HIPHOP chemogenomics database

YOR001W / RRP6

Nuclear exosome exonuclease component; has 3'-5' exonuclease activity; involved in RNA processing, maturation, surveillance, degradation, tethering, and export; has similarity to E. coli RNase D and to human PM-Sc1 100 (EXOSC10); mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay

Zygosity: Homozygous strain

fixedexpanded
Profile for YOR001W / RRP6

Click on Significant Values for Screen Details

Top fitness defect scores for YOR001W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_3337	9144342	8.39	33.5 µM	4.37E-22		8	2.42E-17
2	SGTC_3148	9097893	7.49	49.5 µM	4.70E-18		7	3.36E-14
3	SGTC_2932	9016711	7.19	44.5 µM	8.67E-17		10	3.26E-13
4	SGTC_241	5hr heat shock (37°C) + cantharidin	6.53	155.4 µM	3.23E-14	cell wall signaling	27	3.27E-11
5	SGTC_240	5hr heat shock (37°C) + Streptovitacin	5.86	300.0 µM	7.90E-12	heat shock/prefoldin	18	2.35E-9
6	SGTC_239	5hr heat shock (37°C) + mitomycin C	4.96	819.4 µM	4.87E-9	heat shock/prefoldin	19	3.46E-7
7	SGTC_582	0987-0078	4.61	156.0 µM	4.63E-8	calcium & mitochondrial duress	42	1.98E-6
8	SGTC_238	5hr heat shock (37°C) + nsc-638432	4.41	8.9 µM	1.62E-7	heat shock/prefoldin	28	5.23E-6
9	SGTC_2603	homopterocarpin	4.24	100.0 µM	4.35E-7		25	1.13E-5
10	SGTC_2933	9026794	4.16	32.5 µM	6.61E-7		12	1.56E-5
11	SGTC_243	5hr heat shock (37°C) + splitomicin	4.16	1.0 µM	6.90E-7	heat shock/prefoldin	46	1.61E-5
12	SGTC_242	5hr heat shock (37°C) + 5-fluorouracil	4.13	4.6 µM	8.14E-7	heat shock/prefoldin	32	1.83E-5
13	SGTC_101	0490-5701	4.12	117.5 µM	8.55E-7		11	1.90E-5
14	SGTC_1792	st045773	3.91	43.6 µM	2.73E-6		27	4.66E-5
15	SGTC_1137	ixabepilone	3.79	100.0 µM	5.10E-6		32	7.57E-5
16	SGTC_3240	9133527	3.64	49.5 µM	1.10E-5		17	1.38E-4
17	SGTC_667	1498-1044	3.56	59.0 µM	1.60E-5	RPP1 & pyrimidine depletion	29	1.83E-4
18	SGTC_2325	6158266	3.45	200.0 µM	2.81E-5		43	2.84E-4
19	SGTC_2057	5236571	3.24	38.3 µM	7.30E-5	RPP1 & pyrimidine depletion	70	5.94E-4
20	SGTC_2881	9058953	3.22	52.0 µM	8.21E-5		51	6.50E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.359	1.78E-102	YLR027C	AAT2	hom	Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells
0.348	6.11E-96	YDR457W	TOM1	hom	E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase
0.330	4.70E-86	YDR260C	SWM1	hom	Subunit of the anaphase-promoting complex, which is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore wall maturation
0.284	2.09E-63	YCR063W	BUD31	hom	Component of the SF3b subcomplex of the U2 snRNP; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis
0.279	9.36E-61	YNL140C_p	YNL140C_p	hom	Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps THO2/YNL139C
0.219	1.21E-37	YLR200W	YKE2	hom	Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin
0.200	2.01E-31	YDR433W_d	YDR433W_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.192	3.18E-29	YDR069C	DOA4	hom	Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole
0.172	8.79E-24	YNL098C	RAS2	hom	GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes
0.169	8.57E-23	YHR041C	SRB2	hom	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance
0.165	7.18E-22	YHR209W	CRG1	hom	S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin
0.163	2.68E-21	YCR077C	PAT1	hom	Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress
0.152	8.77E-19	YKR024C	DBP7	hom	Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions
0.149	4.11E-18	YDR392W	SPT3	hom	Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters
0.145	2.56E-17	YLL045C	RPL8B	hom	Ribosomal 60S subunit protein L8B; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8B has a paralog, RPL8A, that arose from the whole genome duplication