YOL071W / EMI5

Subunit of succinate dehydrogenase, which couples succinate oxidation to ubiquinone reduction; required for FAD cofactor attachment to Sdh1p; mutations in human ortholog PGL2 are associated with neuroendocrine tumors (paraganglioma)

Zygosity: Homozygous strain
fixedexpanded
Profile for YOL071W / EMI5

Click on Significant Values for Screen Details ID:SGTC_36|Compound:0335-0881|FD-Score:4.67|P-value:1.10E-7 ID:SGTC_67|Compound:0335-0847|FD-Score:3.76|P-value:1.42E-5 ID:SGTC_134|Compound:0269-0018|FD-Score:2.91|P-value:5.63E-4 ID:SGTC_139|Compound:0099-0334|FD-Score:-2.99|P-value:6.58E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:3.62|P-value:2.73E-5 ID:SGTC_257|Compound:daunorubicin|FD-Score:-3.09|P-value:4.48E-4 ID:SGTC_271|Compound:rotenone|FD-Score:4.43|P-value:4.27E-7 ID:SGTC_275|Compound:trichlorophene|FD-Score:3.01|P-value:3.79E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:7.05|P-value:3.18E-15 ID:SGTC_383|Compound:2324-0395|FD-Score:4.07|P-value:2.96E-6 ID:SGTC_399|Compound:oxolinic acid|FD-Score:-3.03|P-value:5.67E-4 ID:SGTC_516|Compound:3992-2719|FD-Score:-4.49|P-value:6.10E-7 ID:SGTC_610|Compound:4215-0184|FD-Score:-5.27|P-value:5.49E-9 ID:SGTC_636|Compound:0kpi-0074|FD-Score:3.62|P-value:2.72E-5 ID:SGTC_831|Compound:k061-1017|FD-Score:3.76|P-value:1.39E-5 ID:SGTC_996|Compound:3970-0799|FD-Score:2.82|P-value:7.94E-4 ID:SGTC_1245|Compound:0418-0076|FD-Score:4.50|P-value:2.92E-7 ID:SGTC_1246|Compound:0422-0085|FD-Score:4.28|P-value:9.79E-7 ID:SGTC_1249|Compound:0481-0015|FD-Score:-2.98|P-value:6.75E-4 ID:SGTC_1337|Compound:1486-1011|FD-Score:-3.30|P-value:1.88E-4 ID:SGTC_1367|Compound:1812-0510|FD-Score:-2.92|P-value:8.44E-4 ID:SGTC_1453|Compound:4237-0541|FD-Score:2.78|P-value:9.38E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.45|P-value:1.03E-4 ID:SGTC_1463|Compound:k081-0032|FD-Score:-2.99|P-value:6.50E-4 ID:SGTC_1726|Compound:st035556|FD-Score:4.36|P-value:6.27E-7 ID:SGTC_1727|Compound:st034309|FD-Score:3.11|P-value:2.60E-4 ID:SGTC_1731|Compound:st035964|FD-Score:-3.76|P-value:2.44E-5 ID:SGTC_1737|Compound:st035557|FD-Score:-3.59|P-value:5.32E-5 ID:SGTC_1898|Compound:st060199|FD-Score:-3.25|P-value:2.32E-4 ID:SGTC_1904|Compound:st060225|FD-Score:-3.30|P-value:1.89E-4 ID:SGTC_1906|Compound:st060206|FD-Score:2.94|P-value:5.08E-4 ID:SGTC_1948|Compound:st076329|FD-Score:4.02|P-value:3.88E-6 ID:SGTC_2309|Compound:7664088|FD-Score:3.17|P-value:2.03E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:-4.60|P-value:3.29E-7 ID:SGTC_2491|Compound:paeonol|FD-Score:2.97|P-value:4.40E-4 ID:SGTC_2493|Compound:resveratrol|FD-Score:4.22|P-value:1.38E-6 ID:SGTC_2691|Compound:st077356|FD-Score:3.25|P-value:1.41E-4 ID:SGTC_2800|Compound:7887935|FD-Score:3.58|P-value:3.24E-5 ID:SGTC_3212|Compound:9128307|FD-Score:-3.96|P-value:9.55E-6 ID:SGTC_36|Compound:0335-0881|FD-Score:4.67|P-value:1.10E-7 ID:SGTC_67|Compound:0335-0847|FD-Score:3.76|P-value:1.42E-5 ID:SGTC_134|Compound:0269-0018|FD-Score:2.91|P-value:5.63E-4 ID:SGTC_139|Compound:0099-0334|FD-Score:-2.99|P-value:6.58E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:3.62|P-value:2.73E-5 ID:SGTC_257|Compound:daunorubicin|FD-Score:-3.09|P-value:4.48E-4 ID:SGTC_271|Compound:rotenone|FD-Score:4.43|P-value:4.27E-7 ID:SGTC_275|Compound:trichlorophene|FD-Score:3.01|P-value:3.79E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:7.05|P-value:3.18E-15 ID:SGTC_383|Compound:2324-0395|FD-Score:4.07|P-value:2.96E-6 ID:SGTC_399|Compound:oxolinic acid|FD-Score:-3.03|P-value:5.67E-4 ID:SGTC_516|Compound:3992-2719|FD-Score:-4.49|P-value:6.10E-7 ID:SGTC_610|Compound:4215-0184|FD-Score:-5.27|P-value:5.49E-9 ID:SGTC_636|Compound:0kpi-0074|FD-Score:3.62|P-value:2.72E-5 ID:SGTC_831|Compound:k061-1017|FD-Score:3.76|P-value:1.39E-5 ID:SGTC_996|Compound:3970-0799|FD-Score:2.82|P-value:7.94E-4 ID:SGTC_1245|Compound:0418-0076|FD-Score:4.50|P-value:2.92E-7 ID:SGTC_1246|Compound:0422-0085|FD-Score:4.28|P-value:9.79E-7 ID:SGTC_1249|Compound:0481-0015|FD-Score:-2.98|P-value:6.75E-4 ID:SGTC_1337|Compound:1486-1011|FD-Score:-3.30|P-value:1.88E-4 ID:SGTC_1367|Compound:1812-0510|FD-Score:-2.92|P-value:8.44E-4 ID:SGTC_1453|Compound:4237-0541|FD-Score:2.78|P-value:9.38E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.45|P-value:1.03E-4 ID:SGTC_1463|Compound:k081-0032|FD-Score:-2.99|P-value:6.50E-4 ID:SGTC_1726|Compound:st035556|FD-Score:4.36|P-value:6.27E-7 ID:SGTC_1727|Compound:st034309|FD-Score:3.11|P-value:2.60E-4 ID:SGTC_1731|Compound:st035964|FD-Score:-3.76|P-value:2.44E-5 ID:SGTC_1737|Compound:st035557|FD-Score:-3.59|P-value:5.32E-5 ID:SGTC_1898|Compound:st060199|FD-Score:-3.25|P-value:2.32E-4 ID:SGTC_1904|Compound:st060225|FD-Score:-3.30|P-value:1.89E-4 ID:SGTC_1906|Compound:st060206|FD-Score:2.94|P-value:5.08E-4 ID:SGTC_1948|Compound:st076329|FD-Score:4.02|P-value:3.88E-6 ID:SGTC_2309|Compound:7664088|FD-Score:3.17|P-value:2.03E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:-4.60|P-value:3.29E-7 ID:SGTC_2491|Compound:paeonol|FD-Score:2.97|P-value:4.40E-4 ID:SGTC_2493|Compound:resveratrol|FD-Score:4.22|P-value:1.38E-6 ID:SGTC_2691|Compound:st077356|FD-Score:3.25|P-value:1.41E-4 ID:SGTC_2800|Compound:7887935|FD-Score:3.58|P-value:3.24E-5 ID:SGTC_3212|Compound:9128307|FD-Score:-3.96|P-value:9.55E-6

Top fitness defect scores for YOL071W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
1 SGTC_358 0075-0058 7.05 9.4 µM 3.18E-15 8 8.81E-13
2 SGTC_36 0335-0881 4.67 14.2 µM 1.10E-7 ubiquinone biosynthesis & proteasome 88 1.53E-6
3 SGTC_1245 0418-0076 4.50 17.2 µM 2.92E-7 ERG2 20 3.43E-6
4 SGTC_271 rotenone 4.43 740.0 µM 4.27E-7 15 4.70E-6
5 SGTC_1726 st035556 4.36 14.6 µM 6.27E-7 26 6.46E-6
6 SGTC_1246 0422-0085 4.28 105.0 µM 9.79E-7 20 9.34E-6
7 SGTC_2493 resveratrol 4.22 26.1 µM 1.38E-6 4 1.24E-5
8 SGTC_383 2324-0395 4.07 7.4 µM 2.96E-6 azole & statin 32 2.33E-5
9 SGTC_1948 st076329 4.02 66.6 µM 3.88E-6 28 2.92E-5
10 SGTC_831 k061-1017 3.76 217.0 µM 1.39E-5 translation 75 8.41E-5
11 SGTC_67 0335-0847 3.76 266.0 µM 1.42E-5 ubiquinone biosynthesis & proteasome 130 8.56E-5
12 SGTC_636 0kpi-0074 3.62 126.0 µM 2.72E-5 27 1.46E-4
13 SGTC_163 1327-0036 3.62 7.5 µM 2.73E-5 131 1.47E-4
14 SGTC_2800 7887935 3.58 81.8 µM 3.24E-5 RPP1 & pyrimidine depletion 46 1.69E-4
15 SGTC_2691 st077356 3.25 60.2 µM 1.41E-4 64 5.72E-4
16 SGTC_2309 7664088 3.17 163.9 µM 2.03E-4 89 7.73E-4
17 SGTC_1727 st034309 3.11 9.9 µM 2.60E-4 94 9.48E-4
18 SGTC_275 trichlorophene 3.01 5.7 µM 3.79E-4 mitochondrial stress 298 0.00130
19 SGTC_2491 paeonol 2.97 100.0 µM 4.40E-4 25 0.00147
20 SGTC_1906 st060206 2.94 94.7 µM 5.08E-4 70 0.00165

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.204 8.00E-33 YBR169C SSE2 hom Member of the heat shock protein 70 (HSP70) family; may be involved in protein folding; localized to the cytoplasm; SSE2 has a paralog, SSE1, that arose from the whole genome duplication
0.175 1.29E-24 YGR123C PPT1 hom Protein serine/threonine phosphatase, regulates Hsp90 chaperone by affecting its ATPase and cochaperone binding activities; has similarity to human phosphatase PP5; present in both the nucleus and cytoplasm; expressed during logarithmic growth
0.167 2.19E-22 YPR024W YME1 hom Catalytic subunit of the mitochondrial inner membrane i-AAA protease complex, which is responsible for degradation of unfolded or misfolded mitochondrial gene products; also has a role in intermembrane space protein folding; mutation causes an elevated rate of mitochondrial turnover
0.163 1.64E-21 YMR322C_p SNO4_p hom Possible chaperone and cysteine protease, similar to bacterial Hsp31 and yeast Hsp31p, Hsp32p, and Hsp33p; DJ-1/ThiJ/PfpI superfamily member; predicted involvement in pyridoxine metabolism; induced by mild heat stress and copper deprivation
0.162 3.27E-21 YOR302W YOR302W hom CPA1 uORF, Arginine attenuator peptide, regulates translation of the CPA1 mRNA
0.153 4.76E-19 YPR151C SUE1 hom Mitochondrial protein required for degradation of unstable forms of cytochrome c
0.152 7.97E-19 YBR165W UBS1 hom Ubiquitin-conjugating enzyme suppressor that functions as a general positive regulator of Cdc34p activity; nuclear protein that may represent a link between nucleocytoplasmic transport and ubiquitin ligase activity
0.150 2.38E-18 YHR131C_p YHR131C_p hom Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; overexpression causes cell cycle delay or arrest; contains a PH domain and binds phosphatidylinositols and other lipids in a large-scale study
0.149 3.86E-18 YLR237W THI7 hom Plasma membrane transporter responsible for the uptake of thiamine, member of the major facilitator superfamily of transporters; mutation of human ortholog causes thiamine-responsive megaloblastic anemia
0.147 1.12E-17 YOL080C REX4 hom Putative RNA exonuclease possibly involved in pre-rRNA processing and ribosome assembly
0.141 1.91E-16 YFL004W VTC2 hom Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion
0.138 8.69E-16 YNR042W_d YNR042W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2
0.137 1.96E-15 YGR176W_d YGR176W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.136 2.81E-15 YMR110C HFD1 hom Hexadecenal dehydrogenase; involved in the conversion of sphingosine 1-phosphate breakdown product hexadecenal to hexadecenoic acid; located in the mitochondrial outer membrane and also in lipid particles; has similarity to ALDH3A2, a human fatty aldehyde dehydrogenase (FALDH) mutated in Sjogren-Larsson syndrome, a neurocutaneous disorder
0.131 2.13E-14 YJL072C PSF2 het Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery