YOL151W / GRE2

3-methylbutanal reductase and NADPH-dependent methylglyoxal reductase (D-lactaldehyde dehydrogenase); stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YOL151W / GRE2

Click on Significant Values for Screen Details ID:SGTC_136|Compound:0097-0015|FD-Score:-4.44|P-value:1.08E-7 ID:SGTC_149|Compound:3970-0822|FD-Score:-3.20|P-value:9.41E-5 ID:SGTC_158|Compound:k038-0087|FD-Score:4.23|P-value:3.69E-7 ID:SGTC_630|Compound:4356-0227|FD-Score:3.20|P-value:8.97E-5 ID:SGTC_631|Compound:k072-0230|FD-Score:3.18|P-value:9.94E-5 ID:SGTC_634|Compound:1611-4317|FD-Score:3.11|P-value:1.33E-4 ID:SGTC_911|Compound:3456-4043|FD-Score:3.87|P-value:2.94E-6 ID:SGTC_920|Compound:mycophenolic acid|FD-Score:-3.06|P-value:1.73E-4 ID:SGTC_935|Compound:2996-0153|FD-Score:3.49|P-value:2.16E-5 ID:SGTC_936|Compound:3005-4620|FD-Score:3.17|P-value:1.03E-4 ID:SGTC_1089|Compound:terbinafine|FD-Score:2.96|P-value:2.62E-4 ID:SGTC_1183|Compound:1254-0324|FD-Score:2.73|P-value:7.01E-4 ID:SGTC_1443|Compound:4112-3303|FD-Score:-3.40|P-value:3.60E-5 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.04|P-value:1.94E-4 ID:SGTC_1617|Compound:st002906|FD-Score:-3.12|P-value:1.33E-4 ID:SGTC_1625|Compound:st003711|FD-Score:2.77|P-value:5.79E-4 ID:SGTC_1705|Compound:st027868|FD-Score:3.06|P-value:1.67E-4 ID:SGTC_1709|Compound:st033179|FD-Score:-2.70|P-value:7.98E-4 ID:SGTC_1735|Compound:st034310|FD-Score:-3.42|P-value:3.28E-5 ID:SGTC_1737|Compound:st035557|FD-Score:-2.83|P-value:4.72E-4 ID:SGTC_1739|Compound:st037196|FD-Score:3.22|P-value:7.92E-5 ID:SGTC_1779|Compound:st039427|FD-Score:2.73|P-value:7.01E-4 ID:SGTC_1965|Compound:Pectolinarigenin|FD-Score:-3.01|P-value:2.16E-4 ID:SGTC_2295|Compound:7971645|FD-Score:3.00|P-value:2.18E-4 ID:SGTC_2298|Compound:7976326|FD-Score:3.21|P-value:8.63E-5 ID:SGTC_2324|Compound:6149169|FD-Score:3.12|P-value:1.27E-4 ID:SGTC_2359|Compound:9039622|FD-Score:-2.88|P-value:3.89E-4 ID:SGTC_2571|Compound:chrysanthemic acid|FD-Score:-2.77|P-value:6.03E-4 ID:SGTC_2608|Compound:thymoquinone|FD-Score:3.26|P-value:6.60E-5 ID:SGTC_2670|Compound:beta-carotene|FD-Score:2.70|P-value:7.73E-4 ID:SGTC_2786|Compound:7743617|FD-Score:-2.72|P-value:7.43E-4 ID:SGTC_3008|Compound:9078912|FD-Score:-2.92|P-value:3.22E-4 ID:SGTC_3012|Compound:9080433|FD-Score:4.05|P-value:1.04E-6 ID:SGTC_3302|Compound:9125395|FD-Score:-3.08|P-value:1.58E-4 ID:SGTC_3303|Compound:9125618|FD-Score:3.39|P-value:3.58E-5 ID:SGTC_3306|Compound:9127676|FD-Score:-4.65|P-value:2.77E-8 ID:SGTC_136|Compound:0097-0015|FD-Score:-4.44|P-value:1.08E-7 ID:SGTC_149|Compound:3970-0822|FD-Score:-3.20|P-value:9.41E-5 ID:SGTC_158|Compound:k038-0087|FD-Score:4.23|P-value:3.69E-7 ID:SGTC_630|Compound:4356-0227|FD-Score:3.20|P-value:8.97E-5 ID:SGTC_631|Compound:k072-0230|FD-Score:3.18|P-value:9.94E-5 ID:SGTC_634|Compound:1611-4317|FD-Score:3.11|P-value:1.33E-4 ID:SGTC_911|Compound:3456-4043|FD-Score:3.87|P-value:2.94E-6 ID:SGTC_920|Compound:mycophenolic acid|FD-Score:-3.06|P-value:1.73E-4 ID:SGTC_935|Compound:2996-0153|FD-Score:3.49|P-value:2.16E-5 ID:SGTC_936|Compound:3005-4620|FD-Score:3.17|P-value:1.03E-4 ID:SGTC_1089|Compound:terbinafine|FD-Score:2.96|P-value:2.62E-4 ID:SGTC_1183|Compound:1254-0324|FD-Score:2.73|P-value:7.01E-4 ID:SGTC_1443|Compound:4112-3303|FD-Score:-3.40|P-value:3.60E-5 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.04|P-value:1.94E-4 ID:SGTC_1617|Compound:st002906|FD-Score:-3.12|P-value:1.33E-4 ID:SGTC_1625|Compound:st003711|FD-Score:2.77|P-value:5.79E-4 ID:SGTC_1705|Compound:st027868|FD-Score:3.06|P-value:1.67E-4 ID:SGTC_1709|Compound:st033179|FD-Score:-2.70|P-value:7.98E-4 ID:SGTC_1735|Compound:st034310|FD-Score:-3.42|P-value:3.28E-5 ID:SGTC_1737|Compound:st035557|FD-Score:-2.83|P-value:4.72E-4 ID:SGTC_1739|Compound:st037196|FD-Score:3.22|P-value:7.92E-5 ID:SGTC_1779|Compound:st039427|FD-Score:2.73|P-value:7.01E-4 ID:SGTC_1965|Compound:Pectolinarigenin|FD-Score:-3.01|P-value:2.16E-4 ID:SGTC_2295|Compound:7971645|FD-Score:3.00|P-value:2.18E-4 ID:SGTC_2298|Compound:7976326|FD-Score:3.21|P-value:8.63E-5 ID:SGTC_2324|Compound:6149169|FD-Score:3.12|P-value:1.27E-4 ID:SGTC_2359|Compound:9039622|FD-Score:-2.88|P-value:3.89E-4 ID:SGTC_2571|Compound:chrysanthemic acid|FD-Score:-2.77|P-value:6.03E-4 ID:SGTC_2608|Compound:thymoquinone|FD-Score:3.26|P-value:6.60E-5 ID:SGTC_2670|Compound:beta-carotene|FD-Score:2.70|P-value:7.73E-4 ID:SGTC_2786|Compound:7743617|FD-Score:-2.72|P-value:7.43E-4 ID:SGTC_3008|Compound:9078912|FD-Score:-2.92|P-value:3.22E-4 ID:SGTC_3012|Compound:9080433|FD-Score:4.05|P-value:1.04E-6 ID:SGTC_3302|Compound:9125395|FD-Score:-3.08|P-value:1.58E-4 ID:SGTC_3303|Compound:9125618|FD-Score:3.39|P-value:3.58E-5 ID:SGTC_3306|Compound:9127676|FD-Score:-4.65|P-value:2.77E-8

Top fitness defect scores for YOL151W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_158 k038-0087 4.23 26.4 µM 3.69E-7 ubiquinone biosynthesis & proteasome 124 1.18E-5
2 SGTC_3012 9080433 4.05 71.4 µM 1.04E-6 11 2.53E-5
3 SGTC_911 3456-4043 3.87 236.0 µM 2.94E-6 mitochondrial processes 29 5.48E-5
4 SGTC_935 2996-0153 3.49 430.0 µM 2.16E-5 PDR1 33 2.40E-4
5 SGTC_3303 9125618 3.39 40.6 µM 3.58E-5 67 3.49E-4
6 SGTC_2608 thymoquinone 3.26 42.7 µM 6.60E-5 37 5.50E-4
7 SGTC_1739 st037196 3.22 92.5 µM 7.92E-5 54 6.30E-4
8 SGTC_2298 7976326 3.21 193.8 µM 8.63E-5 24 6.72E-4
9 SGTC_630 4356-0227 3.20 183.0 µM 8.97E-5 27 6.92E-4
10 SGTC_631 k072-0230 3.18 41.5 µM 9.94E-5 60S ribosome export 51 7.46E-4
11 SGTC_936 3005-4620 3.17 401.0 µM 1.03E-4 63 7.66E-4
12 SGTC_2324 6149169 3.12 200.0 µM 1.27E-4 41 8.94E-4
13 SGTC_634 1611-4317 3.11 11.2 µM 1.33E-4 ERG2 45 9.29E-4
14 SGTC_1705 st027868 3.06 56.3 µM 1.67E-4 37 0.00110
15 SGTC_2295 7971645 3.00 200.0 µM 2.18E-4 62 0.00134
16 SGTC_1089 terbinafine 2.96 2.2 µM 2.62E-4 55 0.00154
17 SGTC_1625 st003711 2.77 30.1 µM 5.79E-4 calcium & mitochondrial duress 108 0.00278
18 SGTC_1779 st039427 2.73 55.0 µM 7.01E-4 118 0.00320
19 SGTC_1183 1254-0324 2.73 198.0 µM 7.01E-4 80 0.00320
20 SGTC_2670 beta-carotene 2.70 100.0 µM 7.73E-4 23 0.00345

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.126 2.21E-13 YLR201C COQ9 hom Protein required for ubiquinone (coenzyme Q) biosynthesis and respiratory growth; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes
0.120 3.29E-12 YPL166W ATG29 hom Autophagy-specific protein; required for recruiting other ATG proteins to the pre-autophagosomal structure (PAS); interacts with Atg17p and localizas to the PAS in a manner interdependent with Atg17p and Cis1p; not conserved; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
0.120 3.49E-12 YPR064W_d YPR064W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.118 6.45E-12 YOL008W COQ10 hom Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q6 to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes
0.110 1.74E-10 YIL085C KTR7 hom Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR7 has a paralog, KTR5, that arose from the whole genome duplication
0.102 2.95E-9 YER118C SHO1 hom Transmembrane osmosensor involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p
0.101 4.37E-9 YJL007C_d YJL007C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.100 7.09E-9 YGR255C COQ6 hom Putative flavin-dependent monooxygenase; involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; human COX6 can rescue a yeast cox6 mutant and is implicated in steroid-resistant nephrotic syndrome (SRNS)
0.097 1.73E-8 YCL014W BUD3 hom Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding
0.096 2.38E-8 YOR028C CIN5 hom Basic leucine zipper (bZIP) transcription factor of the yAP-1 family; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; mediates pleiotropic drug resistance and salt tolerance; nuclearly localized under oxidative stress and sequestered in the cytoplasm by Lot6p under reducing conditions; CIN5 has a paralog, YAP6, that arose from the whole genome duplication
0.095 3.57E-8 YOR254C SEC63 het Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
0.095 3.61E-8 YLL051C FRE6 hom Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels
0.093 7.20E-8 YAL015C NTG1 hom DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication
0.091 1.12E-7 YMR127C SAS2 hom Histone acetyltransferase (HAT) catalytic subunit of the SAS complex (Sas2p-Sas4p-Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; member of the MYSTacetyltransferase family
0.090 1.77E-7 YHR001W OSH7 hom Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability