YOL058W / ARG1

Arginosuccinate synthetase, catalyzes the formation of L-argininosuccinate from citrulline and L-aspartate in the arginine biosynthesis pathway; potential Cdc28p substrate

Zygosity: Homozygous strain
fixedexpanded
Profile for YOL058W / ARG1

Click on Significant Values for Screen Details ID:SGTC_8|Compound:0354-0001|FD-Score:4.64|P-value:9.84E-6 ID:SGTC_14|Compound:0354-0009|FD-Score:4.55|P-value:1.39E-5 ID:SGTC_43|Compound:0570-0105|FD-Score:3.77|P-value:2.59E-4 ID:SGTC_54|Compound:0159-0055|FD-Score:14.50|P-value:3.27E-41 ID:SGTC_205|Compound:1486-1330|FD-Score:3.43|P-value:8.10E-4 ID:SGTC_207|Compound:4185-0010|FD-Score:4.30|P-value:3.72E-5 ID:SGTC_503|Compound:tyrphostin 8|FD-Score:-3.43|P-value:7.46E-4 ID:SGTC_576|Compound:1073-0113|FD-Score:3.96|P-value:1.32E-4 ID:SGTC_734|Compound:2023-0417|FD-Score:4.01|P-value:1.11E-4 ID:SGTC_811|Compound:0416-0114|FD-Score:3.40|P-value:8.87E-4 ID:SGTC_935|Compound:2996-0153|FD-Score:5.40|P-value:3.24E-7 ID:SGTC_1064|Compound:honokiol|FD-Score:3.39|P-value:9.16E-4 ID:SGTC_1123|Compound:mangostin|FD-Score:-3.77|P-value:2.46E-4 ID:SGTC_1304|Compound:1165-0502|FD-Score:3.76|P-value:2.73E-4 ID:SGTC_1307|Compound:1187-1599|FD-Score:5.27|P-value:6.17E-7 ID:SGTC_1363|Compound:1636-0063|FD-Score:3.43|P-value:8.04E-4 ID:SGTC_1418|Compound:3966-3099|FD-Score:3.47|P-value:7.09E-4 ID:SGTC_1489|Compound:4065-0444|FD-Score:3.61|P-value:4.47E-4 ID:SGTC_1529|Compound:apigenin|FD-Score:-3.36|P-value:9.39E-4 ID:SGTC_1584|Compound:6-fluoro-dl-tryptophan|FD-Score:3.69|P-value:3.41E-4 ID:SGTC_1708|Compound:st033234|FD-Score:3.96|P-value:1.35E-4 ID:SGTC_1935|Compound:st074532|FD-Score:4.06|P-value:9.29E-5 ID:SGTC_2053|Compound:5265679|FD-Score:3.53|P-value:5.78E-4 ID:SGTC_2077|Compound:5212015|FD-Score:-3.50|P-value:5.93E-4 ID:SGTC_2290|Compound:7904846|FD-Score:3.39|P-value:9.12E-4 ID:SGTC_2444|Compound:5485484|FD-Score:6.89|P-value:1.06E-10 ID:SGTC_2501|Compound:sodium tetradecyl sulfate|FD-Score:6.50|P-value:1.05E-9 ID:SGTC_2972|Compound:9093619|FD-Score:-3.52|P-value:5.60E-4 ID:SGTC_2973|Compound:9094665|FD-Score:4.06|P-value:9.17E-5 ID:SGTC_3204|Compound:9114183|FD-Score:3.85|P-value:1.95E-4 ID:SGTC_3211|Compound:9128781|FD-Score:6.23|P-value:4.67E-9 ID:SGTC_3340|Compound:9146634|FD-Score:3.94|P-value:1.46E-4 ID:SGTC_8|Compound:0354-0001|FD-Score:4.64|P-value:9.84E-6 ID:SGTC_14|Compound:0354-0009|FD-Score:4.55|P-value:1.39E-5 ID:SGTC_43|Compound:0570-0105|FD-Score:3.77|P-value:2.59E-4 ID:SGTC_54|Compound:0159-0055|FD-Score:14.50|P-value:3.27E-41 ID:SGTC_205|Compound:1486-1330|FD-Score:3.43|P-value:8.10E-4 ID:SGTC_207|Compound:4185-0010|FD-Score:4.30|P-value:3.72E-5 ID:SGTC_503|Compound:tyrphostin 8|FD-Score:-3.43|P-value:7.46E-4 ID:SGTC_576|Compound:1073-0113|FD-Score:3.96|P-value:1.32E-4 ID:SGTC_734|Compound:2023-0417|FD-Score:4.01|P-value:1.11E-4 ID:SGTC_811|Compound:0416-0114|FD-Score:3.40|P-value:8.87E-4 ID:SGTC_935|Compound:2996-0153|FD-Score:5.40|P-value:3.24E-7 ID:SGTC_1064|Compound:honokiol|FD-Score:3.39|P-value:9.16E-4 ID:SGTC_1123|Compound:mangostin|FD-Score:-3.77|P-value:2.46E-4 ID:SGTC_1304|Compound:1165-0502|FD-Score:3.76|P-value:2.73E-4 ID:SGTC_1307|Compound:1187-1599|FD-Score:5.27|P-value:6.17E-7 ID:SGTC_1363|Compound:1636-0063|FD-Score:3.43|P-value:8.04E-4 ID:SGTC_1418|Compound:3966-3099|FD-Score:3.47|P-value:7.09E-4 ID:SGTC_1489|Compound:4065-0444|FD-Score:3.61|P-value:4.47E-4 ID:SGTC_1529|Compound:apigenin|FD-Score:-3.36|P-value:9.39E-4 ID:SGTC_1584|Compound:6-fluoro-dl-tryptophan|FD-Score:3.69|P-value:3.41E-4 ID:SGTC_1708|Compound:st033234|FD-Score:3.96|P-value:1.35E-4 ID:SGTC_1935|Compound:st074532|FD-Score:4.06|P-value:9.29E-5 ID:SGTC_2053|Compound:5265679|FD-Score:3.53|P-value:5.78E-4 ID:SGTC_2077|Compound:5212015|FD-Score:-3.50|P-value:5.93E-4 ID:SGTC_2290|Compound:7904846|FD-Score:3.39|P-value:9.12E-4 ID:SGTC_2444|Compound:5485484|FD-Score:6.89|P-value:1.06E-10 ID:SGTC_2501|Compound:sodium tetradecyl sulfate|FD-Score:6.50|P-value:1.05E-9 ID:SGTC_2972|Compound:9093619|FD-Score:-3.52|P-value:5.60E-4 ID:SGTC_2973|Compound:9094665|FD-Score:4.06|P-value:9.17E-5 ID:SGTC_3204|Compound:9114183|FD-Score:3.85|P-value:1.95E-4 ID:SGTC_3211|Compound:9128781|FD-Score:6.23|P-value:4.67E-9 ID:SGTC_3340|Compound:9146634|FD-Score:3.94|P-value:1.46E-4

Top fitness defect scores for YOL058W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_54 0159-0055 14.50 9.0 µM 3.27E-41 ERG2 4 4.30E-48
2 SGTC_2444 5485484 6.89 36.4 µM 1.06E-10 3 2.72E-12
3 SGTC_2501 sodium tetradecyl sulfate 6.50 13.4 µM 1.05E-9 6 4.01E-11
4 SGTC_3211 9128781 6.23 49.5 µM 4.67E-9 17 2.30E-10
5 SGTC_935 2996-0153 5.40 430.0 µM 3.24E-7 PDR1 6 3.28E-8
6 SGTC_1307 1187-1599 5.27 12.2 µM 6.17E-7 PDR1 7 6.98E-8
7 SGTC_8 0354-0001 4.64 70.0 µM 9.84E-6 54 1.78E-6
8 SGTC_14 0354-0009 4.55 295.0 µM 1.39E-5 17 2.67E-6
9 SGTC_207 4185-0010 4.30 4.5 µM 3.72E-5 endomembrane recycling 78 8.42E-6
10 SGTC_2973 9094665 4.06 18.0 µM 9.17E-5 NEO1 28 2.41E-5
11 SGTC_1935 st074532 4.06 41.6 µM 9.29E-5 27 2.45E-5
12 SGTC_734 2023-0417 4.01 80.0 µM 1.11E-4 ergosterol biosynthesis 14 3.02E-5
13 SGTC_576 1073-0113 3.96 22.7 µM 1.32E-4 redox potentiating 64 3.68E-5
14 SGTC_1708 st033234 3.96 31.8 µM 1.35E-4 17 3.78E-5
15 SGTC_3340 9146634 3.94 11.1 µM 1.46E-4 14 4.14E-5
16 SGTC_3204 9114183 3.85 49.5 µM 1.95E-4 38 5.83E-5
17 SGTC_43 0570-0105 3.77 72.5 µM 2.59E-4 ERG2 109 8.09E-5
18 SGTC_1304 1165-0502 3.76 251.0 µM 2.73E-4 65 8.60E-5
19 SGTC_1584 6-fluoro-dl-tryptophan 3.69 90.0 µM 3.41E-4 mitochondrial processes 20 1.12E-4
20 SGTC_1489 4065-0444 3.61 182.0 µM 4.47E-4 28 1.53E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.303 3.76E-72 YJL088W ARG3 hom Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine
0.296 1.76E-68 YOL140W ARG8 hom Acetylornithine aminotransferase, catalyzes the fourth step in the biosynthesis of the arginine precursor ornithine
0.198 4.51E-31 YHR018C ARG4 hom Argininosuccinate lyase, catalyzes the final step in the arginine biosynthesis pathway
0.182 2.68E-26 YER069W ARG5,6 hom Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine
0.158 3.54E-20 YOR130C ORT1 hom Ornithine transporter of the mitochondrial inner membrane, exports ornithine from mitochondria as part of arginine biosynthesis; human ortholog is associated with hyperammonaemia-hyperornithinaemia-homocitrullinuria (HHH) syndrome
0.145 2.70E-17 YDR340W_d YDR340W_d hom Putative protein of unknown function
0.145 3.67E-17 YER068C-A_d YER068C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.142 1.49E-16 YJL071W ARG2 hom Acetylglutamate synthase (glutamate N-acetyltransferase), mitochondrial enzyme that catalyzes the first step in the biosynthesis of the arginine precursor ornithine; forms a complex with Arg5,6p
0.141 2.00E-16 YOR303W CPA1 hom Small subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor; translationally regulated by an attenuator peptide encoded by YOR302W within the CPA1 mRNA 5'-leader
0.134 5.40E-15 YJR109C CPA2 hom Large subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor
0.108 3.79E-10 YOR323C PRO2 hom Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis
0.104 1.70E-9 YFR023W PES4 hom Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication
0.101 4.50E-9 YEL009C GCN4 hom Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
0.097 1.74E-8 YML062C MFT1 hom Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance
0.097 1.81E-8 YCL057C-A MOS1 hom Mitochondrial protein essential for proper inner membrane organization; conserved component of the mitochondrial inner membrane organizing system (MICOS, MINOS, or MitOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture; ortholog of human MINOS1