YOR304W / ISW2

ATP-dependent DNA translocase involved in chromatin remodeling; ATPase component that, with Itc1p, forms a complex required for repression of a-specific genes, INO1, and early meiotic genes during mitotic growth

Zygosity: Homozygous strain
fixedexpanded
Profile for YOR304W / ISW2

Click on Significant Values for Screen Details ID:SGTC_64|Compound:000l-0018|FD-Score:4.07|P-value:2.34E-5 ID:SGTC_67|Compound:0335-0847|FD-Score:3.92|P-value:4.45E-5 ID:SGTC_73|Compound:0327-0325|FD-Score:3.34|P-value:4.10E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:4.34|P-value:7.19E-6 ID:SGTC_141|Compound:0443-0274|FD-Score:-6.80|P-value:1.22E-11 ID:SGTC_244|Compound:nsc-207895|FD-Score:-3.18|P-value:9.81E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-5.31|P-value:9.83E-8 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.24|P-value:8.12E-4 ID:SGTC_384|Compound:k007-0144|FD-Score:4.28|P-value:9.43E-6 ID:SGTC_409|Compound:arachidonic acid|FD-Score:3.15|P-value:7.79E-4 ID:SGTC_444|Compound:curcumin|FD-Score:3.87|P-value:5.41E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-4.28|P-value:1.45E-5 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-3.96|P-value:5.38E-5 ID:SGTC_470|Compound:aa-861|FD-Score:-3.31|P-value:6.21E-4 ID:SGTC_658|Compound:3253-0951|FD-Score:3.54|P-value:1.95E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:3.56|P-value:1.81E-4 ID:SGTC_1036|Compound:k216-0215|FD-Score:-3.27|P-value:7.27E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.69|P-value:1.58E-4 ID:SGTC_1297|Compound:1068-0111|FD-Score:-3.18|P-value:9.86E-4 ID:SGTC_1343|Compound:1487-0095|FD-Score:3.47|P-value:2.53E-4 ID:SGTC_1811|Compound:st050069|FD-Score:3.46|P-value:2.62E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-6.53|P-value:7.30E-11 ID:SGTC_2059|Compound:5238311|FD-Score:-3.23|P-value:8.30E-4 ID:SGTC_2176|Compound:5809774|FD-Score:3.83|P-value:6.28E-5 ID:SGTC_2286|Compound:7945697|FD-Score:6.02|P-value:9.86E-10 ID:SGTC_2582|Compound:agaric acid|FD-Score:-5.17|P-value:2.00E-7 ID:SGTC_2587|Compound:piperic acid|FD-Score:-3.25|P-value:7.65E-4 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:3.77|P-value:7.88E-5 ID:SGTC_2602|Compound:kawain|FD-Score:3.45|P-value:2.75E-4 ID:SGTC_2805|Compound:7950783|FD-Score:3.10|P-value:9.35E-4 ID:SGTC_3249|Compound:9135183|FD-Score:3.71|P-value:1.03E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:4.07|P-value:2.34E-5 ID:SGTC_67|Compound:0335-0847|FD-Score:3.92|P-value:4.45E-5 ID:SGTC_73|Compound:0327-0325|FD-Score:3.34|P-value:4.10E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:4.34|P-value:7.19E-6 ID:SGTC_141|Compound:0443-0274|FD-Score:-6.80|P-value:1.22E-11 ID:SGTC_244|Compound:nsc-207895|FD-Score:-3.18|P-value:9.81E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-5.31|P-value:9.83E-8 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.24|P-value:8.12E-4 ID:SGTC_384|Compound:k007-0144|FD-Score:4.28|P-value:9.43E-6 ID:SGTC_409|Compound:arachidonic acid|FD-Score:3.15|P-value:7.79E-4 ID:SGTC_444|Compound:curcumin|FD-Score:3.87|P-value:5.41E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-4.28|P-value:1.45E-5 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-3.96|P-value:5.38E-5 ID:SGTC_470|Compound:aa-861|FD-Score:-3.31|P-value:6.21E-4 ID:SGTC_658|Compound:3253-0951|FD-Score:3.54|P-value:1.95E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:3.56|P-value:1.81E-4 ID:SGTC_1036|Compound:k216-0215|FD-Score:-3.27|P-value:7.27E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.69|P-value:1.58E-4 ID:SGTC_1297|Compound:1068-0111|FD-Score:-3.18|P-value:9.86E-4 ID:SGTC_1343|Compound:1487-0095|FD-Score:3.47|P-value:2.53E-4 ID:SGTC_1811|Compound:st050069|FD-Score:3.46|P-value:2.62E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-6.53|P-value:7.30E-11 ID:SGTC_2059|Compound:5238311|FD-Score:-3.23|P-value:8.30E-4 ID:SGTC_2176|Compound:5809774|FD-Score:3.83|P-value:6.28E-5 ID:SGTC_2286|Compound:7945697|FD-Score:6.02|P-value:9.86E-10 ID:SGTC_2582|Compound:agaric acid|FD-Score:-5.17|P-value:2.00E-7 ID:SGTC_2587|Compound:piperic acid|FD-Score:-3.25|P-value:7.65E-4 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:3.77|P-value:7.88E-5 ID:SGTC_2602|Compound:kawain|FD-Score:3.45|P-value:2.75E-4 ID:SGTC_2805|Compound:7950783|FD-Score:3.10|P-value:9.35E-4 ID:SGTC_3249|Compound:9135183|FD-Score:3.71|P-value:1.03E-4

Top fitness defect scores for YOR304W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2286 7945697 6.02 125.0 µM 9.86E-10 14 8.71E-10
2 SGTC_103 0844-0104 4.34 51.8 µM 7.19E-6 ERAD & cell cycle 126 7.15E-6
3 SGTC_384 k007-0144 4.28 5.2 µM 9.43E-6 ERAD & cell cycle 50 9.41E-6
4 SGTC_64 000l-0018 4.07 272.1 µM 2.34E-5 ubiquinone biosynthesis & proteasome 143 2.35E-5
5 SGTC_67 0335-0847 3.92 266.0 µM 4.45E-5 ubiquinone biosynthesis & proteasome 120 4.52E-5
6 SGTC_444 curcumin 3.87 90.4 µM 5.41E-5 iron homeostasis 28 5.50E-5
7 SGTC_2176 5809774 3.83 34.0 µM 6.28E-5 mitochondrial processes 13 6.40E-5
8 SGTC_2595 hexamethylquercetagetin 3.77 100.0 µM 7.88E-5 30 8.05E-5
9 SGTC_3249 9135183 3.71 49.5 µM 1.03E-4 60S ribosome export 17 1.05E-4
10 SGTC_690 glyoxal 3.56 20.3 µM 1.81E-4 100 1.86E-4
11 SGTC_658 3253-0951 3.54 102.0 µM 1.95E-4 calcium & mitochondrial duress 78 2.01E-4
12 SGTC_1343 1487-0095 3.47 224.0 µM 2.53E-4 15 2.61E-4
13 SGTC_1811 st050069 3.46 61.5 µM 2.62E-4 11 2.71E-4
14 SGTC_2602 kawain 3.45 100.0 µM 2.75E-4 43 2.84E-4
15 SGTC_73 0327-0325 3.34 16.2 µM 4.10E-4 DNA damage response 74 4.25E-4
16 SGTC_409 arachidonic acid 3.15 100.0 µM 7.79E-4 35 8.12E-4
17 SGTC_2805 7950783 3.10 58.4 µM 9.35E-4 65 9.76E-4
18 SGTC_1308 1187-1617 2.96 41.4 µM 0.00149 fatty acid desaturase (OLE1) 101 0.00156
19 SGTC_846 0335-0850 2.90 148.0 µM 0.00177 ubiquinone biosynthesis & proteasome 73 0.00186
20 SGTC_263 alizarin 2.89 107.9 µM 0.00183 iron homeostasis 138 0.00192

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.205 4.24E-33 YMR298W LIP1 het Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis
0.198 3.91E-31 YML014W TRM9 hom tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses
0.196 1.54E-30 YDL148C NOP14 het Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA
0.188 3.54E-28 YGR159C NSR1 hom Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis
0.188 4.72E-28 YGL133W ITC1 hom Subunit of the ATP-dependent Isw2p-Itc1p chromatin remodeling complex, required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1; similar to mammalian Acf1p, the regulatory subunit of the mammalian ATP-utilizing chromatin assembly and modifying factor (ACF) complex
0.180 9.99E-26 YLR021W IRC25 hom Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions
0.179 1.21E-25 YMR022W UBC7 hom Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly
0.176 1.08E-24 YDL060W TSR1 het Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.173 5.25E-24 YLR117C CLF1 het Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins
0.172 9.39E-24 YER019C-A SBH2 hom Ssh1p-Sss1p-Sbh2p complex component, involved in protein translocation into the endoplasmic reticulum; homologous to Sbh1p
0.170 3.55E-23 YDL007W RPT2 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle
0.169 6.51E-23 YOR159C SME1 het Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E
0.164 1.07E-21 YER159C BUR6 het Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha
0.163 1.94E-21 YER041W YEN1 hom Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p
0.154 2.82E-19 YMR067C UBX4 hom UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress