YOR197W / MCA1

Ca2+-dependent cysteine protease; may cleave specific substrates during the stress response; regulates apoptosis upon H2O2 treatment; required for clearance of insoluble protein aggregates during normal growth; implicated in cell cycle dynamics; undergoes autocatalytic processing; similar to mammalian metacaspases, but exists as a monomer due to an extra pair of anti-parallel beta-strands that form a continuous beta-sheet, blocking potential dimerization

Zygosity: Homozygous strain
fixedexpanded
Profile for YOR197W / MCA1

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:3.36|P-value:1.30E-4 ID:SGTC_20|Compound:0481-0013|FD-Score:-2.85|P-value:9.50E-4 ID:SGTC_42|Compound:0133-0086|FD-Score:-3.77|P-value:2.00E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:3.25|P-value:2.07E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:3.51|P-value:6.67E-5 ID:SGTC_117|Compound:lucigenin|FD-Score:5.31|P-value:3.69E-9 ID:SGTC_266|Compound:formosanin C|FD-Score:4.61|P-value:2.69E-7 ID:SGTC_331|Compound:k048-0133|FD-Score:2.86|P-value:9.29E-4 ID:SGTC_350|Compound:k064-0020|FD-Score:3.50|P-value:6.94E-5 ID:SGTC_428|Compound:3448-4153|FD-Score:-4.43|P-value:6.93E-7 ID:SGTC_444|Compound:curcumin|FD-Score:2.86|P-value:9.44E-4 ID:SGTC_472|Compound:calcifediol|FD-Score:3.43|P-value:9.39E-5 ID:SGTC_482|Compound:fipronil|FD-Score:3.20|P-value:2.51E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:4.09|P-value:4.36E-6 ID:SGTC_669|Compound:0108-0021|FD-Score:3.55|P-value:5.52E-5 ID:SGTC_769|Compound:0302-0231|FD-Score:2.89|P-value:8.37E-4 ID:SGTC_817|Compound:1391-0715|FD-Score:3.42|P-value:1.01E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:3.96|P-value:8.10E-6 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.16|P-value:2.83E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:4.29|P-value:1.49E-6 ID:SGTC_1196|Compound:1588-0294|FD-Score:3.06|P-value:4.36E-4 ID:SGTC_1288|Compound:0973-0013|FD-Score:3.09|P-value:3.87E-4 ID:SGTC_1459|Compound:k035-0031|FD-Score:-4.10|P-value:3.83E-6 ID:SGTC_1470|Compound:k295-0292|FD-Score:3.21|P-value:2.37E-4 ID:SGTC_1530|Compound:rhein|FD-Score:3.64|P-value:3.75E-5 ID:SGTC_1726|Compound:st035556|FD-Score:3.28|P-value:1.77E-4 ID:SGTC_1770|Compound:st043884|FD-Score:-3.22|P-value:2.20E-4 ID:SGTC_1840|Compound:st055363|FD-Score:-3.17|P-value:2.69E-4 ID:SGTC_1978|Compound:st071761|FD-Score:-3.06|P-value:4.16E-4 ID:SGTC_1982|Compound:st072467|FD-Score:3.28|P-value:1.78E-4 ID:SGTC_1983|Compound:st071813|FD-Score:-3.10|P-value:3.63E-4 ID:SGTC_2005|Compound:4024620|FD-Score:-2.93|P-value:7.02E-4 ID:SGTC_2065|Compound:5221596|FD-Score:6.05|P-value:2.25E-11 ID:SGTC_2067|Compound:5224629|FD-Score:2.88|P-value:8.74E-4 ID:SGTC_2241|Compound:6772625|FD-Score:-3.99|P-value:6.66E-6 ID:SGTC_2308|Compound:7584454|FD-Score:3.64|P-value:3.80E-5 ID:SGTC_2347|Compound:9049783|FD-Score:2.92|P-value:7.36E-4 ID:SGTC_2496|Compound:diffractaic acid|FD-Score:3.28|P-value:1.83E-4 ID:SGTC_2590|Compound:3,4',5',6',7'-pentamethoxyflavone|FD-Score:-2.84|P-value:9.80E-4 ID:SGTC_2693|Compound:st077581|FD-Score:5.38|P-value:2.37E-9 ID:SGTC_2714|Compound:st079313|FD-Score:-3.16|P-value:2.86E-4 ID:SGTC_2716|Compound:st080858|FD-Score:3.98|P-value:7.48E-6 ID:SGTC_2800|Compound:7887935|FD-Score:2.99|P-value:5.69E-4 ID:SGTC_2862|Compound:9027634|FD-Score:4.85|P-value:6.58E-8 ID:SGTC_2958|Compound:9077254|FD-Score:2.85|P-value:9.86E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:3.36|P-value:1.30E-4 ID:SGTC_20|Compound:0481-0013|FD-Score:-2.85|P-value:9.50E-4 ID:SGTC_42|Compound:0133-0086|FD-Score:-3.77|P-value:2.00E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:3.25|P-value:2.07E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:3.51|P-value:6.67E-5 ID:SGTC_117|Compound:lucigenin|FD-Score:5.31|P-value:3.69E-9 ID:SGTC_266|Compound:formosanin C|FD-Score:4.61|P-value:2.69E-7 ID:SGTC_331|Compound:k048-0133|FD-Score:2.86|P-value:9.29E-4 ID:SGTC_350|Compound:k064-0020|FD-Score:3.50|P-value:6.94E-5 ID:SGTC_428|Compound:3448-4153|FD-Score:-4.43|P-value:6.93E-7 ID:SGTC_444|Compound:curcumin|FD-Score:2.86|P-value:9.44E-4 ID:SGTC_472|Compound:calcifediol|FD-Score:3.43|P-value:9.39E-5 ID:SGTC_482|Compound:fipronil|FD-Score:3.20|P-value:2.51E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:4.09|P-value:4.36E-6 ID:SGTC_669|Compound:0108-0021|FD-Score:3.55|P-value:5.52E-5 ID:SGTC_769|Compound:0302-0231|FD-Score:2.89|P-value:8.37E-4 ID:SGTC_817|Compound:1391-0715|FD-Score:3.42|P-value:1.01E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:3.96|P-value:8.10E-6 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.16|P-value:2.83E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:4.29|P-value:1.49E-6 ID:SGTC_1196|Compound:1588-0294|FD-Score:3.06|P-value:4.36E-4 ID:SGTC_1288|Compound:0973-0013|FD-Score:3.09|P-value:3.87E-4 ID:SGTC_1459|Compound:k035-0031|FD-Score:-4.10|P-value:3.83E-6 ID:SGTC_1470|Compound:k295-0292|FD-Score:3.21|P-value:2.37E-4 ID:SGTC_1530|Compound:rhein|FD-Score:3.64|P-value:3.75E-5 ID:SGTC_1726|Compound:st035556|FD-Score:3.28|P-value:1.77E-4 ID:SGTC_1770|Compound:st043884|FD-Score:-3.22|P-value:2.20E-4 ID:SGTC_1840|Compound:st055363|FD-Score:-3.17|P-value:2.69E-4 ID:SGTC_1978|Compound:st071761|FD-Score:-3.06|P-value:4.16E-4 ID:SGTC_1982|Compound:st072467|FD-Score:3.28|P-value:1.78E-4 ID:SGTC_1983|Compound:st071813|FD-Score:-3.10|P-value:3.63E-4 ID:SGTC_2005|Compound:4024620|FD-Score:-2.93|P-value:7.02E-4 ID:SGTC_2065|Compound:5221596|FD-Score:6.05|P-value:2.25E-11 ID:SGTC_2067|Compound:5224629|FD-Score:2.88|P-value:8.74E-4 ID:SGTC_2241|Compound:6772625|FD-Score:-3.99|P-value:6.66E-6 ID:SGTC_2308|Compound:7584454|FD-Score:3.64|P-value:3.80E-5 ID:SGTC_2347|Compound:9049783|FD-Score:2.92|P-value:7.36E-4 ID:SGTC_2496|Compound:diffractaic acid|FD-Score:3.28|P-value:1.83E-4 ID:SGTC_2590|Compound:3,4',5',6',7'-pentamethoxyflavone|FD-Score:-2.84|P-value:9.80E-4 ID:SGTC_2693|Compound:st077581|FD-Score:5.38|P-value:2.37E-9 ID:SGTC_2714|Compound:st079313|FD-Score:-3.16|P-value:2.86E-4 ID:SGTC_2716|Compound:st080858|FD-Score:3.98|P-value:7.48E-6 ID:SGTC_2800|Compound:7887935|FD-Score:2.99|P-value:5.69E-4 ID:SGTC_2862|Compound:9027634|FD-Score:4.85|P-value:6.58E-8 ID:SGTC_2958|Compound:9077254|FD-Score:2.85|P-value:9.86E-4

Top fitness defect scores for YOR197W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2065 5221596 6.05 34.3 µM 2.25E-11 1 7.20E-10
2 SGTC_2693 st077581 5.38 40.7 µM 2.37E-9 9 3.72E-8
3 SGTC_117 lucigenin 5.31 22.1 µM 3.69E-9 redox potentiating 53 5.41E-8
4 SGTC_2862 9027634 4.85 71.4 µM 6.58E-8 ERG2 16 6.22E-7
5 SGTC_266 formosanin C 4.61 406.0 nM 2.69E-7 sphingolipid biosynthesis & PDR1 53 2.05E-6
6 SGTC_1161 k007-0610 4.29 110.0 µM 1.49E-6 ERAD & cell cycle 107 8.77E-6
7 SGTC_568 1193-0097 4.09 7.5 µM 4.36E-6 ERAD & cell cycle 138 2.19E-5
8 SGTC_2716 st080858 3.98 38.6 µM 7.48E-6 60S ribosome export 19 3.46E-5
9 SGTC_916 4466-0038 3.96 42.0 nM 8.10E-6 cell wall 70 3.70E-5
10 SGTC_1530 rhein 3.64 70.4 µM 3.75E-5 27 1.36E-4
11 SGTC_2308 7584454 3.64 197.6 µM 3.80E-5 34 1.38E-4
12 SGTC_669 0108-0021 3.55 26.2 µM 5.52E-5 fatty acid desaturase (OLE1) 108 1.90E-4
13 SGTC_103 0844-0104 3.51 51.8 µM 6.67E-5 ERAD & cell cycle 167 2.23E-4
14 SGTC_350 k064-0020 3.50 19.8 µM 6.94E-5 heme requiring 38 2.30E-4
15 SGTC_472 calcifediol 3.43 10.0 µM 9.39E-5 14 2.98E-4
16 SGTC_817 1391-0715 3.42 46.0 µM 1.01E-4 30 3.16E-4
17 SGTC_6 0844-0013 3.36 19.8 µM 1.30E-4 156 3.93E-4
18 SGTC_1726 st035556 3.28 14.6 µM 1.77E-4 69 5.10E-4
19 SGTC_1982 st072467 3.28 71.9 µM 1.78E-4 64 5.14E-4
20 SGTC_2496 diffractaic acid 3.28 79.8 µM 1.83E-4 44 5.26E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.214 4.39E-36 YDL148C NOP14 het Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA
0.205 2.51E-33 YOL065C INP54 hom Phosphatidylinositol 4,5-bisphosphate 5-phosphatase with a role in secretion, localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins
0.155 1.28E-19 YLR407W_p YLR407W_p hom Putative protein of unknown function; null mutant displays elongated buds and a large fraction of budded cells have only one nucleus
0.153 4.22E-19 YMR116C ASC1 hom G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; regulates P-body formation induced by replication stress; represses Gcn4p in the absence of amino acid starvation
0.153 5.19E-19 YMR133W REC114 hom Protein involved in early stages of meiotic recombination; possibly involved in the coordination of recombination and meiotic division; mutations lead to premature initiation of the first meiotic division
0.150 1.96E-18 YDR186C YDR186C hom Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.149 3.89E-18 YLR412W BER1 hom Protein involved in microtubule-related processes, N-acetylation; GFP-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YLR412W is not an essential gene; similar to Arabidopsis SRR1 gene
0.148 7.81E-18 YBL066C SEF1 hom Putative transcription factor, has homolog in Kluyveromyces lactis
0.146 2.05E-17 YGL116W CDC20 het Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
0.145 3.30E-17 YER084W_p YER084W_p hom Protein of unknown function; expressed at both mRNA and protein levels
0.143 9.05E-17 YJR092W BUD4 hom Anillin-like protein involved in bud-site selection; required for the axial budding pattern; localizes with septins to the bud neck in mitosis and may constitute an axial landmark for the next round of budding; required for the formation and disassembly of the double septin ring structure, and generally for septin organization; in vivo substrate of Cdc28p/Clb2p
0.142 1.38E-16 YCL076W_d YCL076W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.142 1.54E-16 YDR199W_d YDR199W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64; computationally predicted to have thiol-disulfide oxidoreductase activity
0.142 1.53E-16 YDR057W YOS9 hom ER quality-control lectin; integral subunit of the HRD ligase; binds to glycans with terminal alpha-1,6 linked mannose on misfolded N-glycosylated proteins and participates in targeting proteins to ERAD; member of the OS-9 protein family
0.137 1.95E-15 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate