HIPHOP chemogenomics database

YPR010C / RPA135

RNA polymerase I second largest subunit A135

Zygosity: Heterozygous strain

fixedexpanded
Profile for YPR010C / RPA135

Click on Significant Values for Screen Details

Top fitness defect scores for YPR010C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_2130	NSC 7	4.74	2.5 µM	1.41E-7	copper-dependent oxidative stress	3	1.05E-6	0.37
2	SGTC_1059	3448-6867	4.38	171.0 µM	1.07E-6		2	6.00E-6	0.38
3	SGTC_156	3474-0145	3.95	5.6 µM	9.60E-6	endomembrane recycling	5	3.94E-5	0.03
4	SGTC_2616	abietic acid	3.67	100.0 µM	3.54E-5		5	1.21E-4	0.00
5	SGTC_629	3448-6466	3.65	76.7 µM	3.94E-5		4	1.33E-4	0.07
6	SGTC_751	0833-0568	3.56	350.0 µM	5.70E-5		3	1.83E-4	0.16
7	SGTC_404	fumagillin	3.48	50.0 µM	8.07E-5		9	2.47E-4	0.08
8	SGTC_967	1306-0166	3.43	352.0 µM	1.02E-4		1	3.01E-4	0.14
9	SGTC_2103	5378138	3.21	10.0 µM	2.58E-4		6	6.71E-4	0.01
10	SGTC_2620	irigenin	3.08	100.0 µM	4.32E-4		28	0.00105	0.03
11	SGTC_3042	9091578	3.00	49.5 µM	5.78E-4	ERG2	5	0.00135	0.20
12	SGTC_2125	5246583	3.00	200.0 µM	5.84E-4	tubulin folding & SWR complex	16	0.00136	0.05
13	SGTC_1370	2001-0092	2.97	44.6 µM	6.56E-4		13	0.00150	0.05
14	SGTC_987	1497-1013	2.96	404.0 µM	6.62E-4		12	0.00151	0.05
15	SGTC_1691	st019933	2.95	84.6 µM	7.01E-4		8	0.00159	0.07
16	SGTC_1000	4048-0884	2.91	8.7 µM	8.17E-4	copper-dependent oxidative stress	24	0.00182	0.01
17	SGTC_2605	digitonin	2.89	300.0 nM	8.83E-4		14	0.00194	0.01
18	SGTC_2121	5257820	2.88	149.6 µM	8.92E-4		12	0.00196	0.07
19	SGTC_1379	2144-0755	2.84	36.5 µM	0.00105		16	0.00226	0.04
20	SGTC_1262	0737-0114	2.82	49.6 µM	0.00113		15	0.00240	0.03

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.244	8.27E-47	YBR207W	FTH1	hom	Putative high affinity iron transporter; involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis; protein abundance increases in response to DNA replication stress
0.224	3.04E-39	YMR139W	RIM11	hom	Protein kinase; required for signal transduction during entry into meiosis; promotes the formation of the Ime1p-Ume6p complex by phosphorylating Ime1p and Ume6p; shares similarity with mammalian glycogen synthase kinase 3-beta; protein abundance increases in response to DNA replication stress
0.223	3.20E-39	YLR353W	BUD8	hom	Protein involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the proximal pole
0.222	9.37E-39	YDL110C	TMA17	hom	Protein of unknown function that associates with ribosomes; heterozygous deletion demonstrated increases in chromosome instability in a rad9 deletion background; protein abundance is decreased upon intracellular iron depletion
0.222	9.45E-39	YER067C-A_d	YER067C-A_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W
0.221	2.39E-38	YHR003C	YHR003C	hom	Protein of unknown function, localized to the mitochondrial outer membrane
0.221	2.58E-38	YKL131W_d	YKL131W_d	hom	Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.216	9.16E-37	YMR147W_p	YMR147W_p	hom	Putative protein of unknown function
0.216	1.07E-36	YKL122C	SRP21	het	Subunit of the signal recognition particle (SRP), which functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm
0.213	6.53E-36	YKL179C	COY1	hom	Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function
0.213	9.16E-36	YFR027W	ECO1	het	Acetyltransferase; required for establishment of sister chromatid cohesion; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to ds DNA breaks; has a C2H2-type zinc finger; mutations in human homolog ESCO2 cause Roberts syndrome; relative distribution to the nucleus increases upon DNA replication stress
0.211	4.79E-35	YFL063W_d	YFL063W_d	hom	Dubious open reading frame, based on available experimental and comparative sequence data
0.202	3.38E-32	YLR016C	PML1	hom	Subunit of the RES complex, which is required for nuclear retention of unspliced pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p
0.201	5.40E-32	YDL174C	DLD1	hom	D-lactate dehydrogenase, oxidizes D-lactate to pyruvate, transcription is heme-dependent, repressed by glucose, and derepressed in ethanol or lactate; located in the mitochondrial inner membrane
0.200	1.72E-31	YLR205C	HMX1	hom	ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants