YPL004C / LSP1

Primary component of eisosomes, which are large immobile patch structures at the cell cortex associated with endocytosis, along with Pil1p and Sur7p; null mutants show activation of Pkc1p/Ypk1p stress resistance pathways; member of the BAR domain family

Zygosity: Homozygous strain
fixedexpanded
Profile for YPL004C / LSP1

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:3.59|P-value:8.41E-5 ID:SGTC_179|Compound:1273-0063|FD-Score:-3.71|P-value:5.45E-5 ID:SGTC_374|Compound:0880-0231|FD-Score:3.29|P-value:2.75E-4 ID:SGTC_517|Compound:1365-0151|FD-Score:-5.97|P-value:2.25E-10 ID:SGTC_762|Compound:1165-0429|FD-Score:3.37|P-value:2.01E-4 ID:SGTC_927|Compound:2425-1185|FD-Score:3.65|P-value:6.52E-5 ID:SGTC_933|Compound:2914-0560|FD-Score:3.33|P-value:2.37E-4 ID:SGTC_936|Compound:3005-4620|FD-Score:5.13|P-value:3.79E-8 ID:SGTC_1146|Compound:3579-1023|FD-Score:3.53|P-value:1.06E-4 ID:SGTC_1267|Compound:0828-0272|FD-Score:3.06|P-value:6.57E-4 ID:SGTC_1282|Compound:0886-0010|FD-Score:3.08|P-value:6.11E-4 ID:SGTC_1283|Compound:0922-0125|FD-Score:3.83|P-value:2.93E-5 ID:SGTC_1436|Compound:4092-1007|FD-Score:-3.61|P-value:8.38E-5 ID:SGTC_1628|Compound:st004252|FD-Score:3.11|P-value:5.44E-4 ID:SGTC_2108|Compound:5365811|FD-Score:-3.21|P-value:4.14E-4 ID:SGTC_2331|Compound:7980721|FD-Score:-3.02|P-value:8.24E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.25|P-value:3.30E-4 ID:SGTC_2549|Compound:cedrol|FD-Score:3.01|P-value:8.07E-4 ID:SGTC_2695|Compound:st077686|FD-Score:3.80|P-value:3.39E-5 ID:SGTC_3186|Compound:9110464|FD-Score:-3.10|P-value:6.20E-4 ID:SGTC_3305|Compound:9127027|FD-Score:-3.58|P-value:9.53E-5 ID:SGTC_6|Compound:0844-0013|FD-Score:3.59|P-value:8.41E-5 ID:SGTC_179|Compound:1273-0063|FD-Score:-3.71|P-value:5.45E-5 ID:SGTC_374|Compound:0880-0231|FD-Score:3.29|P-value:2.75E-4 ID:SGTC_517|Compound:1365-0151|FD-Score:-5.97|P-value:2.25E-10 ID:SGTC_762|Compound:1165-0429|FD-Score:3.37|P-value:2.01E-4 ID:SGTC_927|Compound:2425-1185|FD-Score:3.65|P-value:6.52E-5 ID:SGTC_933|Compound:2914-0560|FD-Score:3.33|P-value:2.37E-4 ID:SGTC_936|Compound:3005-4620|FD-Score:5.13|P-value:3.79E-8 ID:SGTC_1146|Compound:3579-1023|FD-Score:3.53|P-value:1.06E-4 ID:SGTC_1267|Compound:0828-0272|FD-Score:3.06|P-value:6.57E-4 ID:SGTC_1282|Compound:0886-0010|FD-Score:3.08|P-value:6.11E-4 ID:SGTC_1283|Compound:0922-0125|FD-Score:3.83|P-value:2.93E-5 ID:SGTC_1436|Compound:4092-1007|FD-Score:-3.61|P-value:8.38E-5 ID:SGTC_1628|Compound:st004252|FD-Score:3.11|P-value:5.44E-4 ID:SGTC_2108|Compound:5365811|FD-Score:-3.21|P-value:4.14E-4 ID:SGTC_2331|Compound:7980721|FD-Score:-3.02|P-value:8.24E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.25|P-value:3.30E-4 ID:SGTC_2549|Compound:cedrol|FD-Score:3.01|P-value:8.07E-4 ID:SGTC_2695|Compound:st077686|FD-Score:3.80|P-value:3.39E-5 ID:SGTC_3186|Compound:9110464|FD-Score:-3.10|P-value:6.20E-4 ID:SGTC_3305|Compound:9127027|FD-Score:-3.58|P-value:9.53E-5

Top fitness defect scores for YPL004C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_936 3005-4620 5.13 401.0 µM 3.79E-8 8 1.44E-7
2 SGTC_1283 0922-0125 3.83 16.4 µM 2.93E-5 24 6.36E-5
3 SGTC_2695 st077686 3.80 52.0 µM 3.39E-5 41 7.25E-5
4 SGTC_927 2425-1185 3.65 123.0 µM 6.52E-5 37 1.32E-4
5 SGTC_6 0844-0013 3.59 19.8 µM 8.41E-5 127 1.67E-4
6 SGTC_1146 3579-1023 3.53 7.0 µM 1.06E-4 27 2.06E-4
7 SGTC_762 1165-0429 3.37 115.0 µM 2.01E-4 TSC3-RPN4 38 3.72E-4
8 SGTC_933 2914-0560 3.33 114.0 µM 2.37E-4 46 4.31E-4
9 SGTC_374 0880-0231 3.29 82.3 µM 2.75E-4 iron homeostasis 52 4.95E-4
10 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 3.25 3.7 µM 3.30E-4 158 5.85E-4
11 SGTC_1628 st004252 3.11 77.7 µM 5.44E-4 61 9.24E-4
12 SGTC_1282 0886-0010 3.08 32.4 µM 6.11E-4 endomembrane recycling 66 0.00103
13 SGTC_1267 0828-0272 3.06 9.5 µM 6.57E-4 53 0.00110
14 SGTC_2549 cedrol 3.01 20.9 µM 8.07E-4 45 0.00133
15 SGTC_3298 9116858 2.94 71.4 µM 0.00101 48 0.00163
16 SGTC_1110 3-hydroxy-3'-methoxyflavone 2.91 1.2 µM 0.00114 iron homeostasis 105 0.00182
17 SGTC_3120 9124077 2.88 49.5 µM 0.00126 53 0.00200
18 SGTC_1108 betulinic acid 2.85 729.1 nM 0.00138 63 0.00218
19 SGTC_1145 3546-0597 2.80 10.2 µM 0.00166 mitochondrial processes 86 0.00257
20 SGTC_3296 9122258 2.76 64.0 µM 0.00186 redox potentiating 76 0.00285

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.193 1.84E-29 YMR080C NAM7 hom ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress
0.189 2.46E-28 YDR374C_p YDR374C_p hom Putative protein of unknown function
0.185 3.21E-27 YNL218W MGS1 hom Protein with DNA-dependent ATPase and ssDNA annealing activities; involved in maintenance of genome; interacts functionally with DNA polymerase delta; homolog of human Werner helicase interacting protein (WHIP); forms nuclear foci upon DNA replication stress
0.180 9.37E-26 YDR085C AFR1 hom Protein required for pheromone-induced projection (shmoo) formation; regulates septin architecture during mating; has an RVXF motif that mediates targeting of Glc7p to mating projections; interacts with Cdc12p; AFR1 has a paralog, YER158C, that arose from the whole genome duplication
0.166 3.22E-22 YGR226C_d YGR226C_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; overlaps significantly with a verified ORF, AMA1/YGR225W
0.165 8.05E-22 YDL049C KNH1 hom Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance
0.164 1.20E-21 YHR021W-A_p ECM12_p hom Putative protein of unknown function; may contribute to cell wall biosynthesis, mutants display zymolyase hypersensitivity
0.158 3.52E-20 YER118C SHO1 hom Transmembrane osmosensor involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p
0.157 5.86E-20 YOR214C_p YOR214C_p hom Putative protein of unknown function; YOR214C is not an essential gene
0.154 2.47E-19 YKL149C DBR1 hom RNA lariat debranching enzyme, involved in intron turnover; required for efficient Ty1 transposition
0.148 7.77E-18 YHL002W HSE1 hom Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes
0.142 1.50E-16 YFL003C MSH4 hom Protein involved in meiotic recombination, required for normal levels of crossing over, colocalizes with Zip2p to discrete foci on meiotic chromosomes, has homology to bacterial MutS protein
0.139 5.51E-16 YBL019W APN2 hom Class II abasic (AP) endonuclease involved in repair of DNA damage; homolog of human HAP1 and E. coli exoIII
0.134 6.86E-15 YOR107W RGS2 hom Negative regulator of glucose-induced cAMP signaling; directly activates the GTPase activity of the heterotrimeric G protein alpha subunit Gpa2p
0.130 4.36E-14 YOR155C ISN1 hom Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase, catalyzes the breakdown of IMP to inosine, does not show similarity to known 5'-nucleotidases from other organisms