HIPHOP chemogenomics database

YNL219C / ALG9

Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation

Zygosity: Homozygous strain

fixedexpanded
Profile for YNL219C / ALG9

Click on Significant Values for Screen Details

Top fitness defect scores for YNL219C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_312	4092-0653	6.77	6.8 µM	5.79E-11	ergosterol depletion effects on membrane	26	6.59E-12
2	SGTC_2688	kanamycin b	6.13	96.7 µM	2.69E-9	calcium & mitochondrial duress	151	4.46E-10
3	SGTC_2009	4012297	5.30	12.1 µM	2.22E-7	cell wall signaling	34	5.67E-8
4	SGTC_2967	9089538	5.29	49.9 µM	2.36E-7		9	6.08E-8
5	SGTC_2896	9052656	5.23	71.4 µM	3.24E-7	endomembrane recycling	52	8.60E-8
6	SGTC_1713	st033146	4.89	12.8 µM	1.60E-6		11	4.95E-7
7	SGTC_2552	juarezic acid	4.85	87.0 µM	1.94E-6	TSC3-RPN4	14	6.15E-7
8	SGTC_568	1193-0097	4.79	7.5 µM	2.57E-6	ERAD & cell cycle	97	8.33E-7
9	SGTC_2413	inermin	4.72	26.1 µM	3.54E-6		34	1.19E-6
10	SGTC_2021	4019055	4.40	173.0 µM	1.39E-5	endomembrane recycling	97	5.31E-6
11	SGTC_800	3448-7362	4.24	155.0 µM	2.76E-5	translation	139	1.13E-5
12	SGTC_860	0443-0016	4.21	25.1 µM	3.15E-5		25	1.31E-5
13	SGTC_1789	tunicamycin	4.13	25.0 nM	4.22E-5	unfolded protein response	40	1.80E-5
14	SGTC_1597	7-benzyloxygramine	4.09	47.5 µM	4.97E-5	calcium & mitochondrial duress	113	2.15E-5
15	SGTC_3108	9121785	4.03	49.5 µM	6.28E-5		12	2.78E-5
16	SGTC_143	4032-1194	4.02	15.7 µM	6.50E-5		62	2.89E-5
17	SGTC_637	1181-0519	4.00	7.3 µM	7.00E-5	fatty acid desaturase (OLE1)	100	3.13E-5
18	SGTC_3037	9091026	3.87	49.5 µM	1.17E-4	NEO1-PIK1	128	5.48E-5
19	SGTC_255	tunicamycin	3.86	200.5 nM	1.19E-4	unfolded protein response	77	5.58E-5
20	SGTC_776	4476-4378	3.74	112.0 µM	1.84E-4	ERG2	24	9.06E-5

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.248	2.27E-48	YBL082C	ALG3	hom	Dolichol-P-Man dependent alpha(1-3) mannosyltransferase, involved in the synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins
0.209	1.90E-34	YGR206W	MVB12	hom	ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin
0.201	5.28E-32	YOR002W	ALG6	hom	Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease
0.201	6.71E-32	YDR156W	RPA14	hom	RNA polymerase I subunit A14
0.199	3.97E-31	YDL096C_d	OPI6_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential
0.189	2.20E-28	YBR243C	ALG7	het	UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin
0.189	2.28E-28	YJR075W	HOC1	hom	Alpha-1,6-mannosyltransferase involved in cell wall mannan biosynthesis; subunit of a Golgi-localized complex that also contains Anp1p, Mnn9p, Mnn11p, and Mnn10p; identified as a suppressor of a cell lysis sensitive pkc1-371 allele
0.182	1.55E-26	YEL022W	GEA2	hom	Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication
0.181	2.94E-26	YML019W	OST6	hom	Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p
0.180	7.14E-26	YBL083C_d	YBL083C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3
0.179	1.12E-25	YDR466W	PKH3	hom	Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant
0.174	3.92E-24	YFR040W	SAP155	hom	Protein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress
0.171	1.61E-23	YDR101C	ARX1	hom	Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit biogenesis; interacts directly with Alb1; responsible for Tif6 recycling defects in absence of Rei1; associated with the ribosomal export complex
0.166	4.00E-22	YKL074C	MUD2	hom	Protein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; similar to metazoan splicing factor U2AF65
0.165	8.85E-22	YKL051W	SFK1	hom	Plasma membrane protein that may act together with or upstream of Stt4p to generate normal levels of the essential phospholipid PI4P, at least partially mediates proper localization of Stt4p to the plasma membrane