4476-0760

(E)-3-[3-(4-chlorophenyl)-1-phenylpyrazol-4-yl]prop-2-enoic acid

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1021
Screen concentration 85.3 μM
Source ChemDiv (Drug-like library)
PubChem CID 746768
SMILES C1=CC=C(C=C1)N2C=C(C(=N2)C3=CC=C(C=C3)Cl)C=CC(=O)O
Standardized SMILES OC(=O)C=Cc1cn(nc1c2ccc(Cl)cc2)c3ccccc3
Molecular weight 324.761
ALogP 4.77
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.3
% growth inhibition (Hom. pool) 3.91


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 746768
Download HIP data (tab-delimited text)  (excel)
Gene:HIP1(YGR191W)|FD-Score:3.42|P-value:3.09E-4|Clearance:0.17||SGD DESC:High-affinity histidine permease, also involved in the transport of manganese ions Gene:IPL1(YPL209C)|FD-Score:3.21|P-value:6.55E-4|Clearance:0.12||SGD DESC:Aurora kinase subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), involved in regulating kinetochore-microtubule attachments; helps maintain condensed chromosomes during anaphase and early telophase; required for SPB cohesion and prevention of multipolar spindle formation; localizes to nuclear foci that become diffuse upon DNA replication stress; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon SAC arrest Gene:IRR1(YIL026C)|FD-Score:4.88|P-value:5.36E-7|Clearance:1.13||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:MCM10(YIL150C)|FD-Score:3.43|P-value:3.04E-4|Clearance:0||SGD DESC:Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins Gene:POL3(YDL102W)|FD-Score:-3.98|P-value:3.50E-5|Clearance:0||SGD DESC:Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER) Gene:PRE6(YOL038W)|FD-Score:3.25|P-value:5.69E-4|Clearance:0.04||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:RIB5(YBR256C)|FD-Score:3.1|P-value:9.76E-4|Clearance:0.06||SGD DESC:Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway Gene:YPL251W(YPL251W_d)|FD-Score:4.56|P-value:2.56E-6|Clearance:1.13||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C Gene:HIP1(YGR191W)|FD-Score:3.42|P-value:3.09E-4|Clearance:0.17||SGD DESC:High-affinity histidine permease, also involved in the transport of manganese ions Gene:IPL1(YPL209C)|FD-Score:3.21|P-value:6.55E-4|Clearance:0.12||SGD DESC:Aurora kinase subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), involved in regulating kinetochore-microtubule attachments; helps maintain condensed chromosomes during anaphase and early telophase; required for SPB cohesion and prevention of multipolar spindle formation; localizes to nuclear foci that become diffuse upon DNA replication stress; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon SAC arrest Gene:IRR1(YIL026C)|FD-Score:4.88|P-value:5.36E-7|Clearance:1.13||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:MCM10(YIL150C)|FD-Score:3.43|P-value:3.04E-4|Clearance:0||SGD DESC:Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins Gene:POL3(YDL102W)|FD-Score:-3.98|P-value:3.50E-5|Clearance:0||SGD DESC:Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER) Gene:PRE6(YOL038W)|FD-Score:3.25|P-value:5.69E-4|Clearance:0.04||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:RIB5(YBR256C)|FD-Score:3.1|P-value:9.76E-4|Clearance:0.06||SGD DESC:Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway Gene:YPL251W(YPL251W_d)|FD-Score:4.56|P-value:2.56E-6|Clearance:1.13||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 746768
Download HOP data (tab-delimited text)  (excel)
Gene:ADE1(YAR015W)|FD-Score:-3.09|P-value:0.00100||SGD DESC:N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress Gene:APE3(YBR286W)|FD-Score:3.92|P-value:4.34E-5||SGD DESC:Vacuolar aminopeptidase Y, processed to mature form by Prb1p Gene:APN1(YKL114C)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:ATG8(YBL078C)|FD-Score:3.12|P-value:9.06E-4||SGD DESC:Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis Gene:BDS1(YOL164W)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Bacterially-derived sulfatase required for use of alkyl- and aryl-sulfates as sulfur sources Gene:BIT2(YBR270C_p)|FD-Score:3.83|P-value:6.40E-5||SGD DESC:Subunit of TORC2 membrane-associated complex; involved in regulation of actin cytoskeletal dynamics during polarized growth and cell wall integrity; interacts with Slm1p and Slm2p, homologous PH domain-containing TORC2 substrates; BIT2 has a paralog, BIT61, that arose from the whole genome duplication Gene:BNA1(YJR025C)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:3-hydroxyanthranilic acid dioxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:BNA4(YBL098W)|FD-Score:3.24|P-value:5.91E-4||SGD DESC:Kynurenine 3-mono oxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p; putative therapeutic target for Huntington disease Gene:BNI4(YNL233W)|FD-Score:3.97|P-value:3.55E-5||SGD DESC:Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p Gene:BST1(YFL025C)|FD-Score:3.79|P-value:7.61E-5||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:CCR4(YAL021C)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:COG7(YGL005C)|FD-Score:4.03|P-value:2.81E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DSF2(YBR007C_p)|FD-Score:8.23|P-value:9.38E-17||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ECM19(YLR390W)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FYV5(YCL058C)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Protein involved in regulation of the mating pathway; binds with Matalpha2p to promoters of haploid-specific genes; required for survival upon exposure to K1 killer toxin; involved in ion homeostasis Gene:HPR1(YDR138W)|FD-Score:5.36|P-value:4.10E-8||SGD DESC:Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p Gene:HXK1(YFR053C)|FD-Score:-3.13|P-value:8.63E-4||SGD DESC:Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication Gene:IST1(YNL265C)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Protein with a positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p Gene:KTR7(YIL085C)|FD-Score:-3.15|P-value:8.20E-4||SGD DESC:Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR7 has a paralog, KTR5, that arose from the whole genome duplication Gene:LDB19(YOR322C)|FD-Score:5.19|P-value:1.05E-7||SGD DESC:Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye Gene:LYS1(YIR034C)|FD-Score:6.2|P-value:2.81E-10||SGD DESC:Saccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; also has mRNA binding activity Gene:MOH1(YBL049W)|FD-Score:4.71|P-value:1.23E-6||SGD DESC:Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase Gene:MRP51(YPL118W)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:MRPL16(YBL038W)|FD-Score:-3.92|P-value:4.36E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSO1(YNR049C)|FD-Score:-3.7|P-value:1.06E-4||SGD DESC:Probable component of the secretory vesicle docking complex; acts at a late step in secretion; shows genetic and physical interactions with Sec1p; required for prospore membrane formation during sporulation; relocalizes from bud neck to nucleus upon DNA replication stress Gene:NIP100(YPL174C)|FD-Score:5.57|P-value:1.29E-8||SGD DESC:Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) Gene:OPT2(YPR194C)|FD-Score:3.78|P-value:7.78E-5||SGD DESC:Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles Gene:PAA1(YDR071C)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication Gene:PAF1(YBR279W)|FD-Score:5.63|P-value:9.00E-9||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 Gene:PDH1(YPR002W)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Mitochondrial protein that participates in respiration, induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate Gene:PGD1(YGL025C)|FD-Score:4.58|P-value:2.29E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PIG2(YIL045W)|FD-Score:3.93|P-value:4.20E-5||SGD DESC:Putative type-1 protein phosphatase targeting subunit that tethers Glc7p type-1 protein phosphatase to Gsy2p glycogen synthase Gene:PIL1(YGR086C)|FD-Score:4.06|P-value:2.49E-5||SGD DESC:Primary protein component of eisosomes; eisosomes are large immobile cell cortex structures associated with endocytosis; null mutant shows activation of Pkc1p/Ypk1p stress resistance pathways; detected in phosphorylated state in mitochondria; member of the BAR domain family; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:PIN3(YPR154W)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced Gene:PPA2(YMR267W)|FD-Score:-3.51|P-value:2.22E-4||SGD DESC:Mitochondrial inorganic pyrophosphatase, required for mitochondrial function and possibly involved in energy generation from inorganic pyrophosphate Gene:PPT2(YPL148C)|FD-Score:3.77|P-value:8.31E-5||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PST2(YDR032C)|FD-Score:4.41|P-value:5.16E-6||SGD DESC:Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication Gene:RAM1(YDL090C)|FD-Score:3.19|P-value:7.04E-4||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RPL16B(YNL069C)|FD-Score:3.73|P-value:9.50E-5||SGD DESC:Ribosomal 60S subunit protein L16B; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16B has a paralog, RPL16A, that arose from the whole genome duplication Gene:RPL22A(YLR061W)|FD-Score:6.8|P-value:5.18E-12||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RPL36B(YPL249C-A)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Ribosomal 60S subunit protein L36B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36B has a paralog, RPL36A, that arose from the whole genome duplication Gene:RPL37B(YDR500C)|FD-Score:5.03|P-value:2.48E-7||SGD DESC:Ribosomal 60S subunit protein L37B; protein abundance increases in response to DNA replication stress; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37B has a paralog, RPL37A, that arose from the whole genome duplication Gene:RPL41B(YDL133C-A)|FD-Score:-3.13|P-value:8.82E-4||SGD DESC:Ribosomal 60S subunit protein L41B; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41B has a paralog, RPL41A, that arose from the whole genome duplication Gene:RPS1B(YML063W)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1B has a paralog, RPS1A, that arose from the whole genome duplication Gene:RRG7(YOR305W)|FD-Score:3.09|P-value:9.85E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YOR305W is not an essential gene Gene:SCM4(YGR049W)|FD-Score:4.14|P-value:1.74E-5||SGD DESC:Mitochondrial outer membrane protein of unknown function; predicted to have 4 transmembrane segments; import is mediated by Tom70p and Mim1p; interacts genetically with a cdc4 mutation Gene:SIS2(YKR072C)|FD-Score:4.52|P-value:3.15E-6||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SLX8(YER116C)|FD-Score:4.59|P-value:2.18E-6||SGD DESC:Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachment of SUMO to the substrate; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress Gene:STM1(YLR150W)|FD-Score:3.16|P-value:7.93E-4||SGD DESC:Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery Gene:URA8(YJR103W)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Minor CTP synthase isozyme (see also URA7); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA8 has a paralog, URA7, that arose from the whole genome duplication Gene:URM1(YIL008W)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:VPS8(YAL002W)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif Gene:WSC2(YNL283C)|FD-Score:4.18|P-value:1.47E-5||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response Gene:YBR016W(YBR016W)|FD-Score:3.95|P-value:3.91E-5||SGD DESC:Tail-anchored plasma membrane protein with a conserved CYSTM module; predicted to be palmitoylated; has similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions; YBR016W has a paralog, YDL012C, that arose from the whole genome duplication Gene:YBR238C(YBR238C)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YBR284W(YBR284W_p)|FD-Score:3.95|P-value:3.84E-5||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication Gene:YBR300C(YBR300C_d)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR301W; YBR300C is not an essential gene Gene:YDL094C(YDL094C_d)|FD-Score:3.29|P-value:5.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verfied gene PMT5/YDL093W; YDL094C is not essential Gene:YDR179W-A(YDR179W-A_p)|FD-Score:3.29|P-value:4.98E-4||SGD DESC:Putative protein of unknown function Gene:YEL014C(YEL014C_d)|FD-Score:4|P-value:3.21E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YEL025C(YEL025C_p)|FD-Score:-3.56|P-value:1.86E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YER034W(YER034W_p)|FD-Score:3.86|P-value:5.66E-5||SGD DESC:Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress Gene:YGP1(YNL160W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Cell wall-related secretory glycoprotein; induced by nutrient deprivation-associated growth arrest and upon entry into stationary phase; may be involved in adaptation prior to stationary phase entry; has similarity to Sps100p Gene:YHL008C(YHL008C_p)|FD-Score:4.77|P-value:9.16E-7||SGD DESC:Putative protein of unknown function, may be involved in the uptake of chloride ions; does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YHR097C(YHR097C_p)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus Gene:YIL025C(YIL025C_d)|FD-Score:-3.14|P-value:8.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL069W(YKL069W)|FD-Score:3.88|P-value:5.26E-5||SGD DESC:Methionine-R-sulfoxide reductase; reduces the R enantiomer of free Met-SO, in contrast to Ycl033Cp which reduces Met-R-SO in a peptide linkage; has a role in protection against oxidative stress; relative distribution to the nucleus increases upon DNA replication stress Gene:YNL190W(YNL190W_p)|FD-Score:6.19|P-value:3.02E-10||SGD DESC:Hydrophilin essential in dessication-rehydration process; cell wall protein; contains a putative GPI-attachment site Gene:YNL195C(YNL195C_p)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOR114W(YOR114W_p)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Putative protein of unknown function; null mutant is viable Gene:YOR366W(YOR366W_d)|FD-Score:4.19|P-value:1.42E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C Gene:YPR108W-A(YPR108W-A_p)|FD-Score:3.94|P-value:4.06E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPR127W(YPR127W)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Putative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:ADE1(YAR015W)|FD-Score:-3.09|P-value:0.00100||SGD DESC:N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress Gene:APE3(YBR286W)|FD-Score:3.92|P-value:4.34E-5||SGD DESC:Vacuolar aminopeptidase Y, processed to mature form by Prb1p Gene:APN1(YKL114C)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:ATG8(YBL078C)|FD-Score:3.12|P-value:9.06E-4||SGD DESC:Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis Gene:BDS1(YOL164W)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Bacterially-derived sulfatase required for use of alkyl- and aryl-sulfates as sulfur sources Gene:BIT2(YBR270C_p)|FD-Score:3.83|P-value:6.40E-5||SGD DESC:Subunit of TORC2 membrane-associated complex; involved in regulation of actin cytoskeletal dynamics during polarized growth and cell wall integrity; interacts with Slm1p and Slm2p, homologous PH domain-containing TORC2 substrates; BIT2 has a paralog, BIT61, that arose from the whole genome duplication Gene:BNA1(YJR025C)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:3-hydroxyanthranilic acid dioxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:BNA4(YBL098W)|FD-Score:3.24|P-value:5.91E-4||SGD DESC:Kynurenine 3-mono oxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p; putative therapeutic target for Huntington disease Gene:BNI4(YNL233W)|FD-Score:3.97|P-value:3.55E-5||SGD DESC:Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p Gene:BST1(YFL025C)|FD-Score:3.79|P-value:7.61E-5||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:CCR4(YAL021C)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:COG7(YGL005C)|FD-Score:4.03|P-value:2.81E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DSF2(YBR007C_p)|FD-Score:8.23|P-value:9.38E-17||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ECM19(YLR390W)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FYV5(YCL058C)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Protein involved in regulation of the mating pathway; binds with Matalpha2p to promoters of haploid-specific genes; required for survival upon exposure to K1 killer toxin; involved in ion homeostasis Gene:HPR1(YDR138W)|FD-Score:5.36|P-value:4.10E-8||SGD DESC:Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p Gene:HXK1(YFR053C)|FD-Score:-3.13|P-value:8.63E-4||SGD DESC:Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication Gene:IST1(YNL265C)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Protein with a positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p Gene:KTR7(YIL085C)|FD-Score:-3.15|P-value:8.20E-4||SGD DESC:Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR7 has a paralog, KTR5, that arose from the whole genome duplication Gene:LDB19(YOR322C)|FD-Score:5.19|P-value:1.05E-7||SGD DESC:Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye Gene:LYS1(YIR034C)|FD-Score:6.2|P-value:2.81E-10||SGD DESC:Saccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; also has mRNA binding activity Gene:MOH1(YBL049W)|FD-Score:4.71|P-value:1.23E-6||SGD DESC:Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase Gene:MRP51(YPL118W)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:MRPL16(YBL038W)|FD-Score:-3.92|P-value:4.36E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSO1(YNR049C)|FD-Score:-3.7|P-value:1.06E-4||SGD DESC:Probable component of the secretory vesicle docking complex; acts at a late step in secretion; shows genetic and physical interactions with Sec1p; required for prospore membrane formation during sporulation; relocalizes from bud neck to nucleus upon DNA replication stress Gene:NIP100(YPL174C)|FD-Score:5.57|P-value:1.29E-8||SGD DESC:Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) Gene:OPT2(YPR194C)|FD-Score:3.78|P-value:7.78E-5||SGD DESC:Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles Gene:PAA1(YDR071C)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication Gene:PAF1(YBR279W)|FD-Score:5.63|P-value:9.00E-9||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 Gene:PDH1(YPR002W)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Mitochondrial protein that participates in respiration, induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate Gene:PGD1(YGL025C)|FD-Score:4.58|P-value:2.29E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PIG2(YIL045W)|FD-Score:3.93|P-value:4.20E-5||SGD DESC:Putative type-1 protein phosphatase targeting subunit that tethers Glc7p type-1 protein phosphatase to Gsy2p glycogen synthase Gene:PIL1(YGR086C)|FD-Score:4.06|P-value:2.49E-5||SGD DESC:Primary protein component of eisosomes; eisosomes are large immobile cell cortex structures associated with endocytosis; null mutant shows activation of Pkc1p/Ypk1p stress resistance pathways; detected in phosphorylated state in mitochondria; member of the BAR domain family; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:PIN3(YPR154W)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced Gene:PPA2(YMR267W)|FD-Score:-3.51|P-value:2.22E-4||SGD DESC:Mitochondrial inorganic pyrophosphatase, required for mitochondrial function and possibly involved in energy generation from inorganic pyrophosphate Gene:PPT2(YPL148C)|FD-Score:3.77|P-value:8.31E-5||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PST2(YDR032C)|FD-Score:4.41|P-value:5.16E-6||SGD DESC:Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication Gene:RAM1(YDL090C)|FD-Score:3.19|P-value:7.04E-4||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RPL16B(YNL069C)|FD-Score:3.73|P-value:9.50E-5||SGD DESC:Ribosomal 60S subunit protein L16B; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16B has a paralog, RPL16A, that arose from the whole genome duplication Gene:RPL22A(YLR061W)|FD-Score:6.8|P-value:5.18E-12||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RPL36B(YPL249C-A)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Ribosomal 60S subunit protein L36B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36B has a paralog, RPL36A, that arose from the whole genome duplication Gene:RPL37B(YDR500C)|FD-Score:5.03|P-value:2.48E-7||SGD DESC:Ribosomal 60S subunit protein L37B; protein abundance increases in response to DNA replication stress; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37B has a paralog, RPL37A, that arose from the whole genome duplication Gene:RPL41B(YDL133C-A)|FD-Score:-3.13|P-value:8.82E-4||SGD DESC:Ribosomal 60S subunit protein L41B; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41B has a paralog, RPL41A, that arose from the whole genome duplication Gene:RPS1B(YML063W)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1B has a paralog, RPS1A, that arose from the whole genome duplication Gene:RRG7(YOR305W)|FD-Score:3.09|P-value:9.85E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YOR305W is not an essential gene Gene:SCM4(YGR049W)|FD-Score:4.14|P-value:1.74E-5||SGD DESC:Mitochondrial outer membrane protein of unknown function; predicted to have 4 transmembrane segments; import is mediated by Tom70p and Mim1p; interacts genetically with a cdc4 mutation Gene:SIS2(YKR072C)|FD-Score:4.52|P-value:3.15E-6||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SLX8(YER116C)|FD-Score:4.59|P-value:2.18E-6||SGD DESC:Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachment of SUMO to the substrate; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress Gene:STM1(YLR150W)|FD-Score:3.16|P-value:7.93E-4||SGD DESC:Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery Gene:URA8(YJR103W)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Minor CTP synthase isozyme (see also URA7); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA8 has a paralog, URA7, that arose from the whole genome duplication Gene:URM1(YIL008W)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:VPS8(YAL002W)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif Gene:WSC2(YNL283C)|FD-Score:4.18|P-value:1.47E-5||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response Gene:YBR016W(YBR016W)|FD-Score:3.95|P-value:3.91E-5||SGD DESC:Tail-anchored plasma membrane protein with a conserved CYSTM module; predicted to be palmitoylated; has similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions; YBR016W has a paralog, YDL012C, that arose from the whole genome duplication Gene:YBR238C(YBR238C)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YBR284W(YBR284W_p)|FD-Score:3.95|P-value:3.84E-5||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication Gene:YBR300C(YBR300C_d)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR301W; YBR300C is not an essential gene Gene:YDL094C(YDL094C_d)|FD-Score:3.29|P-value:5.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verfied gene PMT5/YDL093W; YDL094C is not essential Gene:YDR179W-A(YDR179W-A_p)|FD-Score:3.29|P-value:4.98E-4||SGD DESC:Putative protein of unknown function Gene:YEL014C(YEL014C_d)|FD-Score:4|P-value:3.21E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YEL025C(YEL025C_p)|FD-Score:-3.56|P-value:1.86E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YER034W(YER034W_p)|FD-Score:3.86|P-value:5.66E-5||SGD DESC:Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress Gene:YGP1(YNL160W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Cell wall-related secretory glycoprotein; induced by nutrient deprivation-associated growth arrest and upon entry into stationary phase; may be involved in adaptation prior to stationary phase entry; has similarity to Sps100p Gene:YHL008C(YHL008C_p)|FD-Score:4.77|P-value:9.16E-7||SGD DESC:Putative protein of unknown function, may be involved in the uptake of chloride ions; does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YHR097C(YHR097C_p)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus Gene:YIL025C(YIL025C_d)|FD-Score:-3.14|P-value:8.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL069W(YKL069W)|FD-Score:3.88|P-value:5.26E-5||SGD DESC:Methionine-R-sulfoxide reductase; reduces the R enantiomer of free Met-SO, in contrast to Ycl033Cp which reduces Met-R-SO in a peptide linkage; has a role in protection against oxidative stress; relative distribution to the nucleus increases upon DNA replication stress Gene:YNL190W(YNL190W_p)|FD-Score:6.19|P-value:3.02E-10||SGD DESC:Hydrophilin essential in dessication-rehydration process; cell wall protein; contains a putative GPI-attachment site Gene:YNL195C(YNL195C_p)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOR114W(YOR114W_p)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Putative protein of unknown function; null mutant is viable Gene:YOR366W(YOR366W_d)|FD-Score:4.19|P-value:1.42E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C Gene:YPR108W-A(YPR108W-A_p)|FD-Score:3.94|P-value:4.06E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPR127W(YPR127W)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Putative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YIL026C4.885.36E-71.13IRR1Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability
YPL251W_d4.562.56E-61.13YPL251W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C
YIL150C3.433.04E-40.00MCM10Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins
YGR191W3.423.09E-40.17HIP1High-affinity histidine permease, also involved in the transport of manganese ions
YOL038W3.255.69E-40.04PRE6Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress
YPL209C3.216.55E-40.12IPL1Aurora kinase subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), involved in regulating kinetochore-microtubule attachments; helps maintain condensed chromosomes during anaphase and early telophase; required for SPB cohesion and prevention of multipolar spindle formation; localizes to nuclear foci that become diffuse upon DNA replication stress; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon SAC arrest
YBR256C3.109.76E-40.06RIB5Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway
YGL055W3.040.001190.25OLE1Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
YHR007C2.790.002640.23ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YDR526C_d2.560.005170.01YDR526C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YPL016W2.560.005250.01SWI1Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer
YPR165W2.540.005480.04RHO1GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
YJL195C_d2.500.006170.00YJL195C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6
YER094C2.500.006190.14PUP3Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10
YGL044C2.360.009084.98E-4RNA15Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR007C_p8.239.38E-17DSF2_pDeletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress
YLR061W6.805.18E-12RPL22ARibosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication
YIR034C6.202.81E-10LYS1Saccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; also has mRNA binding activity
YNL190W_p6.193.02E-10YNL190W_pHydrophilin essential in dessication-rehydration process; cell wall protein; contains a putative GPI-attachment site
YBR279W5.639.00E-9PAF1Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1
YPL174C5.571.29E-8NIP100Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued)
YDR138W5.364.10E-8HPR1Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p
YOR322C5.191.05E-7LDB19Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye
YDR500C5.032.48E-7RPL37BRibosomal 60S subunit protein L37B; protein abundance increases in response to DNA replication stress; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37B has a paralog, RPL37A, that arose from the whole genome duplication
YHL008C_p4.779.16E-7YHL008C_pPutative protein of unknown function, may be involved in the uptake of chloride ions; does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
YBL049W4.711.23E-6MOH1Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase
YER116C4.592.18E-6SLX8Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachment of SUMO to the substrate; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress
YGL025C4.582.29E-6PGD1Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor
YKR072C4.523.15E-6SIS2Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis
YDR032C4.415.16E-6PST2Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication

GO enrichment analysis for SGTC_1021
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1151.14E-18SGTC_200481-0013 4.9 μMChemDiv (Drug-like library)1820450.142857
0.0832.05E-10SGTC_1068loratadine 26.9 μMMiscellaneous39570.1625
0.0807.90E-10SGTC_1768st044301 37.2 μMTimTec (Natural product derivative library)39313490.076087
0.0648.00E-7SGTC_29889073640 71.4 μMChembridge (Drug-like library)91022290.115942
0.0621.78E-6SGTC_2674ligustilide 100.0 μMMicrosource (Natural product library)53190220.056338
0.0622.23E-6SGTC_1071trifluoperazine 6.3 μMNIH Clinical Collection29135350.0617284
0.0612.48E-6SGTC_15447-methoxy-4-methylcoumarin 105.0 μMTimTec (Pure natural product library)3908070.0597015
0.0571.30E-5SGTC_130199-0087 65.0 μMChemDiv (Drug-like library)45898250.241935ERG2
0.0561.55E-5SGTC_7911348-1292 225.0 μMChemDiv (Drug-like library)54370540.119048
0.0561.62E-5SGTC_3793970-0972 5.9 μMChemDiv (Drug-like library)28980420.0804598tubulin folding & SWR complex
0.0552.31E-5SGTC_20955432005 200.0 μMChembridge (Fragment library)7837340.047619
0.0542.86E-5SGTC_1892st058411 7.4 μMTimTec (Natural product derivative library)39347380.142857
0.0543.81E-5SGTC_597k018-0003 82.8 μMChemDiv (Drug-like library)67862340.102273RNA pol III & RNase P/MRP
0.0533.99E-5SGTC_1688st021091 57.6 μMTimTec (Natural product derivative library)36563660.0333333
0.0534.18E-5SGTC_13271436-0082 2.2 μMChemDiv (Drug-like library)159922050.119403

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1608st00147340.5 μM0.3333337292267TimTec (Natural product derivative library)242.700244.36601
SGTC_1726st03555614.58 μM0.3333335919439TimTec (Natural product derivative library)260.6907034.57102
SGTC_2552juarezic acid87.02 μM0.3265311549512Microsource (Natural product library)174.19592.39512TSC3-RPN4
SGTC_1603st00204567.8 μM0.2542375887985TimTec (Natural product derivative library)295.1357635.23602TSC3-RPN4
SGTC_2471546695624.79 μM0.245614724419Miscellaneous274.699043.57713mitochondrial processes
SGTC_552k242-0005476 μM0.2428574088318ChemDiv (Drug-like library)419.926525.8313
SGTC_130199-008765 μM0.2419354589825ChemDiv (Drug-like library)354.229326.15902ERG2
SGTC_24605552655174.22 μM0.2419355401989Miscellaneous259.6912.62513amide catabolism
SGTC_1691st01993384.6 μM0.2413796176082TimTec (Natural product derivative library)236.308344.67401
SGTC_660109-018742.4 μM0.236364759417ChemDiv (Drug-like library)228.676963.93511