HIPHOP chemogenomics database

YPL043W / NOP4

Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs)

Zygosity: Heterozygous strain

fixedexpanded
Profile for YPL043W / NOP4

Click on Significant Values for Screen Details

Top fitness defect scores for YPL043W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_423	5-fluorouracil	12.20	457.0 µM	1.06E-41	exosome	3	1.05E-34	2.32
2	SGTC_610	4215-0184	9.02	155.0 µM	1.52E-23	exosome	4	9.73E-20	0.22
3	SGTC_1698	5-fluorocytosine	6.12	1.1 µM	8.16E-12	exosome	8	4.72E-10	0.11
4	SGTC_242	5hr heat shock (37°C) + 5-fluorouracil	5.72	4.6 µM	1.55E-10	heat shock/prefoldin	10	5.39E-9	0.04
5	SGTC_1074	carmofur	5.36	3.8 µM	1.79E-9	exosome	17	4.09E-8	0.02
6	SGTC_2689	tacrolimus	5.34	100.0 µM	2.02E-9		5	4.52E-8	0.43
7	SGTC_44	0929-0055	5.28	61.2 µM	3.04E-9		3	6.34E-8	0.90
8	SGTC_526	4469-0568	5.05	170.0 µM	1.41E-8	azole & statin	5	2.26E-7	1.01
9	SGTC_1077	5-fluorocytosine	5.00	377.0 nM	1.92E-8	exosome	14	2.92E-7	0.61
10	SGTC_3330	9141424	4.83	71.4 µM	5.22E-8	amide catabolism	1	6.69E-7	0.71
11	SGTC_431	k089-0097	4.58	107.0 µM	2.38E-7	iron homeostasis	8	2.36E-6	0.03
12	SGTC_616	4092-0450	4.33	108.0 µM	9.60E-7		3	7.51E-6	0.63
13	SGTC_1953	st076800	4.14	12.1 µM	2.67E-6		15	1.76E-5	0.39
14	SGTC_2687	chenodiol	3.99	100.0 µM	5.60E-6		4	3.25E-5	0.03
15	SGTC_42	0133-0086	3.84	60.6 µM	1.23E-5		9	6.26E-5	0.01
16	SGTC_99	0109-0120	3.77	622.8 µM	1.72E-5		8	8.27E-5	0.14
17	SGTC_118	0083-0097	3.74	135.3 µM	1.95E-5	translation	24	9.20E-5	0.00
18	SGTC_3351	9150312	3.68	21.6 µM	2.59E-5	cell wall	10	1.16E-4	0.02
19	SGTC_538	2922-0838	3.68	53.3 µM	2.66E-5	Golgi	7	1.19E-4	0.28
20	SGTC_38	0929-0051	3.64	58.3 µM	3.18E-5		6	1.38E-4	0.07

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.464	9.46E-179	YGR195W	SKI6	het	Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4)
0.388	2.72E-121	YDL111C	RRP42	het	Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7)
0.388	4.11E-121	YPL044C_d	YPL044C_d	het	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W
0.387	5.60E-120	YKL172W	EBP2	het	Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering
0.341	9.12E-92	YDR280W	RRP45	het	Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress
0.334	2.78E-88	YGR158C	MTR3	het	Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6)
0.331	9.68E-87	YOL021C	DIS3	het	Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress
0.327	2.86E-84	YPR142C_d	YPR142C_d	het	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing
0.285	9.96E-64	YPR143W	RRP15	het	Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs
0.254	1.48E-50	YGR095C	RRP46	het	Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5)
0.210	6.37E-35	YKL021C	MAK11	het	Protein involved in an early, nucleolar step of 60S ribosomal subunit biogenesis; essential for cell growth and replication of killer M1 dsRNA virus; contains four beta-transducin repeats
0.203	1.38E-32	YNL061W	NOP2	het	Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles
0.196	2.46E-30	YNL002C	RLP7	het	Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs
0.188	5.23E-28	YDR060W	MAK21	het	Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein
0.179	1.48E-25	YNL232W	CSL4	het	Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; has similarity to human hCsl4p (EXOSC1)