Putative substrate of cAMP-dependent protein kinase (PKA); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YPL260W is not an essential gene; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
Profile for YPL260W / YPL260W

Click on Significant Values for Screen Details

Top fitness defect scores for YPL260W deletion by condition

RankScreen IDConditionFD ScoreConc
1 SGTC_1670 st016595 7.06 4.0 µM 4.56E-11 1 8.37E-13
2 SGTC_926 2268-1401 6.17 59.4 µM 7.45E-9 TSC3-RPN4 11 3.40E-10
3 SGTC_947 3447-0010 5.82 211.0 µM 4.57E-8 3 2.88E-9
4 SGTC_1774 st048371 5.63 48.5 µM 1.22E-7 RNA processing & uracil transport 5 9.18E-9
5 SGTC_3211 9128781 5.32 49.5 µM 5.39E-7 29 5.28E-8
6 SGTC_2809 7964133 5.26 13.0 µM 6.90E-7 60S ribosome export 12 7.06E-8
7 SGTC_3008 9078912 5.16 71.4 µM 1.12E-6 2 1.25E-7
8 SGTC_2729 fluphenazine 4.83 10.7 µM 4.70E-6 2 6.75E-7
9 SGTC_123 0083-0120 4.68 66.7 µM 8.75E-6 28 1.40E-6
10 SGTC_1528 arabinosylhypoxanthine 4.64 74.6 µM 1.04E-5 12 1.72E-6
11 SGTC_17 0960-0276 4.51 49.8 µM 1.78E-5 16 3.24E-6
12 SGTC_1920 st075728 4.46 45.7 µM 2.20E-5 10 4.15E-6
13 SGTC_2694 st077685 4.34 81.9 µM 3.50E-5 endomembrane recycling 20 7.17E-6
14 SGTC_927 2425-1185 4.29 123.0 µM 4.14E-5 22 8.74E-6
15 SGTC_1252 0493-0875 4.22 34.8 µM 5.48E-5 23 1.21E-5
16 SGTC_1373 2887-0003 4.12 58.5 µM 8.11E-5 Golgi 19 1.93E-5
17 SGTC_1899 st063254 3.85 59.6 µM 2.12E-4 17 5.96E-5
18 SGTC_1632 st005650 3.73 90.0 µM 3.17E-4 37 9.55E-5
19 SGTC_1053 3366-3204 3.70 134.0 µM 3.52E-4 cell wall 43 1.08E-4
20 SGTC_1614 st002383 3.58 48.6 µM 5.25E-4 cell wall signaling 23 1.73E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.115 2.20E-11 YGR204W ADE3 hom Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine
0.113 4.46E-11 YJR148W BAT2 hom Cytosolic branched-chain amino acid (BCAA) aminotransferase, preferentially involved in BCAA catabolism; homolog of murine ECA39; highly expressed during stationary phase and repressed during logarithmic phase
0.112 6.57E-11 YOR315W SFG1 hom Nuclear protein, putative transcription factor required for growth of superficial pseudohyphae (which do not invade the agar substrate) but not for invasive pseudohyphal growth; may act together with Phd1p; potential Cdc28p substrate
0.111 1.28E-10 YDL123W SNA4 hom Protein of unknown function, localized to the vacuolar outer membrane; predicted to be palmitoylated
0.095 4.29E-8 YOL140W ARG8 hom Acetylornithine aminotransferase, catalyzes the fourth step in the biosynthesis of the arginine precursor ornithine
0.093 6.88E-8 YOR123C LEO1 hom Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay
0.088 2.84E-7 YER069W ARG5,6 hom Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine
0.088 3.85E-7 YML096W_p YML096W_p hom Putative protein of unknown function with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10
0.087 3.94E-7 YOL044W PEX15 hom Phosphorylated tail-anchored type II integral peroxisomal membrane protein required for peroxisome biogenesis, cells lacking Pex15p mislocalize peroxisomal matrix proteins to cytosol, overexpression results in impaired peroxisome assembly
0.083 1.65E-6 YGR122C-A_d YGR122C-A_d hom Dubious open reading frame unlikely to encode a functional protein, similar to YLR334C and YOL106W
0.082 1.92E-6 YKL005C BYE1 hom Negative regulator of transcription elongation, contains a TFIIS-like domain and a PHD finger, multicopy suppressor of temperature-sensitive ess1 mutations, probably binds RNA polymerase II large subunit
0.080 3.47E-6 YOR323C PRO2 hom Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis
0.079 4.21E-6 YGL184C STR3 hom Peroxisomal cystathionine beta-lyase, converts cystathionine into homocysteine; may be redox regulated by Gto1p
0.079 4.26E-6 YJR046W TAH11 het DNA replication licensing factor, required for pre-replication complex assembly
0.079 4.56E-6 YGR024C THG1 het tRNAHis guanylyltransferase, adds a guanosine residue to the 5' end of tRNAHis after transcription and RNase P cleavage; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes