4130-3005

benzotriazol-1-yl-(3-chlorophenyl)methanone

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1014
Screen concentration 69.3 μM
Source ChemDiv (Drug-like library)
PubChem CID 743608
SMILES C1=CC=C2C(=C1)N=NN2C(=O)C3=CC(=CC=C3)Cl
Standardized SMILES Clc1cccc(c1)C(=O)n2nnc3ccccc23
Molecular weight 257.6751
ALogP 3.75
H-bond donor count 0
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 7.02
% growth inhibition (Hom. pool) 2.44


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 743608
Download HIP data (tab-delimited text)  (excel)
Gene:CDC25(YLR310C)|FD-Score:3.46|P-value:2.69E-4|Clearance:0.04||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:DBP10(YDL031W)|FD-Score:3.42|P-value:3.13E-4|Clearance:0.26||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box protein family, constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis Gene:DBP6(YNR038W)|FD-Score:4.17|P-value:1.53E-5|Clearance:0.04||SGD DESC:Essential protein involved in ribosome biogenesis; putative ATP-dependent RNA helicase of the DEAD-box protein family Gene:EFB1(YAL003W)|FD-Score:3.92|P-value:4.36E-5|Clearance:0.44||SGD DESC:Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site Gene:HRR25(YPL204W)|FD-Score:-3.78|P-value:7.91E-5|Clearance:0||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:INO80(YGL150C)|FD-Score:3.99|P-value:3.35E-5|Clearance:0.06||SGD DESC:ATPase and nucleosome spacing factor, subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; has a role in modulating stress gene transcription Gene:KRE9(YJL174W)|FD-Score:-4.42|P-value:5.04E-6|Clearance:0||SGD DESC:Glycoprotein involved in cell wall beta-glucan assembly; null mutation leads to severe growth defects, aberrant multibudded morphology, and mating defects Gene:NET1(YJL076W)|FD-Score:4.57|P-value:2.40E-6|Clearance:0.4||SGD DESC:Core subunit of the RENT complex; involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure; NET1 has a paralog, TOF2, that arose from the whole genome duplication Gene:NOP53(YPL146C)|FD-Score:3.16|P-value:7.85E-4|Clearance:0.02||SGD DESC:Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired Gene:PGS1(YCL004W)|FD-Score:6.22|P-value:2.49E-10|Clearance:1.65||SGD DESC:Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis Gene:RPA43(YOR340C)|FD-Score:3.49|P-value:2.44E-4|Clearance:0.03||SGD DESC:RNA polymerase I subunit A43 Gene:RPF2(YKR081C)|FD-Score:3.14|P-value:8.42E-4|Clearance:0.25||SGD DESC:Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles Gene:RPS5(YJR123W)|FD-Score:6.78|P-value:5.98E-12|Clearance:1.65||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:SEC13(YLR208W)|FD-Score:-3.75|P-value:8.82E-5|Clearance:0||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SMT3(YDR510W)|FD-Score:-3.22|P-value:6.34E-4|Clearance:0||SGD DESC:Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2 Gene:YTM1(YOR272W)|FD-Score:4.13|P-value:1.80E-5|Clearance:0.15||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats Gene:CDC25(YLR310C)|FD-Score:3.46|P-value:2.69E-4|Clearance:0.04||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:DBP10(YDL031W)|FD-Score:3.42|P-value:3.13E-4|Clearance:0.26||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box protein family, constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis Gene:DBP6(YNR038W)|FD-Score:4.17|P-value:1.53E-5|Clearance:0.04||SGD DESC:Essential protein involved in ribosome biogenesis; putative ATP-dependent RNA helicase of the DEAD-box protein family Gene:EFB1(YAL003W)|FD-Score:3.92|P-value:4.36E-5|Clearance:0.44||SGD DESC:Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site Gene:HRR25(YPL204W)|FD-Score:-3.78|P-value:7.91E-5|Clearance:0||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:INO80(YGL150C)|FD-Score:3.99|P-value:3.35E-5|Clearance:0.06||SGD DESC:ATPase and nucleosome spacing factor, subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; has a role in modulating stress gene transcription Gene:KRE9(YJL174W)|FD-Score:-4.42|P-value:5.04E-6|Clearance:0||SGD DESC:Glycoprotein involved in cell wall beta-glucan assembly; null mutation leads to severe growth defects, aberrant multibudded morphology, and mating defects Gene:NET1(YJL076W)|FD-Score:4.57|P-value:2.40E-6|Clearance:0.4||SGD DESC:Core subunit of the RENT complex; involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure; NET1 has a paralog, TOF2, that arose from the whole genome duplication Gene:NOP53(YPL146C)|FD-Score:3.16|P-value:7.85E-4|Clearance:0.02||SGD DESC:Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired Gene:PGS1(YCL004W)|FD-Score:6.22|P-value:2.49E-10|Clearance:1.65||SGD DESC:Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis Gene:RPA43(YOR340C)|FD-Score:3.49|P-value:2.44E-4|Clearance:0.03||SGD DESC:RNA polymerase I subunit A43 Gene:RPF2(YKR081C)|FD-Score:3.14|P-value:8.42E-4|Clearance:0.25||SGD DESC:Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles Gene:RPS5(YJR123W)|FD-Score:6.78|P-value:5.98E-12|Clearance:1.65||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:SEC13(YLR208W)|FD-Score:-3.75|P-value:8.82E-5|Clearance:0||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SMT3(YDR510W)|FD-Score:-3.22|P-value:6.34E-4|Clearance:0||SGD DESC:Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2 Gene:YTM1(YOR272W)|FD-Score:4.13|P-value:1.80E-5|Clearance:0.15||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 743608
Download HOP data (tab-delimited text)  (excel)
Gene:ADH6(YMR318C)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance; protein abundance increases in response to DNA replication stress Gene:ADK1(YDR226W)|FD-Score:3.87|P-value:5.50E-5||SGD DESC:Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress Gene:APS1(YLR170C)|FD-Score:-4.01|P-value:3.08E-5||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex Gene:ATG21(YPL100W)|FD-Score:3.28|P-value:5.15E-4||SGD DESC:Phosphoinositide binding protein required for vesicle formation in the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein Gene:ATP2(YJR121W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:BIM1(YER016W)|FD-Score:3.13|P-value:8.66E-4||SGD DESC:Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally Gene:BIT2(YBR270C_p)|FD-Score:3.74|P-value:9.06E-5||SGD DESC:Subunit of TORC2 membrane-associated complex; involved in regulation of actin cytoskeletal dynamics during polarized growth and cell wall integrity; interacts with Slm1p and Slm2p, homologous PH domain-containing TORC2 substrates; BIT2 has a paralog, BIT61, that arose from the whole genome duplication Gene:BSP1(YPR171W)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton Gene:BUB3(YOR026W)|FD-Score:3.38|P-value:3.56E-4||SGD DESC:Kinetochore checkpoint WD40 repeat protein that localizes to kinetochores during prophase and metaphase, delays anaphase in the presence of unattached kinetochores; forms complexes with Mad1p-Bub1p and with Cdc20p, binds Mad2p and Mad3p Gene:BUD16(YEL029C)|FD-Score:-3.53|P-value:2.04E-4||SGD DESC:Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) Gene:CDA1(YLR307W)|FD-Score:4.04|P-value:2.68E-5||SGD DESC:Chitin deacetylase, together with Cda2p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall Gene:CDC26(YFR036W)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:COS12(YGL263W)|FD-Score:3.18|P-value:7.25E-4||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:COX18(YGR062C)|FD-Score:3.32|P-value:4.46E-4||SGD DESC:Mitochondrial integral inner membrane protein required for membrane insertion of C-terminus of Cox2p; interacts genetically and physically with Mss2p and Pnt1p; similar to S. cerevisiae Oxa1, N. crassa Oxa2p, and E. coli YidC Gene:CPR7(YJR032W)|FD-Score:5.28|P-value:6.57E-8||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity Gene:CTK3(YML112W)|FD-Score:-3.58|P-value:1.74E-4||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:CUE1(YMR264W)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:DDR2(YOL052C-A)|FD-Score:-3.78|P-value:7.69E-5||SGD DESC:Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication Gene:DLD2(YDL178W)|FD-Score:-3.31|P-value:4.62E-4||SGD DESC:D-lactate dehydrogenase, located in the mitochondrial matrix Gene:DTR1(YBR180W)|FD-Score:4.69|P-value:1.39E-6||SGD DESC:Putative dityrosine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for spore wall synthesis; expressed during sporulation Gene:EXO5(YBR163W)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Mitochondrial 5'-3' exonuclease and sliding exonuclease, required for mitochondrial genome maintenance; distantly related to the RecB nuclease domain of bacterial RecBCD recombinases; may be regulated by the transcription factor Ace2 Gene:FAU1(YER183C)|FD-Score:-3.21|P-value:6.67E-4||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:FMP33(YJL161W_p)|FD-Score:3.19|P-value:7.18E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRK1(YPL141C)|FD-Score:-3.39|P-value:3.51E-4||SGD DESC:Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; interacts with rRNA transcription and ribosome biogenesis factors and the long chain fatty acyl-CoA synthetase Faa3p; FRK1 has a paralog, KIN4, that arose from the whole genome duplication Gene:GUP1(YGL084C)|FD-Score:3.88|P-value:5.19E-5||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HAP4(YKL109W)|FD-Score:-3.65|P-value:1.31E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex Gene:HEH2(YDR458C)|FD-Score:3.1|P-value:9.75E-4||SGD DESC:Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle Gene:HEL1(YKR017C_p)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU) Gene:IMO32(YGR031W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Conserved mitochondrial protein of unknown function; processed by both mitochondrial processing peptidase and mitochondrial octapeptidyl aminopeptidase; gene contains the nested antisense gene NAG1 Gene:KCS1(YDR017C)|FD-Score:4.04|P-value:2.63E-5||SGD DESC:Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; generation of high energy inositol pyrophosphates by Kcs1p is required for many processes such as vacuolar biogenesis, stress response and telomere maintenance Gene:LHS1(YKL073W)|FD-Score:4.18|P-value:1.45E-5||SGD DESC:Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway Gene:MET12(YPL023C)|FD-Score:-5.46|P-value:2.41E-8||SGD DESC:Protein with methylenetetrahydrofolate reductase (MTHFR) activity in vitro; null mutant has no phenotype and is prototrophic for methionine; MET13 encodes major isozyme of MTHFR Gene:MRPL11(YDL202W)|FD-Score:3.28|P-value:5.19E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL16(YBL038W)|FD-Score:4.04|P-value:2.71E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL51(YPR100W)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NDT80(YHR124W)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Meiosis-specific transcription factor required for exit from pachytene and for full meiotic recombination; activates middle sporulation genes; competes with Sum1p for binding to promoters containing middle sporulation elements (MSE) Gene:OM14(YBR230C)|FD-Score:3.15|P-value:8.28E-4||SGD DESC:Integral mitochondrial outer membrane protein; abundance is decreased in cells grown in glucose relative to other carbon sources; appears to contain 3 alpha-helical transmembrane segments; ORF encodes a 97-basepair intron Gene:OPT1(YJL212C)|FD-Score:3.8|P-value:7.33E-5||SGD DESC:Proton-coupled oligopeptide transporter of the plasma membrane; also transports glutathione and phytochelatin; member of the OPT family Gene:PCP1(YGR101W)|FD-Score:-4.66|P-value:1.60E-6||SGD DESC:Mitochondrial serine protease required for the processing of various mitochondrial proteins and maintenance of mitochondrial DNA and morphology; belongs to the rhomboid-GlpG superfamily of intramembrane peptidases Gene:PDR18(YNR070W_p)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:Putative transporter of the ATP-binding cassette (ABC) family, implicated in pleiotropic drug resistance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PEX32(YBR168W)|FD-Score:-3.91|P-value:4.70E-5||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PFK2(YMR205C)|FD-Score:3.72|P-value:9.90E-5||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PPR1(YLR014C)|FD-Score:3.24|P-value:6.03E-4||SGD DESC:Zinc finger transcription factor containing a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of URA1, URA3, URA4, and URA10, which are involved in de novo pyrimidine biosynthesis, in response to pyrimidine starvation; activity may be modulated by interaction with Tup1p Gene:PSY3(YLR376C)|FD-Score:4.22|P-value:1.20E-5||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; deletion of PSY3 results in a mutator phenotype; deletion increases sensitivity to anticancer drugs oxaliplatin and cisplatin but not mitomycin C Gene:PTC7(YHR076W)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:PTH2(YBL057C)|FD-Score:4.88|P-value:5.25E-7||SGD DESC:One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth Gene:RAD55(YDR076W)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p Gene:RGD1(YBR260C)|FD-Score:-3.43|P-value:3.01E-4||SGD DESC:GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization Gene:RNH201(YNL072W)|FD-Score:4.15|P-value:1.63E-5||SGD DESC:Ribonuclease H2 catalytic subunit, removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome Gene:RPL35B(YDL136W)|FD-Score:-3.14|P-value:8.51E-4||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RPP2A(YOL039W)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm Gene:RTS1(YOR014W)|FD-Score:4.09|P-value:2.20E-5||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:SLH1(YGR271W)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses Gene:SPF1(YEL031W)|FD-Score:-3.44|P-value:2.92E-4||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SPT21(YMR179W)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SRL2(YLR082C)|FD-Score:3.21|P-value:6.60E-4||SGD DESC:Protein of unknown function; overexpression suppresses the lethality caused by a rad53 null mutation Gene:TAN1(YGL232W)|FD-Score:-3.2|P-value:6.99E-4||SGD DESC:Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress Gene:TAT1(YBR069C)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TDA10(YGR205W)|FD-Score:-3.21|P-value:6.63E-4||SGD DESC:ATP-binding protein of unknown function; crystal structure resembles that of E.coli pantothenate kinase and other small kinases; null mutant is sensitive to expression of the top1-T722A allele Gene:TGL3(YMR313C)|FD-Score:3.1|P-value:9.52E-4||SGD DESC:Bifunctional enzyme with triacylglycerol lipase and lysophosphatidylethanolamine acyltransferase activity; responsible for all the triacylglycerol lipase activity of the lipid particle; required with Tgl4p for timely bud formation Gene:TOK1(YJL093C)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Outward-rectifier potassium channel of the plasma membrane with two pore domains in tandem, each of which forms a functional channel permeable to potassium; carboxy tail functions to prevent inner gate closures; target of K1 toxin Gene:TRM7(YBR061C)|FD-Score:-3.28|P-value:5.28E-4||SGD DESC:2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively Gene:TSR2(YLR435W)|FD-Score:4.23|P-value:1.16E-5||SGD DESC:Protein with a potential role in pre-rRNA processing Gene:TUB3(YML124C)|FD-Score:5.03|P-value:2.40E-7||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:VAM3(YOR106W)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region Gene:VBA3(YCL069W)|FD-Score:5.21|P-value:9.35E-8||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:WHI3(YNL197C)|FD-Score:3.21|P-value:6.75E-4||SGD DESC:RNA binding protein that sequesters CLN3 mRNA in cytoplasmic foci;; Tpk1 (PKA) mediated phosphorylation of Whi3 affects G1/S transition by modulating Cln3 transcription; cytoplasmic retention factor for Cdc28p and associated cyclins; regulates cell fate and dose-dependently regulates the critical cell size required for passage through Start; WHI3 has a paralog, WHI4, that arose from the whole genome duplication Gene:YAP6(YDR259C)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; overexpression increases sodium and lithium tolerance; computational analysis suggests a role in regulation of expression of genes involved in carbohydrate metabolism; YAP6 has a paralog, CIN5, that arose from the whole genome duplication Gene:YBL100W-C(YBL100W-C_p)|FD-Score:5.49|P-value:2.00E-8||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR277C(YBR277C_d)|FD-Score:3.22|P-value:6.48E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W Gene:YER010C(YER010C)|FD-Score:-3.64|P-value:1.35E-4||SGD DESC:Protein of unknown function, forms a ring-shaped homotrimer; has similarity to members of the prokaryotic rraA family; possibly involved in a phosphotransfer reaction Gene:YFR006W(YFR006W_p)|FD-Score:-3.18|P-value:7.26E-4||SGD DESC:Putative X-Pro aminopeptidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR006W is not an essential gene Gene:YGK3(YOL128C)|FD-Score:4.3|P-value:8.41E-6||SGD DESC:Protein kinase related to mammalian glycogen synthase kinases of the GSK-3 family; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation Gene:YGL007C-A(YGL007C-A_p)|FD-Score:-3.22|P-value:6.43E-4||SGD DESC:Putative protein of unknown function, identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; deletion exhibits slow-growth phenotype; computationally predicted to have a role in cell budding Gene:YGL260W(YGL260W_p)|FD-Score:-4.3|P-value:8.70E-6||SGD DESC:Putative protein of unknown function; transcription is significantly increased in a NAP1 deletion background; deletion mutant has increased accumulation of nickel and selenium Gene:YGR228W(YGR228W_d)|FD-Score:5.99|P-value:1.06E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YJL120W(YJL120W_d)|FD-Score:6.02|P-value:8.94E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YJL150W(YJL150W_d)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL027W(YKL027W)|FD-Score:3.16|P-value:7.80E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YKL115C(YKL115C_d)|FD-Score:4.02|P-value:2.90E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1 Gene:YLR012C(YLR012C_p)|FD-Score:-4.39|P-value:5.72E-6||SGD DESC:Putative protein of unknown function; YLR012C is not an essential gene Gene:YMD8(YML038C)|FD-Score:-3.52|P-value:2.18E-4||SGD DESC:Putative nucleotide sugar transporter, has similarity to Vrg4p Gene:YMR052C-A(YMR052C-A_d)|FD-Score:4.06|P-value:2.51E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR304C-A(YOR304C-A_p)|FD-Score:-5.41|P-value:3.12E-8||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck Gene:YOR325W(YOR325W_d)|FD-Score:3.19|P-value:7.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF FRT1 Gene:YPR084W(YPR084W_p)|FD-Score:3.87|P-value:5.47E-5||SGD DESC:Putative protein of unknown function Gene:ADH6(YMR318C)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance; protein abundance increases in response to DNA replication stress Gene:ADK1(YDR226W)|FD-Score:3.87|P-value:5.50E-5||SGD DESC:Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress Gene:APS1(YLR170C)|FD-Score:-4.01|P-value:3.08E-5||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex Gene:ATG21(YPL100W)|FD-Score:3.28|P-value:5.15E-4||SGD DESC:Phosphoinositide binding protein required for vesicle formation in the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein Gene:ATP2(YJR121W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:BIM1(YER016W)|FD-Score:3.13|P-value:8.66E-4||SGD DESC:Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally Gene:BIT2(YBR270C_p)|FD-Score:3.74|P-value:9.06E-5||SGD DESC:Subunit of TORC2 membrane-associated complex; involved in regulation of actin cytoskeletal dynamics during polarized growth and cell wall integrity; interacts with Slm1p and Slm2p, homologous PH domain-containing TORC2 substrates; BIT2 has a paralog, BIT61, that arose from the whole genome duplication Gene:BSP1(YPR171W)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton Gene:BUB3(YOR026W)|FD-Score:3.38|P-value:3.56E-4||SGD DESC:Kinetochore checkpoint WD40 repeat protein that localizes to kinetochores during prophase and metaphase, delays anaphase in the presence of unattached kinetochores; forms complexes with Mad1p-Bub1p and with Cdc20p, binds Mad2p and Mad3p Gene:BUD16(YEL029C)|FD-Score:-3.53|P-value:2.04E-4||SGD DESC:Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) Gene:CDA1(YLR307W)|FD-Score:4.04|P-value:2.68E-5||SGD DESC:Chitin deacetylase, together with Cda2p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall Gene:CDC26(YFR036W)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:COS12(YGL263W)|FD-Score:3.18|P-value:7.25E-4||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:COX18(YGR062C)|FD-Score:3.32|P-value:4.46E-4||SGD DESC:Mitochondrial integral inner membrane protein required for membrane insertion of C-terminus of Cox2p; interacts genetically and physically with Mss2p and Pnt1p; similar to S. cerevisiae Oxa1, N. crassa Oxa2p, and E. coli YidC Gene:CPR7(YJR032W)|FD-Score:5.28|P-value:6.57E-8||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity Gene:CTK3(YML112W)|FD-Score:-3.58|P-value:1.74E-4||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:CUE1(YMR264W)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:DDR2(YOL052C-A)|FD-Score:-3.78|P-value:7.69E-5||SGD DESC:Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication Gene:DLD2(YDL178W)|FD-Score:-3.31|P-value:4.62E-4||SGD DESC:D-lactate dehydrogenase, located in the mitochondrial matrix Gene:DTR1(YBR180W)|FD-Score:4.69|P-value:1.39E-6||SGD DESC:Putative dityrosine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for spore wall synthesis; expressed during sporulation Gene:EXO5(YBR163W)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Mitochondrial 5'-3' exonuclease and sliding exonuclease, required for mitochondrial genome maintenance; distantly related to the RecB nuclease domain of bacterial RecBCD recombinases; may be regulated by the transcription factor Ace2 Gene:FAU1(YER183C)|FD-Score:-3.21|P-value:6.67E-4||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:FMP33(YJL161W_p)|FD-Score:3.19|P-value:7.18E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRK1(YPL141C)|FD-Score:-3.39|P-value:3.51E-4||SGD DESC:Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; interacts with rRNA transcription and ribosome biogenesis factors and the long chain fatty acyl-CoA synthetase Faa3p; FRK1 has a paralog, KIN4, that arose from the whole genome duplication Gene:GUP1(YGL084C)|FD-Score:3.88|P-value:5.19E-5||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HAP4(YKL109W)|FD-Score:-3.65|P-value:1.31E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex Gene:HEH2(YDR458C)|FD-Score:3.1|P-value:9.75E-4||SGD DESC:Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle Gene:HEL1(YKR017C_p)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU) Gene:IMO32(YGR031W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Conserved mitochondrial protein of unknown function; processed by both mitochondrial processing peptidase and mitochondrial octapeptidyl aminopeptidase; gene contains the nested antisense gene NAG1 Gene:KCS1(YDR017C)|FD-Score:4.04|P-value:2.63E-5||SGD DESC:Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; generation of high energy inositol pyrophosphates by Kcs1p is required for many processes such as vacuolar biogenesis, stress response and telomere maintenance Gene:LHS1(YKL073W)|FD-Score:4.18|P-value:1.45E-5||SGD DESC:Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway Gene:MET12(YPL023C)|FD-Score:-5.46|P-value:2.41E-8||SGD DESC:Protein with methylenetetrahydrofolate reductase (MTHFR) activity in vitro; null mutant has no phenotype and is prototrophic for methionine; MET13 encodes major isozyme of MTHFR Gene:MRPL11(YDL202W)|FD-Score:3.28|P-value:5.19E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL16(YBL038W)|FD-Score:4.04|P-value:2.71E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL51(YPR100W)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NDT80(YHR124W)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Meiosis-specific transcription factor required for exit from pachytene and for full meiotic recombination; activates middle sporulation genes; competes with Sum1p for binding to promoters containing middle sporulation elements (MSE) Gene:OM14(YBR230C)|FD-Score:3.15|P-value:8.28E-4||SGD DESC:Integral mitochondrial outer membrane protein; abundance is decreased in cells grown in glucose relative to other carbon sources; appears to contain 3 alpha-helical transmembrane segments; ORF encodes a 97-basepair intron Gene:OPT1(YJL212C)|FD-Score:3.8|P-value:7.33E-5||SGD DESC:Proton-coupled oligopeptide transporter of the plasma membrane; also transports glutathione and phytochelatin; member of the OPT family Gene:PCP1(YGR101W)|FD-Score:-4.66|P-value:1.60E-6||SGD DESC:Mitochondrial serine protease required for the processing of various mitochondrial proteins and maintenance of mitochondrial DNA and morphology; belongs to the rhomboid-GlpG superfamily of intramembrane peptidases Gene:PDR18(YNR070W_p)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:Putative transporter of the ATP-binding cassette (ABC) family, implicated in pleiotropic drug resistance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PEX32(YBR168W)|FD-Score:-3.91|P-value:4.70E-5||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PFK2(YMR205C)|FD-Score:3.72|P-value:9.90E-5||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PPR1(YLR014C)|FD-Score:3.24|P-value:6.03E-4||SGD DESC:Zinc finger transcription factor containing a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of URA1, URA3, URA4, and URA10, which are involved in de novo pyrimidine biosynthesis, in response to pyrimidine starvation; activity may be modulated by interaction with Tup1p Gene:PSY3(YLR376C)|FD-Score:4.22|P-value:1.20E-5||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; deletion of PSY3 results in a mutator phenotype; deletion increases sensitivity to anticancer drugs oxaliplatin and cisplatin but not mitomycin C Gene:PTC7(YHR076W)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:PTH2(YBL057C)|FD-Score:4.88|P-value:5.25E-7||SGD DESC:One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth Gene:RAD55(YDR076W)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p Gene:RGD1(YBR260C)|FD-Score:-3.43|P-value:3.01E-4||SGD DESC:GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization Gene:RNH201(YNL072W)|FD-Score:4.15|P-value:1.63E-5||SGD DESC:Ribonuclease H2 catalytic subunit, removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome Gene:RPL35B(YDL136W)|FD-Score:-3.14|P-value:8.51E-4||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RPP2A(YOL039W)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm Gene:RTS1(YOR014W)|FD-Score:4.09|P-value:2.20E-5||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:SLH1(YGR271W)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses Gene:SPF1(YEL031W)|FD-Score:-3.44|P-value:2.92E-4||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SPT21(YMR179W)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SRL2(YLR082C)|FD-Score:3.21|P-value:6.60E-4||SGD DESC:Protein of unknown function; overexpression suppresses the lethality caused by a rad53 null mutation Gene:TAN1(YGL232W)|FD-Score:-3.2|P-value:6.99E-4||SGD DESC:Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress Gene:TAT1(YBR069C)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TDA10(YGR205W)|FD-Score:-3.21|P-value:6.63E-4||SGD DESC:ATP-binding protein of unknown function; crystal structure resembles that of E.coli pantothenate kinase and other small kinases; null mutant is sensitive to expression of the top1-T722A allele Gene:TGL3(YMR313C)|FD-Score:3.1|P-value:9.52E-4||SGD DESC:Bifunctional enzyme with triacylglycerol lipase and lysophosphatidylethanolamine acyltransferase activity; responsible for all the triacylglycerol lipase activity of the lipid particle; required with Tgl4p for timely bud formation Gene:TOK1(YJL093C)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Outward-rectifier potassium channel of the plasma membrane with two pore domains in tandem, each of which forms a functional channel permeable to potassium; carboxy tail functions to prevent inner gate closures; target of K1 toxin Gene:TRM7(YBR061C)|FD-Score:-3.28|P-value:5.28E-4||SGD DESC:2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively Gene:TSR2(YLR435W)|FD-Score:4.23|P-value:1.16E-5||SGD DESC:Protein with a potential role in pre-rRNA processing Gene:TUB3(YML124C)|FD-Score:5.03|P-value:2.40E-7||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:VAM3(YOR106W)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region Gene:VBA3(YCL069W)|FD-Score:5.21|P-value:9.35E-8||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:WHI3(YNL197C)|FD-Score:3.21|P-value:6.75E-4||SGD DESC:RNA binding protein that sequesters CLN3 mRNA in cytoplasmic foci;; Tpk1 (PKA) mediated phosphorylation of Whi3 affects G1/S transition by modulating Cln3 transcription; cytoplasmic retention factor for Cdc28p and associated cyclins; regulates cell fate and dose-dependently regulates the critical cell size required for passage through Start; WHI3 has a paralog, WHI4, that arose from the whole genome duplication Gene:YAP6(YDR259C)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; overexpression increases sodium and lithium tolerance; computational analysis suggests a role in regulation of expression of genes involved in carbohydrate metabolism; YAP6 has a paralog, CIN5, that arose from the whole genome duplication Gene:YBL100W-C(YBL100W-C_p)|FD-Score:5.49|P-value:2.00E-8||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR277C(YBR277C_d)|FD-Score:3.22|P-value:6.48E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W Gene:YER010C(YER010C)|FD-Score:-3.64|P-value:1.35E-4||SGD DESC:Protein of unknown function, forms a ring-shaped homotrimer; has similarity to members of the prokaryotic rraA family; possibly involved in a phosphotransfer reaction Gene:YFR006W(YFR006W_p)|FD-Score:-3.18|P-value:7.26E-4||SGD DESC:Putative X-Pro aminopeptidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR006W is not an essential gene Gene:YGK3(YOL128C)|FD-Score:4.3|P-value:8.41E-6||SGD DESC:Protein kinase related to mammalian glycogen synthase kinases of the GSK-3 family; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation Gene:YGL007C-A(YGL007C-A_p)|FD-Score:-3.22|P-value:6.43E-4||SGD DESC:Putative protein of unknown function, identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; deletion exhibits slow-growth phenotype; computationally predicted to have a role in cell budding Gene:YGL260W(YGL260W_p)|FD-Score:-4.3|P-value:8.70E-6||SGD DESC:Putative protein of unknown function; transcription is significantly increased in a NAP1 deletion background; deletion mutant has increased accumulation of nickel and selenium Gene:YGR228W(YGR228W_d)|FD-Score:5.99|P-value:1.06E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YJL120W(YJL120W_d)|FD-Score:6.02|P-value:8.94E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YJL150W(YJL150W_d)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL027W(YKL027W)|FD-Score:3.16|P-value:7.80E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YKL115C(YKL115C_d)|FD-Score:4.02|P-value:2.90E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1 Gene:YLR012C(YLR012C_p)|FD-Score:-4.39|P-value:5.72E-6||SGD DESC:Putative protein of unknown function; YLR012C is not an essential gene Gene:YMD8(YML038C)|FD-Score:-3.52|P-value:2.18E-4||SGD DESC:Putative nucleotide sugar transporter, has similarity to Vrg4p Gene:YMR052C-A(YMR052C-A_d)|FD-Score:4.06|P-value:2.51E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR304C-A(YOR304C-A_p)|FD-Score:-5.41|P-value:3.12E-8||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck Gene:YOR325W(YOR325W_d)|FD-Score:3.19|P-value:7.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF FRT1 Gene:YPR084W(YPR084W_p)|FD-Score:3.87|P-value:5.47E-5||SGD DESC:Putative protein of unknown function

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YJR123W6.785.98E-121.65RPS5Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7
YCL004W6.222.49E-101.65PGS1Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis
YJL076W4.572.40E-60.40NET1Core subunit of the RENT complex; involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure; NET1 has a paralog, TOF2, that arose from the whole genome duplication
YNR038W4.171.53E-50.04DBP6Essential protein involved in ribosome biogenesis; putative ATP-dependent RNA helicase of the DEAD-box protein family
YOR272W4.131.80E-50.14YTM1Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats
YGL150C3.993.35E-50.06INO80ATPase and nucleosome spacing factor, subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; has a role in modulating stress gene transcription
YAL003W3.924.36E-50.44EFB1Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site
YOR340C3.492.44E-40.03RPA43RNA polymerase I subunit A43
YLR310C3.462.69E-40.04CDC25Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1
YDL031W3.423.13E-40.26DBP10Putative ATP-dependent RNA helicase of the DEAD-box protein family, constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis
YPL146C3.167.85E-40.02NOP53Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired
YKR081C3.148.42E-40.25RPF2Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles
YBR004C2.890.001950.01GPI18Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria
YER013W2.880.002003.65E-4PRP22DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes
YEL002C2.880.002000.05WBP1Beta subunit of the oligosaccharyl transferase (OST) glycoprotein complex; required for N-linked glycosylation of proteins in the endoplasmic reticulum

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJL120W_d6.028.94E-10YJL120W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO
YGR228W_d5.991.06E-9YGR228W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C
YBL100W-C_p5.492.00E-8YBL100W-C_pPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
YJR032W5.286.57E-8CPR7Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity
YCL069W5.219.35E-8VBA3Permease of basic amino acids in the vacuolar membrane
YML124C5.032.40E-7TUB3Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication
YBL057C4.885.25E-7PTH2One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth
YBR180W4.691.39E-6DTR1Putative dityrosine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for spore wall synthesis; expressed during sporulation
YOL128C4.308.41E-6YGK3Protein kinase related to mammalian glycogen synthase kinases of the GSK-3 family; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation
YLR435W4.231.16E-5TSR2Protein with a potential role in pre-rRNA processing
YLR376C4.221.20E-5PSY3Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; deletion of PSY3 results in a mutator phenotype; deletion increases sensitivity to anticancer drugs oxaliplatin and cisplatin but not mitomycin C
YKL073W4.181.45E-5LHS1Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway
YNL072W4.151.63E-5RNH201Ribonuclease H2 catalytic subunit, removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome
YOR014W4.092.20E-5RTS1B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A
YMR052C-A_d4.062.51E-5YMR052C-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

GO enrichment analysis for SGTC_1014
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0715.16E-8SGTC_5914299-0174 154.0 μMChemDiv (Drug-like library)55090880.1
0.0672.21E-7SGTC_21735763948 167.0 μMChembridge (Fragment library)28671770.0983607tubulin folding & SWR complex
0.0672.84E-7SGTC_25534-methylesculetin 85.1 μMMicrosource (Natural product library)53195020.0701754
0.0663.19E-7SGTC_1558st069300 45.6 μMTimTec (Pure natural product library)242080000.0645161
0.0656.46E-7SGTC_32949122953 26.3 μMChembridge (Drug-like library)164598260.0909091tubulin folding & SWR complex
0.0612.62E-6SGTC_2575plumbagin 510.0 nMTimTec (Pure natural product library)102050.107143superoxide
0.0612.69E-6SGTC_13521496-0991 99.0 μMChemDiv (Drug-like library)30000390.12069tubulin folding & SWR complex
0.0603.65E-6SGTC_1602st001878 87.6 μMTimTec (Natural product derivative library)5954050.129032
0.0587.20E-6SGTC_2510benzyl isothiocyanate 8.3 μMMicrosource (Natural product library)23460.1
0.0552.03E-5SGTC_5991935-0052 86.5 μMChemDiv (Drug-like library)95573110.102564
0.0543.01E-5SGTC_20895281248 129.7 μMChembridge (Fragment library)27378780.0675676superoxide
0.0543.24E-5SGTC_29969066192 71.4 μMChembridge (Drug-like library)64728540.117647
0.0543.47E-5SGTC_255413-methyl-4,4-bisnor-8,11,13-podocarpatrien-3-one 66.7 μMMicrosource (Natural product library)67085610.0757576
0.0534.08E-5SGTC_10234482-3888 16.0 μMChemDiv (Drug-like library)33031680.125TSC3-RPN4
0.0526.18E-5SGTC_24515856223 45.7 μMMiscellaneous21798960.0694444

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2788528273471.43 μM0.586957743706Chembridge (Drug-like library)283.282023.05605
SGTC_2785775646372.73 μM0.4807692201551Chembridge (Drug-like library)308.334543.33214
SGTC_8261683-5028118 μM0.346154722377ChemDiv (Drug-like library)261.703583.26212
SGTC_5651418-000144.5 μM0.3333332862145ChemDiv (Drug-like library)245.704183.63812
SGTC_13511496-000346.5 μM0.3207553891978ChemDiv (Drug-like library)330.212642.908
SGTC_2900532852885.29 μM0.320755795359Chembridge (Drug-like library)261.703583.26212Golgi
SGTC_11564092-03674.88 μM0.3050851357585ChemDiv (Drug-like library)382.926285.00702
SGTC_80354-000170 μM0.3035714993152ChemDiv (Drug-like library)354.229326.15902
SGTC_7161683-5115658 μM0.303571723597ChemDiv (Drug-like library)303.740263.48313
SGTC_3188911078949.47 μM0.303571849217Chembridge (Drug-like library)275.730163.61112iron homeostasis