YPR145W / ASN1

Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN1 has a paralog, ASN2, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YPR145W / ASN1

Click on Significant Values for Screen Details ID:SGTC_71|Compound:0417-1750|FD-Score:-5.37|P-value:1.33E-7 ID:SGTC_217|Compound:ketoconazole|FD-Score:3.75|P-value:1.73E-4 ID:SGTC_394|Compound:menadione|FD-Score:3.80|P-value:1.45E-4 ID:SGTC_404|Compound:fumagillin|FD-Score:3.47|P-value:4.66E-4 ID:SGTC_574|Compound:1315-0397|FD-Score:-3.44|P-value:4.89E-4 ID:SGTC_602|Compound:3130-1533|FD-Score:3.32|P-value:7.78E-4 ID:SGTC_731|Compound:3966-0296|FD-Score:3.41|P-value:5.80E-4 ID:SGTC_738|Compound:1315-0003|FD-Score:3.72|P-value:1.93E-4 ID:SGTC_918|Compound:glabranin|FD-Score:3.64|P-value:2.63E-4 ID:SGTC_956|Compound:1185-0240|FD-Score:4.43|P-value:1.20E-5 ID:SGTC_1052|Compound:3253-3998|FD-Score:3.90|P-value:9.93E-5 ID:SGTC_1068|Compound:loratadine|FD-Score:4.83|P-value:2.08E-6 ID:SGTC_1070|Compound:indatraline|FD-Score:7.37|P-value:1.03E-12 ID:SGTC_1085|Compound:doxorubicin|FD-Score:5.07|P-value:6.76E-7 ID:SGTC_1215|Compound:3852-0577|FD-Score:5.91|P-value:8.62E-9 ID:SGTC_1224|Compound:0823-0416|FD-Score:3.70|P-value:2.14E-4 ID:SGTC_1256|Compound:0570-0116|FD-Score:4.87|P-value:1.72E-6 ID:SGTC_1716|Compound:st033144|FD-Score:3.46|P-value:4.93E-4 ID:SGTC_1824|Compound:st053723|FD-Score:4.14|P-value:3.90E-5 ID:SGTC_1919|Compound:st067113|FD-Score:3.69|P-value:2.15E-4 ID:SGTC_1989|Compound:st071868|FD-Score:3.78|P-value:1.59E-4 ID:SGTC_1994|Compound:st072947|FD-Score:4.97|P-value:1.08E-6 ID:SGTC_2065|Compound:5221596|FD-Score:-3.72|P-value:1.84E-4 ID:SGTC_2074|Compound:5212007|FD-Score:3.46|P-value:4.87E-4 ID:SGTC_2120|Compound:5538279|FD-Score:4.72|P-value:3.43E-6 ID:SGTC_2273|Compound:7815444|FD-Score:3.31|P-value:7.94E-4 ID:SGTC_2303|Compound:7774051|FD-Score:-3.55|P-value:3.34E-4 ID:SGTC_2364|Compound:9055074|FD-Score:-3.25|P-value:9.33E-4 ID:SGTC_2366|Compound:9070441|FD-Score:-3.76|P-value:1.58E-4 ID:SGTC_2394|Compound:5221648|FD-Score:4.23|P-value:2.80E-5 ID:SGTC_2395|Compound:5646831|FD-Score:-3.25|P-value:9.04E-4 ID:SGTC_2415|Compound:st077333|FD-Score:3.40|P-value:6.00E-4 ID:SGTC_2445|Compound:5742597|FD-Score:4.32|P-value:1.92E-5 ID:SGTC_2591|Compound:skatole|FD-Score:5.24|P-value:2.87E-7 ID:SGTC_2629|Compound:bisabolol|FD-Score:-3.37|P-value:6.23E-4 ID:SGTC_2643|Compound:anethole|FD-Score:3.25|P-value:9.85E-4 ID:SGTC_2884|Compound:9058502|FD-Score:5.04|P-value:7.60E-7 ID:SGTC_2905|Compound:7799065|FD-Score:4.98|P-value:1.02E-6 ID:SGTC_2969|Compound:9091408|FD-Score:4.41|P-value:1.31E-5 ID:SGTC_3020|Compound:9089140|FD-Score:3.28|P-value:8.90E-4 ID:SGTC_3026|Compound:9090633|FD-Score:3.72|P-value:1.93E-4 ID:SGTC_3083|Compound:9117465|FD-Score:3.65|P-value:2.55E-4 ID:SGTC_71|Compound:0417-1750|FD-Score:-5.37|P-value:1.33E-7 ID:SGTC_217|Compound:ketoconazole|FD-Score:3.75|P-value:1.73E-4 ID:SGTC_394|Compound:menadione|FD-Score:3.80|P-value:1.45E-4 ID:SGTC_404|Compound:fumagillin|FD-Score:3.47|P-value:4.66E-4 ID:SGTC_574|Compound:1315-0397|FD-Score:-3.44|P-value:4.89E-4 ID:SGTC_602|Compound:3130-1533|FD-Score:3.32|P-value:7.78E-4 ID:SGTC_731|Compound:3966-0296|FD-Score:3.41|P-value:5.80E-4 ID:SGTC_738|Compound:1315-0003|FD-Score:3.72|P-value:1.93E-4 ID:SGTC_918|Compound:glabranin|FD-Score:3.64|P-value:2.63E-4 ID:SGTC_956|Compound:1185-0240|FD-Score:4.43|P-value:1.20E-5 ID:SGTC_1052|Compound:3253-3998|FD-Score:3.90|P-value:9.93E-5 ID:SGTC_1068|Compound:loratadine|FD-Score:4.83|P-value:2.08E-6 ID:SGTC_1070|Compound:indatraline|FD-Score:7.37|P-value:1.03E-12 ID:SGTC_1085|Compound:doxorubicin|FD-Score:5.07|P-value:6.76E-7 ID:SGTC_1215|Compound:3852-0577|FD-Score:5.91|P-value:8.62E-9 ID:SGTC_1224|Compound:0823-0416|FD-Score:3.70|P-value:2.14E-4 ID:SGTC_1256|Compound:0570-0116|FD-Score:4.87|P-value:1.72E-6 ID:SGTC_1716|Compound:st033144|FD-Score:3.46|P-value:4.93E-4 ID:SGTC_1824|Compound:st053723|FD-Score:4.14|P-value:3.90E-5 ID:SGTC_1919|Compound:st067113|FD-Score:3.69|P-value:2.15E-4 ID:SGTC_1989|Compound:st071868|FD-Score:3.78|P-value:1.59E-4 ID:SGTC_1994|Compound:st072947|FD-Score:4.97|P-value:1.08E-6 ID:SGTC_2065|Compound:5221596|FD-Score:-3.72|P-value:1.84E-4 ID:SGTC_2074|Compound:5212007|FD-Score:3.46|P-value:4.87E-4 ID:SGTC_2120|Compound:5538279|FD-Score:4.72|P-value:3.43E-6 ID:SGTC_2273|Compound:7815444|FD-Score:3.31|P-value:7.94E-4 ID:SGTC_2303|Compound:7774051|FD-Score:-3.55|P-value:3.34E-4 ID:SGTC_2364|Compound:9055074|FD-Score:-3.25|P-value:9.33E-4 ID:SGTC_2366|Compound:9070441|FD-Score:-3.76|P-value:1.58E-4 ID:SGTC_2394|Compound:5221648|FD-Score:4.23|P-value:2.80E-5 ID:SGTC_2395|Compound:5646831|FD-Score:-3.25|P-value:9.04E-4 ID:SGTC_2415|Compound:st077333|FD-Score:3.40|P-value:6.00E-4 ID:SGTC_2445|Compound:5742597|FD-Score:4.32|P-value:1.92E-5 ID:SGTC_2591|Compound:skatole|FD-Score:5.24|P-value:2.87E-7 ID:SGTC_2629|Compound:bisabolol|FD-Score:-3.37|P-value:6.23E-4 ID:SGTC_2643|Compound:anethole|FD-Score:3.25|P-value:9.85E-4 ID:SGTC_2884|Compound:9058502|FD-Score:5.04|P-value:7.60E-7 ID:SGTC_2905|Compound:7799065|FD-Score:4.98|P-value:1.02E-6 ID:SGTC_2969|Compound:9091408|FD-Score:4.41|P-value:1.31E-5 ID:SGTC_3020|Compound:9089140|FD-Score:3.28|P-value:8.90E-4 ID:SGTC_3026|Compound:9090633|FD-Score:3.72|P-value:1.93E-4 ID:SGTC_3083|Compound:9117465|FD-Score:3.65|P-value:2.55E-4

Top fitness defect scores for YPR145W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1070 indatraline 7.37 8.8 µM 1.03E-12 60S ribosome export 2 8.83E-14
2 SGTC_1215 3852-0577 5.91 11.2 µM 8.62E-9 4 1.73E-9
3 SGTC_2591 skatole 5.24 100.0 µM 2.87E-7 8 7.98E-8
4 SGTC_1085 doxorubicin 5.07 7.7 µM 6.76E-7 anthracycline transcription coupled DNA repair 4 2.04E-7
5 SGTC_2884 9058502 5.04 52.0 µM 7.60E-7 11 2.32E-7
6 SGTC_2905 7799065 4.98 20.1 µM 1.02E-6 5 3.18E-7
7 SGTC_1994 st072947 4.97 40.9 µM 1.08E-6 6 3.39E-7
8 SGTC_1256 0570-0116 4.87 285.0 µM 1.72E-6 4 5.66E-7
9 SGTC_1068 loratadine 4.83 26.9 µM 2.08E-6 12 6.97E-7
10 SGTC_2120 5538279 4.72 152.0 µM 3.43E-6 7 1.20E-6
11 SGTC_956 1185-0240 4.43 191.0 µM 1.20E-5 14 4.70E-6
12 SGTC_2969 9091408 4.41 28.7 µM 1.31E-5 16 5.21E-6
13 SGTC_2445 5742597 4.32 35.6 µM 1.92E-5 28 7.90E-6
14 SGTC_2394 5221648 4.23 200.0 µM 2.80E-5 18 1.19E-5
15 SGTC_1824 st053723 4.14 68.6 µM 3.90E-5 27 1.71E-5
16 SGTC_1052 3253-3998 3.90 198.0 µM 9.93E-5 19 4.76E-5
17 SGTC_394 menadione 3.80 8.3 µM 1.45E-4 superoxide 37 7.18E-5
18 SGTC_1989 st071868 3.78 50.2 µM 1.59E-4 13 7.92E-5
19 SGTC_217 ketoconazole 3.75 989.0 nM 1.73E-4 ergosterol depletion effects on membrane 30 8.73E-5
20 SGTC_738 1315-0003 3.72 72.4 µM 1.93E-4 28 9.84E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.092 1.00E-7 YGR066C_p YGR066C_p hom Putative protein of unknown function
0.087 4.16E-7 YMR271C URA10 hom Minor orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in the de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA10 has a paralog, URA5, that arose from the whole genome duplication
0.084 1.05E-6 YHR016C YSC84 hom Actin-binding protein; involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p; YSC84 has a paralog, LSB3, that arose from the whole genome duplication
0.083 1.41E-6 YDR192C NUP42 hom FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; interacts with Gle1p
0.080 3.79E-6 YNL272C SEC2 het Guanyl-nucleotide exchange factor for the small G-protein Sec4p; essential for post-Golgi vesicle transport and for autophagy; associates with the exocyst, via exocyst subunit Sec15p, on secretory vesicles
0.077 7.94E-6 YBL099W ATP1 hom Alpha subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated
0.077 8.78E-6 YLR078C BOS1 het v-SNARE (vesicle specific SNAP receptor), localized to the endoplasmic reticulum membrane and necessary for vesicular transport from the ER to the Golgi
0.075 1.29E-5 YDR189W SLY1 het Hydrophilic protein involved in vesicle trafficking between the ER and Golgi; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex
0.074 1.73E-5 YAL044W-A_p YAL044W-A_p hom Putative protein of unknown function; similar to S. pombe uvi31 which is a putative DNA repair protein
0.074 1.85E-5 YDR472W TRS31 het One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
0.073 2.57E-5 YGR097W ASK10 hom Component of RNA polymerase II holoenzyme; phosphorylated in response to oxidative stress; has a role in destruction of Ssn8p; proposed to function in activation of the glycerol channel Fps1p; ASK10 has a paralog, RGC1, that arose from the whole genome duplication
0.070 4.58E-5 YOR346W REV1 hom Deoxycytidyl transferase; involved in repair of abasic sites and adducted guanines in damaged DNA by translesion synthesis (TLS); forms a complex with the subunits of DNA polymerase zeta, Rev3p and Rev7p; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.070 5.24E-5 YHR043C DOG2 hom 2-deoxyglucose-6-phosphate phosphatase; member of a family of low molecular weight phosphatases, paralogous to DOG1, induced by oxidative and osmotic stress, confers 2-deoxyglucose resistance when overexpressed
0.069 6.86E-5 YER173W RAD24 hom Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; subunit of a clamp loader that loads Rad17p-Mec3p-Ddc1p onto DNA; homolog of human and S. pombe Rad17 protein
0.069 6.91E-5 YML009C-A_d YML009C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data