YOL007C / CSI2

Protein of unknown function; green fluorescent protein (GFP)- fusion protein localizes to the mother side of the bud neck and the vacuole; YOL007C is not an essential gene

Zygosity: Homozygous strain
fixedexpanded
Profile for YOL007C / CSI2

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:4.18|P-value:9.42E-6 ID:SGTC_69|Compound:0096-0274|FD-Score:3.13|P-value:6.83E-4 ID:SGTC_161|Compound:4065-0411|FD-Score:-3.53|P-value:1.65E-4 ID:SGTC_204|Compound:1543-0184|FD-Score:-3.19|P-value:5.86E-4 ID:SGTC_313|Compound:3474-0040|FD-Score:3.29|P-value:3.81E-4 ID:SGTC_734|Compound:2023-0417|FD-Score:3.89|P-value:3.55E-5 ID:SGTC_1228|Compound:0124-0032|FD-Score:3.68|P-value:8.33E-5 ID:SGTC_1239|Compound:0337-0584|FD-Score:3.27|P-value:4.06E-4 ID:SGTC_1325|Compound:1431-0015|FD-Score:6.32|P-value:5.43E-11 ID:SGTC_1333|Compound:1482-0047|FD-Score:-4.25|P-value:7.81E-6 ID:SGTC_1367|Compound:1812-0510|FD-Score:3.16|P-value:6.01E-4 ID:SGTC_1425|Compound:4048-4433|FD-Score:3.89|P-value:3.43E-5 ID:SGTC_1556|Compound:plumbagin|FD-Score:3.54|P-value:1.45E-4 ID:SGTC_1629|Compound:st005085|FD-Score:4.34|P-value:4.60E-6 ID:SGTC_1631|Compound:benzylvanillin|FD-Score:-3.24|P-value:5.05E-4 ID:SGTC_1664|Compound:st014217|FD-Score:3.28|P-value:4.00E-4 ID:SGTC_1791|Compound:st048313|FD-Score:-3.54|P-value:1.59E-4 ID:SGTC_1799|Compound:st045822|FD-Score:-3.22|P-value:5.38E-4 ID:SGTC_2079|Compound:5212404|FD-Score:3.14|P-value:6.62E-4 ID:SGTC_2128|Compound:5255449|FD-Score:3.79|P-value:5.37E-5 ID:SGTC_2134|Compound:5324836|FD-Score:4.93|P-value:2.40E-7 ID:SGTC_2135|Compound:5326629|FD-Score:3.63|P-value:1.01E-4 ID:SGTC_2243|Compound:6634756|FD-Score:3.42|P-value:2.34E-4 ID:SGTC_2284|Compound:7945075|FD-Score:-3.73|P-value:7.62E-5 ID:SGTC_2345|Compound:9052315|FD-Score:3.21|P-value:5.07E-4 ID:SGTC_2430|Compound:5571206|FD-Score:-3.05|P-value:9.62E-4 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:4.19|P-value:9.21E-6 ID:SGTC_2908|Compound:7930301|FD-Score:-3.78|P-value:6.09E-5 ID:SGTC_2942|Compound:9052777|FD-Score:3.03|P-value:9.76E-4 ID:SGTC_3204|Compound:9114183|FD-Score:3.32|P-value:3.40E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:4.18|P-value:9.42E-6 ID:SGTC_69|Compound:0096-0274|FD-Score:3.13|P-value:6.83E-4 ID:SGTC_161|Compound:4065-0411|FD-Score:-3.53|P-value:1.65E-4 ID:SGTC_204|Compound:1543-0184|FD-Score:-3.19|P-value:5.86E-4 ID:SGTC_313|Compound:3474-0040|FD-Score:3.29|P-value:3.81E-4 ID:SGTC_734|Compound:2023-0417|FD-Score:3.89|P-value:3.55E-5 ID:SGTC_1228|Compound:0124-0032|FD-Score:3.68|P-value:8.33E-5 ID:SGTC_1239|Compound:0337-0584|FD-Score:3.27|P-value:4.06E-4 ID:SGTC_1325|Compound:1431-0015|FD-Score:6.32|P-value:5.43E-11 ID:SGTC_1333|Compound:1482-0047|FD-Score:-4.25|P-value:7.81E-6 ID:SGTC_1367|Compound:1812-0510|FD-Score:3.16|P-value:6.01E-4 ID:SGTC_1425|Compound:4048-4433|FD-Score:3.89|P-value:3.43E-5 ID:SGTC_1556|Compound:plumbagin|FD-Score:3.54|P-value:1.45E-4 ID:SGTC_1629|Compound:st005085|FD-Score:4.34|P-value:4.60E-6 ID:SGTC_1631|Compound:benzylvanillin|FD-Score:-3.24|P-value:5.05E-4 ID:SGTC_1664|Compound:st014217|FD-Score:3.28|P-value:4.00E-4 ID:SGTC_1791|Compound:st048313|FD-Score:-3.54|P-value:1.59E-4 ID:SGTC_1799|Compound:st045822|FD-Score:-3.22|P-value:5.38E-4 ID:SGTC_2079|Compound:5212404|FD-Score:3.14|P-value:6.62E-4 ID:SGTC_2128|Compound:5255449|FD-Score:3.79|P-value:5.37E-5 ID:SGTC_2134|Compound:5324836|FD-Score:4.93|P-value:2.40E-7 ID:SGTC_2135|Compound:5326629|FD-Score:3.63|P-value:1.01E-4 ID:SGTC_2243|Compound:6634756|FD-Score:3.42|P-value:2.34E-4 ID:SGTC_2284|Compound:7945075|FD-Score:-3.73|P-value:7.62E-5 ID:SGTC_2345|Compound:9052315|FD-Score:3.21|P-value:5.07E-4 ID:SGTC_2430|Compound:5571206|FD-Score:-3.05|P-value:9.62E-4 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:4.19|P-value:9.21E-6 ID:SGTC_2908|Compound:7930301|FD-Score:-3.78|P-value:6.09E-5 ID:SGTC_2942|Compound:9052777|FD-Score:3.03|P-value:9.76E-4 ID:SGTC_3204|Compound:9114183|FD-Score:3.32|P-value:3.40E-4

Top fitness defect scores for YOL007C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1325 1431-0015 6.32 227.0 µM 5.43E-11 Golgi 2 1.32E-10
2 SGTC_2134 5324836 4.93 1.5 µM 2.40E-7 5 4.19E-7
3 SGTC_1629 st005085 4.34 44.9 µM 4.60E-6 RPP1 & pyrimidine depletion 25 7.17E-6
4 SGTC_2637 3-alpha-acetoxydihydrodeoxygedunin 4.19 100.0 µM 9.21E-6 21 1.40E-5
5 SGTC_6 0844-0013 4.18 19.8 µM 9.42E-6 71 1.43E-5
6 SGTC_1425 4048-4433 3.89 7.3 µM 3.43E-5 copper-dependent oxidative stress 42 4.94E-5
7 SGTC_734 2023-0417 3.89 80.0 µM 3.55E-5 ergosterol biosynthesis 18 5.11E-5
8 SGTC_2128 5255449 3.79 168.5 µM 5.37E-5 40 7.61E-5
9 SGTC_1228 0124-0032 3.68 52.5 µM 8.33E-5 25 1.16E-4
10 SGTC_2135 5326629 3.63 150.4 µM 1.01E-4 RPP1 & pyrimidine depletion 38 1.39E-4
11 SGTC_1556 plumbagin 3.54 1.5 µM 1.45E-4 superoxide 27 1.98E-4
12 SGTC_2243 6634756 3.42 200.0 µM 2.34E-4 36 3.13E-4
13 SGTC_3204 9114183 3.32 49.5 µM 3.40E-4 57 4.49E-4
14 SGTC_313 3474-0040 3.29 5.0 µM 3.81E-4 NEO1-PIK1 105 5.01E-4
15 SGTC_1664 st014217 3.28 9.0 µM 4.00E-4 60S ribosome export 27 5.24E-4
16 SGTC_1239 0337-0584 3.27 35.4 µM 4.06E-4 unfolded protein response 91 5.32E-4
17 SGTC_2345 9052315 3.21 157.4 µM 5.07E-4 51 6.59E-4
18 SGTC_1367 1812-0510 3.16 76.9 µM 6.01E-4 59 7.76E-4
19 SGTC_2079 5212404 3.14 121.0 µM 6.62E-4 30 8.51E-4
20 SGTC_69 0096-0274 3.13 206.7 µM 6.83E-4 mitochondrial processes 96 8.77E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.122 1.27E-12 YPR022C_p YPR022C_p hom Putative transcription factor, as suggested by computational analysis; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS
0.117 1.00E-11 YML130C ERO1 het Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds
0.112 7.00E-11 YDR530C APA2 hom Diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase II; AP4A phosphorylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; APA2 has a paralog, APA1, that arose from the whole genome duplication
0.112 8.33E-11 YNL152W INN1 het Essential protein that associates with contractile actomyosin ring; required for ingression of the plasma membrane into the bud neck during cytokinesis; C2 domain, a membrane targeting module, is required for function; activates chitin synthase activity of Chs2p during cytokinesis
0.103 2.41E-9 YLR329W REC102 hom Protein involved in early stages of meiotic recombination; required for chromosome synapsis; forms a complex with Rec104p and Spo11p necessary during the initiation of recombination
0.103 2.55E-9 YFL032W_d YFL032W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene
0.094 4.51E-8 YBL030C PET9 het Major ADP/ATP carrier of the mitochondrial inner membrane; exchanges cytosolic ADP for mitochondrially synthesized ATP; also imports heme and ATP; phosphorylated; required for viability in many lab strains that carry a sal1 mutation; PET9 has a paralog, AAC3, that arose from the whole genome duplication
0.091 1.35E-7 YJL033W HCA4 het DEAD box RNA helicase; high-copy number suppression of a U14 snoRNA processing mutant suggests an involvement in 18S rRNA synthesis
0.088 3.48E-7 YOR352W TFB6 hom Subunit of TFIIH complex; facilities dissociation of the Ssl2p helices from TFIIH; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
0.086 6.21E-7 YJR008W MHO1 hom Protein of unknown function; inhibits haploid invasive growth when overexpressed; synthetically lethal with phospholipase C (PLC1); expression induced by mild heat-stress on a non-fermentable carbon source, upon entry into stationary phase and upon nitrogen deprivation; repressed by inosine and choline in an Opi1p-dependent manner; highly conserved from bacteria to human; Memo, the human homolog, is an ErbB2 interacting protein with an essential function in cell motility
0.086 6.43E-7 YMR190C SGS1 hom Nucleolar DNA helicase of the RecQ family; involved in genome integrity maintenance; regulates chromosome synapsis and meiotic joint molecule/crossover formation; potential role as repressor of a subset of rapamycin responsive genes; rapidly lost in response to rapamycin in Rrd1p-dependent manner; similar to human BLM and WRN proteins implicated in Bloom and Werner syndromes; forms nuclear foci upon DNA replication stress
0.086 7.11E-7 YJL034W KAR2 het ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p
0.083 1.65E-6 YCL075W YCL075W hom Pseudogene: encodes fragment of Ty Pol protein
0.080 3.23E-6 YPR074C TKL1 hom Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication
0.079 4.87E-6 YMR172W HOT1 hom Transcription factor required for the transient induction of glycerol biosynthetic genes GPD1 and GPP2 in response to high osmolarity; targets Hog1p to osmostress responsive promoters; has similarity to Msn1p and Gcr1p