YOL100W / PKH2

Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p

Zygosity: Homozygous strain
fixedexpanded
Profile for YOL100W / PKH2

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:-4.66|P-value:5.33E-6 ID:SGTC_66|Compound:0109-0187|FD-Score:3.53|P-value:3.17E-4 ID:SGTC_73|Compound:0327-0325|FD-Score:3.40|P-value:5.11E-4 ID:SGTC_75|Compound:0159-0059|FD-Score:-3.31|P-value:9.06E-4 ID:SGTC_121|Compound:0557-0495|FD-Score:-3.47|P-value:5.42E-4 ID:SGTC_253|Compound:dyclonine|FD-Score:3.90|P-value:8.23E-5 ID:SGTC_263|Compound:alizarin|FD-Score:4.08|P-value:4.07E-5 ID:SGTC_265|Compound:mycophenolic acid|FD-Score:3.53|P-value:3.19E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:3.23|P-value:8.94E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:-4.60|P-value:6.76E-6 ID:SGTC_442|Compound:cape|FD-Score:-4.07|P-value:5.95E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.28|P-value:7.58E-4 ID:SGTC_525|Compound:k781-2675|FD-Score:3.72|P-value:1.63E-4 ID:SGTC_527|Compound:4476-4215|FD-Score:3.28|P-value:7.45E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:4.00|P-value:5.68E-5 ID:SGTC_671|Compound:0560-0003|FD-Score:3.45|P-value:4.27E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:5.11|P-value:4.32E-7 ID:SGTC_812|Compound:4251-0442|FD-Score:3.29|P-value:7.31E-4 ID:SGTC_942|Compound:3253-1115|FD-Score:3.78|P-value:1.31E-4 ID:SGTC_944|Compound:3370-0224|FD-Score:-3.41|P-value:6.62E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:-3.38|P-value:7.36E-4 ID:SGTC_1117|Compound:retinoic acid|FD-Score:3.42|P-value:4.62E-4 ID:SGTC_1221|Compound:2958-7315|FD-Score:-3.39|P-value:6.91E-4 ID:SGTC_1225|Compound:1216-0074|FD-Score:-3.51|P-value:4.62E-4 ID:SGTC_1231|Compound:0242-0792|FD-Score:5.40|P-value:1.02E-7 ID:SGTC_1335|Compound:1486-0900|FD-Score:3.86|P-value:9.46E-5 ID:SGTC_1564|Compound:D-Arginine|FD-Score:-3.39|P-value:7.02E-4 ID:SGTC_1576|Compound:erythromycin ethylsuccinate|FD-Score:3.55|P-value:2.98E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:-3.56|P-value:3.95E-4 ID:SGTC_1650|Compound:st011916|FD-Score:4.09|P-value:3.92E-5 ID:SGTC_1712|Compound:st032288|FD-Score:-3.60|P-value:3.45E-4 ID:SGTC_1728|Compound:st035614|FD-Score:4.44|P-value:9.07E-6 ID:SGTC_1745|Compound:st081495|FD-Score:-5.40|P-value:1.60E-7 ID:SGTC_1754|Compound:st042065|FD-Score:-4.10|P-value:5.32E-5 ID:SGTC_1834|Compound:st055981|FD-Score:-3.68|P-value:2.58E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:5.01|P-value:7.05E-7 ID:SGTC_1876|Compound:myriocin|FD-Score:3.98|P-value:6.14E-5 ID:SGTC_2148|Compound:5546263|FD-Score:3.35|P-value:5.96E-4 ID:SGTC_2177|Compound:5811945|FD-Score:3.44|P-value:4.31E-4 ID:SGTC_2248|Compound:tofa|FD-Score:3.62|P-value:2.30E-4 ID:SGTC_2275|Compound:7947463|FD-Score:3.20|P-value:9.72E-4 ID:SGTC_2286|Compound:7945697|FD-Score:-3.58|P-value:3.67E-4 ID:SGTC_2347|Compound:9049783|FD-Score:3.85|P-value:1.01E-4 ID:SGTC_2380|Compound:9035439|FD-Score:3.38|P-value:5.30E-4 ID:SGTC_2389|Compound:9091542|FD-Score:3.86|P-value:9.49E-5 ID:SGTC_2477|Compound:5967686|FD-Score:4.01|P-value:5.37E-5 ID:SGTC_2556|Compound:citropten|FD-Score:3.73|P-value:1.57E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:4.51|P-value:6.82E-6 ID:SGTC_2702|Compound:st077768|FD-Score:5.26|P-value:2.02E-7 ID:SGTC_2756|Compound:carbadox|FD-Score:-6.40|P-value:6.32E-10 ID:SGTC_2758|Compound:dehydroepiandrosterone|FD-Score:-3.62|P-value:3.17E-4 ID:SGTC_2764|Compound:methyldopa|FD-Score:5.96|P-value:4.93E-9 ID:SGTC_2767|Compound:sibutramine|FD-Score:-3.76|P-value:1.92E-4 ID:SGTC_2814|Compound:7977482|FD-Score:3.32|P-value:6.62E-4 ID:SGTC_2869|Compound:9037858|FD-Score:3.45|P-value:4.27E-4 ID:SGTC_2883|Compound:9059145|FD-Score:4.03|P-value:5.05E-5 ID:SGTC_2942|Compound:9052777|FD-Score:3.33|P-value:6.44E-4 ID:SGTC_3012|Compound:9080433|FD-Score:5.04|P-value:5.91E-7 ID:SGTC_3026|Compound:9090633|FD-Score:6.34|P-value:5.44E-10 ID:SGTC_3037|Compound:9091026|FD-Score:-5.39|P-value:1.65E-7 ID:SGTC_3039|Compound:9090270|FD-Score:4.06|P-value:4.48E-5 ID:SGTC_3091|Compound:9116372|FD-Score:3.83|P-value:1.09E-4 ID:SGTC_3117|Compound:9124945|FD-Score:3.32|P-value:6.67E-4 ID:SGTC_3145|Compound:9085516|FD-Score:-3.95|P-value:9.73E-5 ID:SGTC_3248|Compound:9135562|FD-Score:3.38|P-value:5.44E-4 ID:SGTC_3266|Compound:9136860|FD-Score:-4.01|P-value:7.71E-5 ID:SGTC_3288|Compound:9119690|FD-Score:-3.46|P-value:5.53E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-4.66|P-value:5.33E-6 ID:SGTC_66|Compound:0109-0187|FD-Score:3.53|P-value:3.17E-4 ID:SGTC_73|Compound:0327-0325|FD-Score:3.40|P-value:5.11E-4 ID:SGTC_75|Compound:0159-0059|FD-Score:-3.31|P-value:9.06E-4 ID:SGTC_121|Compound:0557-0495|FD-Score:-3.47|P-value:5.42E-4 ID:SGTC_253|Compound:dyclonine|FD-Score:3.90|P-value:8.23E-5 ID:SGTC_263|Compound:alizarin|FD-Score:4.08|P-value:4.07E-5 ID:SGTC_265|Compound:mycophenolic acid|FD-Score:3.53|P-value:3.19E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:3.23|P-value:8.94E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:-4.60|P-value:6.76E-6 ID:SGTC_442|Compound:cape|FD-Score:-4.07|P-value:5.95E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.28|P-value:7.58E-4 ID:SGTC_525|Compound:k781-2675|FD-Score:3.72|P-value:1.63E-4 ID:SGTC_527|Compound:4476-4215|FD-Score:3.28|P-value:7.45E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:4.00|P-value:5.68E-5 ID:SGTC_671|Compound:0560-0003|FD-Score:3.45|P-value:4.27E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:5.11|P-value:4.32E-7 ID:SGTC_812|Compound:4251-0442|FD-Score:3.29|P-value:7.31E-4 ID:SGTC_942|Compound:3253-1115|FD-Score:3.78|P-value:1.31E-4 ID:SGTC_944|Compound:3370-0224|FD-Score:-3.41|P-value:6.62E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:-3.38|P-value:7.36E-4 ID:SGTC_1117|Compound:retinoic acid|FD-Score:3.42|P-value:4.62E-4 ID:SGTC_1221|Compound:2958-7315|FD-Score:-3.39|P-value:6.91E-4 ID:SGTC_1225|Compound:1216-0074|FD-Score:-3.51|P-value:4.62E-4 ID:SGTC_1231|Compound:0242-0792|FD-Score:5.40|P-value:1.02E-7 ID:SGTC_1335|Compound:1486-0900|FD-Score:3.86|P-value:9.46E-5 ID:SGTC_1564|Compound:D-Arginine|FD-Score:-3.39|P-value:7.02E-4 ID:SGTC_1576|Compound:erythromycin ethylsuccinate|FD-Score:3.55|P-value:2.98E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:-3.56|P-value:3.95E-4 ID:SGTC_1650|Compound:st011916|FD-Score:4.09|P-value:3.92E-5 ID:SGTC_1712|Compound:st032288|FD-Score:-3.60|P-value:3.45E-4 ID:SGTC_1728|Compound:st035614|FD-Score:4.44|P-value:9.07E-6 ID:SGTC_1745|Compound:st081495|FD-Score:-5.40|P-value:1.60E-7 ID:SGTC_1754|Compound:st042065|FD-Score:-4.10|P-value:5.32E-5 ID:SGTC_1834|Compound:st055981|FD-Score:-3.68|P-value:2.58E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:5.01|P-value:7.05E-7 ID:SGTC_1876|Compound:myriocin|FD-Score:3.98|P-value:6.14E-5 ID:SGTC_2148|Compound:5546263|FD-Score:3.35|P-value:5.96E-4 ID:SGTC_2177|Compound:5811945|FD-Score:3.44|P-value:4.31E-4 ID:SGTC_2248|Compound:tofa|FD-Score:3.62|P-value:2.30E-4 ID:SGTC_2275|Compound:7947463|FD-Score:3.20|P-value:9.72E-4 ID:SGTC_2286|Compound:7945697|FD-Score:-3.58|P-value:3.67E-4 ID:SGTC_2347|Compound:9049783|FD-Score:3.85|P-value:1.01E-4 ID:SGTC_2380|Compound:9035439|FD-Score:3.38|P-value:5.30E-4 ID:SGTC_2389|Compound:9091542|FD-Score:3.86|P-value:9.49E-5 ID:SGTC_2477|Compound:5967686|FD-Score:4.01|P-value:5.37E-5 ID:SGTC_2556|Compound:citropten|FD-Score:3.73|P-value:1.57E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:4.51|P-value:6.82E-6 ID:SGTC_2702|Compound:st077768|FD-Score:5.26|P-value:2.02E-7 ID:SGTC_2756|Compound:carbadox|FD-Score:-6.40|P-value:6.32E-10 ID:SGTC_2758|Compound:dehydroepiandrosterone|FD-Score:-3.62|P-value:3.17E-4 ID:SGTC_2764|Compound:methyldopa|FD-Score:5.96|P-value:4.93E-9 ID:SGTC_2767|Compound:sibutramine|FD-Score:-3.76|P-value:1.92E-4 ID:SGTC_2814|Compound:7977482|FD-Score:3.32|P-value:6.62E-4 ID:SGTC_2869|Compound:9037858|FD-Score:3.45|P-value:4.27E-4 ID:SGTC_2883|Compound:9059145|FD-Score:4.03|P-value:5.05E-5 ID:SGTC_2942|Compound:9052777|FD-Score:3.33|P-value:6.44E-4 ID:SGTC_3012|Compound:9080433|FD-Score:5.04|P-value:5.91E-7 ID:SGTC_3026|Compound:9090633|FD-Score:6.34|P-value:5.44E-10 ID:SGTC_3037|Compound:9091026|FD-Score:-5.39|P-value:1.65E-7 ID:SGTC_3039|Compound:9090270|FD-Score:4.06|P-value:4.48E-5 ID:SGTC_3091|Compound:9116372|FD-Score:3.83|P-value:1.09E-4 ID:SGTC_3117|Compound:9124945|FD-Score:3.32|P-value:6.67E-4 ID:SGTC_3145|Compound:9085516|FD-Score:-3.95|P-value:9.73E-5 ID:SGTC_3248|Compound:9135562|FD-Score:3.38|P-value:5.44E-4 ID:SGTC_3266|Compound:9136860|FD-Score:-4.01|P-value:7.71E-5 ID:SGTC_3288|Compound:9119690|FD-Score:-3.46|P-value:5.53E-4

Top fitness defect scores for YOL100W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3026 9090633 6.34 49.5 µM 5.44E-10 3 1.15E-10
2 SGTC_2764 methyldopa 5.96 94.7 µM 4.93E-9 6 1.26E-9
3 SGTC_1231 0242-0792 5.40 125.0 µM 1.02E-7 4 3.41E-8
4 SGTC_2702 st077768 5.26 96.8 µM 2.02E-7 6 7.13E-8
5 SGTC_800 3448-7362 5.11 155.0 µM 4.32E-7 translation 84 1.62E-7
6 SGTC_3012 9080433 5.04 71.4 µM 5.91E-7 2 2.28E-7
7 SGTC_1844 rapamycin 5.01 4.0 nM 7.05E-7 115 2.77E-7
8 SGTC_2679 rapamycin 4.51 1.0 nM 6.82E-6 117 3.23E-6
9 SGTC_1728 st035614 4.44 33.8 µM 9.07E-6 30 4.40E-6
10 SGTC_1650 st011916 4.09 45.8 µM 3.92E-5 9 2.15E-5
11 SGTC_263 alizarin 4.08 107.9 µM 4.07E-5 iron homeostasis 57 2.23E-5
12 SGTC_3039 9090270 4.06 49.5 µM 4.48E-5 RPP1 & pyrimidine depletion 8 2.48E-5
13 SGTC_2883 9059145 4.03 45.5 µM 5.05E-5 8 2.82E-5
14 SGTC_2477 5967686 4.01 25.2 µM 5.37E-5 25 3.01E-5
15 SGTC_625 k213-0058 4.00 121.0 µM 5.68E-5 translation 102 3.20E-5
16 SGTC_1876 myriocin 3.98 605.0 nM 6.14E-5 30 3.49E-5
17 SGTC_253 dyclonine 3.90 31.2 µM 8.23E-5 amide catabolism 28 4.78E-5
18 SGTC_1335 1486-0900 3.86 202.0 µM 9.46E-5 27 5.56E-5
19 SGTC_2389 9091542 3.86 195.5 µM 9.49E-5 iron homeostasis 34 5.57E-5
20 SGTC_2347 9049783 3.85 174.8 µM 1.01E-4 34 5.95E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.253 2.49E-50 YPL208W RKM1 hom SET-domain lysine-N-methyltransferase, catalyzes the formation of dimethyllysine residues on the large ribsomal subunit proteins L23a (Rpl23Ap and Rpl23Bp) and L18 (Rps18Ap and Rps18Bp)
0.192 2.58E-29 YIL039W TED1 hom Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
0.166 3.09E-22 YGL236C MTO1 hom Mitochondrial protein, forms a heterodimer complex with Mss1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; required for respiration in paromomycin-resistant 15S rRNA mutants
0.161 6.35E-21 YKL110C KTI12 hom Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p
0.157 4.92E-20 YGL251C HFM1 hom Meiosis specific DNA helicase involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity
0.144 6.16E-17 YBR162C TOS1 hom Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C
0.143 8.72E-17 YKL203C TOR2 het PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
0.143 9.53E-17 YDR516C EMI2 hom Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.134 6.21E-15 YLR384C IKI3 hom Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD)
0.110 1.55E-10 YGL211W NCS6 hom Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
0.110 1.95E-10 YJR066W TOR1 hom PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication
0.108 4.09E-10 YLR287C-A RPS30A hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication
0.105 1.07E-9 YPL101W ELP4 hom Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity
0.104 1.29E-9 YOL127W RPL25 het Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23
0.103 2.09E-9 YMR296C LCB1 het Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine