YOL049W / GSH2

Glutathione synthetase, catalyzes the ATP-dependent synthesis of glutathione (GSH) from gamma-glutamylcysteine and glycine; induced by oxidative stress and heat shock

Zygosity: Homozygous strain
fixedexpanded
Profile for YOL049W / GSH2

Click on Significant Values for Screen Details ID:SGTC_7|Compound:0192-1582|FD-Score:3.68|P-value:1.34E-4 ID:SGTC_81|Compound:0411-0046|FD-Score:5.02|P-value:3.58E-7 ID:SGTC_107|Compound:0358-0022|FD-Score:3.95|P-value:4.70E-5 ID:SGTC_114|Compound:0364-0001|FD-Score:3.37|P-value:4.16E-4 ID:SGTC_150|Compound:3970-0969|FD-Score:6.00|P-value:1.62E-9 ID:SGTC_159|Compound:3970-0959|FD-Score:4.62|P-value:2.44E-6 ID:SGTC_163|Compound:1327-0036|FD-Score:3.26|P-value:6.21E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:9.45|P-value:6.15E-21 ID:SGTC_310|Compound:1273-0058|FD-Score:3.50|P-value:2.65E-4 ID:SGTC_398|Compound:bronopol|FD-Score:3.16|P-value:8.56E-4 ID:SGTC_666|Compound:0272-0154|FD-Score:-4.10|P-value:3.18E-5 ID:SGTC_852|Compound:0418-0143|FD-Score:3.28|P-value:5.68E-4 ID:SGTC_966|Compound:1300-0360|FD-Score:3.28|P-value:5.84E-4 ID:SGTC_993|Compound:3909-8757|FD-Score:-4.55|P-value:4.36E-6 ID:SGTC_1001|Compound:4048-4355|FD-Score:4.45|P-value:5.52E-6 ID:SGTC_1063|Compound:disulfiram|FD-Score:3.34|P-value:4.75E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:-4.33|P-value:1.20E-5 ID:SGTC_1101|Compound:ebselen|FD-Score:4.53|P-value:3.77E-6 ID:SGTC_1115|Compound:galangin|FD-Score:3.84|P-value:7.28E-5 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.45|P-value:3.88E-4 ID:SGTC_1695|Compound:st026914|FD-Score:3.26|P-value:6.09E-4 ID:SGTC_1704|Compound:st028805|FD-Score:4.10|P-value:2.53E-5 ID:SGTC_1939|Compound:st074455|FD-Score:3.18|P-value:7.97E-4 ID:SGTC_1950|Compound:st077106|FD-Score:4.22|P-value:1.46E-5 ID:SGTC_2001|Compound:4023502|FD-Score:-3.23|P-value:8.19E-4 ID:SGTC_2208|Compound:7283366|FD-Score:4.82|P-value:9.26E-7 ID:SGTC_2343|Compound:7990716|FD-Score:-3.44|P-value:3.95E-4 ID:SGTC_2473|Compound:5854593|FD-Score:3.30|P-value:5.43E-4 ID:SGTC_2498|Compound:4'-Methoxychalcone|FD-Score:5.12|P-value:2.12E-7 ID:SGTC_2592|Compound:piperlongumine|FD-Score:-3.43|P-value:4.08E-4 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:4.06|P-value:2.98E-5 ID:SGTC_2653|Compound:dihydrofissinolide|FD-Score:-4.17|P-value:2.31E-5 ID:SGTC_2681|Compound:menadione|FD-Score:4.26|P-value:1.26E-5 ID:SGTC_2701|Compound:st077767|FD-Score:3.81|P-value:8.19E-5 ID:SGTC_2705|Compound:st078069|FD-Score:3.12|P-value:9.79E-4 ID:SGTC_2970|Compound:9090938|FD-Score:5.28|P-value:9.25E-8 ID:SGTC_2996|Compound:9066192|FD-Score:4.63|P-value:2.36E-6 ID:SGTC_3129|Compound:9127257|FD-Score:3.17|P-value:8.46E-4 ID:SGTC_3134|Compound:9127454|FD-Score:-3.54|P-value:2.76E-4 ID:SGTC_3238|Compound:9133470|FD-Score:3.73|P-value:1.13E-4 ID:SGTC_3350|Compound:9150499|FD-Score:3.72|P-value:1.16E-4 ID:SGTC_7|Compound:0192-1582|FD-Score:3.68|P-value:1.34E-4 ID:SGTC_81|Compound:0411-0046|FD-Score:5.02|P-value:3.58E-7 ID:SGTC_107|Compound:0358-0022|FD-Score:3.95|P-value:4.70E-5 ID:SGTC_114|Compound:0364-0001|FD-Score:3.37|P-value:4.16E-4 ID:SGTC_150|Compound:3970-0969|FD-Score:6.00|P-value:1.62E-9 ID:SGTC_159|Compound:3970-0959|FD-Score:4.62|P-value:2.44E-6 ID:SGTC_163|Compound:1327-0036|FD-Score:3.26|P-value:6.21E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:9.45|P-value:6.15E-21 ID:SGTC_310|Compound:1273-0058|FD-Score:3.50|P-value:2.65E-4 ID:SGTC_398|Compound:bronopol|FD-Score:3.16|P-value:8.56E-4 ID:SGTC_666|Compound:0272-0154|FD-Score:-4.10|P-value:3.18E-5 ID:SGTC_852|Compound:0418-0143|FD-Score:3.28|P-value:5.68E-4 ID:SGTC_966|Compound:1300-0360|FD-Score:3.28|P-value:5.84E-4 ID:SGTC_993|Compound:3909-8757|FD-Score:-4.55|P-value:4.36E-6 ID:SGTC_1001|Compound:4048-4355|FD-Score:4.45|P-value:5.52E-6 ID:SGTC_1063|Compound:disulfiram|FD-Score:3.34|P-value:4.75E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:-4.33|P-value:1.20E-5 ID:SGTC_1101|Compound:ebselen|FD-Score:4.53|P-value:3.77E-6 ID:SGTC_1115|Compound:galangin|FD-Score:3.84|P-value:7.28E-5 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.45|P-value:3.88E-4 ID:SGTC_1695|Compound:st026914|FD-Score:3.26|P-value:6.09E-4 ID:SGTC_1704|Compound:st028805|FD-Score:4.10|P-value:2.53E-5 ID:SGTC_1939|Compound:st074455|FD-Score:3.18|P-value:7.97E-4 ID:SGTC_1950|Compound:st077106|FD-Score:4.22|P-value:1.46E-5 ID:SGTC_2001|Compound:4023502|FD-Score:-3.23|P-value:8.19E-4 ID:SGTC_2208|Compound:7283366|FD-Score:4.82|P-value:9.26E-7 ID:SGTC_2343|Compound:7990716|FD-Score:-3.44|P-value:3.95E-4 ID:SGTC_2473|Compound:5854593|FD-Score:3.30|P-value:5.43E-4 ID:SGTC_2498|Compound:4'-Methoxychalcone|FD-Score:5.12|P-value:2.12E-7 ID:SGTC_2592|Compound:piperlongumine|FD-Score:-3.43|P-value:4.08E-4 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:4.06|P-value:2.98E-5 ID:SGTC_2653|Compound:dihydrofissinolide|FD-Score:-4.17|P-value:2.31E-5 ID:SGTC_2681|Compound:menadione|FD-Score:4.26|P-value:1.26E-5 ID:SGTC_2701|Compound:st077767|FD-Score:3.81|P-value:8.19E-5 ID:SGTC_2705|Compound:st078069|FD-Score:3.12|P-value:9.79E-4 ID:SGTC_2970|Compound:9090938|FD-Score:5.28|P-value:9.25E-8 ID:SGTC_2996|Compound:9066192|FD-Score:4.63|P-value:2.36E-6 ID:SGTC_3129|Compound:9127257|FD-Score:3.17|P-value:8.46E-4 ID:SGTC_3134|Compound:9127454|FD-Score:-3.54|P-value:2.76E-4 ID:SGTC_3238|Compound:9133470|FD-Score:3.73|P-value:1.13E-4 ID:SGTC_3350|Compound:9150499|FD-Score:3.72|P-value:1.16E-4

Top fitness defect scores for YOL049W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_168 3970-0862 9.45 530.5 µM 6.15E-21 12 1.70E-21
2 SGTC_150 3970-0969 6.00 77.2 µM 1.62E-9 20 1.01E-9
3 SGTC_2970 9090938 5.28 41.9 µM 9.25E-8 RNA processing & uracil transport 9 6.54E-8
4 SGTC_2498 4'-Methoxychalcone 5.12 54.6 µM 2.12E-7 RPP1 & pyrimidine depletion 11 1.53E-7
5 SGTC_81 0411-0046 5.02 10.0 µM 3.58E-7 14 2.63E-7
6 SGTC_2208 7283366 4.82 200.0 µM 9.26E-7 4 7.00E-7
7 SGTC_2996 9066192 4.63 71.4 µM 2.36E-6 15 1.83E-6
8 SGTC_159 3970-0959 4.62 50.0 µM 2.44E-6 49 1.90E-6
9 SGTC_1101 ebselen 4.53 5.3 µM 3.77E-6 12 2.97E-6
10 SGTC_1001 4048-4355 4.45 44.4 µM 5.52E-6 copper-dependent oxidative stress 23 4.39E-6
11 SGTC_2681 menadione 4.26 3.2 µM 1.26E-5 superoxide 27 1.02E-5
12 SGTC_1950 st077106 4.22 7.2 µM 1.46E-5 RNA processing & uracil transport 22 1.20E-5
13 SGTC_1704 st028805 4.10 70.8 µM 2.53E-5 6 2.10E-5
14 SGTC_2603 homopterocarpin 4.06 100.0 µM 2.98E-5 34 2.48E-5
15 SGTC_107 0358-0022 3.95 2.7 µM 4.70E-5 SWF1 & branched chain AA biosynthesis 27 3.96E-5
16 SGTC_1115 galangin 3.84 695.7 nM 7.28E-5 RPP1 & pyrimidine depletion 17 6.22E-5
17 SGTC_2701 st077767 3.81 14.7 µM 8.19E-5 45 7.01E-5
18 SGTC_3238 9133470 3.73 49.5 µM 1.13E-4 16 9.77E-5
19 SGTC_3350 9150499 3.72 3.6 µM 1.16E-4 cell wall 65 1.00E-4
20 SGTC_7 0192-1582 3.68 4.2 µM 1.34E-4 73 1.16E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.307 3.41E-74 YJL101C GSH1 hom Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress
0.219 1.31E-37 YDR005C MAF1 hom Highly conserved negative regulator of RNA polymerase III; binds to the N-terminal domain of the Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to the nucleus and nucleolus under stress conditions
0.204 1.01E-32 YFL033C RIM15 hom Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase
0.177 6.07E-25 YIR012W SQT1 het Protein involved in 60S ribosomal subunit assembly or modification; contains multiple WD repeats; interacts with Qsr1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress
0.171 1.77E-23 YDR049W VMS1 hom Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans
0.165 6.91E-22 YDR069C DOA4 hom Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole
0.156 9.54E-20 YJL202C_d YJL202C_d het Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 3' end of essential PRP21 gene encoding a subunit of the SF3a splicing factor complex
0.155 1.69E-19 YHR205W SCH9 hom AGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan
0.154 2.58E-19 YDR002W YRB1 het Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1
0.152 7.82E-19 YJR074W MOG1 hom Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p
0.149 3.92E-18 YJR013W GPI14 het Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M
0.148 6.04E-18 YCR053W THR4 hom Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway
0.143 9.76E-17 YKL193C SDS22 het Regulatory subunit of the type 1 protein phosphatase (PP1) Glc7p; whether it functions as a positive or negative regulator of Glc7p is controversial; involved in the regulation of Glc7p nuclear localization and function
0.140 4.19E-16 YJR139C HOM6 hom Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions
0.138 7.98E-16 YDR017C KCS1 hom Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; generation of high energy inositol pyrophosphates by Kcs1p is required for many processes such as vacuolar biogenesis, stress response and telomere maintenance