YNL146W_p

Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNL146W is not an essential gene

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL146W / YNL146W

Click on Significant Values for Screen Details ID:SGTC_72|Compound:0744-0168|FD-Score:-3.46|P-value:1.70E-4 ID:SGTC_77|Compound:0828-0286|FD-Score:4.08|P-value:1.61E-5 ID:SGTC_150|Compound:3970-0969|FD-Score:4.84|P-value:4.01E-7 ID:SGTC_307|Compound:1501-1223|FD-Score:3.20|P-value:5.80E-4 ID:SGTC_318|Compound:1488-0629|FD-Score:3.07|P-value:9.23E-4 ID:SGTC_371|Compound:0195-0006|FD-Score:3.25|P-value:4.84E-4 ID:SGTC_387|Compound:norethindrone acetate|FD-Score:-4.63|P-value:8.43E-7 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:-3.02|P-value:8.69E-4 ID:SGTC_424|Compound:splitomicin|FD-Score:3.13|P-value:7.25E-4 ID:SGTC_429|Compound:1112-0049|FD-Score:-3.20|P-value:4.59E-4 ID:SGTC_446|Compound:go6976|FD-Score:3.29|P-value:4.08E-4 ID:SGTC_450|Compound:aminopyrene|FD-Score:-3.01|P-value:8.97E-4 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:3.64|P-value:1.07E-4 ID:SGTC_582|Compound:0987-0078|FD-Score:3.84|P-value:4.70E-5 ID:SGTC_686|Compound:1319-0111|FD-Score:5.03|P-value:1.44E-7 ID:SGTC_984|Compound:1492-0255|FD-Score:-3.01|P-value:8.81E-4 ID:SGTC_1076|Compound:itraconazole|FD-Score:3.13|P-value:7.44E-4 ID:SGTC_1204|Compound:4049-0315|FD-Score:4.76|P-value:6.06E-7 ID:SGTC_1279|Compound:0874-0590|FD-Score:3.83|P-value:4.89E-5 ID:SGTC_1339|Compound:1486-1321|FD-Score:3.54|P-value:1.59E-4 ID:SGTC_1415|Compound:3966-0071|FD-Score:3.60|P-value:1.26E-4 ID:SGTC_1541|Compound:2',3',6-trimethoxyflavone|FD-Score:3.53|P-value:1.67E-4 ID:SGTC_1649|Compound:st011737|FD-Score:4.15|P-value:1.19E-5 ID:SGTC_1676|Compound:st018489|FD-Score:-3.88|P-value:2.94E-5 ID:SGTC_1772|Compound:st048439|FD-Score:3.24|P-value:4.94E-4 ID:SGTC_2017|Compound:5132927|FD-Score:3.05|P-value:9.73E-4 ID:SGTC_2126|Compound:5251174|FD-Score:-3.06|P-value:7.37E-4 ID:SGTC_2284|Compound:7945075|FD-Score:3.91|P-value:3.45E-5 ID:SGTC_2301|Compound:7769476|FD-Score:-3.08|P-value:6.87E-4 ID:SGTC_2303|Compound:7774051|FD-Score:3.04|P-value:9.96E-4 ID:SGTC_2308|Compound:7584454|FD-Score:3.30|P-value:3.96E-4 ID:SGTC_2339|Compound:9000433|FD-Score:-4.55|P-value:1.24E-6 ID:SGTC_2393|Compound:9031399|FD-Score:6.05|P-value:3.31E-10 ID:SGTC_2415|Compound:st077333|FD-Score:3.41|P-value:2.64E-4 ID:SGTC_2528|Compound:xanthyletin|FD-Score:-3.31|P-value:3.00E-4 ID:SGTC_2532|Compound:xanthone|FD-Score:3.14|P-value:7.02E-4 ID:SGTC_2837|Compound:9008476|FD-Score:3.53|P-value:1.64E-4 ID:SGTC_2854|Compound:9024559|FD-Score:4.06|P-value:1.78E-5 ID:SGTC_2978|Compound:9011784|FD-Score:3.35|P-value:3.33E-4 ID:SGTC_72|Compound:0744-0168|FD-Score:-3.46|P-value:1.70E-4 ID:SGTC_77|Compound:0828-0286|FD-Score:4.08|P-value:1.61E-5 ID:SGTC_150|Compound:3970-0969|FD-Score:4.84|P-value:4.01E-7 ID:SGTC_307|Compound:1501-1223|FD-Score:3.20|P-value:5.80E-4 ID:SGTC_318|Compound:1488-0629|FD-Score:3.07|P-value:9.23E-4 ID:SGTC_371|Compound:0195-0006|FD-Score:3.25|P-value:4.84E-4 ID:SGTC_387|Compound:norethindrone acetate|FD-Score:-4.63|P-value:8.43E-7 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:-3.02|P-value:8.69E-4 ID:SGTC_424|Compound:splitomicin|FD-Score:3.13|P-value:7.25E-4 ID:SGTC_429|Compound:1112-0049|FD-Score:-3.20|P-value:4.59E-4 ID:SGTC_446|Compound:go6976|FD-Score:3.29|P-value:4.08E-4 ID:SGTC_450|Compound:aminopyrene|FD-Score:-3.01|P-value:8.97E-4 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:3.64|P-value:1.07E-4 ID:SGTC_582|Compound:0987-0078|FD-Score:3.84|P-value:4.70E-5 ID:SGTC_686|Compound:1319-0111|FD-Score:5.03|P-value:1.44E-7 ID:SGTC_984|Compound:1492-0255|FD-Score:-3.01|P-value:8.81E-4 ID:SGTC_1076|Compound:itraconazole|FD-Score:3.13|P-value:7.44E-4 ID:SGTC_1204|Compound:4049-0315|FD-Score:4.76|P-value:6.06E-7 ID:SGTC_1279|Compound:0874-0590|FD-Score:3.83|P-value:4.89E-5 ID:SGTC_1339|Compound:1486-1321|FD-Score:3.54|P-value:1.59E-4 ID:SGTC_1415|Compound:3966-0071|FD-Score:3.60|P-value:1.26E-4 ID:SGTC_1541|Compound:2',3',6-trimethoxyflavone|FD-Score:3.53|P-value:1.67E-4 ID:SGTC_1649|Compound:st011737|FD-Score:4.15|P-value:1.19E-5 ID:SGTC_1676|Compound:st018489|FD-Score:-3.88|P-value:2.94E-5 ID:SGTC_1772|Compound:st048439|FD-Score:3.24|P-value:4.94E-4 ID:SGTC_2017|Compound:5132927|FD-Score:3.05|P-value:9.73E-4 ID:SGTC_2126|Compound:5251174|FD-Score:-3.06|P-value:7.37E-4 ID:SGTC_2284|Compound:7945075|FD-Score:3.91|P-value:3.45E-5 ID:SGTC_2301|Compound:7769476|FD-Score:-3.08|P-value:6.87E-4 ID:SGTC_2303|Compound:7774051|FD-Score:3.04|P-value:9.96E-4 ID:SGTC_2308|Compound:7584454|FD-Score:3.30|P-value:3.96E-4 ID:SGTC_2339|Compound:9000433|FD-Score:-4.55|P-value:1.24E-6 ID:SGTC_2393|Compound:9031399|FD-Score:6.05|P-value:3.31E-10 ID:SGTC_2415|Compound:st077333|FD-Score:3.41|P-value:2.64E-4 ID:SGTC_2528|Compound:xanthyletin|FD-Score:-3.31|P-value:3.00E-4 ID:SGTC_2532|Compound:xanthone|FD-Score:3.14|P-value:7.02E-4 ID:SGTC_2837|Compound:9008476|FD-Score:3.53|P-value:1.64E-4 ID:SGTC_2854|Compound:9024559|FD-Score:4.06|P-value:1.78E-5 ID:SGTC_2978|Compound:9011784|FD-Score:3.35|P-value:3.33E-4

Top fitness defect scores for YNL146W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
1 SGTC_2393 9031399 6.05 185.9 µM 3.31E-10 RNA processing & uracil transport 5 7.39E-10
2 SGTC_686 1319-0111 5.03 510.0 µM 1.44E-7 60S ribosome export 9 2.45E-7
3 SGTC_150 3970-0969 4.84 77.2 µM 4.01E-7 37 6.53E-7
4 SGTC_1204 4049-0315 4.76 18.3 µM 6.06E-7 8 9.70E-7
5 SGTC_1649 st011737 4.15 51.3 µM 1.19E-5 27 1.68E-5
6 SGTC_77 0828-0286 4.08 12.5 µM 1.61E-5 TSC3-RPN4 32 2.24E-5
7 SGTC_2854 9024559 4.06 71.4 µM 1.78E-5 17 2.46E-5
8 SGTC_2284 7945075 3.91 39.2 µM 3.45E-5 mitochondrial processes 27 4.65E-5
9 SGTC_582 0987-0078 3.84 156.0 µM 4.70E-5 calcium & mitochondrial duress 78 6.25E-5
10 SGTC_1279 0874-0590 3.83 65.2 µM 4.89E-5 35 6.49E-5
11 SGTC_474 dl-dihydrosphingosine 3.64 5.0 µM 1.07E-4 33 1.38E-4
12 SGTC_1415 3966-0071 3.60 78.1 µM 1.26E-4 DNA intercalators 17 1.61E-4
13 SGTC_1339 1486-1321 3.54 22.7 µM 1.59E-4 ERG2 39 2.00E-4
14 SGTC_2837 9008476 3.53 45.5 µM 1.64E-4 51 2.08E-4
15 SGTC_1541 2',3',6-trimethoxyflavone 3.53 64.0 µM 1.67E-4 23 2.10E-4
16 SGTC_2415 st077333 3.41 57.7 µM 2.64E-4 76 3.27E-4
17 SGTC_2978 9011784 3.35 43.0 µM 3.33E-4 57 4.09E-4
18 SGTC_2308 7584454 3.30 197.6 µM 3.96E-4 52 4.83E-4
19 SGTC_446 go6976 3.29 132.0 µM 4.08E-4 33 4.96E-4
20 SGTC_371 0195-0006 3.25 7.4 µM 4.84E-4 42 5.85E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.118 6.78E-12 YAL061W_p BDH2_p hom Putative medium-chain alcohol dehydrogenase with similarity to BDH1; transcription induced by constitutively active PDR1 and PDR3
0.109 2.08E-10 YIL105C SLM1 hom Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; protein abundance increases in response to DNA replication stress; SLM1 has a paralog, SLM2, that arose from the whole genome duplication
0.084 1.01E-6 YKL129C MYO3 hom One of two type I myosins; localizes to actin cortical patches; deletion of MYO3 has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization
0.083 1.41E-6 YHR065C RRP3 het Protein involved in rRNA processing; required for maturation of the 35S primary transcript of pre-rRNA and for cleavage leading to mature 18S rRNA; homologous to eIF-4a, which is a DEAD box RNA-dependent ATPase with helicase activity
0.082 1.92E-6 YNR019W ARE2 hom Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the presence of oxygen; ARE2 has a paralog, ARE1, that arose from the whole genome duplication
0.082 2.09E-6 YNR046W TRM112 het Protein involved in methylation of tRNA, rRNA, and translation factors; subunit of tRNA methyltransferase (MTase) complexes in combination with Trm9p and Trm11p; interacts with and stabilizes 18S rRNA methyltransferase Bud23p; subunit of complex with Mtq2p that methylates Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; deletion confers resistance to zymocin; relative distribution to the nucleus increases upon DNA replication stress
0.080 3.51E-6 YDR128W MTC5 hom Subunit of the SEA (Seh1-associated) complex; SEA is a coatomer-related complex that associates dynamically with the vacuole; has N-terminal WD-40 repeats and a C-terminal RING motif; mtc5 is synthetically sick with cdc13-1; relative distribution to vacuolar membrane punctae decreases upon DNA replication stress
0.076 9.39E-6 YBR215W HPC2 hom Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; mutants display synthetic defects with subunits of FACT, a complex that allows passage of RNA Pol II through nucleosomes
0.075 1.24E-5 YBR021W FUR4 hom Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation
0.075 1.49E-5 YLR182W SWI6 hom Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators
0.072 2.75E-5 YLL042C ATG10 hom Conserved E2-like conjugating enzyme that mediates formation of the Atg12p-Atg5p conjugate, which is a critical step in autophagy
0.072 2.78E-5 YLR460C_p YLR460C_p hom Member of the quinone oxidoreductase family, up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine
0.070 4.59E-5 YPL163C SVS1 hom Cell wall and vacuolar protein; required for wild-type resistance to vanadate; SVS1 has a paralog, SRL1, that arose from the whole genome duplication
0.070 4.93E-5 YOR277C_d YOR277C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene CAF20
0.069 6.43E-5 YHR180W_d YHR180W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data