YOL125W / TRM13

2'-O-methyltransferase responsible for modification of tRNA at position 4; C-terminal domain has similarity to Rossmann-fold (RFM) superfamily of RNA methyltransferases

Zygosity: Homozygous strain
fixedexpanded
Profile for YOL125W / TRM13

Click on Significant Values for Screen Details ID:SGTC_166|Compound:k072-0058|FD-Score:-2.72|P-value:7.15E-4 ID:SGTC_196|Compound:k015-0023|FD-Score:-2.97|P-value:2.52E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:-2.96|P-value:2.58E-4 ID:SGTC_420|Compound:mitomycin C|FD-Score:-3.39|P-value:3.51E-5 ID:SGTC_859|Compound:0439-0102|FD-Score:2.86|P-value:3.51E-4 ID:SGTC_865|Compound:0488-0499|FD-Score:3.56|P-value:1.29E-5 ID:SGTC_873|Compound:0591-4978|FD-Score:2.65|P-value:8.53E-4 ID:SGTC_875|Compound:0709-0210|FD-Score:2.98|P-value:2.10E-4 ID:SGTC_1004|Compound:4084-0029|FD-Score:2.73|P-value:6.30E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:-3.18|P-value:9.85E-5 ID:SGTC_1095|Compound:3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran|FD-Score:-2.95|P-value:2.76E-4 ID:SGTC_1124|Compound:fisetin|FD-Score:3.54|P-value:1.43E-5 ID:SGTC_1222|Compound:0366-0074|FD-Score:3.30|P-value:4.78E-5 ID:SGTC_1232|Compound:0279-0259|FD-Score:-2.90|P-value:3.33E-4 ID:SGTC_1282|Compound:0886-0010|FD-Score:4.23|P-value:2.92E-7 ID:SGTC_1285|Compound:0929-0069|FD-Score:-3.11|P-value:1.37E-4 ID:SGTC_1370|Compound:2001-0092|FD-Score:2.81|P-value:4.49E-4 ID:SGTC_1395|Compound:3486-0190|FD-Score:3.12|P-value:1.11E-4 ID:SGTC_1405|Compound:3909-7894|FD-Score:2.62|P-value:9.82E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.23|P-value:7.57E-5 ID:SGTC_1700|Compound:st030840|FD-Score:2.77|P-value:5.23E-4 ID:SGTC_1701|Compound:st028638|FD-Score:2.90|P-value:3.02E-4 ID:SGTC_1733|Compound:st037357|FD-Score:-2.78|P-value:5.68E-4 ID:SGTC_1994|Compound:st072947|FD-Score:3.30|P-value:4.71E-5 ID:SGTC_1997|Compound:st073024|FD-Score:2.81|P-value:4.37E-4 ID:SGTC_2027|Compound:5150920|FD-Score:3.63|P-value:8.77E-6 ID:SGTC_2029|Compound:5175081|FD-Score:-3.20|P-value:8.86E-5 ID:SGTC_2031|Compound:5180455|FD-Score:-3.12|P-value:1.26E-4 ID:SGTC_2046|Compound:4110841|FD-Score:3.59|P-value:1.10E-5 ID:SGTC_2264|Compound:7948757|FD-Score:-2.69|P-value:8.28E-4 ID:SGTC_2274|Compound:7947094|FD-Score:-2.82|P-value:4.70E-4 ID:SGTC_2304|Compound:7443016|FD-Score:-4.10|P-value:7.43E-7 ID:SGTC_2309|Compound:7664088|FD-Score:3.34|P-value:3.96E-5 ID:SGTC_2319|Compound:6033012|FD-Score:-2.67|P-value:8.94E-4 ID:SGTC_2323|Compound:6148992|FD-Score:-3.23|P-value:7.71E-5 ID:SGTC_2372|Compound:9071633|FD-Score:2.86|P-value:3.63E-4 ID:SGTC_2400|Compound:5203494|FD-Score:-2.97|P-value:2.48E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:2.71|P-value:6.84E-4 ID:SGTC_2564|Compound:betulinic acid|FD-Score:3.73|P-value:5.24E-6 ID:SGTC_2568|Compound:diosmetin|FD-Score:3.88|P-value:2.25E-6 ID:SGTC_2570|Compound:licareol|FD-Score:-4.86|P-value:5.67E-9 ID:SGTC_2575|Compound:plumbagin|FD-Score:2.71|P-value:6.73E-4 ID:SGTC_2579|Compound:2',5'-dihydroxy-4-methoxychalcone|FD-Score:-5.00|P-value:2.23E-9 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:3.64|P-value:8.30E-6 ID:SGTC_2787|Compound:5284540|FD-Score:3.78|P-value:3.98E-6 ID:SGTC_2811|Compound:7967822|FD-Score:2.95|P-value:2.44E-4 ID:SGTC_2815|Compound:7968870|FD-Score:-3.24|P-value:7.45E-5 ID:SGTC_2936|Compound:9038235|FD-Score:-3.23|P-value:7.78E-5 ID:SGTC_2989|Compound:9073649|FD-Score:3.84|P-value:2.81E-6 ID:SGTC_166|Compound:k072-0058|FD-Score:-2.72|P-value:7.15E-4 ID:SGTC_196|Compound:k015-0023|FD-Score:-2.97|P-value:2.52E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:-2.96|P-value:2.58E-4 ID:SGTC_420|Compound:mitomycin C|FD-Score:-3.39|P-value:3.51E-5 ID:SGTC_859|Compound:0439-0102|FD-Score:2.86|P-value:3.51E-4 ID:SGTC_865|Compound:0488-0499|FD-Score:3.56|P-value:1.29E-5 ID:SGTC_873|Compound:0591-4978|FD-Score:2.65|P-value:8.53E-4 ID:SGTC_875|Compound:0709-0210|FD-Score:2.98|P-value:2.10E-4 ID:SGTC_1004|Compound:4084-0029|FD-Score:2.73|P-value:6.30E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:-3.18|P-value:9.85E-5 ID:SGTC_1095|Compound:3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran|FD-Score:-2.95|P-value:2.76E-4 ID:SGTC_1124|Compound:fisetin|FD-Score:3.54|P-value:1.43E-5 ID:SGTC_1222|Compound:0366-0074|FD-Score:3.30|P-value:4.78E-5 ID:SGTC_1232|Compound:0279-0259|FD-Score:-2.90|P-value:3.33E-4 ID:SGTC_1282|Compound:0886-0010|FD-Score:4.23|P-value:2.92E-7 ID:SGTC_1285|Compound:0929-0069|FD-Score:-3.11|P-value:1.37E-4 ID:SGTC_1370|Compound:2001-0092|FD-Score:2.81|P-value:4.49E-4 ID:SGTC_1395|Compound:3486-0190|FD-Score:3.12|P-value:1.11E-4 ID:SGTC_1405|Compound:3909-7894|FD-Score:2.62|P-value:9.82E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.23|P-value:7.57E-5 ID:SGTC_1700|Compound:st030840|FD-Score:2.77|P-value:5.23E-4 ID:SGTC_1701|Compound:st028638|FD-Score:2.90|P-value:3.02E-4 ID:SGTC_1733|Compound:st037357|FD-Score:-2.78|P-value:5.68E-4 ID:SGTC_1994|Compound:st072947|FD-Score:3.30|P-value:4.71E-5 ID:SGTC_1997|Compound:st073024|FD-Score:2.81|P-value:4.37E-4 ID:SGTC_2027|Compound:5150920|FD-Score:3.63|P-value:8.77E-6 ID:SGTC_2029|Compound:5175081|FD-Score:-3.20|P-value:8.86E-5 ID:SGTC_2031|Compound:5180455|FD-Score:-3.12|P-value:1.26E-4 ID:SGTC_2046|Compound:4110841|FD-Score:3.59|P-value:1.10E-5 ID:SGTC_2264|Compound:7948757|FD-Score:-2.69|P-value:8.28E-4 ID:SGTC_2274|Compound:7947094|FD-Score:-2.82|P-value:4.70E-4 ID:SGTC_2304|Compound:7443016|FD-Score:-4.10|P-value:7.43E-7 ID:SGTC_2309|Compound:7664088|FD-Score:3.34|P-value:3.96E-5 ID:SGTC_2319|Compound:6033012|FD-Score:-2.67|P-value:8.94E-4 ID:SGTC_2323|Compound:6148992|FD-Score:-3.23|P-value:7.71E-5 ID:SGTC_2372|Compound:9071633|FD-Score:2.86|P-value:3.63E-4 ID:SGTC_2400|Compound:5203494|FD-Score:-2.97|P-value:2.48E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:2.71|P-value:6.84E-4 ID:SGTC_2564|Compound:betulinic acid|FD-Score:3.73|P-value:5.24E-6 ID:SGTC_2568|Compound:diosmetin|FD-Score:3.88|P-value:2.25E-6 ID:SGTC_2570|Compound:licareol|FD-Score:-4.86|P-value:5.67E-9 ID:SGTC_2575|Compound:plumbagin|FD-Score:2.71|P-value:6.73E-4 ID:SGTC_2579|Compound:2',5'-dihydroxy-4-methoxychalcone|FD-Score:-5.00|P-value:2.23E-9 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:3.64|P-value:8.30E-6 ID:SGTC_2787|Compound:5284540|FD-Score:3.78|P-value:3.98E-6 ID:SGTC_2811|Compound:7967822|FD-Score:2.95|P-value:2.44E-4 ID:SGTC_2815|Compound:7968870|FD-Score:-3.24|P-value:7.45E-5 ID:SGTC_2936|Compound:9038235|FD-Score:-3.23|P-value:7.78E-5 ID:SGTC_2989|Compound:9073649|FD-Score:3.84|P-value:2.81E-6

Top fitness defect scores for YOL125W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1282 0886-0010 4.23 32.4 µM 2.92E-7 endomembrane recycling 28 1.17E-5
2 SGTC_2568 diosmetin 3.88 100.0 µM 2.25E-6 26 5.19E-5
3 SGTC_2989 9073649 3.84 71.4 µM 2.81E-6 45 6.10E-5
4 SGTC_2787 5284540 3.78 71.4 µM 3.98E-6 29 7.86E-5
5 SGTC_2564 betulinic acid 3.73 100.0 µM 5.24E-6 38 9.60E-5
6 SGTC_2621 lithochol-11-enic acid 3.64 100.0 µM 8.30E-6 36 1.34E-4
7 SGTC_2027 5150920 3.63 200.0 µM 8.77E-6 18 1.40E-4
8 SGTC_2046 4110841 3.59 106.0 µM 1.10E-5 iron homeostasis 36 1.65E-4
9 SGTC_865 0488-0499 3.56 301.0 µM 1.29E-5 24 1.85E-4
10 SGTC_1124 fisetin 3.54 1.2 µM 1.43E-5 48 2.00E-4
11 SGTC_2309 7664088 3.34 163.9 µM 3.96E-5 75 4.21E-4
12 SGTC_1994 st072947 3.30 40.9 µM 4.71E-5 38 4.79E-4
13 SGTC_1222 0366-0074 3.30 1.2 µM 4.78E-5 TSC3-RPN4 36 4.84E-4
14 SGTC_1395 3486-0190 3.12 4.3 µM 1.11E-4 49 8.95E-4
15 SGTC_875 0709-0210 2.98 25.7 µM 2.10E-4 fatty acid desaturase (OLE1) 75 0.00143
16 SGTC_2811 7967822 2.95 32.5 µM 2.44E-4 65 0.00160
17 SGTC_1701 st028638 2.90 40.4 µM 3.02E-4 51 0.00187
18 SGTC_859 0439-0102 2.86 54.6 µM 3.51E-4 redox potentiating 63 0.00209
19 SGTC_2372 9071633 2.86 200.0 µM 3.63E-4 57 0.00214
20 SGTC_1997 st073024 2.81 93.8 µM 4.37E-4 43 0.00246

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.170 4.77E-23 YKL130C SHE2 hom RNA-binding protein that binds specific mRNAs and interacts with She3p; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud
0.167 2.34E-22 YDR133C_d YDR133C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR134C
0.134 6.51E-15 YDL131W LYS21 hom Homocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS21 has a paralog, LYS20, that arose from the whole genome duplication
0.134 7.01E-15 YCR088W ABP1 hom Actin-binding protein of the cortical actin cytoskeleton, important for activation of the Arp2/3 complex that plays a key role actin in cytoskeleton organization; phosphorylation within its PRR (Proline-Rich Region), mediated by Cdc28p and Pho85p, protects Abp1p from proteolysis mediated by its own PEST sequences
0.129 5.60E-14 YGL263W COS12 hom Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins
0.129 6.84E-14 YOR302W YOR302W hom CPA1 uORF, Arginine attenuator peptide, regulates translation of the CPA1 mRNA
0.122 1.35E-12 YJR146W_p YJR146W_p hom Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps HMS2
0.118 7.53E-12 YDR312W SSF2 hom Protein required for ribosomal large subunit maturation; functionally redundant with Ssf1p; member of the Brix family; SSF2 has a paralog, SSF1, that arose from the whole genome duplication
0.115 2.25E-11 YGR292W MAL12 hom Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL1 complex locus; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose
0.115 2.75E-11 YBR071W YBR071W hom Protein of unknown function found in the cytoplasm and bud neck; mRNA expression may be regulated by the cell cycle and/or cell wall stress; overexpression of YBR071W affects endocytic protein trafficking
0.114 3.93E-11 YOR301W RAX1 hom Protein involved in bud site selection during bipolar budding; localization requires Rax2p; has similarity to members of the insulin-related peptide superfamily
0.113 4.87E-11 YGR295C COS6 hom Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins
0.110 1.54E-10 YDL157C_p YDL157C_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.110 1.65E-10 YMR308C PSE1 het Karyopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p
0.110 1.93E-10 YJR133W XPT1 hom Xanthine-guanine phosphoribosyl transferase, required for xanthine utilization and for optimal utilization of guanine