic anemia
0.132 1.65E-14 YMR140W SIP5 hom Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase; forms cytoplasmic foci upon DNA replication stress
0.114 3.22E-11 YJL217W REE1 hom Cytoplasmic protein involved in the regulation of enolase (ENO1); mRNA expression is induced by calcium shortage, copper deficiency (via Mac1p) and the presence of galactose (via Gal4p); mRNA expression is also regulated by the cell cycle
0.111 1.08E-10 YNL049C SFB2 hom Component of the Sec23p-Sfb2p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb3p
0.108 3.23E-10 YJR115W_p YJR115W_p hom Putative protein of unknown function; YJR115W has a paralog, ECM13, that arose from the whole genome duplication
0.106 7.23E-10 YKL106C-A_p YKL106C-A_p hom Putative protein of unknown function; identified by homology to uncharacterized proteins in other fungi
0.106 6.94E-10 YAL020C ATS1 hom Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle
0.105 9.62E-10 YGR192C TDH3 hom Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; TDH3 has a paralog, TDH2, that arose from the whole genome duplication
0.102 3.58E-9 YAL013W DEP1 hom Transcriptional modulator involved in regulation of structural phospholipid biosynthesis genes and metabolically unrelated genes, as well as maintenance of telomeres, mating efficiency, and sporulation
0.098 1.24E-8 YPL273W SAM4 hom S-adenosylmethionine-homocysteine methyltransferase, functions along with Mht1p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio
0.097 1.78E-8 YOL071W EMI5 hom Subunit of succinate dehydrogenase, which couples succinate oxidation to ubiquinone reduction; required for FAD cofactor attachment to Sdh1p; mutations in human ortholog PGL2 are associated with neuroendocrine tumors (paraganglioma)
0.097 1.95E-8 YDR528W HLR1 hom Protein involved in regulation of cell wall composition and integrity and response to osmotic stress; overproduction suppresses a lysis sensitive PKC mutation; similar to Lre1p, which functions antagonistically to protein kinase A;
HLR1 has a paralog, LRE1, that arose from the whole genome duplication
0.096 2.20E-8 YDR476C_p YDR476C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene