YPL187W / MF(ALPHA)1

Mating pheromone alpha-factor, made by alpha cells; interacts with mating type a cells to induce cell cycle arrest and other responses leading to mating; also encoded by MF(ALPHA)2, although MF(ALPHA)1 produces most alpha-factor

Zygosity: Homozygous strain
fixedexpanded
Profile for YPL187W / MF(ALPHA)1

Click on Significant Values for Screen Details ID:SGTC_155|Compound:k072-0202|FD-Score:-3.79|P-value:7.71E-5 ID:SGTC_183|Compound:3914-0051|FD-Score:3.80|P-value:7.95E-5 ID:SGTC_530|Compound:4358-1714|FD-Score:3.20|P-value:7.20E-4 ID:SGTC_803|Compound:4488-1585|FD-Score:3.36|P-value:4.21E-4 ID:SGTC_979|Compound:1426-2828|FD-Score:3.68|P-value:1.23E-4 ID:SGTC_1134|Compound:3477-0009|FD-Score:-3.32|P-value:4.63E-4 ID:SGTC_1184|Compound:1269-1718|FD-Score:-4.04|P-value:2.75E-5 ID:SGTC_1254|Compound:0550-0035|FD-Score:3.79|P-value:8.24E-5 ID:SGTC_1509|Compound:1326-1131|FD-Score:-3.15|P-value:8.10E-4 ID:SGTC_1571|Compound:st070080|FD-Score:3.33|P-value:4.70E-4 ID:SGTC_1584|Compound:6-fluoro-dl-tryptophan|FD-Score:3.47|P-value:2.80E-4 ID:SGTC_1759|Compound:st043059|FD-Score:3.25|P-value:6.20E-4 ID:SGTC_1971|Compound:st069429|FD-Score:3.18|P-value:7.86E-4 ID:SGTC_2057|Compound:5236571|FD-Score:4.34|P-value:7.99E-6 ID:SGTC_2222|Compound:6885208|FD-Score:-3.26|P-value:5.62E-4 ID:SGTC_2476|Compound:5806916|FD-Score:4.37|P-value:6.80E-6 ID:SGTC_2481|Compound:5478948|FD-Score:4.21|P-value:1.41E-5 ID:SGTC_2616|Compound:abietic acid|FD-Score:3.87|P-value:5.91E-5 ID:SGTC_2736|Compound:butenafine|FD-Score:3.23|P-value:6.49E-4 ID:SGTC_2748|Compound:diazoxide|FD-Score:4.10|P-value:2.23E-5 ID:SGTC_2810|Compound:7959871|FD-Score:-5.66|P-value:8.09E-9 ID:SGTC_2813|Compound:7971799|FD-Score:-4.30|P-value:8.74E-6 ID:SGTC_2831|Compound:7999036|FD-Score:3.19|P-value:7.63E-4 ID:SGTC_2898|Compound:9056270|FD-Score:3.86|P-value:6.20E-5 ID:SGTC_2921|Compound:7974240|FD-Score:3.50|P-value:2.52E-4 ID:SGTC_2972|Compound:9093619|FD-Score:4.20|P-value:1.48E-5 ID:SGTC_155|Compound:k072-0202|FD-Score:-3.79|P-value:7.71E-5 ID:SGTC_183|Compound:3914-0051|FD-Score:3.80|P-value:7.95E-5 ID:SGTC_530|Compound:4358-1714|FD-Score:3.20|P-value:7.20E-4 ID:SGTC_803|Compound:4488-1585|FD-Score:3.36|P-value:4.21E-4 ID:SGTC_979|Compound:1426-2828|FD-Score:3.68|P-value:1.23E-4 ID:SGTC_1134|Compound:3477-0009|FD-Score:-3.32|P-value:4.63E-4 ID:SGTC_1184|Compound:1269-1718|FD-Score:-4.04|P-value:2.75E-5 ID:SGTC_1254|Compound:0550-0035|FD-Score:3.79|P-value:8.24E-5 ID:SGTC_1509|Compound:1326-1131|FD-Score:-3.15|P-value:8.10E-4 ID:SGTC_1571|Compound:st070080|FD-Score:3.33|P-value:4.70E-4 ID:SGTC_1584|Compound:6-fluoro-dl-tryptophan|FD-Score:3.47|P-value:2.80E-4 ID:SGTC_1759|Compound:st043059|FD-Score:3.25|P-value:6.20E-4 ID:SGTC_1971|Compound:st069429|FD-Score:3.18|P-value:7.86E-4 ID:SGTC_2057|Compound:5236571|FD-Score:4.34|P-value:7.99E-6 ID:SGTC_2222|Compound:6885208|FD-Score:-3.26|P-value:5.62E-4 ID:SGTC_2476|Compound:5806916|FD-Score:4.37|P-value:6.80E-6 ID:SGTC_2481|Compound:5478948|FD-Score:4.21|P-value:1.41E-5 ID:SGTC_2616|Compound:abietic acid|FD-Score:3.87|P-value:5.91E-5 ID:SGTC_2736|Compound:butenafine|FD-Score:3.23|P-value:6.49E-4 ID:SGTC_2748|Compound:diazoxide|FD-Score:4.10|P-value:2.23E-5 ID:SGTC_2810|Compound:7959871|FD-Score:-5.66|P-value:8.09E-9 ID:SGTC_2813|Compound:7971799|FD-Score:-4.30|P-value:8.74E-6 ID:SGTC_2831|Compound:7999036|FD-Score:3.19|P-value:7.63E-4 ID:SGTC_2898|Compound:9056270|FD-Score:3.86|P-value:6.20E-5 ID:SGTC_2921|Compound:7974240|FD-Score:3.50|P-value:2.52E-4 ID:SGTC_2972|Compound:9093619|FD-Score:4.20|P-value:1.48E-5

Top fitness defect scores for YPL187W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2476 5806916 4.37 10.5 µM 6.80E-6 9 6.17E-6
2 SGTC_2057 5236571 4.34 38.3 µM 7.99E-6 RPP1 & pyrimidine depletion 20 7.26E-6
3 SGTC_2481 5478948 4.21 45.2 µM 1.41E-5 PDR1 29 1.29E-5
4 SGTC_2972 9093619 4.20 59.9 µM 1.48E-5 17 1.35E-5
5 SGTC_2748 diazoxide 4.10 86.7 µM 2.23E-5 7 2.05E-5
6 SGTC_2616 abietic acid 3.87 100.0 µM 5.91E-5 32 5.45E-5
7 SGTC_2898 9056270 3.86 58.4 µM 6.20E-5 7 5.72E-5
8 SGTC_183 3914-0051 3.80 30.6 µM 7.95E-5 60S ribosome export 27 7.35E-5
9 SGTC_1254 0550-0035 3.79 264.0 µM 8.24E-5 23 7.63E-5
10 SGTC_979 1426-2828 3.68 121.0 µM 1.23E-4 23 1.14E-4
11 SGTC_2921 7974240 3.50 10.7 µM 2.52E-4 73 2.35E-4
12 SGTC_1584 6-fluoro-dl-tryptophan 3.47 90.0 µM 2.80E-4 mitochondrial processes 28 2.62E-4
13 SGTC_803 4488-1585 3.36 147.0 µM 4.21E-4 28 3.95E-4
14 SGTC_1571 st070080 3.33 73.4 µM 4.70E-4 36 4.41E-4
15 SGTC_1759 st043059 3.25 70.0 µM 6.20E-4 amide catabolism 53 5.83E-4
16 SGTC_2736 butenafine 3.23 14.7 µM 6.49E-4 54 6.10E-4
17 SGTC_530 4358-1714 3.20 28.0 µM 7.20E-4 76 6.78E-4
18 SGTC_2831 7999036 3.19 19.5 µM 7.63E-4 87 7.19E-4
19 SGTC_1971 st069429 3.18 63.5 µM 7.86E-4 60S ribosome export 36 7.40E-4
20 SGTC_1108 betulinic acid 3.10 729.1 nM 0.00101 41 9.53E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.115 2.37E-11 YLR168C UPS2 hom Mitochondrial protein involved in phospholipid metabolism; localizes to the intermembrane space; has a role in regulation of phospholipid metabolism by inhibiting conversion of phosphatidylethanolamine to phosphatidylcholine; null mutant has defects in mitochondrial morphology; similar to Ups1p, Ups3p and to human PRELI
0.108 4.10E-10 YMR182W-A_p YMR182W-A_p hom Putative protein of unknown function
0.098 1.51E-8 YGL085W_p LCL3_p hom Putative protein of unknown function; mutant has long chronological lifespan; has homology to Staphylococcus aureus nuclease; GFP-fusion protein localizes to mitochondria; is induced in response to the DNA-damaging agent MMS
0.087 4.98E-7 YKL096W CWP1 hom Cell wall mannoprotein that localizes specifically to birth scars of daughter cells, linked to a beta-1,3- and beta-1,6-glucan heteropolymer through a phosphodiester bond; required for propionic acid resistance
0.086 7.03E-7 YBR207W FTH1 hom Putative high affinity iron transporter; involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis; protein abundance increases in response to DNA replication stress
0.084 1.05E-6 YBR257W POP4 het Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
0.083 1.28E-6 YBR052C RFS1 hom Protein of unknown function; member of a flavodoxin-like fold protein family that includes Pst2p and Ycp4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; RFS1 has a paralog, PST2, that arose from the whole genome duplication
0.083 1.40E-6 YMR213W CEF1 het Essential splicing factor; associated with Prp19p and the spliceosome, contains an N-terminal c-Myb DNA binding motif necessary for cell viability but not for Prp19p association, evolutionarily conserved and homologous to S. pombe Cdc5p
0.081 2.30E-6 YNL118C DCP2 het Catalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex; removes the 5' cap structure from mRNAs prior to their degradation; nudix hydrolase family member; forms cytoplasmic foci upon DNA replication stress
0.081 2.54E-6 YIR004W DJP1 hom Cytosolic J-domain-containing protein, required for peroxisomal protein import and involved in peroxisome assembly, homologous to E. coli DnaJ
0.081 2.73E-6 YBR129C OPY1 hom Protein of unknown function, overproduction blocks cell cycle arrest in the presence of mating pheromone; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.080 3.48E-6 YNL251C NRD1 het RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with the C-terminal domain of the RNA polymerase II large subunit (Rpo21p), preferentially at phosphorylated Ser5, to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites
0.080 3.66E-6 YMR105C PGM2 hom Phosphoglucomutase; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism; functions as the acceptor for a Glc-phosphotransferase; protein abundance increases in response to DNA replication stress
0.079 4.28E-6 YJR127C RSF2 hom Zinc-finger protein; involved in transcriptional control of both nuclear and mitochondrial genes, many of which specify products required for glycerol-based growth, respiration, and other functions; RSF2 has a paralog, TDA9, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.079 4.49E-6 YBR097W VPS15 hom Serine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; interacts with the GDP-bound form of Gpa1p; myristoylated; a fraction is localized, with Vps34p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery