HIPHOP chemogenomics database

YOL012C / HTZ1

Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin

Zygosity: Homozygous strain

fixedexpanded
Profile for YOL012C / HTZ1

Click on Significant Values for Screen Details

Top fitness defect scores for YOL012C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_897	1000-0139	8.69	31.4 µM	4.95E-16		8	1.81E-18
2	SGTC_229	benomyl	8.36	22.9 µM	5.76E-15	tubulin folding & SWR complex	13	3.16E-17
3	SGTC_351	caspofungin	8.13	25.0 nM	2.90E-14	cell wall	16	2.08E-16
4	SGTC_2174	5790901	7.57	200.0 µM	1.40E-12	tubulin folding & SWR complex	13	1.92E-14
5	SGTC_86	0417-1630	7.33	471.9 µM	6.57E-12	ubiquinone biosynthesis & proteasome	66	1.16E-13
6	SGTC_2200	6600604	7.33	21.9 µM	6.69E-12	tubulin folding & SWR complex	19	1.19E-13
7	SGTC_2489	cantharidin	6.71	73.4 µM	2.90E-10	cell wall signaling	5	9.62E-12
8	SGTC_2782	7736369	6.38	71.4 µM	1.97E-9		4	8.97E-11
9	SGTC_2502	avocadyne acetate	6.09	18.6 µM	9.56E-9		7	5.66E-10
10	SGTC_212	3807-4682	6.01	38.8 µM	1.46E-8	tubulin folding & SWR complex	27	9.26E-10
11	SGTC_771	0250-0039	5.93	27.3 µM	2.24E-8		5	1.53E-9
12	SGTC_2233	sulbentine	5.85	40.4 µM	3.43E-8		26	2.51E-9
13	SGTC_436	n,n-dimethylsphingosine	5.83	3.3 µM	3.80E-8		3	2.83E-9
14	SGTC_210	3551-0445	5.54	56.8 µM	1.63E-7		29	1.55E-8
15	SGTC_367	0335-0881	5.53	7.1 µM	1.69E-7	ubiquinone biosynthesis & proteasome	28	1.61E-8
16	SGTC_445	e6 berbamine	5.51	66.0 µM	1.89E-7	NEO1-PIK1	41	1.83E-8
17	SGTC_221	trifluoperazine	5.47	10.3 µM	2.30E-7	NEO1-PIK1	146	2.31E-8
18	SGTC_2573	2',4'-dihydroxychalcone	5.42	15.0 µM	2.93E-7		20	3.05E-8
19	SGTC_1013	4112-3890	5.36	241.0 µM	3.73E-7	TSC3-RPN4	11	4.05E-8
20	SGTC_912	3474-0017	5.33	2.2 µM	4.38E-7		7	4.87E-8

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.471	4.74E-185	YLR085C	ARP6	hom	Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A
0.446	1.47E-163	YDR485C	VPS72	hom	Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting
0.416	1.56E-140	YDR334W	SWR1	hom	Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A
0.415	1.38E-139	YAL011W	SWC3	hom	Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae
0.408	2.13E-134	YML041C	VPS71	hom	Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting
0.387	1.86E-120	YBR231C	SWC5	hom	Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress
0.360	2.49E-103	YML124C	TUB3	hom	Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication
0.357	3.58E-101	YER007W	PAC2	hom	Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl
0.288	5.39E-65	YOR349W	CIN1	hom	Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl
0.254	1.85E-50	YPL241C	CIN2	hom	GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2
0.186	1.73E-27	YOR254C	SEC63	het	Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
0.180	7.86E-26	YJL168C	SET2	hom	Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36, which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p
0.179	1.75E-25	YMR110C	HFD1	hom	Hexadecenal dehydrogenase; involved in the conversion of sphingosine 1-phosphate breakdown product hexadecenal to hexadecenoic acid; located in the mitochondrial outer membrane and also in lipid particles; has similarity to ALDH3A2, a human fatty aldehyde dehydrogenase (FALDH) mutated in Sjogren-Larsson syndrome, a neurocutaneous disorder
0.171	1.99E-23	YJL169W_d	YJL169W_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2
0.170	4.78E-23	YML034C-A_d	YML034C-A_d	hom	Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data