YNR015W / SMM1

Dihydrouridine synthase, member of a family of dihydrouridine synthases including Dus1p, Smm1p, Dus3p, and Dus4p; modifies uridine residues at position 20 of cytoplasmic tRNAs

Zygosity: Homozygous strain
fixedexpanded
Profile for YNR015W / SMM1

Click on Significant Values for Screen Details ID:SGTC_150|Compound:3970-0969|FD-Score:2.82|P-value:8.09E-4 ID:SGTC_275|Compound:trichlorophene|FD-Score:3.47|P-value:5.24E-5 ID:SGTC_285|Compound:1486-1325|FD-Score:3.17|P-value:1.95E-4 ID:SGTC_286|Compound:k060-0067|FD-Score:3.21|P-value:1.64E-4 ID:SGTC_365|Compound:0121-0052|FD-Score:3.34|P-value:9.35E-5 ID:SGTC_372|Compound:0155-0182|FD-Score:2.85|P-value:7.14E-4 ID:SGTC_376|Compound:3970-0862|FD-Score:3.49|P-value:4.91E-5 ID:SGTC_383|Compound:2324-0395|FD-Score:2.85|P-value:7.12E-4 ID:SGTC_387|Compound:norethindrone acetate|FD-Score:2.91|P-value:5.69E-4 ID:SGTC_480|Compound:am 92016|FD-Score:-3.45|P-value:6.95E-5 ID:SGTC_506|Compound:n9-isopropylolomoucine|FD-Score:2.95|P-value:4.90E-4 ID:SGTC_547|Compound:1120-0019|FD-Score:3.24|P-value:1.44E-4 ID:SGTC_620|Compound:0109-0052|FD-Score:3.06|P-value:3.13E-4 ID:SGTC_655|Compound:k060-0012|FD-Score:-3.13|P-value:2.70E-4 ID:SGTC_667|Compound:1498-1044|FD-Score:-5.14|P-value:6.50E-9 ID:SGTC_708|Compound:3448-5400|FD-Score:-3.07|P-value:3.57E-4 ID:SGTC_927|Compound:2425-1185|FD-Score:3.48|P-value:5.04E-5 ID:SGTC_939|Compound:1165-0479|FD-Score:3.22|P-value:1.62E-4 ID:SGTC_1016|Compound:4226-1401|FD-Score:-3.49|P-value:5.71E-5 ID:SGTC_1023|Compound:4482-3888|FD-Score:3.19|P-value:1.85E-4 ID:SGTC_1040|Compound:k898-0338|FD-Score:2.94|P-value:5.14E-4 ID:SGTC_1187|Compound:1391-0724|FD-Score:-3.15|P-value:2.51E-4 ID:SGTC_1190|Compound:1486-1332|FD-Score:-3.25|P-value:1.68E-4 ID:SGTC_1240|Compound:0356-0016|FD-Score:3.32|P-value:1.03E-4 ID:SGTC_1271|Compound:0832-2808|FD-Score:3.09|P-value:2.72E-4 ID:SGTC_1283|Compound:0922-0125|FD-Score:2.77|P-value:9.74E-4 ID:SGTC_1379|Compound:2144-0755|FD-Score:2.83|P-value:7.64E-4 ID:SGTC_1398|Compound:3558-0053|FD-Score:3.22|P-value:1.59E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-4.15|P-value:2.28E-6 ID:SGTC_1491|Compound:4138-0063|FD-Score:3.43|P-value:6.44E-5 ID:SGTC_1499|Compound:4480-0877|FD-Score:3.09|P-value:2.79E-4 ID:SGTC_1518|Compound:st038567|FD-Score:-4.06|P-value:3.63E-6 ID:SGTC_1536|Compound:cytidine-2',3'-monophosphoric acid;  3'-cytidylic acid|FD-Score:-2.87|P-value:7.70E-4 ID:SGTC_1553|Compound:gossypol|FD-Score:3.22|P-value:1.59E-4 ID:SGTC_1595|Compound:6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin|FD-Score:3.10|P-value:2.61E-4 ID:SGTC_2017|Compound:5132927|FD-Score:-3.05|P-value:3.87E-4 ID:SGTC_2050|Compound:5263056|FD-Score:3.05|P-value:3.30E-4 ID:SGTC_2054|Compound:5233173|FD-Score:4.09|P-value:2.49E-6 ID:SGTC_2249|Compound:haloperidol|FD-Score:4.28|P-value:8.76E-7 ID:SGTC_2266|Compound:7929956|FD-Score:-3.29|P-value:1.38E-4 ID:SGTC_2293|Compound:7624732|FD-Score:-3.44|P-value:7.36E-5 ID:SGTC_2322|Compound:7413319|FD-Score:3.43|P-value:6.36E-5 ID:SGTC_2332|Compound:7982454|FD-Score:-3.15|P-value:2.55E-4 ID:SGTC_2347|Compound:9049783|FD-Score:3.68|P-value:1.94E-5 ID:SGTC_2449|Compound:5812982|FD-Score:-3.06|P-value:3.67E-4 ID:SGTC_2788|Compound:5282734|FD-Score:5.29|P-value:1.80E-9 ID:SGTC_2790|Compound:5423970|FD-Score:3.07|P-value:3.05E-4 ID:SGTC_2896|Compound:9052656|FD-Score:3.12|P-value:2.39E-4 ID:SGTC_3062|Compound:9077494|FD-Score:3.21|P-value:1.70E-4 ID:SGTC_3321|Compound:9137793|FD-Score:-4.03|P-value:4.14E-6 ID:SGTC_3334|Compound:9142812|FD-Score:3.44|P-value:6.06E-5 ID:SGTC_150|Compound:3970-0969|FD-Score:2.82|P-value:8.09E-4 ID:SGTC_275|Compound:trichlorophene|FD-Score:3.47|P-value:5.24E-5 ID:SGTC_285|Compound:1486-1325|FD-Score:3.17|P-value:1.95E-4 ID:SGTC_286|Compound:k060-0067|FD-Score:3.21|P-value:1.64E-4 ID:SGTC_365|Compound:0121-0052|FD-Score:3.34|P-value:9.35E-5 ID:SGTC_372|Compound:0155-0182|FD-Score:2.85|P-value:7.14E-4 ID:SGTC_376|Compound:3970-0862|FD-Score:3.49|P-value:4.91E-5 ID:SGTC_383|Compound:2324-0395|FD-Score:2.85|P-value:7.12E-4 ID:SGTC_387|Compound:norethindrone acetate|FD-Score:2.91|P-value:5.69E-4 ID:SGTC_480|Compound:am 92016|FD-Score:-3.45|P-value:6.95E-5 ID:SGTC_506|Compound:n9-isopropylolomoucine|FD-Score:2.95|P-value:4.90E-4 ID:SGTC_547|Compound:1120-0019|FD-Score:3.24|P-value:1.44E-4 ID:SGTC_620|Compound:0109-0052|FD-Score:3.06|P-value:3.13E-4 ID:SGTC_655|Compound:k060-0012|FD-Score:-3.13|P-value:2.70E-4 ID:SGTC_667|Compound:1498-1044|FD-Score:-5.14|P-value:6.50E-9 ID:SGTC_708|Compound:3448-5400|FD-Score:-3.07|P-value:3.57E-4 ID:SGTC_927|Compound:2425-1185|FD-Score:3.48|P-value:5.04E-5 ID:SGTC_939|Compound:1165-0479|FD-Score:3.22|P-value:1.62E-4 ID:SGTC_1016|Compound:4226-1401|FD-Score:-3.49|P-value:5.71E-5 ID:SGTC_1023|Compound:4482-3888|FD-Score:3.19|P-value:1.85E-4 ID:SGTC_1040|Compound:k898-0338|FD-Score:2.94|P-value:5.14E-4 ID:SGTC_1187|Compound:1391-0724|FD-Score:-3.15|P-value:2.51E-4 ID:SGTC_1190|Compound:1486-1332|FD-Score:-3.25|P-value:1.68E-4 ID:SGTC_1240|Compound:0356-0016|FD-Score:3.32|P-value:1.03E-4 ID:SGTC_1271|Compound:0832-2808|FD-Score:3.09|P-value:2.72E-4 ID:SGTC_1283|Compound:0922-0125|FD-Score:2.77|P-value:9.74E-4 ID:SGTC_1379|Compound:2144-0755|FD-Score:2.83|P-value:7.64E-4 ID:SGTC_1398|Compound:3558-0053|FD-Score:3.22|P-value:1.59E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-4.15|P-value:2.28E-6 ID:SGTC_1491|Compound:4138-0063|FD-Score:3.43|P-value:6.44E-5 ID:SGTC_1499|Compound:4480-0877|FD-Score:3.09|P-value:2.79E-4 ID:SGTC_1518|Compound:st038567|FD-Score:-4.06|P-value:3.63E-6 ID:SGTC_1536|Compound:cytidine-2',3'-monophosphoric acid;  3'-cytidylic acid|FD-Score:-2.87|P-value:7.70E-4 ID:SGTC_1553|Compound:gossypol|FD-Score:3.22|P-value:1.59E-4 ID:SGTC_1595|Compound:6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin|FD-Score:3.10|P-value:2.61E-4 ID:SGTC_2017|Compound:5132927|FD-Score:-3.05|P-value:3.87E-4 ID:SGTC_2050|Compound:5263056|FD-Score:3.05|P-value:3.30E-4 ID:SGTC_2054|Compound:5233173|FD-Score:4.09|P-value:2.49E-6 ID:SGTC_2249|Compound:haloperidol|FD-Score:4.28|P-value:8.76E-7 ID:SGTC_2266|Compound:7929956|FD-Score:-3.29|P-value:1.38E-4 ID:SGTC_2293|Compound:7624732|FD-Score:-3.44|P-value:7.36E-5 ID:SGTC_2322|Compound:7413319|FD-Score:3.43|P-value:6.36E-5 ID:SGTC_2332|Compound:7982454|FD-Score:-3.15|P-value:2.55E-4 ID:SGTC_2347|Compound:9049783|FD-Score:3.68|P-value:1.94E-5 ID:SGTC_2449|Compound:5812982|FD-Score:-3.06|P-value:3.67E-4 ID:SGTC_2788|Compound:5282734|FD-Score:5.29|P-value:1.80E-9 ID:SGTC_2790|Compound:5423970|FD-Score:3.07|P-value:3.05E-4 ID:SGTC_2896|Compound:9052656|FD-Score:3.12|P-value:2.39E-4 ID:SGTC_3062|Compound:9077494|FD-Score:3.21|P-value:1.70E-4 ID:SGTC_3321|Compound:9137793|FD-Score:-4.03|P-value:4.14E-6 ID:SGTC_3334|Compound:9142812|FD-Score:3.44|P-value:6.06E-5

Top fitness defect scores for YNR015W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2788 5282734 5.29 71.4 µM 1.80E-9 20 6.01E-8
2 SGTC_2249 haloperidol 4.28 116.4 µM 8.76E-7 ergosterol depletion effects on membrane 91 9.18E-6
3 SGTC_2054 5233173 4.09 129.0 µM 2.49E-6 mitochondrial response to ROS 50 2.15E-5
4 SGTC_2347 9049783 3.68 174.8 µM 1.94E-5 41 1.14E-4
5 SGTC_376 3970-0862 3.49 59.1 µM 4.91E-5 34 2.44E-4
6 SGTC_927 2425-1185 3.48 123.0 µM 5.04E-5 45 2.50E-4
7 SGTC_275 trichlorophene 3.47 5.7 µM 5.24E-5 mitochondrial stress 258 2.58E-4
8 SGTC_3334 9142812 3.44 21.9 µM 6.06E-5 44 2.90E-4
9 SGTC_2322 7413319 3.43 38.2 µM 6.36E-5 ERAD & cell cycle 193 3.01E-4
10 SGTC_1491 4138-0063 3.43 26.5 µM 6.44E-5 18 3.04E-4
11 SGTC_365 0121-0052 3.34 13.6 µM 9.35E-5 mitochondrial response to ROS 93 4.13E-4
12 SGTC_1240 0356-0016 3.32 42.1 µM 1.03E-4 mitochondrial response to ROS 58 4.47E-4
13 SGTC_547 1120-0019 3.24 610.0 nM 1.44E-4 azole & statin 54 5.88E-4
14 SGTC_1553 gossypol 3.22 38.6 µM 1.59E-4 6 6.36E-4
15 SGTC_1398 3558-0053 3.22 112.0 µM 1.59E-4 11 6.37E-4
16 SGTC_939 1165-0479 3.22 45.3 µM 1.62E-4 69 6.47E-4
17 SGTC_286 k060-0067 3.21 22.9 µM 1.64E-4 tubulin folding & SWR complex 43 6.53E-4
18 SGTC_3062 9077494 3.21 49.5 µM 1.70E-4 mitochondrial response to ROS 62 6.72E-4
19 SGTC_1023 4482-3888 3.19 16.0 µM 1.85E-4 TSC3-RPN4 46 7.21E-4
20 SGTC_285 1486-1325 3.17 46.0 µM 1.95E-4 44 7.54E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.234 4.15E-43 YFL036W RPO41 hom Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation
0.206 2.08E-33 YOR101W RAS1 hom GTPase involved in G-protein signaling in adenylate cyclase activation; plays a role in cell proliferation; localized to the plasma membrane; homolog of mammalian RAS proto-oncogenes; relative distribution to the nucleus increases upon DNA replication stress
0.169 6.05E-23 YJL171C YJL171C hom GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress
0.126 2.63E-13 YKL139W CTK1 hom Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13
0.123 8.78E-13 YBR078W ECM33 hom GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p
0.122 1.21E-12 YJL078C PRY3 hom Cell wall-associated protein involved in export of acetylated sterols; member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); role in mating efficiency; expression of full-length transcript is daughter cell-specific; in response to alpha factor, a short transcript starting at +452 is expressed and the long form is repressed by Ste12p
0.119 5.20E-12 YDR371W_p CTS2_p hom Putative chitinase, functionally complements A. gossypii cts2 mutant sporulation defect
0.107 5.85E-10 YJR058C APS2 hom Small subunit of the clathrin-associated adaptor complex AP-2, which is involved in protein sorting at the plasma membrane; related to the sigma subunit of the mammalian plasma membrane clathrin-associated protein (AP-2) complex
0.103 1.87E-9 YGR022C_d YGR022C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W
0.100 6.50E-9 YDR513W GRX2 hom Cytoplasmic glutaredoxin; thioltransferase, glutathione-dependent disulfide oxidoreductase involved in maintaining redox state of target proteins, also exhibits glutathione peroxidase activity, expression induced in response to stress; GRX2 has a paralog, GRX1, that arose from the whole genome duplication
0.099 9.51E-9 YKL128C PMU1 hom Putative phosphomutase, contains a region homologous to the active site of phosphomutases; overexpression suppresses the histidine auxotrophy of an ade3 ade16 ade17 triple mutant and the temperature sensitivity of a tps2 mutant
0.099 1.01E-8 YER046W SPO73 hom Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensible for both nuclear divisions during meiosis
0.097 1.62E-8 YDR517W GRH1 hom Acetylated cis-Golgi protein, involved in ER to Golgi transport; homolog of human GRASP65; forms a complex with the coiled-coil protein Bug1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes; protein abundance increases in response to DNA replication stress
0.097 1.86E-8 YNL044W YIP3 hom Protein localized to COPII vesicles, proposed to be involved in ER to Golgi transport; interacts with members of the Rab GTPase family and Yip1p; also interacts with Rtn1p
0.096 2.18E-8 YLR020C YEH2 hom Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism