0258-0098

6-[(4-methoxy-2-nitroanilino)methylidene]-3-nitrocyclohexa-2,4-dien-1-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1
Screen concentration 57.4 μM
Source ChemDiv (Drug-like library)
PubChem CID 6790032
SMILES COC1=CC(=C(C=C1)NC=C2C=CC(=CC2=O)[N+](=O)[O-])[N+](=O)[O-]
Standardized SMILES COc1ccc(N=Cc2ccc(cc2O)[N+](=O)[O-])c(c1)[N+](=O)[O-]
Molecular weight 317.2536
ALogP 1.72
H-bond donor count 1
H-bond acceptor count 7
Response signature heme biosynthesis & mitochondrial translocase

Pool Growth Kinetics
% growth inhibition (Het. pool) 37.12
% growth inhibition (Hom. pool) 20.47


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6790032
Download HIP data (tab-delimited text)  (excel)
Gene:ARC19(YKL013C)|FD-Score:-3.13|P-value:8.89E-4|Clearance:0||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARP4(YJL081C)|FD-Score:4.59|P-value:2.26E-6|Clearance:0.12||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:CAK1(YFL029C)|FD-Score:-3.95|P-value:3.86E-5|Clearance:0||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:CDC36(YDL165W)|FD-Score:-3.72|P-value:9.91E-5|Clearance:0||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:DAD1(YDR016C)|FD-Score:5.7|P-value:6.05E-9|Clearance:0.03||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:DBP2(YNL112W)|FD-Score:8.28|P-value:6.38E-17|Clearance:1.83||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:DBP5(YOR046C)|FD-Score:3.54|P-value:2.02E-4|Clearance:0.17||SGD DESC:Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family; involved in mRNA export from the nucleus; involved in translation termination; ATP/ADP cycling is regulated by Gle1p and Nup159p Gene:DRS1(YLL008W)|FD-Score:-4.29|P-value:8.75E-6|Clearance:0||SGD DESC:Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles Gene:FAS1(YKL182W)|FD-Score:8.43|P-value:1.71E-17|Clearance:0.16||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:GLC7(YER133W)|FD-Score:-3.62|P-value:1.46E-4|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:HEM12(YDR047W)|FD-Score:27.3|P-value:3.41E-164|Clearance:18.85||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:HYP2(YEL034W)|FD-Score:5.65|P-value:8.23E-9|Clearance:0.35||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:MED6(YHR058C)|FD-Score:-3.83|P-value:6.31E-5|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress Gene:MOT1(YPL082C)|FD-Score:6.21|P-value:2.67E-10|Clearance:0.14||SGD DESC:Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA Gene:NUP192(YJL039C)|FD-Score:6.07|P-value:6.49E-10|Clearance:0.28||SGD DESC:Essential subunit of the inner ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport; homologous to human NUP205 Gene:NUP85(YJR042W)|FD-Score:-4.2|P-value:1.32E-5|Clearance:0||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75 Gene:RPL10(YLR075W)|FD-Score:4.02|P-value:2.95E-5|Clearance:0.04||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPM2(YML091C)|FD-Score:3.97|P-value:3.53E-5|Clearance:0.18||SGD DESC:Protein subunit of mitochondrial RNase P, has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus Gene:RPN3(YER021W)|FD-Score:3.75|P-value:8.75E-5|Clearance:0.05||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RSC4(YKR008W)|FD-Score:5.3|P-value:5.86E-8|Clearance:0.2||SGD DESC:Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p Gene:RSC8(YFR037C)|FD-Score:3.79|P-value:7.41E-5|Clearance:0.04||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RSC9(YML127W)|FD-Score:5.79|P-value:3.59E-9|Clearance:0.09||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:RSP5(YER125W)|FD-Score:6.45|P-value:5.56E-11|Clearance:0.24||SGD DESC:E3 ubiquitin ligase of the NEDD4 family; involved in regulating many cellular processes including MVB sorting, heat shock response, transcription, endocytosis, and ribosome stability; human homolog is involved in Liddle syndrome; mutant tolerates aneuploidy; ubiquitylates Sec23p Gene:SFH1(YLR321C)|FD-Score:3.71|P-value:1.06E-4|Clearance:0.17||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:SNP1(YIL061C)|FD-Score:3.37|P-value:3.78E-4|Clearance:0.14||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress Gene:STH1(YIL126W)|FD-Score:5.66|P-value:7.38E-9|Clearance:0.02||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:TIF35(YDR429C)|FD-Score:-3.48|P-value:2.53E-4|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:UTP6(YDR449C)|FD-Score:5.1|P-value:1.68E-7|Clearance:0.52||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YDR396W(YDR396W_d)|FD-Score:4.05|P-value:2.55E-5|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; extensively overlaps essential NCB2 gene encoding the beta subunit of the NC2 dimeric histone-fold complex Gene:YGR114C(YGR114C_d)|FD-Score:3.09|P-value:9.89E-4|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YGR115C(YGR115C_d)|FD-Score:3.18|P-value:7.31E-4|Clearance:0.09||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YNL114C(YNL114C_d)|FD-Score:4.47|P-value:3.96E-6|Clearance:0.4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit Gene:YNL150W(YNL150W_d)|FD-Score:3.22|P-value:6.33E-4|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking Gene:YRA1(YDR381W)|FD-Score:4.07|P-value:2.34E-5|Clearance:0.02||SGD DESC:RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member Gene:ARC19(YKL013C)|FD-Score:-3.13|P-value:8.89E-4|Clearance:0||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARP4(YJL081C)|FD-Score:4.59|P-value:2.26E-6|Clearance:0.12||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:CAK1(YFL029C)|FD-Score:-3.95|P-value:3.86E-5|Clearance:0||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:CDC36(YDL165W)|FD-Score:-3.72|P-value:9.91E-5|Clearance:0||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:DAD1(YDR016C)|FD-Score:5.7|P-value:6.05E-9|Clearance:0.03||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:DBP2(YNL112W)|FD-Score:8.28|P-value:6.38E-17|Clearance:1.83||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:DBP5(YOR046C)|FD-Score:3.54|P-value:2.02E-4|Clearance:0.17||SGD DESC:Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family; involved in mRNA export from the nucleus; involved in translation termination; ATP/ADP cycling is regulated by Gle1p and Nup159p Gene:DRS1(YLL008W)|FD-Score:-4.29|P-value:8.75E-6|Clearance:0||SGD DESC:Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles Gene:FAS1(YKL182W)|FD-Score:8.43|P-value:1.71E-17|Clearance:0.16||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:GLC7(YER133W)|FD-Score:-3.62|P-value:1.46E-4|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:HEM12(YDR047W)|FD-Score:27.3|P-value:3.41E-164|Clearance:18.85||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:HYP2(YEL034W)|FD-Score:5.65|P-value:8.23E-9|Clearance:0.35||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:MED6(YHR058C)|FD-Score:-3.83|P-value:6.31E-5|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress Gene:MOT1(YPL082C)|FD-Score:6.21|P-value:2.67E-10|Clearance:0.14||SGD DESC:Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA Gene:NUP192(YJL039C)|FD-Score:6.07|P-value:6.49E-10|Clearance:0.28||SGD DESC:Essential subunit of the inner ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport; homologous to human NUP205 Gene:NUP85(YJR042W)|FD-Score:-4.2|P-value:1.32E-5|Clearance:0||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75 Gene:RPL10(YLR075W)|FD-Score:4.02|P-value:2.95E-5|Clearance:0.04||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPM2(YML091C)|FD-Score:3.97|P-value:3.53E-5|Clearance:0.18||SGD DESC:Protein subunit of mitochondrial RNase P, has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus Gene:RPN3(YER021W)|FD-Score:3.75|P-value:8.75E-5|Clearance:0.05||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RSC4(YKR008W)|FD-Score:5.3|P-value:5.86E-8|Clearance:0.2||SGD DESC:Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p Gene:RSC8(YFR037C)|FD-Score:3.79|P-value:7.41E-5|Clearance:0.04||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RSC9(YML127W)|FD-Score:5.79|P-value:3.59E-9|Clearance:0.09||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:RSP5(YER125W)|FD-Score:6.45|P-value:5.56E-11|Clearance:0.24||SGD DESC:E3 ubiquitin ligase of the NEDD4 family; involved in regulating many cellular processes including MVB sorting, heat shock response, transcription, endocytosis, and ribosome stability; human homolog is involved in Liddle syndrome; mutant tolerates aneuploidy; ubiquitylates Sec23p Gene:SFH1(YLR321C)|FD-Score:3.71|P-value:1.06E-4|Clearance:0.17||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:SNP1(YIL061C)|FD-Score:3.37|P-value:3.78E-4|Clearance:0.14||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress Gene:STH1(YIL126W)|FD-Score:5.66|P-value:7.38E-9|Clearance:0.02||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:TIF35(YDR429C)|FD-Score:-3.48|P-value:2.53E-4|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:UTP6(YDR449C)|FD-Score:5.1|P-value:1.68E-7|Clearance:0.52||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YDR396W(YDR396W_d)|FD-Score:4.05|P-value:2.55E-5|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; extensively overlaps essential NCB2 gene encoding the beta subunit of the NC2 dimeric histone-fold complex Gene:YGR114C(YGR114C_d)|FD-Score:3.09|P-value:9.89E-4|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YGR115C(YGR115C_d)|FD-Score:3.18|P-value:7.31E-4|Clearance:0.09||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YNL114C(YNL114C_d)|FD-Score:4.47|P-value:3.96E-6|Clearance:0.4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit Gene:YNL150W(YNL150W_d)|FD-Score:3.22|P-value:6.33E-4|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking Gene:YRA1(YDR381W)|FD-Score:4.07|P-value:2.34E-5|Clearance:0.02||SGD DESC:RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6790032
Download HOP data (tab-delimited text)  (excel)
Gene:APD1(YBR151W)|FD-Score:6.75|P-value:7.30E-12||SGD DESC:Protein of unknown function, required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus Gene:ARP6(YLR085C)|FD-Score:-3.1|P-value:9.82E-4||SGD DESC:Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:AXL1(YPR122W)|FD-Score:3.19|P-value:7.00E-4||SGD DESC:Haploid specific endoprotease that performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells Gene:BUG1(YDL099W)|FD-Score:3.76|P-value:8.36E-5||SGD DESC:Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes Gene:CBP1(YJL209W)|FD-Score:3.09|P-value:9.93E-4||SGD DESC:Mitochondrial protein, regulator of COB mRNA stability and translation; interacts with the 5'-untranslated region of the COB mRNA; found in a complex at the inner membrane along with Pet309p; localizes to mitochondrial foci upon DNA replication stress Gene:CGI121(YML036W)|FD-Score:-3.11|P-value:9.45E-4||SGD DESC:Component of the EKC/KEOPS complex with Bud32p, Kae1p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription; Cgi121p is dispensable for tRNA modification Gene:COG6(YNL041C)|FD-Score:5.73|P-value:5.08E-9||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:6.85|P-value:3.80E-12||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:6.72|P-value:9.24E-12||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTF8(YHR191C)|FD-Score:3.19|P-value:7.11E-4||SGD DESC:Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion Gene:DAP1(YPL170W)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis Gene:ELA1(YNL230C)|FD-Score:-4.39|P-value:5.65E-6||SGD DESC:Elongin A; F-box protein that forms a heterodimer with Elc1p and is required for ubiquitin-dependent degradation of the RNA Polymerase II subunit Rpo21p; subunit of the Elongin-Cullin-Socs (ECS) ligase complex Gene:ERG5(YMR015C)|FD-Score:-3.65|P-value:1.31E-4||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:FAU1(YER183C)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:GCS1(YDL226C)|FD-Score:3.56|P-value:1.82E-4||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:HUL4(YJR036C)|FD-Score:-3.17|P-value:7.75E-4||SGD DESC:Protein with similarity to hect domain E3 ubiquitin-protein ligases; not essential for viability; found in association with Trf4 in TRAMP complex Gene:IRS4(YKR019C)|FD-Score:4.84|P-value:6.39E-7||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KAR5(YMR065W)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Protein required for nuclear membrane fusion during karyogamy, localizes to the membrane with a soluble portion in the endoplasmic reticulum lumen, may form a complex with Jem1p and Kar2p; expression of the gene is regulated by pheromone Gene:MRS4(YKR052C)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Iron transporter of the mitochondrial carrier family; mediates Fe2+ transport across the inner mitochondrial membrane; active under low-iron conditions; may transport other cations; protein abundance increases in response to DNA replication stress; MRS4 has a paralog, MRS3, that arose from the whole genome duplication Gene:NEM1(YHR004C)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard Gene:NHX1(YDR456W)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion Gene:OPI3(YJR073C)|FD-Score:-3.21|P-value:6.70E-4||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:ORM2(YLR350W)|FD-Score:3.12|P-value:8.98E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress Gene:PIB1(YDR313C)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:RING-type ubiquitin ligase of the endosomal and vacuolar membranes, binds phosphatidylinositol(3)-phosphate; contains a FYVE finger domain Gene:PPM1(YDR435C)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits Gene:RIM8(YGL045W)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:SLZ1(YNL196C_p)|FD-Score:3.19|P-value:7.11E-4||SGD DESC:Sporulation-specific protein with a leucine zipper motif Gene:SOK1(YDR006C)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Protein whose overexpression suppresses the growth defect of mutants lacking protein kinase A activity; involved in cAMP-mediated signaling; localized to the nucleus; similar to the mouse testis-specific protein PBS13 Gene:SPF1(YEL031W)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SPT21(YMR179W)|FD-Score:-4.13|P-value:1.81E-5||SGD DESC:Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SPT3(YDR392W)|FD-Score:-3.32|P-value:4.57E-4||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:TOM71(YHR117W)|FD-Score:-3.31|P-value:4.65E-4||SGD DESC:Mitochondrial outer membrane protein; probable minor component of the TOM (translocase of outer membrane) complex responsible for recognition and import of mitochondrially directed proteins; TOM71 has a paralog, TOM70, that arose from the whole genome duplication Gene:VPS21(YOR089C)|FD-Score:4.06|P-value:2.46E-5||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS61(YDR136C_d)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS8(YAL002W)|FD-Score:3.56|P-value:1.82E-4||SGD DESC:Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif Gene:YCR095W-A(YCR095W-A_p)|FD-Score:3.35|P-value:4.06E-4||SGD DESC:Putative protein of unknown function Gene:YFL012W(YFL012W_p)|FD-Score:3.18|P-value:7.27E-4||SGD DESC:Putative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin Gene:YGR137W(YGR137W_d)|FD-Score:3.11|P-value:9.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR045W(YHR045W_p)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Putative protein of unknown function; possible role in iron metabolism and/or amino acid and carbohydrate metabolism; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum Gene:YHR097C(YHR097C_p)|FD-Score:-3.21|P-value:6.58E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus Gene:YIR035C(YIR035C_p)|FD-Score:3.13|P-value:8.75E-4||SGD DESC:Putative cytoplasmic short-chain dehydrogenase/reductase Gene:YLR312C(YLR312C_p)|FD-Score:-3.33|P-value:4.28E-4||SGD DESC:Putative protein of unknown function Gene:YMR209C(YMR209C_p)|FD-Score:4.28|P-value:9.52E-6||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; YMR209C is not an essential gene Gene:YMR317W(YMR317W_p)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Putative protein of unknown function with some similarity to sialidase from Trypanosoma; YMR317W is not an essential gene Gene:YNL179C(YNL179C_d)|FD-Score:3.8|P-value:7.25E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; deletion in cyr1 mutant results in loss of stress resistance Gene:YOR314W(YOR314W_d)|FD-Score:3.25|P-value:5.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPK1(YKL126W)|FD-Score:-3.49|P-value:2.46E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YTA7(YGR270W)|FD-Score:8.43|P-value:1.66E-17||SGD DESC:Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p Gene:APD1(YBR151W)|FD-Score:6.75|P-value:7.30E-12||SGD DESC:Protein of unknown function, required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus Gene:ARP6(YLR085C)|FD-Score:-3.1|P-value:9.82E-4||SGD DESC:Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:AXL1(YPR122W)|FD-Score:3.19|P-value:7.00E-4||SGD DESC:Haploid specific endoprotease that performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells Gene:BUG1(YDL099W)|FD-Score:3.76|P-value:8.36E-5||SGD DESC:Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes Gene:CBP1(YJL209W)|FD-Score:3.09|P-value:9.93E-4||SGD DESC:Mitochondrial protein, regulator of COB mRNA stability and translation; interacts with the 5'-untranslated region of the COB mRNA; found in a complex at the inner membrane along with Pet309p; localizes to mitochondrial foci upon DNA replication stress Gene:CGI121(YML036W)|FD-Score:-3.11|P-value:9.45E-4||SGD DESC:Component of the EKC/KEOPS complex with Bud32p, Kae1p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription; Cgi121p is dispensable for tRNA modification Gene:COG6(YNL041C)|FD-Score:5.73|P-value:5.08E-9||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:6.85|P-value:3.80E-12||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:6.72|P-value:9.24E-12||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTF8(YHR191C)|FD-Score:3.19|P-value:7.11E-4||SGD DESC:Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion Gene:DAP1(YPL170W)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis Gene:ELA1(YNL230C)|FD-Score:-4.39|P-value:5.65E-6||SGD DESC:Elongin A; F-box protein that forms a heterodimer with Elc1p and is required for ubiquitin-dependent degradation of the RNA Polymerase II subunit Rpo21p; subunit of the Elongin-Cullin-Socs (ECS) ligase complex Gene:ERG5(YMR015C)|FD-Score:-3.65|P-value:1.31E-4||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:FAU1(YER183C)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:GCS1(YDL226C)|FD-Score:3.56|P-value:1.82E-4||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:HUL4(YJR036C)|FD-Score:-3.17|P-value:7.75E-4||SGD DESC:Protein with similarity to hect domain E3 ubiquitin-protein ligases; not essential for viability; found in association with Trf4 in TRAMP complex Gene:IRS4(YKR019C)|FD-Score:4.84|P-value:6.39E-7||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KAR5(YMR065W)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Protein required for nuclear membrane fusion during karyogamy, localizes to the membrane with a soluble portion in the endoplasmic reticulum lumen, may form a complex with Jem1p and Kar2p; expression of the gene is regulated by pheromone Gene:MRS4(YKR052C)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Iron transporter of the mitochondrial carrier family; mediates Fe2+ transport across the inner mitochondrial membrane; active under low-iron conditions; may transport other cations; protein abundance increases in response to DNA replication stress; MRS4 has a paralog, MRS3, that arose from the whole genome duplication Gene:NEM1(YHR004C)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard Gene:NHX1(YDR456W)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion Gene:OPI3(YJR073C)|FD-Score:-3.21|P-value:6.70E-4||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:ORM2(YLR350W)|FD-Score:3.12|P-value:8.98E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress Gene:PIB1(YDR313C)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:RING-type ubiquitin ligase of the endosomal and vacuolar membranes, binds phosphatidylinositol(3)-phosphate; contains a FYVE finger domain Gene:PPM1(YDR435C)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits Gene:RIM8(YGL045W)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:SLZ1(YNL196C_p)|FD-Score:3.19|P-value:7.11E-4||SGD DESC:Sporulation-specific protein with a leucine zipper motif Gene:SOK1(YDR006C)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Protein whose overexpression suppresses the growth defect of mutants lacking protein kinase A activity; involved in cAMP-mediated signaling; localized to the nucleus; similar to the mouse testis-specific protein PBS13 Gene:SPF1(YEL031W)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SPT21(YMR179W)|FD-Score:-4.13|P-value:1.81E-5||SGD DESC:Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SPT3(YDR392W)|FD-Score:-3.32|P-value:4.57E-4||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:TOM71(YHR117W)|FD-Score:-3.31|P-value:4.65E-4||SGD DESC:Mitochondrial outer membrane protein; probable minor component of the TOM (translocase of outer membrane) complex responsible for recognition and import of mitochondrially directed proteins; TOM71 has a paralog, TOM70, that arose from the whole genome duplication Gene:VPS21(YOR089C)|FD-Score:4.06|P-value:2.46E-5||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS61(YDR136C_d)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS8(YAL002W)|FD-Score:3.56|P-value:1.82E-4||SGD DESC:Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif Gene:YCR095W-A(YCR095W-A_p)|FD-Score:3.35|P-value:4.06E-4||SGD DESC:Putative protein of unknown function Gene:YFL012W(YFL012W_p)|FD-Score:3.18|P-value:7.27E-4||SGD DESC:Putative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin Gene:YGR137W(YGR137W_d)|FD-Score:3.11|P-value:9.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR045W(YHR045W_p)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Putative protein of unknown function; possible role in iron metabolism and/or amino acid and carbohydrate metabolism; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum Gene:YHR097C(YHR097C_p)|FD-Score:-3.21|P-value:6.58E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus Gene:YIR035C(YIR035C_p)|FD-Score:3.13|P-value:8.75E-4||SGD DESC:Putative cytoplasmic short-chain dehydrogenase/reductase Gene:YLR312C(YLR312C_p)|FD-Score:-3.33|P-value:4.28E-4||SGD DESC:Putative protein of unknown function Gene:YMR209C(YMR209C_p)|FD-Score:4.28|P-value:9.52E-6||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; YMR209C is not an essential gene Gene:YMR317W(YMR317W_p)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Putative protein of unknown function with some similarity to sialidase from Trypanosoma; YMR317W is not an essential gene Gene:YNL179C(YNL179C_d)|FD-Score:3.8|P-value:7.25E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; deletion in cyr1 mutant results in loss of stress resistance Gene:YOR314W(YOR314W_d)|FD-Score:3.25|P-value:5.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPK1(YKL126W)|FD-Score:-3.49|P-value:2.46E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YTA7(YGR270W)|FD-Score:8.43|P-value:1.66E-17||SGD DESC:Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR047W27.303.41E-16418.90HEM12Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
YKL182W8.431.71E-170.15FAS1Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities
YNL112W8.286.38E-171.83DBP2ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites
YER125W6.455.56E-110.24RSP5E3 ubiquitin ligase of the NEDD4 family; involved in regulating many cellular processes including MVB sorting, heat shock response, transcription, endocytosis, and ribosome stability; human homolog is involved in Liddle syndrome; mutant tolerates aneuploidy; ubiquitylates Sec23p
YPL082C6.212.67E-100.14MOT1Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA
YJL039C6.076.49E-100.28NUP192Essential subunit of the inner ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport; homologous to human NUP205
YML127W5.793.59E-90.09RSC9Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway
YDR016C5.706.05E-90.03DAD1Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
YIL126W5.667.38E-90.02STH1ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p
YEL034W5.658.23E-90.35HYP2Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication
YKR008W5.305.86E-80.20RSC4Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p
YDR449C5.101.68E-70.52UTP6Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YJL081C4.592.26E-60.12ARP4Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
YNL114C_d4.473.96E-60.40YNL114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit
YDR381W4.072.34E-50.02YRA1RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGR270W8.431.66E-17YTA7Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p
YGL005C6.853.80E-12COG7Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YBR151W6.757.30E-12APD1Protein of unknown function, required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus
YML071C6.729.24E-12COG8Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YNL041C5.735.08E-9COG6Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YKR019C4.846.39E-7IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
YMR209C_p4.289.52E-6YMR209C_pPutative S-adenosylmethionine-dependent methyltransferase; YMR209C is not an essential gene
YOR089C4.062.46E-5VPS21Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication
YNL179C_d3.807.25E-5YNL179C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; deletion in cyr1 mutant results in loss of stress resistance
YDL099W3.768.36E-5BUG1Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes
YDR313C3.661.24E-4PIB1RING-type ubiquitin ligase of the endosomal and vacuolar membranes, binds phosphatidylinositol(3)-phosphate; contains a FYVE finger domain
YDR435C3.661.26E-4PPM1Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits
YPL170W3.641.36E-4DAP1Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis
YKR052C3.601.59E-4MRS4Iron transporter of the mitochondrial carrier family; mediates Fe2+ transport across the inner mitochondrial membrane; active under low-iron conditions; may transport other cations; protein abundance increases in response to DNA replication stress; MRS4 has a paralog, MRS3, that arose from the whole genome duplication
YDR006C3.601.60E-4SOK1Protein whose overexpression suppresses the growth defect of mutants lacking protein kinase A activity; involved in cAMP-mediated signaling; localized to the nucleus; similar to the mouse testis-specific protein PBS13

GO enrichment analysis for SGTC_1
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.4151.30E-240SGTC_273hydroxyurea 18.1 mMMiscellaneous36570.0384615heme biosynthesis & mitochondrial translocase
0.2932.71E-115SGTC_1113artemisinin 1.2 μMTimTec (Natural product library)22400.0384615heme biosynthesis & mitochondrial translocase
0.2782.33E-103SGTC_203k066-0004 30.9 μMChemDiv (Drug-like library)67954120.166667RSC & ERG11
0.2446.64E-80SGTC_1098clofazimine 15.1 μMNIH Clinical Collection27940.0568182heme biosynthesis & mitochondrial translocase
0.2202.06E-64SGTC_2617dihydrotanshinone i 20.8 μMMicrosource (Natural product library)53167430.0649351copper-dependent oxidative stress
0.2137.89E-61SGTC_23369001819 198.7 μMChembridge (Fragment library)29896020.0909091heme biosynthesis & mitochondrial translocase
0.2103.33E-59SGTC_260302-0163 425.2 μMChemDiv (Drug-like library)31009150.333333heme biosynthesis & mitochondrial translocase
0.2103.37E-59SGTC_2519cryptotanshinone 88.0 μMMicrosource (Natural product library)1602540.0617284heme biosynthesis & mitochondrial translocase
0.2054.13E-56SGTC_2766oltipraz 83.9 μMMiscellaneous473180.0441176heme requiring
0.2045.59E-56SGTC_8420271-0008 11.1 μMChemDiv (Drug-like library)12217090.0769231heme biosynthesis & mitochondrial translocase
0.2021.21E-54SGTC_2881315-0019 35.1 μMChemDiv (Drug-like library)68114740.227273RSC & ERG11
0.1916.75E-49SGTC_208k072-0098 12.4 μMChemDiv (Drug-like library)59489010.102941heme biosynthesis & mitochondrial translocase
0.1918.21E-49SGTC_22256654152 15.4 μMChembridge (Fragment library)975820.0655738
0.1881.65E-47SGTC_3191348-1596 45.5 μMChemDiv (Drug-like library)53805050.174603RSC & ERG11
0.1881.69E-47SGTC_331k048-0133 24.5 μMChemDiv (Drug-like library)68135820.179104RSC & ERG11

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_322k048-013619.3 μM0.4915256823282ChemDiv (Drug-like library)430.04822.88215RSC & ERG11
SGTC_6781218-217985.9 μM0.4310346820081ChemDiv (Drug-like library)272.256081.82515RNA pol III & RNase P/MRP
SGTC_5571315-011016.2 μM0.3666676833801ChemDiv (Drug-like library)509.077562.63114Golgi
SGTC_24025553605200 μM0.3611116758546Miscellaneous373.36153.77816
SGTC_4260988-003714.6 μM0.3559325383008ChemDiv (Drug-like library)257.244741.51425ERAD & cell cycle
SGTC_7680384-003377.3 μM0.3392863965305ChemDiv (Drug-like library)275.216962.43216fatty acid desaturase (OLE1)
SGTC_260302-0163425.16 μM0.3333333100915ChemDiv (Drug-like library)275.216962.92626heme biosynthesis & mitochondrial translocase
SGTC_8890919-152482.7 μM0.3333333905452ChemDiv (Drug-like library)274.271962.85825
SGTC_1731st03596463 μM0.3333336739575TimTec (Natural product derivative library)317.253640.26547
SGTC_2842900067519.48 μM0.3278692989072Chembridge (Drug-like library)258.272563.114