YOR303W / CPA1

Small subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor; translationally regulated by an attenuator peptide encoded by YOR302W within the CPA1 mRNA 5'-leader

Zygosity: Homozygous strain
fixedexpanded
Profile for YOR303W / CPA1

Click on Significant Values for Screen Details ID:SGTC_19|Compound:0279-0214|FD-Score:3.29|P-value:2.63E-4 ID:SGTC_54|Compound:0159-0055|FD-Score:7.00|P-value:1.13E-13 ID:SGTC_299|Compound:1273-0059|FD-Score:3.24|P-value:3.19E-4 ID:SGTC_303|Compound:1348-1668|FD-Score:-3.29|P-value:3.51E-4 ID:SGTC_521|Compound:r062-0005|FD-Score:-3.17|P-value:5.49E-4 ID:SGTC_524|Compound:r073-0010|FD-Score:-3.49|P-value:1.61E-4 ID:SGTC_530|Compound:4358-1714|FD-Score:3.60|P-value:7.42E-5 ID:SGTC_537|Compound:1334-0398|FD-Score:3.08|P-value:5.86E-4 ID:SGTC_669|Compound:0108-0021|FD-Score:4.80|P-value:2.30E-7 ID:SGTC_716|Compound:1683-5115|FD-Score:3.35|P-value:2.11E-4 ID:SGTC_815|Compound:1582-0027|FD-Score:-5.28|P-value:2.36E-8 ID:SGTC_1131|Compound:3454-3128|FD-Score:3.15|P-value:4.44E-4 ID:SGTC_1245|Compound:0418-0076|FD-Score:3.56|P-value:8.88E-5 ID:SGTC_1263|Compound:0774-1208|FD-Score:2.96|P-value:8.78E-4 ID:SGTC_1286|Compound:0929-0070|FD-Score:3.69|P-value:5.08E-5 ID:SGTC_1480|Compound:1070-0044|FD-Score:-3.44|P-value:1.93E-4 ID:SGTC_1580|Compound:hydrocortisone 21-hemisuccinate|FD-Score:-3.92|P-value:2.62E-5 ID:SGTC_1630|Compound:st005209|FD-Score:5.17|P-value:2.81E-8 ID:SGTC_1780|Compound:st038448|FD-Score:3.72|P-value:4.48E-5 ID:SGTC_1781|Compound:st038725|FD-Score:-4.34|P-value:3.70E-6 ID:SGTC_1848|Compound:st056003|FD-Score:-4.50|P-value:1.67E-6 ID:SGTC_1851|Compound:st056236|FD-Score:3.90|P-value:2.00E-5 ID:SGTC_1857|Compound:st056007|FD-Score:-4.18|P-value:7.98E-6 ID:SGTC_1910|Compound:st062352|FD-Score:-3.12|P-value:6.50E-4 ID:SGTC_1992|Compound:st073643|FD-Score:-3.43|P-value:2.06E-4 ID:SGTC_2175|Compound:5807670|FD-Score:3.01|P-value:7.39E-4 ID:SGTC_2220|Compound:6782180|FD-Score:3.27|P-value:2.82E-4 ID:SGTC_2329|Compound:6138490|FD-Score:2.96|P-value:8.95E-4 ID:SGTC_2407|Compound:paclitaxel|FD-Score:4.11|P-value:7.79E-6 ID:SGTC_2616|Compound:abietic acid|FD-Score:4.41|P-value:1.76E-6 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:-3.85|P-value:3.50E-5 ID:SGTC_2787|Compound:5284540|FD-Score:-3.45|P-value:1.85E-4 ID:SGTC_2901|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:-3.55|P-value:1.25E-4 ID:SGTC_2903|Compound:5923464|FD-Score:-4.21|P-value:6.85E-6 ID:SGTC_2928|Compound:9011484|FD-Score:5.22|P-value:2.16E-8 ID:SGTC_2967|Compound:9089538|FD-Score:3.25|P-value:3.00E-4 ID:SGTC_3326|Compound:9140064|FD-Score:-3.68|P-value:7.27E-5 ID:SGTC_19|Compound:0279-0214|FD-Score:3.29|P-value:2.63E-4 ID:SGTC_54|Compound:0159-0055|FD-Score:7.00|P-value:1.13E-13 ID:SGTC_299|Compound:1273-0059|FD-Score:3.24|P-value:3.19E-4 ID:SGTC_303|Compound:1348-1668|FD-Score:-3.29|P-value:3.51E-4 ID:SGTC_521|Compound:r062-0005|FD-Score:-3.17|P-value:5.49E-4 ID:SGTC_524|Compound:r073-0010|FD-Score:-3.49|P-value:1.61E-4 ID:SGTC_530|Compound:4358-1714|FD-Score:3.60|P-value:7.42E-5 ID:SGTC_537|Compound:1334-0398|FD-Score:3.08|P-value:5.86E-4 ID:SGTC_669|Compound:0108-0021|FD-Score:4.80|P-value:2.30E-7 ID:SGTC_716|Compound:1683-5115|FD-Score:3.35|P-value:2.11E-4 ID:SGTC_815|Compound:1582-0027|FD-Score:-5.28|P-value:2.36E-8 ID:SGTC_1131|Compound:3454-3128|FD-Score:3.15|P-value:4.44E-4 ID:SGTC_1245|Compound:0418-0076|FD-Score:3.56|P-value:8.88E-5 ID:SGTC_1263|Compound:0774-1208|FD-Score:2.96|P-value:8.78E-4 ID:SGTC_1286|Compound:0929-0070|FD-Score:3.69|P-value:5.08E-5 ID:SGTC_1480|Compound:1070-0044|FD-Score:-3.44|P-value:1.93E-4 ID:SGTC_1580|Compound:hydrocortisone 21-hemisuccinate|FD-Score:-3.92|P-value:2.62E-5 ID:SGTC_1630|Compound:st005209|FD-Score:5.17|P-value:2.81E-8 ID:SGTC_1780|Compound:st038448|FD-Score:3.72|P-value:4.48E-5 ID:SGTC_1781|Compound:st038725|FD-Score:-4.34|P-value:3.70E-6 ID:SGTC_1848|Compound:st056003|FD-Score:-4.50|P-value:1.67E-6 ID:SGTC_1851|Compound:st056236|FD-Score:3.90|P-value:2.00E-5 ID:SGTC_1857|Compound:st056007|FD-Score:-4.18|P-value:7.98E-6 ID:SGTC_1910|Compound:st062352|FD-Score:-3.12|P-value:6.50E-4 ID:SGTC_1992|Compound:st073643|FD-Score:-3.43|P-value:2.06E-4 ID:SGTC_2175|Compound:5807670|FD-Score:3.01|P-value:7.39E-4 ID:SGTC_2220|Compound:6782180|FD-Score:3.27|P-value:2.82E-4 ID:SGTC_2329|Compound:6138490|FD-Score:2.96|P-value:8.95E-4 ID:SGTC_2407|Compound:paclitaxel|FD-Score:4.11|P-value:7.79E-6 ID:SGTC_2616|Compound:abietic acid|FD-Score:4.41|P-value:1.76E-6 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:-3.85|P-value:3.50E-5 ID:SGTC_2787|Compound:5284540|FD-Score:-3.45|P-value:1.85E-4 ID:SGTC_2901|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:-3.55|P-value:1.25E-4 ID:SGTC_2903|Compound:5923464|FD-Score:-4.21|P-value:6.85E-6 ID:SGTC_2928|Compound:9011484|FD-Score:5.22|P-value:2.16E-8 ID:SGTC_2967|Compound:9089538|FD-Score:3.25|P-value:3.00E-4 ID:SGTC_3326|Compound:9140064|FD-Score:-3.68|P-value:7.27E-5

Top fitness defect scores for YOR303W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_54 0159-0055 7.00 9.0 µM 1.13E-13 ERG2 17 1.31E-12
2 SGTC_2928 9011484 5.22 9.8 µM 2.16E-8 heme biosynthesis & mitochondrial translocase 14 9.10E-8
3 SGTC_1630 st005209 5.17 31.3 µM 2.81E-8 8 1.16E-7
4 SGTC_669 0108-0021 4.80 26.2 µM 2.30E-7 fatty acid desaturase (OLE1) 45 7.96E-7
5 SGTC_2616 abietic acid 4.41 100.0 µM 1.76E-6 23 5.12E-6
6 SGTC_2407 paclitaxel 4.11 400.0 µM 7.79E-6 31 2.00E-5
7 SGTC_1851 st056236 3.90 18.9 µM 2.00E-5 15 4.76E-5
8 SGTC_1780 st038448 3.72 24.5 µM 4.48E-5 iron homeostasis 26 9.94E-5
9 SGTC_1286 0929-0070 3.69 131.0 µM 5.08E-5 20 1.12E-4
10 SGTC_530 4358-1714 3.60 28.0 µM 7.42E-5 56 1.58E-4
11 SGTC_1245 0418-0076 3.56 17.2 µM 8.88E-5 ERG2 45 1.86E-4
12 SGTC_716 1683-5115 3.35 658.0 µM 2.11E-4 44 4.11E-4
13 SGTC_19 0279-0214 3.29 38.4 µM 2.63E-4 TRP & mitochondrial translation 80 5.03E-4
14 SGTC_2220 6782180 3.27 200.0 µM 2.82E-4 RNA processing & uracil transport 16 5.36E-4
15 SGTC_2967 9089538 3.25 49.9 µM 3.00E-4 61 5.68E-4
16 SGTC_299 1273-0059 3.24 1.4 µM 3.19E-4 43 6.01E-4
17 SGTC_1131 3454-3128 3.15 32.3 µM 4.44E-4 86 8.12E-4
18 SGTC_537 1334-0398 3.08 209.0 µM 5.86E-4 63 0.00105
19 SGTC_2175 5807670 3.01 165.3 µM 7.39E-4 59 0.00130
20 SGTC_1263 0774-1208 2.96 64.8 µM 8.78E-4 119 0.00152

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.180 8.56E-26 YOL140W ARG8 hom Acetylornithine aminotransferase, catalyzes the fourth step in the biosynthesis of the arginine precursor ornithine
0.141 2.00E-16 YOL058W ARG1 hom Arginosuccinate synthetase, catalyzes the formation of L-argininosuccinate from citrulline and L-aspartate in the arginine biosynthesis pathway; potential Cdc28p substrate
0.131 2.11E-14 YHR018C ARG4 hom Argininosuccinate lyase, catalyzes the final step in the arginine biosynthesis pathway
0.120 2.72E-12 YDL133C-A RPL41B hom Ribosomal 60S subunit protein L41B; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41B has a paralog, RPL41A, that arose from the whole genome duplication
0.119 5.56E-12 YJR041C URB2 het Nucleolar protein required for normal metabolism of the rRNA primary transcript, proposed to be involved in ribosome biogenesis
0.117 1.23E-11 YOR130C ORT1 hom Ornithine transporter of the mitochondrial inner membrane, exports ornithine from mitochondria as part of arginine biosynthesis; human ortholog is associated with hyperammonaemia-hyperornithinaemia-homocitrullinuria (HHH) syndrome
0.114 3.10E-11 YER069W ARG5,6 hom Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine
0.113 5.87E-11 YJL088W ARG3 hom Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine
0.109 2.06E-10 YJL071W ARG2 hom Acetylglutamate synthase (glutamate N-acetyltransferase), mitochondrial enzyme that catalyzes the first step in the biosynthesis of the arginine precursor ornithine; forms a complex with Arg5,6p
0.106 6.59E-10 YKR049C FMP46 hom Putative redox protein containing a thioredoxin fold; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.101 4.85E-9 YBR299W MAL32 hom Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose
0.094 4.51E-8 YML088W UFO1 hom F-box receptor protein, subunit of the Skp1-Cdc53-F-box receptor (SCF) E3 ubiquitin ligase complex; binds to phosphorylated Ho endonuclease, allowing its ubiquitylation by SCF and subsequent degradation
0.090 1.51E-7 YKR023W_p YKR023W_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.089 2.25E-7 YDR255C RMD5 hom Component of GID Complex that confers ubiquitin ligase (U3) activity; necessary for polyubiquitination and degradation of the gluconeogenic enzyme fructose-1,6-bisphosphatase; forms dimer with Fyv10p that is then recruited to GID Complex by Gid8p; also required for sporulation; conserved protein that has a degenerate RING finger domain
0.089 2.39E-7 YDR097C MSH6 hom Protein required for mismatch repair in mitosis and meiosis, forms a complex with Msh2p to repair both single-base & insertion-deletion mispairs; also involved in interstrand cross-link repair; potentially phosphorylated by Cdc28p