YNL273W / TOF1

Subunit of a replication-pausing checkpoint complex (Tof1p-Mrc1p-Csm3p) that acts at the stalled replication fork to promote sister chromatid cohesion after DNA damage, facilitating gap repair of damaged DNA; interacts with the MCM helicase

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL273W / TOF1

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:2.96|P-value:8.93E-4 ID:SGTC_69|Compound:0096-0274|FD-Score:4.26|P-value:3.44E-6 ID:SGTC_93|Compound:0109-0045|FD-Score:2.94|P-value:9.74E-4 ID:SGTC_129|Compound:0088-0017|FD-Score:3.04|P-value:6.68E-4 ID:SGTC_188|Compound:3229-0966|FD-Score:3.14|P-value:4.65E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:5.53|P-value:2.58E-9 ID:SGTC_514|Compound:4466-0038|FD-Score:5.50|P-value:3.07E-9 ID:SGTC_558|Compound:k064-0027|FD-Score:3.45|P-value:1.40E-4 ID:SGTC_661|Compound:1304-0007|FD-Score:4.71|P-value:3.32E-7 ID:SGTC_784|Compound:4579-0127|FD-Score:3.61|P-value:6.98E-5 ID:SGTC_790|Compound:1575-0012|FD-Score:4.01|P-value:1.16E-5 ID:SGTC_996|Compound:3970-0799|FD-Score:9.15|P-value:1.92E-22 ID:SGTC_1046|Compound:2001-0062|FD-Score:-4.64|P-value:4.00E-7 ID:SGTC_1056|Compound:3448-1962|FD-Score:-4.19|P-value:4.09E-6 ID:SGTC_1079|Compound:idarubicin|FD-Score:4.52|P-value:8.99E-7 ID:SGTC_1169|Compound:aniline yellow|FD-Score:2.94|P-value:9.62E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:2.98|P-value:8.35E-4 ID:SGTC_1379|Compound:2144-0755|FD-Score:3.38|P-value:1.83E-4 ID:SGTC_1409|Compound:3909-8004|FD-Score:2.96|P-value:8.98E-4 ID:SGTC_1448|Compound:4255-0083|FD-Score:3.38|P-value:1.83E-4 ID:SGTC_1555|Compound:citronellol|FD-Score:-3.22|P-value:3.03E-4 ID:SGTC_1571|Compound:st070080|FD-Score:-3.92|P-value:1.52E-5 ID:SGTC_1640|Compound:st009078|FD-Score:2.99|P-value:8.04E-4 ID:SGTC_1707|Compound:st032323|FD-Score:5.92|P-value:1.92E-10 ID:SGTC_1757|Compound:st041620|FD-Score:3.56|P-value:8.74E-5 ID:SGTC_1909|Compound:st060837|FD-Score:-3.17|P-value:3.66E-4 ID:SGTC_2004|Compound:4023728|FD-Score:3.22|P-value:3.47E-4 ID:SGTC_2016|Compound:5121758|FD-Score:-4.35|P-value:1.89E-6 ID:SGTC_2083|Compound:5306046|FD-Score:4.54|P-value:8.16E-7 ID:SGTC_2150|Compound:5556613|FD-Score:2.93|P-value:9.98E-4 ID:SGTC_2156|Compound:5649887|FD-Score:3.16|P-value:4.26E-4 ID:SGTC_2367|Compound:9070478|FD-Score:2.97|P-value:8.81E-4 ID:SGTC_2376|Compound:9072922|FD-Score:-3.32|P-value:2.06E-4 ID:SGTC_2392|Compound:N-(3-chlorophenyl)-6-oxo-1H-pyridine-3-carboxamide|FD-Score:3.26|P-value:2.93E-4 ID:SGTC_2415|Compound:st077333|FD-Score:3.37|P-value:1.93E-4 ID:SGTC_2532|Compound:xanthone|FD-Score:2.94|P-value:9.73E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:4.25|P-value:3.54E-6 ID:SGTC_2619|Compound:obliquin|FD-Score:3.06|P-value:6.36E-4 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:-3.87|P-value:1.93E-5 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:-4.81|P-value:1.64E-7 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:-3.10|P-value:4.77E-4 ID:SGTC_2956|Compound:9076196|FD-Score:-3.00|P-value:7.08E-4 ID:SGTC_3093|Compound:9117344|FD-Score:4.74|P-value:2.76E-7 ID:SGTC_58|Compound:0957-0393|FD-Score:2.96|P-value:8.93E-4 ID:SGTC_69|Compound:0096-0274|FD-Score:4.26|P-value:3.44E-6 ID:SGTC_93|Compound:0109-0045|FD-Score:2.94|P-value:9.74E-4 ID:SGTC_129|Compound:0088-0017|FD-Score:3.04|P-value:6.68E-4 ID:SGTC_188|Compound:3229-0966|FD-Score:3.14|P-value:4.65E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:5.53|P-value:2.58E-9 ID:SGTC_514|Compound:4466-0038|FD-Score:5.50|P-value:3.07E-9 ID:SGTC_558|Compound:k064-0027|FD-Score:3.45|P-value:1.40E-4 ID:SGTC_661|Compound:1304-0007|FD-Score:4.71|P-value:3.32E-7 ID:SGTC_784|Compound:4579-0127|FD-Score:3.61|P-value:6.98E-5 ID:SGTC_790|Compound:1575-0012|FD-Score:4.01|P-value:1.16E-5 ID:SGTC_996|Compound:3970-0799|FD-Score:9.15|P-value:1.92E-22 ID:SGTC_1046|Compound:2001-0062|FD-Score:-4.64|P-value:4.00E-7 ID:SGTC_1056|Compound:3448-1962|FD-Score:-4.19|P-value:4.09E-6 ID:SGTC_1079|Compound:idarubicin|FD-Score:4.52|P-value:8.99E-7 ID:SGTC_1169|Compound:aniline yellow|FD-Score:2.94|P-value:9.62E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:2.98|P-value:8.35E-4 ID:SGTC_1379|Compound:2144-0755|FD-Score:3.38|P-value:1.83E-4 ID:SGTC_1409|Compound:3909-8004|FD-Score:2.96|P-value:8.98E-4 ID:SGTC_1448|Compound:4255-0083|FD-Score:3.38|P-value:1.83E-4 ID:SGTC_1555|Compound:citronellol|FD-Score:-3.22|P-value:3.03E-4 ID:SGTC_1571|Compound:st070080|FD-Score:-3.92|P-value:1.52E-5 ID:SGTC_1640|Compound:st009078|FD-Score:2.99|P-value:8.04E-4 ID:SGTC_1707|Compound:st032323|FD-Score:5.92|P-value:1.92E-10 ID:SGTC_1757|Compound:st041620|FD-Score:3.56|P-value:8.74E-5 ID:SGTC_1909|Compound:st060837|FD-Score:-3.17|P-value:3.66E-4 ID:SGTC_2004|Compound:4023728|FD-Score:3.22|P-value:3.47E-4 ID:SGTC_2016|Compound:5121758|FD-Score:-4.35|P-value:1.89E-6 ID:SGTC_2083|Compound:5306046|FD-Score:4.54|P-value:8.16E-7 ID:SGTC_2150|Compound:5556613|FD-Score:2.93|P-value:9.98E-4 ID:SGTC_2156|Compound:5649887|FD-Score:3.16|P-value:4.26E-4 ID:SGTC_2367|Compound:9070478|FD-Score:2.97|P-value:8.81E-4 ID:SGTC_2376|Compound:9072922|FD-Score:-3.32|P-value:2.06E-4 ID:SGTC_2392|Compound:N-(3-chlorophenyl)-6-oxo-1H-pyridine-3-carboxamide|FD-Score:3.26|P-value:2.93E-4 ID:SGTC_2415|Compound:st077333|FD-Score:3.37|P-value:1.93E-4 ID:SGTC_2532|Compound:xanthone|FD-Score:2.94|P-value:9.73E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:4.25|P-value:3.54E-6 ID:SGTC_2619|Compound:obliquin|FD-Score:3.06|P-value:6.36E-4 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:-3.87|P-value:1.93E-5 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:-4.81|P-value:1.64E-7 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:-3.10|P-value:4.77E-4 ID:SGTC_2956|Compound:9076196|FD-Score:-3.00|P-value:7.08E-4 ID:SGTC_3093|Compound:9117344|FD-Score:4.74|P-value:2.76E-7

Top fitness defect scores for YNL273W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_996 3970-0799 9.15 1.8 µM 1.92E-22 17 2.89E-20
2 SGTC_1707 st032323 5.92 6.3 µM 1.92E-10 DNA damage response 21 1.56E-9
3 SGTC_268 camptothecin 5.53 39.6 µM 2.58E-9 DNA damage response 36 1.60E-8
4 SGTC_514 4466-0038 5.50 2.6 nM 3.07E-9 cell wall 92 1.88E-8
5 SGTC_3093 9117344 4.74 49.5 µM 2.76E-7 60S ribosome export 6 1.06E-6
6 SGTC_661 1304-0007 4.71 33.6 µM 3.32E-7 10 1.25E-6
7 SGTC_2083 5306046 4.54 65.9 µM 8.16E-7 11 2.80E-6
8 SGTC_1079 idarubicin 4.52 2.4 µM 8.99E-7 DNA damage response 13 3.05E-6
9 SGTC_69 0096-0274 4.26 206.7 µM 3.44E-6 mitochondrial processes 32 1.02E-5
10 SGTC_2584 androsterone 4.25 48.7 µM 3.54E-6 cell wall 111 1.05E-5
11 SGTC_790 1575-0012 4.01 79.1 µM 1.16E-5 TSC3-RPN4 28 3.05E-5
12 SGTC_784 4579-0127 3.61 173.0 µM 6.98E-5 34 1.53E-4
13 SGTC_1757 st041620 3.56 31.8 µM 8.74E-5 40 1.87E-4
14 SGTC_558 k064-0027 3.45 21.3 µM 1.40E-4 DNA damage response 42 2.86E-4
15 SGTC_1379 2144-0755 3.38 36.5 µM 1.83E-4 36 3.63E-4
16 SGTC_1448 4255-0083 3.38 152.0 µM 1.83E-4 ERG2 33 3.64E-4
17 SGTC_2415 st077333 3.37 57.7 µM 1.93E-4 83 3.82E-4
18 SGTC_2392 N-(3-chlorophenyl)-6-oxo-1H-pyridine-3-carboxamide 3.26 200.0 µM 2.93E-4 29 5.55E-4
19 SGTC_2004 4023728 3.22 41.9 µM 3.47E-4 59 6.47E-4
20 SGTC_2156 5649887 3.16 115.0 µM 4.26E-4 RPP1 & pyrimidine depletion 42 7.79E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.336 6.20E-89 YML118W NGL3 hom 3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication
0.277 3.57E-60 YMR048W CSM3 hom Replication fork associated factor; required for stable replication fork pausing; component of the DNA replication checkpoint pathway; required for accurate chromosome segregation during meiosis; forms nuclear foci upon DNA replication stress
0.267 9.20E-56 YDL099W BUG1 hom Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes
0.242 8.31E-46 YOR037W CYC2 hom Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p)
0.237 4.50E-44 YDR505C PSP1 hom Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication
0.237 4.17E-44 YDL059C RAD59 hom Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p
0.232 3.14E-42 YBL085W BOI1 hom Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication
0.220 3.59E-38 YGL040C HEM2 het Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus
0.217 3.83E-37 YGL222C EDC1 hom RNA-binding protein that activates mRNA decapping directly; binds to the mRNA substrate and enhances the activity of the decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress
0.210 9.17E-35 YGR035C_p YGR035C_p hom Putative protein of unknown function, potential Cdc28p substrate; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance
0.205 4.19E-33 YGL205W POX1 hom Fatty-acyl coenzyme A oxidase, involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix
0.189 2.75E-28 YDR386W MUS81 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.187 9.10E-28 YBR067C TIP1 hom Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins
0.186 2.44E-27 YNR033W ABZ1 hom Para-aminobenzoate (PABA) synthase; has similarity to Escherichia coli PABA synthase components PabA and PabB; required for the synthesis of para-aminobenzoic acid, an important intermediate for folate and ubiquinone Q biosynthesis; protein abundance increases in response to DNA replication stress
0.183 1.12E-26 YLL032C YLL032C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene