4239-0030

N-(4-bromophenyl)-3-[4-(2-fluorophenyl)piperazin-1-yl]propanamide

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1017
Screen concentration 168.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 1149593
SMILES C1CN(CCN1CCC(=O)NC2=CC=C(C=C2)Br)C3=CC=CC=C3F
Standardized SMILES Fc1ccccc1N2CCN(CCC(=O)Nc3ccc(Br)cc3)CC2
Molecular weight 406.2919
ALogP 3.79
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 7.02
% growth inhibition (Hom. pool) 6.17


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1149593
Download HIP data (tab-delimited text)  (excel)
Gene:AVO1(YOL078W)|FD-Score:-4.49|P-value:3.64E-6|Clearance:0||SGD DESC:Component of a membrane-bound complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth Gene:MRPS18(YNL306W)|FD-Score:3.81|P-value:6.96E-5|Clearance:0.3||SGD DESC:Mitochondrial ribosomal protein of the small subunit; essential for viability, unlike most other mitoribosomal proteins Gene:NCB2(YDR397C)|FD-Score:-3.38|P-value:3.57E-4|Clearance:0||SGD DESC:Subunit of a heterodimeric NC2 transcription regulator complex with Bur6p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2beta Gene:POP1(YNL221C)|FD-Score:3.1|P-value:9.71E-4|Clearance:0.07||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:RNA15(YGL044C)|FD-Score:3.51|P-value:2.22E-4|Clearance:0.11||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:SDO1(YLR022C)|FD-Score:3.86|P-value:5.62E-5|Clearance:0.05||SGD DESC:Essential protein involved in ribosome maturation; with Ria1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; ortholog of the human protein (SBDS) responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome; highly conserved across archaea and eukaryotes Gene:SEC21(YNL287W)|FD-Score:-3.36|P-value:3.95E-4|Clearance:0||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:SRP102(YKL154W)|FD-Score:3.41|P-value:3.29E-4|Clearance:0.31||SGD DESC:Signal recognition particle (SRP) receptor beta subunit; involved in SRP-dependent protein targeting; anchors the alpha subunit, Srp101p to the ER membrane Gene:SRP72(YPL210C)|FD-Score:-3.2|P-value:6.90E-4|Clearance:0||SGD DESC:Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane Gene:TFC3(YAL001C)|FD-Score:-3.6|P-value:1.58E-4|Clearance:0||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:TRS130(YMR218C)|FD-Score:-3.55|P-value:1.96E-4|Clearance:0||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1; mutants are defective in cytoplasm-to-vacuole targeting (Cvt) pathway and starvation-induced autophagy Gene:TSC11(YER093C)|FD-Score:-3.86|P-value:5.62E-5|Clearance:0||SGD DESC:Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p), a membrane-associated complex that regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; involved in sphingolipid metabolism; contains a RasGEFN domain Gene:YGR114C(YGR114C_d)|FD-Score:5.87|P-value:2.24E-9|Clearance:2||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:AVO1(YOL078W)|FD-Score:-4.49|P-value:3.64E-6|Clearance:0||SGD DESC:Component of a membrane-bound complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth Gene:MRPS18(YNL306W)|FD-Score:3.81|P-value:6.96E-5|Clearance:0.3||SGD DESC:Mitochondrial ribosomal protein of the small subunit; essential for viability, unlike most other mitoribosomal proteins Gene:NCB2(YDR397C)|FD-Score:-3.38|P-value:3.57E-4|Clearance:0||SGD DESC:Subunit of a heterodimeric NC2 transcription regulator complex with Bur6p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2beta Gene:POP1(YNL221C)|FD-Score:3.1|P-value:9.71E-4|Clearance:0.07||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:RNA15(YGL044C)|FD-Score:3.51|P-value:2.22E-4|Clearance:0.11||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:SDO1(YLR022C)|FD-Score:3.86|P-value:5.62E-5|Clearance:0.05||SGD DESC:Essential protein involved in ribosome maturation; with Ria1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; ortholog of the human protein (SBDS) responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome; highly conserved across archaea and eukaryotes Gene:SEC21(YNL287W)|FD-Score:-3.36|P-value:3.95E-4|Clearance:0||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:SRP102(YKL154W)|FD-Score:3.41|P-value:3.29E-4|Clearance:0.31||SGD DESC:Signal recognition particle (SRP) receptor beta subunit; involved in SRP-dependent protein targeting; anchors the alpha subunit, Srp101p to the ER membrane Gene:SRP72(YPL210C)|FD-Score:-3.2|P-value:6.90E-4|Clearance:0||SGD DESC:Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane Gene:TFC3(YAL001C)|FD-Score:-3.6|P-value:1.58E-4|Clearance:0||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:TRS130(YMR218C)|FD-Score:-3.55|P-value:1.96E-4|Clearance:0||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1; mutants are defective in cytoplasm-to-vacuole targeting (Cvt) pathway and starvation-induced autophagy Gene:TSC11(YER093C)|FD-Score:-3.86|P-value:5.62E-5|Clearance:0||SGD DESC:Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p), a membrane-associated complex that regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; involved in sphingolipid metabolism; contains a RasGEFN domain Gene:YGR114C(YGR114C_d)|FD-Score:5.87|P-value:2.24E-9|Clearance:2||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1149593
Download HOP data (tab-delimited text)  (excel)
Gene:AEP2(YMR282C)|FD-Score:3.94|P-value:4.04E-5||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:AEP3(YPL005W)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Protein that may facilitate use of unformylated tRNA-Met in mitochondrial translation initiation; localized to the matrix face of the mitochondrial inner membrane; stabilizes the bicistronic AAP1-ATP6 mRNA Gene:AIM37(YNL100W)|FD-Score:-3.11|P-value:9.25E-4||SGD DESC:Mitochondrial inner membrane protein; component of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:BIT2(YBR270C_p)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Subunit of TORC2 membrane-associated complex; involved in regulation of actin cytoskeletal dynamics during polarized growth and cell wall integrity; interacts with Slm1p and Slm2p, homologous PH domain-containing TORC2 substrates; BIT2 has a paralog, BIT61, that arose from the whole genome duplication Gene:CCE1(YKL011C)|FD-Score:-3.96|P-value:3.70E-5||SGD DESC:Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA Gene:CIT1(YNR001C)|FD-Score:-3.13|P-value:8.64E-4||SGD DESC:Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication Gene:CIT3(YPR001W)|FD-Score:3.23|P-value:6.22E-4||SGD DESC:Dual specificity mitochondrial citrate and methylcitrate synthase; catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and that of propionyl-CoA and oxaloacetate to form 2-methylcitrate Gene:CTF4(YPR135W)|FD-Score:4.64|P-value:1.71E-6||SGD DESC:Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion Gene:CYS3(YAL012W)|FD-Score:-3.23|P-value:6.13E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:EST2(YLR318W)|FD-Score:4.77|P-value:9.26E-7||SGD DESC:Reverse transcriptase subunit of the telomerase holoenzyme, essential for telomerase core catalytic activity, involved in other aspects of telomerase assembly and function; mutations in human homolog are associated with aplastic anemia Gene:FMP46(YKR049C)|FD-Score:-3.19|P-value:7.08E-4||SGD DESC:Putative redox protein containing a thioredoxin fold; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FYV5(YCL058C)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Protein involved in regulation of the mating pathway; binds with Matalpha2p to promoters of haploid-specific genes; required for survival upon exposure to K1 killer toxin; involved in ion homeostasis Gene:GND2(YGR256W)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone; GND2 has a paralog, GND1, that arose from the whole genome duplication Gene:HOR7(YMR251W-A)|FD-Score:3.87|P-value:5.50E-5||SGD DESC:Protein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor; HOR7 has a paralog, DDR2, that arose from the whole genome duplication Gene:IRC2(YDR112W_d)|FD-Score:3.23|P-value:6.20E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR111C; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRS4(YKR019C)|FD-Score:-3.27|P-value:5.43E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:IXR1(YKL032C)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repression of COX5b Gene:KAP123(YER110C)|FD-Score:4.54|P-value:2.80E-6||SGD DESC:Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1 Gene:KAR9(YPL269W)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase Gene:LEM3(YNL323W)|FD-Score:-3.29|P-value:5.04E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LRO1(YNR008W)|FD-Score:-3.32|P-value:4.47E-4||SGD DESC:Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase Gene:MAM33(YIL070C)|FD-Score:4.98|P-value:3.20E-7||SGD DESC:Acidic protein of the mitochondrial matrix involved in oxidative phosphorylation; related to the human complement receptor gC1q-R Gene:MCM16(YPR046W)|FD-Score:5.31|P-value:5.53E-8||SGD DESC:Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3 Gene:MGM101(YJR144W)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Protein with a role in mitochondrial DNA recombinational repair;also involved in interstrand cross-link repair; binds to and catalyzes the annealing of single-stranded mtDNA; oligomerizes to form rings and filaments; related to Rad52-type recombination proteins, with limited overall similarity but sharing conserved functionally important residues; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage Gene:MMP1(YLL061W)|FD-Score:-3.35|P-value:3.99E-4||SGD DESC:High-affinity S-methylmethionine permease, required for utilization of S-methylmethionine as a sulfur source; has similarity to S-adenosylmethionine permease Sam3p Gene:MRE11(YMR224C)|FD-Score:6.59|P-value:2.19E-11||SGD DESC:Subunit of the MRX complex with Rad50p and Xrs2p; complex functions in repair of DNA double-strand breaks and in telomere stability; exhibits nuclease activity that appears to be required for MRX function; widely conserved; forms nuclear foci upon DNA replication stress Gene:MRPL51(YPR100W)|FD-Score:3.24|P-value:5.97E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSH3(YCR092C)|FD-Score:3.73|P-value:9.42E-5||SGD DESC:Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability Gene:MSS116(YDR194C)|FD-Score:5.72|P-value:5.20E-9||SGD DESC:DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing Gene:MTC4(YBR255W)|FD-Score:3.87|P-value:5.35E-5||SGD DESC:Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 Gene:OCA1(YNL099C)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA Gene:OPI9(YLR338W_d)|FD-Score:-3.64|P-value:1.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:PAU5(YFL020C)|FD-Score:-3.31|P-value:4.64E-4||SGD DESC:Member of the seripauperin multigene family encoded mainly in subtelomeric regions; induced during alcoholic fermentation; induced by low temperature and also by anaerobic conditions; negatively regulated by oxygen and repressed by heme Gene:PCL9(YDL179W)|FD-Score:-3.11|P-value:9.49E-4||SGD DESC:Cyclin, forms a functional kinase complex with Pho85p cyclin-dependent kinase (Cdk), expressed in late M/early G1 phase, activated by Swi5p Gene:PET111(YMR257C)|FD-Score:3.16|P-value:7.85E-4||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:PET309(YLR067C)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Specific translational activator for the COX1 mRNA, also influences stability of intron-containing COX1 primary transcripts; localizes to the mitochondrial inner membrane; contains seven pentatricopeptide repeats (PPRs) Gene:PIH1(YHR034C)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II Gene:RAD51(YER095W)|FD-Score:3.12|P-value:9.15E-4||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RAV1(YJR033C)|FD-Score:3.75|P-value:8.76E-5||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:RCO1(YMR075W)|FD-Score:4.75|P-value:1.02E-6||SGD DESC:Essential subunit of the histone deacetylase Rpd3S complex; interacts with Eaf3p Gene:REC107(YJR021C)|FD-Score:4.7|P-value:1.28E-6||SGD DESC:Protein involved in early stages of meiotic recombination; involved in coordination between the initiation of recombination and the first division of meiosis; part of a complex (Rec107p-Mei4p-Rec114p) required for ds break formation Gene:RHO4(YKR055W)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely to be involved in the establishment of cell polarity Gene:RLM1(YPL089C)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:MADS-box transcription factor; component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p; RLM1 has a paralog, SMP1, that arose from the whole genome duplication Gene:RML2(YEL050C)|FD-Score:6.22|P-value:2.46E-10||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:RPL22A(YLR061W)|FD-Score:3.21|P-value:6.54E-4||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RPP2A(YOL039W)|FD-Score:3.87|P-value:5.47E-5||SGD DESC:Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm Gene:RPS14A(YCR031C)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication Gene:RPS16A(YMR143W)|FD-Score:3.84|P-value:6.25E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16A has a paralog, RPS16B, that arose from the whole genome duplication Gene:RRG7(YOR305W)|FD-Score:6.52|P-value:3.54E-11||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YOR305W is not an essential gene Gene:RTN2(YDL204W)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; much less abundant than Rtn1p; rtn1 rtn2 yop1 triple mutant lacks tubular ER; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress; RTN2 has a paralog, RTN1, that arose from the whole genome duplication Gene:RUP1(YOR138C)|FD-Score:4.56|P-value:2.52E-6||SGD DESC:Protein that regulates Rsp5p, which is a HECT ubiquitin ligase; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress Gene:SER3(YER081W)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER3 has a paralog, SER33, that arose from the whole genome duplication Gene:SFP1(YLR403W)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion Gene:SLM3(YDL033C)|FD-Score:3.81|P-value:6.96E-5||SGD DESC:tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF) Gene:SLS1(YLR139C)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SMI1(YGR229C)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:THP1(YOL072W)|FD-Score:-3.24|P-value:6.04E-4||SGD DESC:Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding Gene:TNA1(YGR260W)|FD-Score:3.88|P-value:5.14E-5||SGD DESC:High affinity nicotinic acid plasma membrane permease, responsible for uptake of low levels of nicotinic acid; expression of the gene increases in the absence of extracellular nicotinic acid or para-aminobenzoate (PABA) Gene:TRM9(YML014W)|FD-Score:-3.41|P-value:3.26E-4||SGD DESC:tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses Gene:TSR2(YLR435W)|FD-Score:3.25|P-value:5.75E-4||SGD DESC:Protein with a potential role in pre-rRNA processing Gene:UBP6(YFR010W)|FD-Score:4.18|P-value:1.45E-5||SGD DESC:Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance Gene:UBP7(YIL156W)|FD-Score:-3.74|P-value:9.12E-5||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin-protein fusions Gene:YBL029W(YBL029W_p)|FD-Score:4.46|P-value:4.17E-6||SGD DESC:Non-essential protein of unknown function Gene:YBR206W(YBR206W_d)|FD-Score:3.96|P-value:3.77E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KTR3 Gene:YBR238C(YBR238C)|FD-Score:4.19|P-value:1.38E-5||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YCL049C(YCL049C_p)|FD-Score:4.01|P-value:3.05E-5||SGD DESC:Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene Gene:YCR023C(YCR023C)|FD-Score:3.93|P-value:4.28E-5||SGD DESC:Vacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene Gene:YER010C(YER010C)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:Protein of unknown function, forms a ring-shaped homotrimer; has similarity to members of the prokaryotic rraA family; possibly involved in a phosphotransfer reaction Gene:YER077C(YER077C_p)|FD-Score:5.11|P-value:1.58E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport Gene:YGR015C(YGR015C_p)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:YHP1(YDR451C)|FD-Score:-4.05|P-value:2.57E-5||SGD DESC:Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication Gene:YHR202W(YHR202W_p)|FD-Score:4.29|P-value:9.08E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization Gene:YKR018C(YKR018C_p)|FD-Score:3.13|P-value:8.69E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication Gene:YLL007C(YLL007C_p)|FD-Score:-3.13|P-value:8.83E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YLR122C(YLR122C_d)|FD-Score:-3.63|P-value:1.44E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR123C Gene:YLR202C(YLR202C_d)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron Gene:YLR358C(YLR358C_p)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps ORF RSC2/YLR357W Gene:YMR075C-A(YMR075C-A_d)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RCO1/YMR075W Gene:YMR193C-A(YMR193C-A_d)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL226W(YNL226W_d)|FD-Score:3.11|P-value:9.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C Gene:YOR015W(YOR015W_d)|FD-Score:-3.16|P-value:7.89E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR248W(YOR248W_d)|FD-Score:7.07|P-value:7.49E-13||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR304C-A(YOR304C-A_p)|FD-Score:5.04|P-value:2.34E-7||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck Gene:YPC1(YBR183W)|FD-Score:3.42|P-value:3.19E-4||SGD DESC:Alkaline ceramidase; also has reverse (CoA-independent) ceramide synthase activity; catalyzes both breakdown and synthesis of phytoceramide; overexpression confers fumonisin B1 resistance; YPC1 has a paralog, YDC1, that arose from the whole genome duplication Gene:YPL119C-A(YPL119C-A_p)|FD-Score:-4.27|P-value:9.57E-6||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YPR195C(YPR195C_d)|FD-Score:-3.46|P-value:2.69E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:AEP2(YMR282C)|FD-Score:3.94|P-value:4.04E-5||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:AEP3(YPL005W)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Protein that may facilitate use of unformylated tRNA-Met in mitochondrial translation initiation; localized to the matrix face of the mitochondrial inner membrane; stabilizes the bicistronic AAP1-ATP6 mRNA Gene:AIM37(YNL100W)|FD-Score:-3.11|P-value:9.25E-4||SGD DESC:Mitochondrial inner membrane protein; component of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:BIT2(YBR270C_p)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Subunit of TORC2 membrane-associated complex; involved in regulation of actin cytoskeletal dynamics during polarized growth and cell wall integrity; interacts with Slm1p and Slm2p, homologous PH domain-containing TORC2 substrates; BIT2 has a paralog, BIT61, that arose from the whole genome duplication Gene:CCE1(YKL011C)|FD-Score:-3.96|P-value:3.70E-5||SGD DESC:Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA Gene:CIT1(YNR001C)|FD-Score:-3.13|P-value:8.64E-4||SGD DESC:Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication Gene:CIT3(YPR001W)|FD-Score:3.23|P-value:6.22E-4||SGD DESC:Dual specificity mitochondrial citrate and methylcitrate synthase; catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and that of propionyl-CoA and oxaloacetate to form 2-methylcitrate Gene:CTF4(YPR135W)|FD-Score:4.64|P-value:1.71E-6||SGD DESC:Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion Gene:CYS3(YAL012W)|FD-Score:-3.23|P-value:6.13E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:EST2(YLR318W)|FD-Score:4.77|P-value:9.26E-7||SGD DESC:Reverse transcriptase subunit of the telomerase holoenzyme, essential for telomerase core catalytic activity, involved in other aspects of telomerase assembly and function; mutations in human homolog are associated with aplastic anemia Gene:FMP46(YKR049C)|FD-Score:-3.19|P-value:7.08E-4||SGD DESC:Putative redox protein containing a thioredoxin fold; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FYV5(YCL058C)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Protein involved in regulation of the mating pathway; binds with Matalpha2p to promoters of haploid-specific genes; required for survival upon exposure to K1 killer toxin; involved in ion homeostasis Gene:GND2(YGR256W)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone; GND2 has a paralog, GND1, that arose from the whole genome duplication Gene:HOR7(YMR251W-A)|FD-Score:3.87|P-value:5.50E-5||SGD DESC:Protein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor; HOR7 has a paralog, DDR2, that arose from the whole genome duplication Gene:IRC2(YDR112W_d)|FD-Score:3.23|P-value:6.20E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR111C; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRS4(YKR019C)|FD-Score:-3.27|P-value:5.43E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:IXR1(YKL032C)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repression of COX5b Gene:KAP123(YER110C)|FD-Score:4.54|P-value:2.80E-6||SGD DESC:Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1 Gene:KAR9(YPL269W)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase Gene:LEM3(YNL323W)|FD-Score:-3.29|P-value:5.04E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LRO1(YNR008W)|FD-Score:-3.32|P-value:4.47E-4||SGD DESC:Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase Gene:MAM33(YIL070C)|FD-Score:4.98|P-value:3.20E-7||SGD DESC:Acidic protein of the mitochondrial matrix involved in oxidative phosphorylation; related to the human complement receptor gC1q-R Gene:MCM16(YPR046W)|FD-Score:5.31|P-value:5.53E-8||SGD DESC:Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3 Gene:MGM101(YJR144W)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Protein with a role in mitochondrial DNA recombinational repair;also involved in interstrand cross-link repair; binds to and catalyzes the annealing of single-stranded mtDNA; oligomerizes to form rings and filaments; related to Rad52-type recombination proteins, with limited overall similarity but sharing conserved functionally important residues; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage Gene:MMP1(YLL061W)|FD-Score:-3.35|P-value:3.99E-4||SGD DESC:High-affinity S-methylmethionine permease, required for utilization of S-methylmethionine as a sulfur source; has similarity to S-adenosylmethionine permease Sam3p Gene:MRE11(YMR224C)|FD-Score:6.59|P-value:2.19E-11||SGD DESC:Subunit of the MRX complex with Rad50p and Xrs2p; complex functions in repair of DNA double-strand breaks and in telomere stability; exhibits nuclease activity that appears to be required for MRX function; widely conserved; forms nuclear foci upon DNA replication stress Gene:MRPL51(YPR100W)|FD-Score:3.24|P-value:5.97E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSH3(YCR092C)|FD-Score:3.73|P-value:9.42E-5||SGD DESC:Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability Gene:MSS116(YDR194C)|FD-Score:5.72|P-value:5.20E-9||SGD DESC:DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing Gene:MTC4(YBR255W)|FD-Score:3.87|P-value:5.35E-5||SGD DESC:Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 Gene:OCA1(YNL099C)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA Gene:OPI9(YLR338W_d)|FD-Score:-3.64|P-value:1.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:PAU5(YFL020C)|FD-Score:-3.31|P-value:4.64E-4||SGD DESC:Member of the seripauperin multigene family encoded mainly in subtelomeric regions; induced during alcoholic fermentation; induced by low temperature and also by anaerobic conditions; negatively regulated by oxygen and repressed by heme Gene:PCL9(YDL179W)|FD-Score:-3.11|P-value:9.49E-4||SGD DESC:Cyclin, forms a functional kinase complex with Pho85p cyclin-dependent kinase (Cdk), expressed in late M/early G1 phase, activated by Swi5p Gene:PET111(YMR257C)|FD-Score:3.16|P-value:7.85E-4||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:PET309(YLR067C)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Specific translational activator for the COX1 mRNA, also influences stability of intron-containing COX1 primary transcripts; localizes to the mitochondrial inner membrane; contains seven pentatricopeptide repeats (PPRs) Gene:PIH1(YHR034C)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II Gene:RAD51(YER095W)|FD-Score:3.12|P-value:9.15E-4||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RAV1(YJR033C)|FD-Score:3.75|P-value:8.76E-5||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:RCO1(YMR075W)|FD-Score:4.75|P-value:1.02E-6||SGD DESC:Essential subunit of the histone deacetylase Rpd3S complex; interacts with Eaf3p Gene:REC107(YJR021C)|FD-Score:4.7|P-value:1.28E-6||SGD DESC:Protein involved in early stages of meiotic recombination; involved in coordination between the initiation of recombination and the first division of meiosis; part of a complex (Rec107p-Mei4p-Rec114p) required for ds break formation Gene:RHO4(YKR055W)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely to be involved in the establishment of cell polarity Gene:RLM1(YPL089C)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:MADS-box transcription factor; component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p; RLM1 has a paralog, SMP1, that arose from the whole genome duplication Gene:RML2(YEL050C)|FD-Score:6.22|P-value:2.46E-10||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:RPL22A(YLR061W)|FD-Score:3.21|P-value:6.54E-4||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RPP2A(YOL039W)|FD-Score:3.87|P-value:5.47E-5||SGD DESC:Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm Gene:RPS14A(YCR031C)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication Gene:RPS16A(YMR143W)|FD-Score:3.84|P-value:6.25E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16A has a paralog, RPS16B, that arose from the whole genome duplication Gene:RRG7(YOR305W)|FD-Score:6.52|P-value:3.54E-11||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YOR305W is not an essential gene Gene:RTN2(YDL204W)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; much less abundant than Rtn1p; rtn1 rtn2 yop1 triple mutant lacks tubular ER; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress; RTN2 has a paralog, RTN1, that arose from the whole genome duplication Gene:RUP1(YOR138C)|FD-Score:4.56|P-value:2.52E-6||SGD DESC:Protein that regulates Rsp5p, which is a HECT ubiquitin ligase; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress Gene:SER3(YER081W)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER3 has a paralog, SER33, that arose from the whole genome duplication Gene:SFP1(YLR403W)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion Gene:SLM3(YDL033C)|FD-Score:3.81|P-value:6.96E-5||SGD DESC:tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF) Gene:SLS1(YLR139C)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SMI1(YGR229C)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:THP1(YOL072W)|FD-Score:-3.24|P-value:6.04E-4||SGD DESC:Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding Gene:TNA1(YGR260W)|FD-Score:3.88|P-value:5.14E-5||SGD DESC:High affinity nicotinic acid plasma membrane permease, responsible for uptake of low levels of nicotinic acid; expression of the gene increases in the absence of extracellular nicotinic acid or para-aminobenzoate (PABA) Gene:TRM9(YML014W)|FD-Score:-3.41|P-value:3.26E-4||SGD DESC:tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses Gene:TSR2(YLR435W)|FD-Score:3.25|P-value:5.75E-4||SGD DESC:Protein with a potential role in pre-rRNA processing Gene:UBP6(YFR010W)|FD-Score:4.18|P-value:1.45E-5||SGD DESC:Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance Gene:UBP7(YIL156W)|FD-Score:-3.74|P-value:9.12E-5||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin-protein fusions Gene:YBL029W(YBL029W_p)|FD-Score:4.46|P-value:4.17E-6||SGD DESC:Non-essential protein of unknown function Gene:YBR206W(YBR206W_d)|FD-Score:3.96|P-value:3.77E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KTR3 Gene:YBR238C(YBR238C)|FD-Score:4.19|P-value:1.38E-5||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YCL049C(YCL049C_p)|FD-Score:4.01|P-value:3.05E-5||SGD DESC:Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene Gene:YCR023C(YCR023C)|FD-Score:3.93|P-value:4.28E-5||SGD DESC:Vacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene Gene:YER010C(YER010C)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:Protein of unknown function, forms a ring-shaped homotrimer; has similarity to members of the prokaryotic rraA family; possibly involved in a phosphotransfer reaction Gene:YER077C(YER077C_p)|FD-Score:5.11|P-value:1.58E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport Gene:YGR015C(YGR015C_p)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:YHP1(YDR451C)|FD-Score:-4.05|P-value:2.57E-5||SGD DESC:Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication Gene:YHR202W(YHR202W_p)|FD-Score:4.29|P-value:9.08E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization Gene:YKR018C(YKR018C_p)|FD-Score:3.13|P-value:8.69E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication Gene:YLL007C(YLL007C_p)|FD-Score:-3.13|P-value:8.83E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YLR122C(YLR122C_d)|FD-Score:-3.63|P-value:1.44E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR123C Gene:YLR202C(YLR202C_d)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron Gene:YLR358C(YLR358C_p)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps ORF RSC2/YLR357W Gene:YMR075C-A(YMR075C-A_d)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RCO1/YMR075W Gene:YMR193C-A(YMR193C-A_d)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL226W(YNL226W_d)|FD-Score:3.11|P-value:9.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C Gene:YOR015W(YOR015W_d)|FD-Score:-3.16|P-value:7.89E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR248W(YOR248W_d)|FD-Score:7.07|P-value:7.49E-13||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR304C-A(YOR304C-A_p)|FD-Score:5.04|P-value:2.34E-7||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck Gene:YPC1(YBR183W)|FD-Score:3.42|P-value:3.19E-4||SGD DESC:Alkaline ceramidase; also has reverse (CoA-independent) ceramide synthase activity; catalyzes both breakdown and synthesis of phytoceramide; overexpression confers fumonisin B1 resistance; YPC1 has a paralog, YDC1, that arose from the whole genome duplication Gene:YPL119C-A(YPL119C-A_p)|FD-Score:-4.27|P-value:9.57E-6||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YPR195C(YPR195C_d)|FD-Score:-3.46|P-value:2.69E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGR114C_d5.872.24E-92.00YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
YLR022C3.865.62E-50.05SDO1Essential protein involved in ribosome maturation; with Ria1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; ortholog of the human protein (SBDS) responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome; highly conserved across archaea and eukaryotes
YNL306W3.816.96E-50.30MRPS18Mitochondrial ribosomal protein of the small subunit; essential for viability, unlike most other mitoribosomal proteins
YGL044C3.512.22E-40.11RNA15Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping
YKL154W3.413.29E-40.31SRP102Signal recognition particle (SRP) receptor beta subunit; involved in SRP-dependent protein targeting; anchors the alpha subunit, Srp101p to the ER membrane
YNL221C3.109.71E-40.07POP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
YKL013C3.030.001230.11ARC19Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
YDL028C2.920.001750.10MPS1Dual-specificity kinase; required for spindle pole body (SPB) duplication and spindle checkpoint function; substrates include SPB proteins Spc42p, Spc110p, and Spc98p, mitotic exit network protein Mob1p, kinetochore protein Cnn1p, and checkpoint protein Mad1p
YJL011C2.820.002370.11RPC17RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress
YBR257W2.720.003310.03POP4Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
YBL074C2.680.003630.05AAR2Component of the U5 snRNP, required for splicing of U3 precursors; originally described as a splicing factor specifically required for splicing pre-mRNA of the MATa1 cistron
YIR008C2.630.004230.01PRI1Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair
YAL003W2.620.004340.02EFB1Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site
YDL058W2.610.004580.08USO1Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex
YLR086W2.530.005690.02SMC4Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOR248W_d7.077.49E-13YOR248W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YMR224C6.592.19E-11MRE11Subunit of the MRX complex with Rad50p and Xrs2p; complex functions in repair of DNA double-strand breaks and in telomere stability; exhibits nuclease activity that appears to be required for MRX function; widely conserved; forms nuclear foci upon DNA replication stress
YOR305W6.523.54E-11RRG7Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YOR305W is not an essential gene
YEL050C6.222.46E-10RML2Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor
YDR194C5.725.20E-9MSS116DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing
YPR046W5.315.53E-8MCM16Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3
YER077C_p5.111.58E-7YER077C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport
YOR304C-A_p5.042.34E-7YOR304C-A_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck
YIL070C4.983.20E-7MAM33Acidic protein of the mitochondrial matrix involved in oxidative phosphorylation; related to the human complement receptor gC1q-R
YLR318W4.779.26E-7EST2Reverse transcriptase subunit of the telomerase holoenzyme, essential for telomerase core catalytic activity, involved in other aspects of telomerase assembly and function; mutations in human homolog are associated with aplastic anemia
YMR075W4.751.02E-6RCO1Essential subunit of the histone deacetylase Rpd3S complex; interacts with Eaf3p
YJR021C4.701.28E-6REC107Protein involved in early stages of meiotic recombination; involved in coordination between the initiation of recombination and the first division of meiosis; part of a complex (Rec107p-Mei4p-Rec114p) required for ds break formation
YPR135W4.641.71E-6CTF4Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion
YOR138C4.562.52E-6RUP1Protein that regulates Rsp5p, which is a HECT ubiquitin ligase; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress
YER110C4.542.80E-6KAP123Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1

GO enrichment analysis for SGTC_1017
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0849.06E-11SGTC_3101273-0058 1.1 μMChemDiv (Drug-like library)27342320.030303mitochondrial stress
0.0815.72E-10SGTC_8324083-0019 164.0 μMChemDiv (Drug-like library)19907580.166667translation
0.0781.90E-9SGTC_1731273-0061 2.7 μMChemDiv (Drug-like library)28474140.084507mitochondrial stress
0.0765.45E-9SGTC_6010986-0029 62.9 μMChemDiv (Drug-like library)247479810.161765calcium & mitochondrial duress
0.0732.05E-8SGTC_1180083-0097 135.3 μMChemDiv (Drug-like library)52073560.1translation
0.0655.07E-7SGTC_630099-0242 26.7 μMChemDiv (Drug-like library)38985020.0454545mitochondrial stress
0.0649.24E-7SGTC_7473448-8087 38.7 μMChemDiv (Drug-like library)51687030.121951
0.0641.02E-6SGTC_28pentachloropyridine N-oxide 1.6 μMChemDiv (Drug-like library)1402500.0181818TSC3-RPN4
0.0621.61E-6SGTC_9443370-0224 231.0 μMChemDiv (Drug-like library)45805140.149254
0.0612.59E-6SGTC_7670929-0023 86.0 μMChemDiv (Drug-like library)12045630.0810811TRP & mitochondrial translation
0.0612.81E-6SGTC_1742st038235 28.9 μMTimTec (Natural product derivative library)5641030.0963855mitochondrial stress
0.0613.18E-6SGTC_3141671-0008 2.7 μMChemDiv (Drug-like library)39130600.0632911
0.0594.89E-6SGTC_100279-0212 42.6 μMChemDiv (Drug-like library)398510.0461538TRP & mitochondrial translation
0.0587.87E-6SGTC_484loperamide 105.0 μMMiscellaneous39550.197368
0.0534.66E-5SGTC_29969066192 71.4 μMChembridge (Drug-like library)64728540.105263

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_14454239-015697.7 μM0.5862074008346ChemDiv (Drug-like library)375.4123263.22816
SGTC_14544239-005442.6 μM0.5409841340767ChemDiv (Drug-like library)432.354044.05214
SGTC_14464239-019062 μM0.4920631340085ChemDiv (Drug-like library)379.495263.87414
SGTC_2939904457871.43 μM0.4307692653634Chembridge (Drug-like library)377.8403433.43915
SGTC_14474239-081681.9 μM0.4285711341554ChemDiv (Drug-like library)367.4597433.41814
SGTC_22216807166181.08 μM0.385965674925Chembridge (Fragment library)274.2902032.47404
SGTC_3199911131849.47 μM0.3758548020Chembridge (Drug-like library)381.3672733.73317
SGTC_3284910817164.86 μM0.3731348687505Chembridge (Drug-like library)373.876463.7214
SGTC_3202911376149.47 μM0.3676474798415Chembridge (Drug-like library)377.8403433.43915
SGTC_3010908008971.43 μM0.35384617027233Chembridge (Drug-like library)378.2387833.49714