4259-0013

4-chloro-6-[5-(4-hydroxy-3-methoxyphenyl)pyrazolidin-3-ylidene]cyclohexa-2,4-dien-1-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1018
Screen concentration 28.1 μM
Source ChemDiv (Drug-like library)
PubChem CID 6801262
SMILES COC1=C(C=CC(=C1)C2CC(=C3C=C(C=CC3=O)Cl)NN2)O
Standardized SMILES COc1cc(ccc1O)C2CC(=NN2)c3cc(Cl)ccc3O
Molecular weight 318.7549
ALogP 1.45
H-bond donor count 3
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 11.04
% growth inhibition (Hom. pool) 9.34


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6801262
Download HIP data (tab-delimited text)  (excel)
Gene:AAR2(YBL074C)|FD-Score:4.37|P-value:6.28E-6|Clearance:0.46||SGD DESC:Component of the U5 snRNP, required for splicing of U3 precursors; originally described as a splicing factor specifically required for splicing pre-mRNA of the MATa1 cistron Gene:BRF1(YGR246C)|FD-Score:-3.16|P-value:7.92E-4|Clearance:0||SGD DESC:TFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB Gene:CDC5(YMR001C)|FD-Score:-3.16|P-value:7.90E-4|Clearance:0||SGD DESC:Polo-like kinase with multiple functions in mitosis and cytokinesis through substrate phosphorylation, also functions in adaptation to DNA damage during meiosis; has similarity to Xenopus Plx1 and S. pombe Plo1p; possible Cdc28p substrate Gene:DHR2(YKL078W)|FD-Score:3.71|P-value:1.03E-4|Clearance:0.01||SGD DESC:Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis Gene:ENP2(YGR145W)|FD-Score:3.1|P-value:9.77E-4|Clearance:0.12||SGD DESC:Essential nucleolar protein, required for biogenesis of the small ribosomal subunit; contains WD repeats, interacts with Mpp10p and Bfr2p, and has homology to Spb1p Gene:FBA1(YKL060C)|FD-Score:-3.69|P-value:1.11E-4|Clearance:0||SGD DESC:Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress Gene:FIP1(YJR093C)|FD-Score:3.91|P-value:4.67E-5|Clearance:0.2||SGD DESC:Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p Gene:MAK5(YBR142W)|FD-Score:3.19|P-value:7.06E-4|Clearance:0||SGD DESC:Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits Gene:MTR10(YOR160W)|FD-Score:3.31|P-value:4.64E-4|Clearance:0.12||SGD DESC:Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:NOP9(YJL010C)|FD-Score:4.46|P-value:4.12E-6|Clearance:0.09||SGD DESC:Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain Gene:OST1(YJL002C)|FD-Score:-3.26|P-value:5.48E-4|Clearance:0||SGD DESC:Alpha subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:PAB1(YER165W)|FD-Score:3.19|P-value:7.16E-4|Clearance:0.06||SGD DESC:Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G Gene:POP8(YBL018C)|FD-Score:3.13|P-value:8.77E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RAD3(YER171W)|FD-Score:-3.43|P-value:2.97E-4|Clearance:0||SGD DESC:5' to 3' DNA helicase; involved in nucleotide excision repair and transcription; subunit of RNA polII initiation factor TFIIH and of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPD protein; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress Gene:RPC17(YJL011C)|FD-Score:3.91|P-value:4.64E-5|Clearance:0||SGD DESC:RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress Gene:RPC53(YDL150W)|FD-Score:4.65|P-value:1.63E-6|Clearance:0.2||SGD DESC:RNA polymerase III subunit C53 Gene:RRP7(YCL031C)|FD-Score:5.67|P-value:7.02E-9|Clearance:0.84||SGD DESC:Essential protein involved in rRNA processing and ribosome biogenesis; protein abundance increases in response to DNA replication stress Gene:SHR3(YDL212W)|FD-Score:-3.87|P-value:5.47E-5|Clearance:0||SGD DESC:Endoplasmic reticulum packaging chaperone, required for incorporation of amino acid permeases into COPII coated vesicles for transport to the cell surface Gene:SPB4(YFL002C)|FD-Score:3.7|P-value:1.08E-4|Clearance:0.39||SGD DESC:Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients Gene:TFC3(YAL001C)|FD-Score:4.83|P-value:6.71E-7|Clearance:0.18||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:TFG1(YGR186W)|FD-Score:-3.73|P-value:9.70E-5|Clearance:0||SGD DESC:TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74 Gene:TOM40(YMR203W)|FD-Score:3.13|P-value:8.82E-4|Clearance:0.03||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:AAR2(YBL074C)|FD-Score:4.37|P-value:6.28E-6|Clearance:0.46||SGD DESC:Component of the U5 snRNP, required for splicing of U3 precursors; originally described as a splicing factor specifically required for splicing pre-mRNA of the MATa1 cistron Gene:BRF1(YGR246C)|FD-Score:-3.16|P-value:7.92E-4|Clearance:0||SGD DESC:TFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB Gene:CDC5(YMR001C)|FD-Score:-3.16|P-value:7.90E-4|Clearance:0||SGD DESC:Polo-like kinase with multiple functions in mitosis and cytokinesis through substrate phosphorylation, also functions in adaptation to DNA damage during meiosis; has similarity to Xenopus Plx1 and S. pombe Plo1p; possible Cdc28p substrate Gene:DHR2(YKL078W)|FD-Score:3.71|P-value:1.03E-4|Clearance:0.01||SGD DESC:Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis Gene:ENP2(YGR145W)|FD-Score:3.1|P-value:9.77E-4|Clearance:0.12||SGD DESC:Essential nucleolar protein, required for biogenesis of the small ribosomal subunit; contains WD repeats, interacts with Mpp10p and Bfr2p, and has homology to Spb1p Gene:FBA1(YKL060C)|FD-Score:-3.69|P-value:1.11E-4|Clearance:0||SGD DESC:Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress Gene:FIP1(YJR093C)|FD-Score:3.91|P-value:4.67E-5|Clearance:0.2||SGD DESC:Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p Gene:MAK5(YBR142W)|FD-Score:3.19|P-value:7.06E-4|Clearance:0||SGD DESC:Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits Gene:MTR10(YOR160W)|FD-Score:3.31|P-value:4.64E-4|Clearance:0.12||SGD DESC:Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:NOP9(YJL010C)|FD-Score:4.46|P-value:4.12E-6|Clearance:0.09||SGD DESC:Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain Gene:OST1(YJL002C)|FD-Score:-3.26|P-value:5.48E-4|Clearance:0||SGD DESC:Alpha subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:PAB1(YER165W)|FD-Score:3.19|P-value:7.16E-4|Clearance:0.06||SGD DESC:Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G Gene:POP8(YBL018C)|FD-Score:3.13|P-value:8.77E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RAD3(YER171W)|FD-Score:-3.43|P-value:2.97E-4|Clearance:0||SGD DESC:5' to 3' DNA helicase; involved in nucleotide excision repair and transcription; subunit of RNA polII initiation factor TFIIH and of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPD protein; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress Gene:RPC17(YJL011C)|FD-Score:3.91|P-value:4.64E-5|Clearance:0||SGD DESC:RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress Gene:RPC53(YDL150W)|FD-Score:4.65|P-value:1.63E-6|Clearance:0.2||SGD DESC:RNA polymerase III subunit C53 Gene:RRP7(YCL031C)|FD-Score:5.67|P-value:7.02E-9|Clearance:0.84||SGD DESC:Essential protein involved in rRNA processing and ribosome biogenesis; protein abundance increases in response to DNA replication stress Gene:SHR3(YDL212W)|FD-Score:-3.87|P-value:5.47E-5|Clearance:0||SGD DESC:Endoplasmic reticulum packaging chaperone, required for incorporation of amino acid permeases into COPII coated vesicles for transport to the cell surface Gene:SPB4(YFL002C)|FD-Score:3.7|P-value:1.08E-4|Clearance:0.39||SGD DESC:Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients Gene:TFC3(YAL001C)|FD-Score:4.83|P-value:6.71E-7|Clearance:0.18||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:TFG1(YGR186W)|FD-Score:-3.73|P-value:9.70E-5|Clearance:0||SGD DESC:TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74 Gene:TOM40(YMR203W)|FD-Score:3.13|P-value:8.82E-4|Clearance:0.03||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6801262
Download HOP data (tab-delimited text)  (excel)
Gene:ADH6(YMR318C)|FD-Score:3.82|P-value:6.62E-5||SGD DESC:NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance; protein abundance increases in response to DNA replication stress Gene:AKR1(YDR264C)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:ALF1(YNL148C)|FD-Score:3.85|P-value:5.99E-5||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:COG6(YNL041C)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DIP5(YPL265W)|FD-Score:3.33|P-value:4.41E-4||SGD DESC:Dicarboxylic amino acid permease; mediates high-affinity and high-capacity transport of L-glutamate and L-aspartate; also a transporter for Gln, Asn, Ser, Ala, and Gly; relocalizes from plasma membrane to vacuole upon DNA replication stress Gene:DST1(YGL043W)|FD-Score:4.59|P-value:2.26E-6||SGD DESC:General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress Gene:EAF5(YEL018W)|FD-Score:3.97|P-value:3.59E-5||SGD DESC:Esa1p-associated factor, non-essential subunit of the NuA4 acetyltransferase complex Gene:ERG4(YGL012W)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:ERP3(YDL018C)|FD-Score:-3.54|P-value:2.00E-4||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:FAA3(YIL009W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Long chain fatty acyl-CoA synthetase, activates imported fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:FPR2(YDR519W)|FD-Score:3.92|P-value:4.50E-5||SGD DESC:Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase); binds to the drugs FK506 and rapamycin; expression pattern suggests possible involvement in ER protein trafficking; relocalizes from nucleus to vacuole upon DNA replication stress Gene:GUT1(YHL032C)|FD-Score:-3.99|P-value:3.31E-5||SGD DESC:Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p Gene:HPM1(YIL110W)|FD-Score:3.97|P-value:3.67E-5||SGD DESC:AdoMet-dependent methyltransferase involved in a novel 3-methylhistidine modification of ribosomal protein Rpl3p; seven beta-strand MTase family member; null mutant exhibits a weak vacuolar protein sorting defect and caspofungin resistance Gene:IMA5(YJL216C)|FD-Score:3.99|P-value:3.37E-5||SGD DESC:Alpha-glucosidase; specificity for isomaltose, maltose, and palatinose, but not alpha-methylglucoside; member of the IMA isomaltase family; not required for isomaltose utilization, but Ima5p overexpression allows the ima1 null mutant to grow on isomaltose Gene:IPK1(YDR315C)|FD-Score:3.79|P-value:7.49E-5||SGD DESC:Inositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable Gene:IRC14(YOR135C_d)|FD-Score:-3.12|P-value:8.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Gene:ITR2(YOL103W)|FD-Score:4.44|P-value:4.55E-6||SGD DESC:Myo-inositol transporter with strong similarity to the major myo-inositol transporter Itr1p, member of the sugar transporter superfamily; expressed constitutively Gene:MET13(YGL125W)|FD-Score:4.06|P-value:2.45E-5||SGD DESC:Major isozyme of methylenetetrahydrofolate reductase, catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the methionine biosynthesis pathway Gene:MMM1(YLL006W)|FD-Score:6.18|P-value:3.23E-10||SGD DESC:ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MRK1(YDL079C)|FD-Score:3.95|P-value:3.90E-5||SGD DESC:Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation Gene:MRPL38(YKL170W)|FD-Score:5.2|P-value:9.90E-8||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress Gene:MRPL40(YPL173W)|FD-Score:4.04|P-value:2.66E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSF1(YPR047W)|FD-Score:3.97|P-value:3.54E-5||SGD DESC:Mitochondrial phenylalanyl-tRNA synthetase, active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase Gene:MSW1(YDR268W)|FD-Score:-3.86|P-value:5.65E-5||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:NPR3(YHL023C)|FD-Score:4.13|P-value:1.82E-5||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; required for Npr2p phosphorylation and Iml1p-Npr2p interaction; null mutant shows delayed meiotic DNA replication and double-strand break repair Gene:PEP3(YLR148W)|FD-Score:3.19|P-value:7.00E-4||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PET112(YBL080C)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog Gene:PIG1(YLR273C)|FD-Score:-3.56|P-value:1.89E-4||SGD DESC:Putative targeting subunit for the type-1 protein phosphatase Glc7p that tethers it to the Gsy2p glycogen synthase Gene:PRM2(YIL037C)|FD-Score:3.87|P-value:5.51E-5||SGD DESC:Pheromone-regulated protein, predicted to have 4 transmembrane segments and a coiled coil domain; regulated by Ste12p; required for efficient nuclear fusion Gene:PRO2(YOR323C)|FD-Score:8.49|P-value:1.08E-17||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PRS3(YHL011C)|FD-Score:3.78|P-value:7.95E-5||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:RPL24A(YGL031C)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Ribosomal 60S subunit protein L24A; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24A has a paralog, RPL24B, that arose from the whole genome duplication Gene:RPP1A(YDL081C)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Ribosomal stalk protein P1 alpha, involved in the interaction between translational elongation factors and the ribosome; accumulation of P1 in the cytoplasm is regulated by phosphorylation and interaction with the P2 stalk component Gene:RPS14B(YJL191W)|FD-Score:3.98|P-value:3.41E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14B has a paralog, RPS14A, that arose from the whole genome duplication Gene:RRG1(YDR065W)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Protein of unknown function, required for vacuolar acidification and mitochondrial genome maintenance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RTF1(YGL244W)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:SBH1(YER087C-B)|FD-Score:3.27|P-value:5.30E-4||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SKI2(YLR398C)|FD-Score:3.73|P-value:9.71E-5||SGD DESC:Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SKY1(YMR216C)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SPO73(YER046W)|FD-Score:-4.08|P-value:2.25E-5||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensible for both nuclear divisions during meiosis Gene:SPR1(YOR190W)|FD-Score:3.92|P-value:4.46E-5||SGD DESC:Sporulation-specific exo-1,3-beta-glucanase; contributes to ascospore thermoresistance Gene:SPR6(YER115C)|FD-Score:3.21|P-value:6.68E-4||SGD DESC:Protein of unknown function, expressed during sporulation; not required for sporulation, but gene exhibits genetic interactions with other genes required for sporulation Gene:SPT10(YJL127C)|FD-Score:4.7|P-value:1.30E-6||SGD DESC:Putative histone acetylase with a role in transcriptional silencing, sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box Gene:VPH1(YOR270C)|FD-Score:3.11|P-value:9.33E-4||SGD DESC:Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress Gene:VPS53(YJL029C)|FD-Score:-3.4|P-value:3.34E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; required for vacuolar protein sorting Gene:YAL056C-A(YAL056C-A_d)|FD-Score:4.81|P-value:7.39E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR219C(YBR219C_p)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Putative protein of unknown function; YBR219C is not an essential gene Gene:YFL032W(YFL032W_d)|FD-Score:3.17|P-value:7.65E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene Gene:YIL012W(YIL012W_d)|FD-Score:5.13|P-value:1.45E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKR041W(YKR041W)|FD-Score:-4.01|P-value:3.02E-5||SGD DESC:Protein of unknown function; localizes to the mitotic spindle; overexpression of YKR041W affects endocytic protein trafficking Gene:YLR312C(YLR312C_p)|FD-Score:3.17|P-value:7.67E-4||SGD DESC:Putative protein of unknown function Gene:YMR082C(YMR082C_d)|FD-Score:4.49|P-value:3.57E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR315W(YMR315W)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Protein with NADP(H) oxidoreductase activity; transcription is regulated by Stb5p in response to NADPH depletion induced by diamide; promoter contains a putative Stb5p binding site; protein abundance increases in response to DNA replication stress Gene:YNL195C(YNL195C_p)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPK1(YKL126W)|FD-Score:3.89|P-value:4.99E-5||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR071W(YPR071W_p)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Putative membrane protein; YPR071W is not an essential gene; YPR071W has paralog YIL029C Gene:YPR197C(YPR197C_d)|FD-Score:4.55|P-value:2.74E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPT35(YHR105W)|FD-Score:-3.6|P-value:1.58E-4||SGD DESC:Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport Gene:ADH6(YMR318C)|FD-Score:3.82|P-value:6.62E-5||SGD DESC:NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance; protein abundance increases in response to DNA replication stress Gene:AKR1(YDR264C)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:ALF1(YNL148C)|FD-Score:3.85|P-value:5.99E-5||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:COG6(YNL041C)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DIP5(YPL265W)|FD-Score:3.33|P-value:4.41E-4||SGD DESC:Dicarboxylic amino acid permease; mediates high-affinity and high-capacity transport of L-glutamate and L-aspartate; also a transporter for Gln, Asn, Ser, Ala, and Gly; relocalizes from plasma membrane to vacuole upon DNA replication stress Gene:DST1(YGL043W)|FD-Score:4.59|P-value:2.26E-6||SGD DESC:General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress Gene:EAF5(YEL018W)|FD-Score:3.97|P-value:3.59E-5||SGD DESC:Esa1p-associated factor, non-essential subunit of the NuA4 acetyltransferase complex Gene:ERG4(YGL012W)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:ERP3(YDL018C)|FD-Score:-3.54|P-value:2.00E-4||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:FAA3(YIL009W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Long chain fatty acyl-CoA synthetase, activates imported fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:FPR2(YDR519W)|FD-Score:3.92|P-value:4.50E-5||SGD DESC:Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase); binds to the drugs FK506 and rapamycin; expression pattern suggests possible involvement in ER protein trafficking; relocalizes from nucleus to vacuole upon DNA replication stress Gene:GUT1(YHL032C)|FD-Score:-3.99|P-value:3.31E-5||SGD DESC:Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p Gene:HPM1(YIL110W)|FD-Score:3.97|P-value:3.67E-5||SGD DESC:AdoMet-dependent methyltransferase involved in a novel 3-methylhistidine modification of ribosomal protein Rpl3p; seven beta-strand MTase family member; null mutant exhibits a weak vacuolar protein sorting defect and caspofungin resistance Gene:IMA5(YJL216C)|FD-Score:3.99|P-value:3.37E-5||SGD DESC:Alpha-glucosidase; specificity for isomaltose, maltose, and palatinose, but not alpha-methylglucoside; member of the IMA isomaltase family; not required for isomaltose utilization, but Ima5p overexpression allows the ima1 null mutant to grow on isomaltose Gene:IPK1(YDR315C)|FD-Score:3.79|P-value:7.49E-5||SGD DESC:Inositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable Gene:IRC14(YOR135C_d)|FD-Score:-3.12|P-value:8.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Gene:ITR2(YOL103W)|FD-Score:4.44|P-value:4.55E-6||SGD DESC:Myo-inositol transporter with strong similarity to the major myo-inositol transporter Itr1p, member of the sugar transporter superfamily; expressed constitutively Gene:MET13(YGL125W)|FD-Score:4.06|P-value:2.45E-5||SGD DESC:Major isozyme of methylenetetrahydrofolate reductase, catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the methionine biosynthesis pathway Gene:MMM1(YLL006W)|FD-Score:6.18|P-value:3.23E-10||SGD DESC:ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MRK1(YDL079C)|FD-Score:3.95|P-value:3.90E-5||SGD DESC:Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation Gene:MRPL38(YKL170W)|FD-Score:5.2|P-value:9.90E-8||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress Gene:MRPL40(YPL173W)|FD-Score:4.04|P-value:2.66E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSF1(YPR047W)|FD-Score:3.97|P-value:3.54E-5||SGD DESC:Mitochondrial phenylalanyl-tRNA synthetase, active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase Gene:MSW1(YDR268W)|FD-Score:-3.86|P-value:5.65E-5||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:NPR3(YHL023C)|FD-Score:4.13|P-value:1.82E-5||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; required for Npr2p phosphorylation and Iml1p-Npr2p interaction; null mutant shows delayed meiotic DNA replication and double-strand break repair Gene:PEP3(YLR148W)|FD-Score:3.19|P-value:7.00E-4||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PET112(YBL080C)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog Gene:PIG1(YLR273C)|FD-Score:-3.56|P-value:1.89E-4||SGD DESC:Putative targeting subunit for the type-1 protein phosphatase Glc7p that tethers it to the Gsy2p glycogen synthase Gene:PRM2(YIL037C)|FD-Score:3.87|P-value:5.51E-5||SGD DESC:Pheromone-regulated protein, predicted to have 4 transmembrane segments and a coiled coil domain; regulated by Ste12p; required for efficient nuclear fusion Gene:PRO2(YOR323C)|FD-Score:8.49|P-value:1.08E-17||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PRS3(YHL011C)|FD-Score:3.78|P-value:7.95E-5||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:RPL24A(YGL031C)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Ribosomal 60S subunit protein L24A; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24A has a paralog, RPL24B, that arose from the whole genome duplication Gene:RPP1A(YDL081C)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Ribosomal stalk protein P1 alpha, involved in the interaction between translational elongation factors and the ribosome; accumulation of P1 in the cytoplasm is regulated by phosphorylation and interaction with the P2 stalk component Gene:RPS14B(YJL191W)|FD-Score:3.98|P-value:3.41E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14B has a paralog, RPS14A, that arose from the whole genome duplication Gene:RRG1(YDR065W)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Protein of unknown function, required for vacuolar acidification and mitochondrial genome maintenance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RTF1(YGL244W)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:SBH1(YER087C-B)|FD-Score:3.27|P-value:5.30E-4||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SKI2(YLR398C)|FD-Score:3.73|P-value:9.71E-5||SGD DESC:Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SKY1(YMR216C)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SPO73(YER046W)|FD-Score:-4.08|P-value:2.25E-5||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensible for both nuclear divisions during meiosis Gene:SPR1(YOR190W)|FD-Score:3.92|P-value:4.46E-5||SGD DESC:Sporulation-specific exo-1,3-beta-glucanase; contributes to ascospore thermoresistance Gene:SPR6(YER115C)|FD-Score:3.21|P-value:6.68E-4||SGD DESC:Protein of unknown function, expressed during sporulation; not required for sporulation, but gene exhibits genetic interactions with other genes required for sporulation Gene:SPT10(YJL127C)|FD-Score:4.7|P-value:1.30E-6||SGD DESC:Putative histone acetylase with a role in transcriptional silencing, sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box Gene:VPH1(YOR270C)|FD-Score:3.11|P-value:9.33E-4||SGD DESC:Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress Gene:VPS53(YJL029C)|FD-Score:-3.4|P-value:3.34E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; required for vacuolar protein sorting Gene:YAL056C-A(YAL056C-A_d)|FD-Score:4.81|P-value:7.39E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR219C(YBR219C_p)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Putative protein of unknown function; YBR219C is not an essential gene Gene:YFL032W(YFL032W_d)|FD-Score:3.17|P-value:7.65E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene Gene:YIL012W(YIL012W_d)|FD-Score:5.13|P-value:1.45E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKR041W(YKR041W)|FD-Score:-4.01|P-value:3.02E-5||SGD DESC:Protein of unknown function; localizes to the mitotic spindle; overexpression of YKR041W affects endocytic protein trafficking Gene:YLR312C(YLR312C_p)|FD-Score:3.17|P-value:7.67E-4||SGD DESC:Putative protein of unknown function Gene:YMR082C(YMR082C_d)|FD-Score:4.49|P-value:3.57E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR315W(YMR315W)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Protein with NADP(H) oxidoreductase activity; transcription is regulated by Stb5p in response to NADPH depletion induced by diamide; promoter contains a putative Stb5p binding site; protein abundance increases in response to DNA replication stress Gene:YNL195C(YNL195C_p)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPK1(YKL126W)|FD-Score:3.89|P-value:4.99E-5||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR071W(YPR071W_p)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Putative membrane protein; YPR071W is not an essential gene; YPR071W has paralog YIL029C Gene:YPR197C(YPR197C_d)|FD-Score:4.55|P-value:2.74E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPT35(YHR105W)|FD-Score:-3.6|P-value:1.58E-4||SGD DESC:Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YCL031C5.677.02E-90.84RRP7Essential protein involved in rRNA processing and ribosome biogenesis; protein abundance increases in response to DNA replication stress
YAL001C4.836.71E-70.18TFC3Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding
YDL150W4.651.63E-60.20RPC53RNA polymerase III subunit C53
YJL010C4.464.12E-60.09NOP9Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain
YBL074C4.376.28E-60.46AAR2Component of the U5 snRNP, required for splicing of U3 precursors; originally described as a splicing factor specifically required for splicing pre-mRNA of the MATa1 cistron
YJL011C3.914.64E-50.00RPC17RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress
YJR093C3.914.67E-50.20FIP1Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p
YKL078W3.711.03E-40.01DHR2Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis
YFL002C3.701.08E-40.39SPB4Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients
YOR160W3.314.64E-40.12MTR10Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
YBR142W3.197.06E-40.00MAK5Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits
YER165W3.197.16E-40.06PAB1Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G
YBL018C3.138.77E-40.00POP8Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YMR203W3.138.82E-40.03TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YGR145W3.109.77E-40.12ENP2Essential nucleolar protein, required for biogenesis of the small ribosomal subunit; contains WD repeats, interacts with Mpp10p and Bfr2p, and has homology to Spb1p

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOR323C8.491.08E-17PRO2Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis
YLL006W6.183.23E-10MMM1ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth
YKL170W5.209.90E-8MRPL38Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress
YIL012W_d5.131.45E-7YIL012W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YAL056C-A_d4.817.39E-7YAL056C-A_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YJL127C4.701.30E-6SPT10Putative histone acetylase with a role in transcriptional silencing, sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box
YGL043W4.592.26E-6DST1General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress
YPR197C_d4.552.74E-6YPR197C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YMR082C_d4.493.57E-6YMR082C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YOL103W4.444.55E-6ITR2Myo-inositol transporter with strong similarity to the major myo-inositol transporter Itr1p, member of the sugar transporter superfamily; expressed constitutively
YHL023C4.131.82E-5NPR3Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; required for Npr2p phosphorylation and Iml1p-Npr2p interaction; null mutant shows delayed meiotic DNA replication and double-strand break repair
YGL125W4.062.45E-5MET13Major isozyme of methylenetetrahydrofolate reductase, catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the methionine biosynthesis pathway
YPL173W4.042.66E-5MRPL40Mitochondrial ribosomal protein of the large subunit
YJL216C3.993.37E-5IMA5Alpha-glucosidase; specificity for isomaltose, maltose, and palatinose, but not alpha-methylglucoside; member of the IMA isomaltase family; not required for isomaltose utilization, but Ima5p overexpression allows the ima1 null mutant to grow on isomaltose
YJL191W3.983.41E-5RPS14BProtein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14B has a paralog, RPS14A, that arose from the whole genome duplication

GO enrichment analysis for SGTC_1018
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1086.96E-17SGTC_1973st070275 50.2 μMTimTec (Natural product derivative library)171183340.0789474
0.1055.97E-16SGTC_772k072-0172 35.3 μMChemDiv (Drug-like library)58619800.0641026
0.1031.70E-15SGTC_7710250-0039 27.3 μMChemDiv (Drug-like library)30051150.0533333
0.1001.36E-14SGTC_2501sodium tetradecyl sulfate 13.4 μMMicrosource (Natural product library)236657720.027027
0.0961.58E-13SGTC_10114112-3381 17.4 μMChemDiv (Drug-like library)28771390.126582
0.0946.00E-13SGTC_14224031-0024 15.5 μMChemDiv (Drug-like library)7824270.08iron homeostasis
0.0904.55E-12SGTC_22467293507 91.6 μMChembridge (Fragment library)8876560.0714286
0.0905.24E-12SGTC_13772944-0135 198.0 μMChemDiv (Drug-like library)18941370.0963855calcium & mitochondrial duress
0.0881.29E-11SGTC_23327982454 138.9 μMChembridge (Fragment library)29817480.0649351
0.0863.87E-11SGTC_31059121040 49.5 μMChembridge (Drug-like library)414489070.0649351
0.0855.64E-11SGTC_468gf-109203x 121.0 μMICCB bioactive library23960.0697674amide catabolism
0.0791.51E-9SGTC_6604031-1742 102.0 μMChemDiv (Drug-like library)67387060.0963855redox potentiating
0.0791.54E-9SGTC_32379133362 49.5 μMChembridge (Drug-like library)438451370.104651
0.0782.39E-9SGTC_20415107442 45.3 μMChembridge (Fragment library)7930050.078125RSC complex & mRNA processing
0.0765.47E-9SGTC_10543429-0080 167.0 μMChemDiv (Drug-like library)11628770.0853659

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2780521529871.43 μM0.3225812834131Chembridge (Drug-like library)320.341942.32915
SGTC_2713st07914953.4 μM0.301373103253TimTec (Natural product derivative library)374.430842.91635
SGTC_3076911618749.47 μM0.29687527241210Chembridge (Drug-like library)327.783263.20925
SGTC_328k915-012063.5 μM0.2941186624543ChemDiv (Drug-like library)388.458964.48825TSC3-RPN4
SGTC_2669eugenol100 μM0.2909093314Microsource (Natural product library)164.201082.57912
SGTC_315k915-0449187 μM0.28947420970215ChemDiv (Drug-like library)465.38226.19524
SGTC_3041310-029897 μM0.2833331550234ChemDiv (Drug-like library)366.407084.6225cell wall
SGTC_3084092-1169104 μM0.2656252268590ChemDiv (Drug-like library)346.851123.8514plasma membrane duress
SGTC_368fentichlor4.58 μM0.267329ChemDiv (Drug-like library)287.161724.79323
SGTC_1773st04854273.58 μM0.2539683237138TimTec (Natural product derivative library)251.344582.90214