4049-0218

N-(3,4-dimethylphenyl)-4-fluorobenzenesulfonamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1003
Screen concentration 100.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 295161
SMILES CC1=C(C=C(C=C1)NS(=O)(=O)C2=CC=C(C=C2)F)C
Standardized SMILES Cc1ccc(NS(=O)(=O)c2ccc(F)cc2)cc1C
Molecular weight 279.3299
ALogP 3.5
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 31.44
% growth inhibition (Hom. pool) 8.91


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 295161
Download HIP data (tab-delimited text)  (excel)
Gene:ACT1(YFL039C)|FD-Score:3.23|P-value:6.21E-4|Clearance:0.01||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:ALG1(YBR110W)|FD-Score:3.18|P-value:7.44E-4|Clearance:0.05||SGD DESC:Mannosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum (ER); essential for viability, mutation is functionally complemented by human ortholog Gene:ALG2(YGL065C)|FD-Score:-3.84|P-value:6.21E-5|Clearance:0||SGD DESC:Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol Gene:BRN1(YBL097W)|FD-Score:5.58|P-value:1.23E-8|Clearance:0.51||SGD DESC:Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission Gene:CCT3(YJL014W)|FD-Score:5.13|P-value:1.44E-7|Clearance:0.51||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:DBP2(YNL112W)|FD-Score:4.67|P-value:1.50E-6|Clearance:0.51||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:GNA1(YFL017C)|FD-Score:3.13|P-value:8.82E-4|Clearance:0.03||SGD DESC:Evolutionarily conserved glucosamine-6-phosphate acetyltransferase required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA Gene:HYM1(YKL189W)|FD-Score:-3.63|P-value:1.44E-4|Clearance:0||SGD DESC:Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with Kic1p and Sog2p, localizes to sites of polarized growth during budding and during the mating response Gene:HYP2(YEL034W)|FD-Score:4.67|P-value:1.53E-6|Clearance:0.51||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:INN1(YNL152W)|FD-Score:3.35|P-value:4.06E-4|Clearance:0.02||SGD DESC:Essential protein that associates with contractile actomyosin ring; required for ingression of the plasma membrane into the bud neck during cytokinesis; C2 domain, a membrane targeting module, is required for function; activates chitin synthase activity of Chs2p during cytokinesis Gene:IRR1(YIL026C)|FD-Score:-3.23|P-value:6.16E-4|Clearance:0||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:LSM3(YLR438C-A)|FD-Score:-3.35|P-value:4.06E-4|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MAS1(YLR163C)|FD-Score:4.87|P-value:5.67E-7|Clearance:0.51||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:MED6(YHR058C)|FD-Score:-5.68|P-value:6.61E-9|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress Gene:MGE1(YOR232W)|FD-Score:-3.14|P-value:8.53E-4|Clearance:0||SGD DESC:Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE Gene:NIP1(YMR309C)|FD-Score:3.4|P-value:3.39E-4|Clearance:0.05||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:NOP9(YJL010C)|FD-Score:3.57|P-value:1.78E-4|Clearance:0.01||SGD DESC:Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain Gene:NRD1(YNL251C)|FD-Score:-3.31|P-value:4.59E-4|Clearance:0||SGD DESC:RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with the C-terminal domain of the RNA polymerase II large subunit (Rpo21p), preferentially at phosphorylated Ser5, to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites Gene:NUS1(YDL193W)|FD-Score:3.4|P-value:3.33E-4|Clearance:0.01||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:POP3(YNL282W)|FD-Score:3.59|P-value:1.68E-4|Clearance:0.02||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRE7(YBL041W)|FD-Score:-3.6|P-value:1.59E-4|Clearance:0||SGD DESC:Beta 6 subunit of the 20S proteasome Gene:RPB7(YDR404C)|FD-Score:3.1|P-value:9.73E-4|Clearance:0.02||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPB8(YOR224C)|FD-Score:3.78|P-value:7.95E-5|Clearance:0.09||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPT1(YKL145W)|FD-Score:3.68|P-value:1.14E-4|Clearance:0.06||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:RSC3(YDR303C)|FD-Score:3.27|P-value:5.29E-4|Clearance:0.01||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for maintenance of proper ploidy and regulation of ribosomal protein genes and the cell wall/stress response; highly similar to Rsc30p Gene:SCL1(YGL011C)|FD-Score:-3.36|P-value:3.89E-4|Clearance:0||SGD DESC:Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria Gene:SEC7(YDR170C)|FD-Score:-3.45|P-value:2.77E-4|Clearance:0||SGD DESC:Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles Gene:SMC3(YJL074C)|FD-Score:-3.25|P-value:5.71E-4|Clearance:0||SGD DESC:Subunit of the multiprotein cohesin complex required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member Gene:SPN1(YPR133C)|FD-Score:3.41|P-value:3.31E-4|Clearance:0||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:SRP1(YNL189W)|FD-Score:-4.23|P-value:1.18E-5|Clearance:0||SGD DESC:Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation Gene:TAF12(YDR145W)|FD-Score:4.29|P-value:9.07E-6|Clearance:0.51||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TIF35(YDR429C)|FD-Score:3.26|P-value:5.57E-4|Clearance:0.03||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TOA1(YOR194C)|FD-Score:5.77|P-value:4.03E-9|Clearance:0.51||SGD DESC:TFIIA large subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to largest and second largest subunits of human and Drosophila TFIIA Gene:TRM5(YHR070W)|FD-Score:-3.5|P-value:2.32E-4|Clearance:0||SGD DESC:tRNA(m(1)G37)methyltransferase, methylates a tRNA base adjacent to the anticodon that has a role in prevention of frameshifting; highly conserved across Archaea, Bacteria, and Eukarya Gene:TUB1(YML085C)|FD-Score:3.62|P-value:1.45E-4|Clearance:0.04||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:YMR290W-A(YMR290W-A_d)|FD-Score:3.33|P-value:4.41E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:YPL238C(YPL238C_d)|FD-Score:3.22|P-value:6.49E-4|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Gene:YTH1(YPR107C)|FD-Score:3.56|P-value:1.85E-4|Clearance:0.16||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:ACT1(YFL039C)|FD-Score:3.23|P-value:6.21E-4|Clearance:0.01||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:ALG1(YBR110W)|FD-Score:3.18|P-value:7.44E-4|Clearance:0.05||SGD DESC:Mannosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum (ER); essential for viability, mutation is functionally complemented by human ortholog Gene:ALG2(YGL065C)|FD-Score:-3.84|P-value:6.21E-5|Clearance:0||SGD DESC:Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol Gene:BRN1(YBL097W)|FD-Score:5.58|P-value:1.23E-8|Clearance:0.51||SGD DESC:Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission Gene:CCT3(YJL014W)|FD-Score:5.13|P-value:1.44E-7|Clearance:0.51||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:DBP2(YNL112W)|FD-Score:4.67|P-value:1.50E-6|Clearance:0.51||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:GNA1(YFL017C)|FD-Score:3.13|P-value:8.82E-4|Clearance:0.03||SGD DESC:Evolutionarily conserved glucosamine-6-phosphate acetyltransferase required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA Gene:HYM1(YKL189W)|FD-Score:-3.63|P-value:1.44E-4|Clearance:0||SGD DESC:Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with Kic1p and Sog2p, localizes to sites of polarized growth during budding and during the mating response Gene:HYP2(YEL034W)|FD-Score:4.67|P-value:1.53E-6|Clearance:0.51||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:INN1(YNL152W)|FD-Score:3.35|P-value:4.06E-4|Clearance:0.02||SGD DESC:Essential protein that associates with contractile actomyosin ring; required for ingression of the plasma membrane into the bud neck during cytokinesis; C2 domain, a membrane targeting module, is required for function; activates chitin synthase activity of Chs2p during cytokinesis Gene:IRR1(YIL026C)|FD-Score:-3.23|P-value:6.16E-4|Clearance:0||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:LSM3(YLR438C-A)|FD-Score:-3.35|P-value:4.06E-4|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MAS1(YLR163C)|FD-Score:4.87|P-value:5.67E-7|Clearance:0.51||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:MED6(YHR058C)|FD-Score:-5.68|P-value:6.61E-9|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress Gene:MGE1(YOR232W)|FD-Score:-3.14|P-value:8.53E-4|Clearance:0||SGD DESC:Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE Gene:NIP1(YMR309C)|FD-Score:3.4|P-value:3.39E-4|Clearance:0.05||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:NOP9(YJL010C)|FD-Score:3.57|P-value:1.78E-4|Clearance:0.01||SGD DESC:Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain Gene:NRD1(YNL251C)|FD-Score:-3.31|P-value:4.59E-4|Clearance:0||SGD DESC:RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with the C-terminal domain of the RNA polymerase II large subunit (Rpo21p), preferentially at phosphorylated Ser5, to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites Gene:NUS1(YDL193W)|FD-Score:3.4|P-value:3.33E-4|Clearance:0.01||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:POP3(YNL282W)|FD-Score:3.59|P-value:1.68E-4|Clearance:0.02||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRE7(YBL041W)|FD-Score:-3.6|P-value:1.59E-4|Clearance:0||SGD DESC:Beta 6 subunit of the 20S proteasome Gene:RPB7(YDR404C)|FD-Score:3.1|P-value:9.73E-4|Clearance:0.02||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPB8(YOR224C)|FD-Score:3.78|P-value:7.95E-5|Clearance:0.09||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPT1(YKL145W)|FD-Score:3.68|P-value:1.14E-4|Clearance:0.06||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:RSC3(YDR303C)|FD-Score:3.27|P-value:5.29E-4|Clearance:0.01||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for maintenance of proper ploidy and regulation of ribosomal protein genes and the cell wall/stress response; highly similar to Rsc30p Gene:SCL1(YGL011C)|FD-Score:-3.36|P-value:3.89E-4|Clearance:0||SGD DESC:Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria Gene:SEC7(YDR170C)|FD-Score:-3.45|P-value:2.77E-4|Clearance:0||SGD DESC:Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles Gene:SMC3(YJL074C)|FD-Score:-3.25|P-value:5.71E-4|Clearance:0||SGD DESC:Subunit of the multiprotein cohesin complex required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member Gene:SPN1(YPR133C)|FD-Score:3.41|P-value:3.31E-4|Clearance:0||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:SRP1(YNL189W)|FD-Score:-4.23|P-value:1.18E-5|Clearance:0||SGD DESC:Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation Gene:TAF12(YDR145W)|FD-Score:4.29|P-value:9.07E-6|Clearance:0.51||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TIF35(YDR429C)|FD-Score:3.26|P-value:5.57E-4|Clearance:0.03||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TOA1(YOR194C)|FD-Score:5.77|P-value:4.03E-9|Clearance:0.51||SGD DESC:TFIIA large subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to largest and second largest subunits of human and Drosophila TFIIA Gene:TRM5(YHR070W)|FD-Score:-3.5|P-value:2.32E-4|Clearance:0||SGD DESC:tRNA(m(1)G37)methyltransferase, methylates a tRNA base adjacent to the anticodon that has a role in prevention of frameshifting; highly conserved across Archaea, Bacteria, and Eukarya Gene:TUB1(YML085C)|FD-Score:3.62|P-value:1.45E-4|Clearance:0.04||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:YMR290W-A(YMR290W-A_d)|FD-Score:3.33|P-value:4.41E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:YPL238C(YPL238C_d)|FD-Score:3.22|P-value:6.49E-4|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Gene:YTH1(YPR107C)|FD-Score:3.56|P-value:1.85E-4|Clearance:0.16||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 295161
Download HOP data (tab-delimited text)  (excel)
Gene:ABZ1(YNR033W)|FD-Score:3.31|P-value:4.67E-4||SGD DESC:Para-aminobenzoate (PABA) synthase; has similarity to Escherichia coli PABA synthase components PabA and PabB; required for the synthesis of para-aminobenzoic acid, an important intermediate for folate and ubiquinone Q biosynthesis; protein abundance increases in response to DNA replication stress Gene:ADO1(YJR105W)|FD-Score:-4.11|P-value:1.95E-5||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:AIF1(YNR074C)|FD-Score:-3.39|P-value:3.54E-4||SGD DESC:Mitochondrial cell death effector that translocates to the nucleus in response to apoptotic stimuli, homolog of mammalian Apoptosis-Inducing Factor, putative reductase Gene:ALR2(YFL050C)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Probable Mg(2+) transporter; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions; plays a role in regulating Ty1 transposition Gene:AMD2(YDR242W)|FD-Score:3.8|P-value:7.18E-5||SGD DESC:Putative amidase Gene:ARO9(YHR137W)|FD-Score:3.87|P-value:5.51E-5||SGD DESC:Aromatic aminotransferase II, catalyzes the first step of tryptophan, phenylalanine, and tyrosine catabolism Gene:ARR2(YPR200C)|FD-Score:-3.2|P-value:6.80E-4||SGD DESC:Arsenate reductase required for arsenate resistance; converts arsenate to arsenite which can then be exported from cells by Arr3p Gene:ATP12(YJL180C)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Conserved protein required for assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase; mutation of human ATP12 reduces active ATP synthase levels and is associated with the disorder ATPAF2 deficiency Gene:BIM1(YER016W)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally Gene:CBC2(YPL178W)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Small subunit of the heterodimeric cap binding complex that also contains Sto1p, component of the spliceosomal commitment complex; interacts with Npl3p, possibly to package mRNA for export from the nucleus; contains an RNA-binding motif Gene:CLB2(YPR119W)|FD-Score:3.92|P-value:4.50E-5||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:CUE1(YMR264W)|FD-Score:5.64|P-value:8.64E-9||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:DYN1(YKR054C)|FD-Score:-3.82|P-value:6.66E-5||SGD DESC:Cytoplasmic heavy chain dynein, microtubule motor protein, required for anaphase spindle elongation; involved in spindle assembly, chromosome movement, and spindle orientation during cell division, targeted to microtubule tips by Pac1p Gene:ECM30(YLR436C)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants have abormal relative levels of mannose and glucose and have Gap1p sorting and transport defects; (GFP)-fusion protein localizes to the cytoplasm Gene:FMP23(YBR047W_p)|FD-Score:3.22|P-value:6.49E-4||SGD DESC:Putative protein of unknown function; proposed to be involved in iron or copper homeostasis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FPR4(YLR449W)|FD-Score:3.91|P-value:4.69E-5||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase) (proline isomerase) localized to the nucleus; catalyzes isomerization of proline residues in histones H3 and H4, which affects lysine methylation of those histones Gene:GCN3(YKR026C)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCS1(YDL226C)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GSP2(YOR185C)|FD-Score:3.76|P-value:8.42E-5||SGD DESC:GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability; protein abundance increases in response to DNA replication stress Gene:HAP2(YGL237C)|FD-Score:3.91|P-value:4.58E-5||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding Gene:HNT3(YOR258W)|FD-Score:4.28|P-value:9.43E-6||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:HST1(YOL068C)|FD-Score:-3.16|P-value:7.97E-4||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:HUR1(YGL168W)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene Gene:HXK1(YFR053C)|FD-Score:-3.33|P-value:4.42E-4||SGD DESC:Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication Gene:IRE1(YHR079C)|FD-Score:4.53|P-value:2.99E-6||SGD DESC:Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress Gene:LEM3(YNL323W)|FD-Score:-4.41|P-value:5.28E-6||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:MEH1(YKR007W)|FD-Score:-3.29|P-value:4.96E-4||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification Gene:MGR2(YPL098C)|FD-Score:-3.19|P-value:7.20E-4||SGD DESC:Subunit of the TIM23 translocase complex; acts to couple Tim21p with the core Tim23 translocase; absolutely required for mitochondrial import of presequence-containing proteins at elevated temperature; required for viability of cells lacking the mitochondrial genome (petite-negative phenotype) Gene:MRPL40(YPL173W)|FD-Score:3.85|P-value:5.82E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSA1(YOR066W)|FD-Score:-3.85|P-value:5.88E-5||SGD DESC:Activator of G1-specific transcription factors, MBF and SBF; involved in regulation of the timing of G1-specific gene transcription and cell cycle initiation; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:MSI1(YBR195C)|FD-Score:3.82|P-value:6.70E-5||SGD DESC:Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription; Msi1p localizes to both nucleus and cytoplasm and has an independent role as a negative regulator of the RAS/cAMP pathway via sequestration of Npr1p kinase Gene:NCL1(YBL024W)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120 Gene:NMD2(YHR077C)|FD-Score:-4.62|P-value:1.91E-6||SGD DESC:Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance Gene:NUP60(YAR002W)|FD-Score:4.09|P-value:2.20E-5||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; both NUP1 and NUP60 are homologous to human NUP153 Gene:PAC10(YGR078C)|FD-Score:4.31|P-value:8.22E-6||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PAP2(YOL115W)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Trf5p Gene:PIN4(YBL051C)|FD-Score:-3.23|P-value:6.29E-4||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:PIR3(YKL163W)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:O-glycosylated covalently-bound cell wall protein; required for cell wall stability; expression is cell cycle regulated, peaking in M/G1 and also subject to regulation by the cell integrity pathway; PIR3 has a paralog, HSP150, that arose from the whole genome duplication Gene:PUS4(YNL292W)|FD-Score:4.96|P-value:3.52E-7||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine-55 (Psi55), a highly conserved tRNA modification, in mitochondrial and cytoplasmic tRNAs; PUS4 overexpression leads to translational derepression of GCN4 (Gcd- phenotype) Gene:REF2(YDR195W)|FD-Score:3.11|P-value:9.44E-4||SGD DESC:RNA-binding protein involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes Gene:RIF1(YBR275C)|FD-Score:4.55|P-value:2.72E-6||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RIM9(YMR063W)|FD-Score:3.1|P-value:9.60E-4||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RNH1(YMR234W)|FD-Score:-4.95|P-value:3.71E-7||SGD DESC:Ribonuclease H1; able to bind double-stranded RNAs and RNA-DNA hybrids; associates with RNAse polymerase I; the homolog of mammalian RNAse HII (the S. cerevisiae homolog of mammalian RNAse HI is RNH201) Gene:ROM2(YLR371W)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL35A(YDL191W)|FD-Score:-3.27|P-value:5.45E-4||SGD DESC:Ribosomal 60S subunit protein L35A; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35A has a paralog, RPL35B, that arose from the whole genome duplication Gene:RRI2(YOL117W)|FD-Score:3.29|P-value:5.05E-4||SGD DESC:Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response Gene:RRT13(YER066W_p)|FD-Score:3.13|P-value:8.89E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with decreased levels of rDNA transcription Gene:RSR1(YGR152C)|FD-Score:5.84|P-value:2.63E-9||SGD DESC:GTP-binding protein of the ras superfamily required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases Gene:SAM37(YMR060C)|FD-Score:3.12|P-value:8.93E-4||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SBH2(YER019C-A)|FD-Score:-3.11|P-value:9.25E-4||SGD DESC:Ssh1p-Sss1p-Sbh2p complex component, involved in protein translocation into the endoplasmic reticulum; homologous to Sbh1p Gene:SBP1(YHL034C)|FD-Score:6.21|P-value:2.58E-10||SGD DESC:Protein that binds eIF4G and has a role in repression of translation; has an RGG motif; found in cytoplasmic P bodies; found associated with small nucleolar RNAs snR10 and snR11 Gene:SEG1(YMR086W)|FD-Score:4.82|P-value:7.25E-7||SGD DESC:Component of eisosome required for proper eisosome assembly; precedes Pil1p/Lsp1p during eisosome formation and controls eisosome length and shape; Seg1p-GFP diffusely distributed and forms heterogeneous patches at the plasma membrane in small buds, also found in medium and large buds; may interact with ribosomes, based on co-purification experiments; expression is repressed by cAMP; similar to A. gossypii SEG gene and to S. pombe Sle1p, important for generating eisosomes Gene:SOH1(YGL127C)|FD-Score:3.73|P-value:9.42E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits Gene:SPH1(YLR313C)|FD-Score:3.25|P-value:5.87E-4||SGD DESC:Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p Gene:SPO14(YKR031C)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Phospholipase D; catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions Gene:TCB1(YOR086C)|FD-Score:-4.16|P-value:1.60E-5||SGD DESC:Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact Gene:TRP1(YDR007W)|FD-Score:6.52|P-value:3.43E-11||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:3.12|P-value:9.11E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:3.73|P-value:9.61E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRS33(YOR115C)|FD-Score:-4.09|P-value:2.16E-5||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:UPC2(YDR213W)|FD-Score:-3.12|P-value:8.94E-4||SGD DESC:Sterol regulatory element binding protein; induces transcription of sterol biosynthetic genes and of DAN/TIR gene products; relocates from intracellular membranes to perinuclear foci on sterol depletion; UPC2 has a paralog, ECM22, that arose from the whole genome duplication Gene:URC2(YDR520C_p)|FD-Score:3.22|P-value:6.36E-4||SGD DESC:Putative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism Gene:VMA3(YEL027W)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:Proteolipid subunit of the vacuolar H(+)-ATPase V0 sector (subunit c; dicyclohexylcarbodiimide binding subunit); required for vacuolar acidification and important for copper and iron metal ion homeostasis Gene:VMA4(YOR332W)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress Gene:XRS2(YDR369C)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling Gene:YBP2(YGL060W)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Central kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication Gene:YBR053C(YBR053C_p)|FD-Score:3.86|P-value:5.59E-5||SGD DESC:Putative protein of unknown function; induced by cell wall perturbation Gene:YDL119C(YDL119C_p)|FD-Score:-3.14|P-value:8.52E-4||SGD DESC:Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria Gene:YDR248C(YDR248C_p)|FD-Score:5.49|P-value:1.97E-8||SGD DESC:Putative gluconokinase; sequence similarity to bacterial and human gluconokinase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; upregulated by deletion of the RNAP-II associated factor, PAF1 Gene:YDR445C(YDR445C_d)|FD-Score:3.23|P-value:6.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR467C(YDR467C_d)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFL019C(YFL019C_d)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene Gene:YFR045W(YFR045W_p)|FD-Score:5.08|P-value:1.92E-7||SGD DESC:Putative mitochondrial transport protein; null mutant is viable, exhibits decreased levels of chitin and normal resistance to calcofluor white Gene:YGL132W(YGL132W_d)|FD-Score:-3.39|P-value:3.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SNT2/YGL131C Gene:YIL060W(YIL060W_p)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene Gene:YIR035C(YIR035C_p)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Putative cytoplasmic short-chain dehydrogenase/reductase Gene:YJL070C(YJL070C_p)|FD-Score:-3.23|P-value:6.11E-4||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; may regulate purine nucleotide homeostasis as overexpression in an AMD1 strain grown in adenine results in greatly reduced GDP and GTP intracellular levels; not an essential gene; YJL070C has a paralog, YBR284W, that arose from the whole genome duplication Gene:YMR310C(YMR310C_p)|FD-Score:3.6|P-value:1.56E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR310C is not an essential gene Gene:YNG1(YOR064C)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3, has similarity to the human tumor suppressor ING1 Gene:YNL010W(YNL010W_p)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Putative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation Gene:YNL097C-B(YNL097C-B_p)|FD-Score:3.43|P-value:3.07E-4||SGD DESC:Putative protein of unknown function Gene:YNR065C(YNR065C_p)|FD-Score:5|P-value:2.94E-7||SGD DESC:Protein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene Gene:YOL153C(YOL153C)|FD-Score:3.28|P-value:5.20E-4||SGD DESC:Hypothetical protein Gene:YPL073C(YPL073C_d)|FD-Score:3.85|P-value:5.99E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with verified gene UBP16/YPL072W; may interact with ribosome based on co-purification experiments Gene:YTP1(YNL237W)|FD-Score:-3.87|P-value:5.41E-5||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins Gene:ABZ1(YNR033W)|FD-Score:3.31|P-value:4.67E-4||SGD DESC:Para-aminobenzoate (PABA) synthase; has similarity to Escherichia coli PABA synthase components PabA and PabB; required for the synthesis of para-aminobenzoic acid, an important intermediate for folate and ubiquinone Q biosynthesis; protein abundance increases in response to DNA replication stress Gene:ADO1(YJR105W)|FD-Score:-4.11|P-value:1.95E-5||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:AIF1(YNR074C)|FD-Score:-3.39|P-value:3.54E-4||SGD DESC:Mitochondrial cell death effector that translocates to the nucleus in response to apoptotic stimuli, homolog of mammalian Apoptosis-Inducing Factor, putative reductase Gene:ALR2(YFL050C)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Probable Mg(2+) transporter; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions; plays a role in regulating Ty1 transposition Gene:AMD2(YDR242W)|FD-Score:3.8|P-value:7.18E-5||SGD DESC:Putative amidase Gene:ARO9(YHR137W)|FD-Score:3.87|P-value:5.51E-5||SGD DESC:Aromatic aminotransferase II, catalyzes the first step of tryptophan, phenylalanine, and tyrosine catabolism Gene:ARR2(YPR200C)|FD-Score:-3.2|P-value:6.80E-4||SGD DESC:Arsenate reductase required for arsenate resistance; converts arsenate to arsenite which can then be exported from cells by Arr3p Gene:ATP12(YJL180C)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Conserved protein required for assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase; mutation of human ATP12 reduces active ATP synthase levels and is associated with the disorder ATPAF2 deficiency Gene:BIM1(YER016W)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally Gene:CBC2(YPL178W)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Small subunit of the heterodimeric cap binding complex that also contains Sto1p, component of the spliceosomal commitment complex; interacts with Npl3p, possibly to package mRNA for export from the nucleus; contains an RNA-binding motif Gene:CLB2(YPR119W)|FD-Score:3.92|P-value:4.50E-5||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:CUE1(YMR264W)|FD-Score:5.64|P-value:8.64E-9||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:DYN1(YKR054C)|FD-Score:-3.82|P-value:6.66E-5||SGD DESC:Cytoplasmic heavy chain dynein, microtubule motor protein, required for anaphase spindle elongation; involved in spindle assembly, chromosome movement, and spindle orientation during cell division, targeted to microtubule tips by Pac1p Gene:ECM30(YLR436C)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants have abormal relative levels of mannose and glucose and have Gap1p sorting and transport defects; (GFP)-fusion protein localizes to the cytoplasm Gene:FMP23(YBR047W_p)|FD-Score:3.22|P-value:6.49E-4||SGD DESC:Putative protein of unknown function; proposed to be involved in iron or copper homeostasis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FPR4(YLR449W)|FD-Score:3.91|P-value:4.69E-5||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase) (proline isomerase) localized to the nucleus; catalyzes isomerization of proline residues in histones H3 and H4, which affects lysine methylation of those histones Gene:GCN3(YKR026C)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCS1(YDL226C)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GSP2(YOR185C)|FD-Score:3.76|P-value:8.42E-5||SGD DESC:GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability; protein abundance increases in response to DNA replication stress Gene:HAP2(YGL237C)|FD-Score:3.91|P-value:4.58E-5||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding Gene:HNT3(YOR258W)|FD-Score:4.28|P-value:9.43E-6||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:HST1(YOL068C)|FD-Score:-3.16|P-value:7.97E-4||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:HUR1(YGL168W)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene Gene:HXK1(YFR053C)|FD-Score:-3.33|P-value:4.42E-4||SGD DESC:Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication Gene:IRE1(YHR079C)|FD-Score:4.53|P-value:2.99E-6||SGD DESC:Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress Gene:LEM3(YNL323W)|FD-Score:-4.41|P-value:5.28E-6||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:MEH1(YKR007W)|FD-Score:-3.29|P-value:4.96E-4||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification Gene:MGR2(YPL098C)|FD-Score:-3.19|P-value:7.20E-4||SGD DESC:Subunit of the TIM23 translocase complex; acts to couple Tim21p with the core Tim23 translocase; absolutely required for mitochondrial import of presequence-containing proteins at elevated temperature; required for viability of cells lacking the mitochondrial genome (petite-negative phenotype) Gene:MRPL40(YPL173W)|FD-Score:3.85|P-value:5.82E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSA1(YOR066W)|FD-Score:-3.85|P-value:5.88E-5||SGD DESC:Activator of G1-specific transcription factors, MBF and SBF; involved in regulation of the timing of G1-specific gene transcription and cell cycle initiation; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:MSI1(YBR195C)|FD-Score:3.82|P-value:6.70E-5||SGD DESC:Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription; Msi1p localizes to both nucleus and cytoplasm and has an independent role as a negative regulator of the RAS/cAMP pathway via sequestration of Npr1p kinase Gene:NCL1(YBL024W)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120 Gene:NMD2(YHR077C)|FD-Score:-4.62|P-value:1.91E-6||SGD DESC:Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance Gene:NUP60(YAR002W)|FD-Score:4.09|P-value:2.20E-5||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; both NUP1 and NUP60 are homologous to human NUP153 Gene:PAC10(YGR078C)|FD-Score:4.31|P-value:8.22E-6||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PAP2(YOL115W)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Trf5p Gene:PIN4(YBL051C)|FD-Score:-3.23|P-value:6.29E-4||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:PIR3(YKL163W)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:O-glycosylated covalently-bound cell wall protein; required for cell wall stability; expression is cell cycle regulated, peaking in M/G1 and also subject to regulation by the cell integrity pathway; PIR3 has a paralog, HSP150, that arose from the whole genome duplication Gene:PUS4(YNL292W)|FD-Score:4.96|P-value:3.52E-7||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine-55 (Psi55), a highly conserved tRNA modification, in mitochondrial and cytoplasmic tRNAs; PUS4 overexpression leads to translational derepression of GCN4 (Gcd- phenotype) Gene:REF2(YDR195W)|FD-Score:3.11|P-value:9.44E-4||SGD DESC:RNA-binding protein involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes Gene:RIF1(YBR275C)|FD-Score:4.55|P-value:2.72E-6||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RIM9(YMR063W)|FD-Score:3.1|P-value:9.60E-4||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RNH1(YMR234W)|FD-Score:-4.95|P-value:3.71E-7||SGD DESC:Ribonuclease H1; able to bind double-stranded RNAs and RNA-DNA hybrids; associates with RNAse polymerase I; the homolog of mammalian RNAse HII (the S. cerevisiae homolog of mammalian RNAse HI is RNH201) Gene:ROM2(YLR371W)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL35A(YDL191W)|FD-Score:-3.27|P-value:5.45E-4||SGD DESC:Ribosomal 60S subunit protein L35A; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35A has a paralog, RPL35B, that arose from the whole genome duplication Gene:RRI2(YOL117W)|FD-Score:3.29|P-value:5.05E-4||SGD DESC:Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response Gene:RRT13(YER066W_p)|FD-Score:3.13|P-value:8.89E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with decreased levels of rDNA transcription Gene:RSR1(YGR152C)|FD-Score:5.84|P-value:2.63E-9||SGD DESC:GTP-binding protein of the ras superfamily required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases Gene:SAM37(YMR060C)|FD-Score:3.12|P-value:8.93E-4||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SBH2(YER019C-A)|FD-Score:-3.11|P-value:9.25E-4||SGD DESC:Ssh1p-Sss1p-Sbh2p complex component, involved in protein translocation into the endoplasmic reticulum; homologous to Sbh1p Gene:SBP1(YHL034C)|FD-Score:6.21|P-value:2.58E-10||SGD DESC:Protein that binds eIF4G and has a role in repression of translation; has an RGG motif; found in cytoplasmic P bodies; found associated with small nucleolar RNAs snR10 and snR11 Gene:SEG1(YMR086W)|FD-Score:4.82|P-value:7.25E-7||SGD DESC:Component of eisosome required for proper eisosome assembly; precedes Pil1p/Lsp1p during eisosome formation and controls eisosome length and shape; Seg1p-GFP diffusely distributed and forms heterogeneous patches at the plasma membrane in small buds, also found in medium and large buds; may interact with ribosomes, based on co-purification experiments; expression is repressed by cAMP; similar to A. gossypii SEG gene and to S. pombe Sle1p, important for generating eisosomes Gene:SOH1(YGL127C)|FD-Score:3.73|P-value:9.42E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits Gene:SPH1(YLR313C)|FD-Score:3.25|P-value:5.87E-4||SGD DESC:Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p Gene:SPO14(YKR031C)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Phospholipase D; catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions Gene:TCB1(YOR086C)|FD-Score:-4.16|P-value:1.60E-5||SGD DESC:Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact Gene:TRP1(YDR007W)|FD-Score:6.52|P-value:3.43E-11||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:3.12|P-value:9.11E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:3.73|P-value:9.61E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRS33(YOR115C)|FD-Score:-4.09|P-value:2.16E-5||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:UPC2(YDR213W)|FD-Score:-3.12|P-value:8.94E-4||SGD DESC:Sterol regulatory element binding protein; induces transcription of sterol biosynthetic genes and of DAN/TIR gene products; relocates from intracellular membranes to perinuclear foci on sterol depletion; UPC2 has a paralog, ECM22, that arose from the whole genome duplication Gene:URC2(YDR520C_p)|FD-Score:3.22|P-value:6.36E-4||SGD DESC:Putative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism Gene:VMA3(YEL027W)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:Proteolipid subunit of the vacuolar H(+)-ATPase V0 sector (subunit c; dicyclohexylcarbodiimide binding subunit); required for vacuolar acidification and important for copper and iron metal ion homeostasis Gene:VMA4(YOR332W)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress Gene:XRS2(YDR369C)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling Gene:YBP2(YGL060W)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Central kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication Gene:YBR053C(YBR053C_p)|FD-Score:3.86|P-value:5.59E-5||SGD DESC:Putative protein of unknown function; induced by cell wall perturbation Gene:YDL119C(YDL119C_p)|FD-Score:-3.14|P-value:8.52E-4||SGD DESC:Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria Gene:YDR248C(YDR248C_p)|FD-Score:5.49|P-value:1.97E-8||SGD DESC:Putative gluconokinase; sequence similarity to bacterial and human gluconokinase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; upregulated by deletion of the RNAP-II associated factor, PAF1 Gene:YDR445C(YDR445C_d)|FD-Score:3.23|P-value:6.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR467C(YDR467C_d)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFL019C(YFL019C_d)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene Gene:YFR045W(YFR045W_p)|FD-Score:5.08|P-value:1.92E-7||SGD DESC:Putative mitochondrial transport protein; null mutant is viable, exhibits decreased levels of chitin and normal resistance to calcofluor white Gene:YGL132W(YGL132W_d)|FD-Score:-3.39|P-value:3.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SNT2/YGL131C Gene:YIL060W(YIL060W_p)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene Gene:YIR035C(YIR035C_p)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Putative cytoplasmic short-chain dehydrogenase/reductase Gene:YJL070C(YJL070C_p)|FD-Score:-3.23|P-value:6.11E-4||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; may regulate purine nucleotide homeostasis as overexpression in an AMD1 strain grown in adenine results in greatly reduced GDP and GTP intracellular levels; not an essential gene; YJL070C has a paralog, YBR284W, that arose from the whole genome duplication Gene:YMR310C(YMR310C_p)|FD-Score:3.6|P-value:1.56E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR310C is not an essential gene Gene:YNG1(YOR064C)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3, has similarity to the human tumor suppressor ING1 Gene:YNL010W(YNL010W_p)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Putative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation Gene:YNL097C-B(YNL097C-B_p)|FD-Score:3.43|P-value:3.07E-4||SGD DESC:Putative protein of unknown function Gene:YNR065C(YNR065C_p)|FD-Score:5|P-value:2.94E-7||SGD DESC:Protein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene Gene:YOL153C(YOL153C)|FD-Score:3.28|P-value:5.20E-4||SGD DESC:Hypothetical protein Gene:YPL073C(YPL073C_d)|FD-Score:3.85|P-value:5.99E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with verified gene UBP16/YPL072W; may interact with ribosome based on co-purification experiments Gene:YTP1(YNL237W)|FD-Score:-3.87|P-value:5.41E-5||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR194C5.774.03E-90.51TOA1TFIIA large subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to largest and second largest subunits of human and Drosophila TFIIA
YBL097W5.581.23E-80.51BRN1Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission
YJL014W5.131.44E-70.51CCT3Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YLR163C4.875.67E-70.51MAS1Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins
YNL112W4.671.50E-60.51DBP2ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites
YEL034W4.671.53E-60.51HYP2Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication
YDR145W4.299.07E-60.51TAF12Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A
YOR224C3.787.95E-50.09RPB8RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III
YKL145W3.681.14E-40.06RPT1One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance
YML085C3.621.45E-40.04TUB1Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication
YNL282W3.591.68E-40.02POP3Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YJL010C3.571.78E-40.01NOP9Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain
YPR107C3.561.85E-40.16YTH1Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation
YPR133C3.413.31E-40.00SPN1Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype
YDL193W3.403.33E-40.01NUS1Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR007W6.523.43E-11TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YHL034C6.212.58E-10SBP1Protein that binds eIF4G and has a role in repression of translation; has an RGG motif; found in cytoplasmic P bodies; found associated with small nucleolar RNAs snR10 and snR11
YGR152C5.842.63E-9RSR1GTP-binding protein of the ras superfamily required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases
YMR264W5.648.64E-9CUE1Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication
YDR248C_p5.491.97E-8YDR248C_pPutative gluconokinase; sequence similarity to bacterial and human gluconokinase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; upregulated by deletion of the RNAP-II associated factor, PAF1
YFR045W_p5.081.92E-7YFR045W_pPutative mitochondrial transport protein; null mutant is viable, exhibits decreased levels of chitin and normal resistance to calcofluor white
YNR065C_p5.002.94E-7YNR065C_pProtein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene
YNL292W4.963.52E-7PUS4Pseudouridine synthase, catalyzes only the formation of pseudouridine-55 (Psi55), a highly conserved tRNA modification, in mitochondrial and cytoplasmic tRNAs; PUS4 overexpression leads to translational derepression of GCN4 (Gcd- phenotype)
YMR086W4.827.25E-7SEG1Component of eisosome required for proper eisosome assembly; precedes Pil1p/Lsp1p during eisosome formation and controls eisosome length and shape; Seg1p-GFP diffusely distributed and forms heterogeneous patches at the plasma membrane in small buds, also found in medium and large buds; may interact with ribosomes, based on co-purification experiments; expression is repressed by cAMP; similar to A. gossypii SEG gene and to S. pombe Sle1p, important for generating eisosomes
YBR275C4.552.72E-6RIF1Protein that binds to the Rap1p C-terminus and acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation
YHR079C4.532.99E-6IRE1Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress
YGR078C4.318.22E-6PAC10Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding
YOR258W4.289.43E-6HNT3DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress
YAR002W4.092.20E-5NUP60FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; both NUP1 and NUP60 are homologous to human NUP153
YPR119W3.924.50E-5CLB2B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome

GO enrichment analysis for SGTC_1003
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1256.51E-22SGTC_2733amorolfine 100.0 μMMiscellaneous542600.0615385plasma membrane duress
0.1219.98E-21SGTC_21375261020 140.0 μMChembridge (Fragment library)7636550.1
0.1163.11E-19SGTC_18815652484 16.0 μMMiscellaneous22551290.144928TRP & mitochondrial translation
0.1141.57E-18SGTC_1572epiandrosterone 68.9 μMTimTec (Pure natural product library)4413020.0461538
0.1141.72E-18SGTC_23849036245 200.0 μMChembridge (Fragment library)64627660.0862069
0.1141.91E-18SGTC_2540epiandrosterone 100.0 μMTimTec (Pure natural product library)4413020.0461538
0.1126.07E-18SGTC_20935331342 194.4 μMChembridge (Fragment library)338390.05
0.1111.22E-17SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.0625
0.1111.72E-17SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0246914
0.1088.28E-17SGTC_2735sertaconazole 140.0 nMMiscellaneous658630.05plasma membrane duress
0.1071.24E-16SGTC_10054092-0690 4.4 μMChemDiv (Drug-like library)15293430.0606061plasma membrane duress
0.1054.95E-16SGTC_14354092-0789 56.7 μMChemDiv (Drug-like library)22482850.0645161plasma membrane duress
0.1041.03E-15SGTC_5831488-1091 88.9 μMChemDiv (Drug-like library)43032730.245283
0.1022.98E-15SGTC_14554262-0120 131.0 μMChemDiv (Drug-like library)15295870.102041plasma membrane duress
0.1024.72E-15SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.04

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_14294049-02059.46 μM0.61538522774478ChemDiv (Drug-like library)344.1993433.75714
SGTC_10024049-0206172 μM0.558144662749ChemDiv (Drug-like library)310.3008432.90316
SGTC_340469-068381.75 μM0.53690923ChemDiv (Drug-like library)337.307942.59217SWF1 & branched chain AA biosynthesis
SGTC_3312913333071.43 μM0.47727317617324Chembridge (Drug-like library)307.3830233.87914
SGTC_7580868-002916 μM0.47619762687ChemDiv (Drug-like library)327.3726634.04114
SGTC_5694130-2812160 μM0.428571743552ChemDiv (Drug-like library)279.3298633.01614amide catabolism
SGTC_14324049-029868.4 μM0.422222741646ChemDiv (Drug-like library)299.2931462.71216
SGTC_14284049-020461.7 μM0.416667X1428ChemDiv (Drug-like library)324.327423.38941
SGTC_5414513-132194 μM0.409091747515ChemDiv (Drug-like library)305.391963.51314
SGTC_2934049-027338.5 μM0.3913041380311ChemDiv (Drug-like library)380.180274.00816