HIPHOP chemogenomics database

YOR262W / GPN2

Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion

Zygosity: Heterozygous strain

fixedexpanded
Profile for YOR262W / GPN2

Click on Significant Values for Screen Details

Top fitness defect scores for YOR262W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_2004	4023728	12.20	41.9 µM	3.65E-33		5	1.82E-34	1.70
2	SGTC_1000	4048-0884	9.08	8.7 µM	2.72E-19	copper-dependent oxidative stress	1	5.19E-20	1.83
3	SGTC_1069	diphenylcyclopropenone	9.05	8.5 µM	3.62E-19		3	6.99E-20	3.36
4	SGTC_2921	7974240	8.33	10.7 µM	1.56E-16		3	3.88E-17	1.48
5	SGTC_1367	1812-0510	7.87	76.9 µM	6.26E-15		3	1.81E-15	3.31
6	SGTC_820	1582-0060	7.13	59.9 µM	1.42E-12		4	5.16E-13	0.08
7	SGTC_2090	5301565	6.27	104.5 µM	4.04E-10	TSC3-RPN4	2	1.85E-10	1.30
8	SGTC_611	3453-1652	6.07	23.7 µM	1.31E-9		8	6.30E-10	0.19
9	SGTC_3143	9086658	5.89	49.5 µM	3.86E-9		4	1.94E-9	0.78
10	SGTC_2534	helenine	5.69	20.1 µM	1.24E-8	RSC complex & mRNA processing	14	6.49E-9	0.17
11	SGTC_2233	sulbentine	5.53	40.4 µM	2.92E-8		24	1.59E-8	0.13
12	SGTC_1773	st048542	5.43	73.6 µM	4.98E-8		13	2.77E-8	0.13
13	SGTC_3211	9128781	4.94	49.5 µM	6.27E-7		10	3.86E-7	0.42
14	SGTC_1585	2',4'-dihydroxychalcone	4.92	10.4 µM	6.83E-7		5	4.22E-7	0.01
15	SGTC_630	4356-0227	4.88	183.0 µM	8.42E-7		2	5.25E-7	0.45
16	SGTC_2093	5331342	4.74	194.4 µM	1.69E-6		3	1.08E-6	0.70
17	SGTC_897	1000-0139	4.60	31.4 µM	3.19E-6		8	2.10E-6	0.69
18	SGTC_2510	benzyl isothiocyanate	4.58	8.3 µM	3.52E-6		17	2.32E-6	0.05
19	SGTC_879	0831-1030	4.50	162.0 µM	5.10E-6		16	3.41E-6	0.01
20	SGTC_428	3448-4153	4.49	16.5 µM	5.36E-6	TSC3-RPN4	7	3.60E-6	0.40

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.458	2.37E-173	YDR527W	RBA50	het	Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1
0.339	3.42E-91	YMR185W	RTP1	het	Protein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene
0.282	2.42E-62	YLR243W	GPN3	het	Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn2p
0.216	8.66E-37	YOR207C	RET1	het	Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
0.195	3.94E-30	YAL024C	LTE1	hom	Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures
0.194	7.04E-30	YPR085C	ASA1	het	Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1
0.184	7.31E-27	YOR349W	CIN1	hom	Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl
0.171	1.70E-23	YML124C	TUB3	hom	Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication
0.166	3.38E-22	YNL148C	ALF1	hom	Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance
0.145	3.51E-17	YER107C	GLE2	hom	RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1
0.142	1.73E-16	YNL298W	CLA4	hom	Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p
0.133	1.17E-14	YBR058C-A	TSC3	hom	Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
0.132	1.83E-14	YPL241C	CIN2	hom	GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2
0.123	7.61E-13	YNL216W	RAP1	het	Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain)
0.117	1.24E-11	YFL023W	BUD27	hom	Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP