YOR227W / HER1

Protein of unknown function required for proliferation or remodeling of the ER that is caused by overexpression of Hmg2p; may interact with ribosomes, based on co-purification experiments; HER1 has a paralog, GIP3, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YOR227W / HER1

Click on Significant Values for Screen Details ID:SGTC_191|Compound:3914-0123|FD-Score:3.21|P-value:9.35E-4 ID:SGTC_413|Compound:propylparaben|FD-Score:-3.22|P-value:8.62E-4 ID:SGTC_429|Compound:1112-0049|FD-Score:5.10|P-value:3.77E-7 ID:SGTC_540|Compound:1187-0821|FD-Score:4.14|P-value:3.05E-5 ID:SGTC_553|Compound:k007-0256|FD-Score:4.36|P-value:1.19E-5 ID:SGTC_560|Compound:2332-1943|FD-Score:3.36|P-value:5.64E-4 ID:SGTC_980|Compound:1473-0395|FD-Score:4.31|P-value:1.49E-5 ID:SGTC_1010|Compound:4112-3296|FD-Score:3.23|P-value:8.63E-4 ID:SGTC_1076|Compound:itraconazole|FD-Score:4.36|P-value:1.18E-5 ID:SGTC_1244|Compound:0416-0015|FD-Score:4.34|P-value:1.30E-5 ID:SGTC_1677|Compound:st019204|FD-Score:3.84|P-value:9.77E-5 ID:SGTC_2269|Compound:7935389|FD-Score:-3.36|P-value:5.48E-4 ID:SGTC_2578|Compound:7,8-dihydroxyflavone|FD-Score:3.60|P-value:2.41E-4 ID:SGTC_2795|Compound:7780010|FD-Score:4.04|P-value:4.49E-5 ID:SGTC_2799|Compound:7812892|FD-Score:3.28|P-value:7.49E-4 ID:SGTC_3316|Compound:9136639|FD-Score:3.54|P-value:3.03E-4 ID:SGTC_191|Compound:3914-0123|FD-Score:3.21|P-value:9.35E-4 ID:SGTC_413|Compound:propylparaben|FD-Score:-3.22|P-value:8.62E-4 ID:SGTC_429|Compound:1112-0049|FD-Score:5.10|P-value:3.77E-7 ID:SGTC_540|Compound:1187-0821|FD-Score:4.14|P-value:3.05E-5 ID:SGTC_553|Compound:k007-0256|FD-Score:4.36|P-value:1.19E-5 ID:SGTC_560|Compound:2332-1943|FD-Score:3.36|P-value:5.64E-4 ID:SGTC_980|Compound:1473-0395|FD-Score:4.31|P-value:1.49E-5 ID:SGTC_1010|Compound:4112-3296|FD-Score:3.23|P-value:8.63E-4 ID:SGTC_1076|Compound:itraconazole|FD-Score:4.36|P-value:1.18E-5 ID:SGTC_1244|Compound:0416-0015|FD-Score:4.34|P-value:1.30E-5 ID:SGTC_1677|Compound:st019204|FD-Score:3.84|P-value:9.77E-5 ID:SGTC_2269|Compound:7935389|FD-Score:-3.36|P-value:5.48E-4 ID:SGTC_2578|Compound:7,8-dihydroxyflavone|FD-Score:3.60|P-value:2.41E-4 ID:SGTC_2795|Compound:7780010|FD-Score:4.04|P-value:4.49E-5 ID:SGTC_2799|Compound:7812892|FD-Score:3.28|P-value:7.49E-4 ID:SGTC_3316|Compound:9136639|FD-Score:3.54|P-value:3.03E-4

Top fitness defect scores for YOR227W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_429 1112-0049 5.10 25.5 µM 3.77E-7 DNA damage response 19 1.68E-7
2 SGTC_1076 itraconazole 4.36 331.0 nM 1.18E-5 24 6.48E-6
3 SGTC_553 k007-0256 4.36 45.7 µM 1.19E-5 heme biosynthesis & mitochondrial translocase 14 6.55E-6
4 SGTC_1244 0416-0015 4.34 52.5 µM 1.30E-5 ERG2 22 7.14E-6
5 SGTC_980 1473-0395 4.31 201.0 µM 1.49E-5 6 8.27E-6
6 SGTC_540 1187-0821 4.14 17.3 µM 3.05E-5 12 1.77E-5
7 SGTC_2795 7780010 4.04 54.5 µM 4.49E-5 26 2.67E-5
8 SGTC_1677 st019204 3.84 53.9 µM 9.77E-5 20 6.08E-5
9 SGTC_2578 7,8-dihydroxyflavone 3.60 64.4 µM 2.41E-4 40 1.58E-4
10 SGTC_3316 9136639 3.54 10.6 µM 3.03E-4 21 2.01E-4
11 SGTC_560 2332-1943 3.36 155.0 µM 5.64E-4 25 3.89E-4
12 SGTC_2799 7812892 3.28 81.8 µM 7.49E-4 superoxide 54 5.26E-4
13 SGTC_1010 4112-3296 3.23 15.2 µM 8.63E-4 44 6.10E-4
14 SGTC_191 3914-0123 3.21 66.5 µM 9.35E-4 60S ribosome export 6 6.64E-4
15 SGTC_1829 berberine 2.99 14.4 µM 0.00187 60S ribosome export 55 0.00138
16 SGTC_1180 1193-0155 2.94 285.0 µM 0.00218 58 0.00162
17 SGTC_1787 fluconazole 2.94 80.0 µM 0.00221 iron homeostasis 97 0.00165
18 SGTC_228 caffeine 2.94 993.1 µM 0.00221 37 0.00165
19 SGTC_1074 carmofur 2.93 3.8 µM 0.00227 exosome 80 0.00170
20 SGTC_370 0180-0345 2.92 38.7 µM 0.00233 DNA intercalators 46 0.00175

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.136 3.04E-15 YMR306C-A_d YMR306C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.125 4.45E-13 YPL190C NAB3 het RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; required for termination of non-poly(A) transcripts and efficient splicing
0.117 9.63E-12 YOR293C-A_p YOR293C-A_p hom Putative protein of unknown function; identified by expression profiling and mass spectrometry
0.115 2.26E-11 YOR259C RPT4 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization
0.103 2.03E-9 YDR341C YDR341C het Arginyl-tRNA synthetase, proposed to be cytoplasmic but the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.099 7.94E-9 YGL087C MMS2 hom Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress
0.098 1.24E-8 YJL217W REE1 hom Cytoplasmic protein involved in the regulation of enolase (ENO1); mRNA expression is induced by calcium shortage, copper deficiency (via Mac1p) and the presence of galactose (via Gal4p); mRNA expression is also regulated by the cell cycle
0.098 1.45E-8 YOR289W_p YOR289W_p hom Putative protein of unknown function; transcription induced by the unfolded protein response; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
0.098 1.45E-8 YIR029W DAL2 hom Allantoicase, converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation
0.097 2.11E-8 YPL256C CLN2 hom G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p)
0.092 8.34E-8 YLR356W ATG33 hom Mitochondrial mitophagy-specific protein; required primarily for mitophagy induced at the post-log phase; not required for other types of selective autophagy or macroautophagy; conserved within fungi, but not in higher eukaryotes
0.089 2.18E-7 YFL030W AGX1 hom Alanine:glyoxylate aminotransferase (AGT), catalyzes the synthesis of glycine from glyoxylate, which is one of three pathways for glycine biosynthesis in yeast; has similarity to mammalian and plant alanine:glyoxylate aminotransferases
0.087 5.03E-7 YOL022C TSR4 het Cytoplasmic protein required for correct processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; essential gene in S288C background but not in CEN.PK2
0.086 5.66E-7 YKL195W MIA40 het Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p
0.085 9.40E-7 YBR025C OLA1 hom P-loop ATPase with similarity to human OLA1 and bacterial YchF; identified as specifically interacting with the proteasome; protein abundance increases in response to hydrogen peroxide and to DNA replication stress