4507-0395

1-[1-(3-fluoro-4-methoxyphenyl)-2,5-dioxopyrrolidin-3-yl]piperidine-4-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1024
Screen concentration 26.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 4201836
SMILES COC1=C(C=C(C=C1)N2C(=O)CC(C2=O)N3CCC(CC3)C(=O)N)F
Standardized SMILES COc1ccc(cc1F)N2C(=O)CC(N3CCC(CC3)C(=O)N)C2=O
Molecular weight 349.3568
ALogP 0.68
H-bond donor count 1
H-bond acceptor count 6
Response signature TSC3-RPN4

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.86
% growth inhibition (Hom. pool) 5.25


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4201836
Download HIP data (tab-delimited text)  (excel)
Gene:CCT3(YJL014W)|FD-Score:4.59|P-value:2.25E-6|Clearance:0.05||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:FBA1(YKL060C)|FD-Score:-3.2|P-value:6.89E-4|Clearance:0||SGD DESC:Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress Gene:GCD2(YGR083C)|FD-Score:3.6|P-value:1.62E-4|Clearance:0.09||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GPI19(YDR437W)|FD-Score:4.79|P-value:8.53E-7|Clearance:0.15||SGD DESC:Subunit of GPI-GlcNAc transferase involved in synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), which is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis, shares similarity with mammalian PIG-P Gene:KRR1(YCL059C)|FD-Score:4.64|P-value:1.78E-6|Clearance:0.05||SGD DESC:Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit Gene:NIP1(YMR309C)|FD-Score:3.17|P-value:7.65E-4|Clearance:0.01||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:NOP14(YDL148C)|FD-Score:3.73|P-value:9.48E-5|Clearance:0.14||SGD DESC:Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:PGS1(YCL004W)|FD-Score:4.54|P-value:2.81E-6|Clearance:0.03||SGD DESC:Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis Gene:PRE8(YML092C)|FD-Score:3.83|P-value:6.36E-5|Clearance:0.1||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:PRP42(YDR235W)|FD-Score:3.43|P-value:2.97E-4|Clearance:0.21||SGD DESC:U1 snRNP protein involved in splicing, required for U1 snRNP biogenesis; contains multiple tetriatricopeptide repeats Gene:RBA50(YDR527W)|FD-Score:4.22|P-value:1.23E-5|Clearance:0.38||SGD DESC:Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 Gene:RET1(YOR207C)|FD-Score:4.92|P-value:4.22E-7|Clearance:0.14||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RPB10(YOR210W)|FD-Score:4.5|P-value:3.35E-6|Clearance:0.28||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPB11(YOL005C)|FD-Score:5.45|P-value:2.54E-8|Clearance:0.52||SGD DESC:RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit Gene:RPN5(YDL147W)|FD-Score:3.15|P-value:8.28E-4|Clearance:0.18||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RPP1(YHR062C)|FD-Score:9.14|P-value:3.16E-20|Clearance:3.69||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS31(YLR167W)|FD-Score:3.51|P-value:2.24E-4|Clearance:0.08||SGD DESC:Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog Gene:RRS1(YOR294W)|FD-Score:-4.29|P-value:8.89E-6|Clearance:0||SGD DESC:Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice Gene:RTP1(YMR185W_p)|FD-Score:3.84|P-value:6.25E-5|Clearance:0||SGD DESC:Protein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene Gene:SDA1(YGR245C)|FD-Score:3.22|P-value:6.30E-4|Clearance:0.06||SGD DESC:Highly conserved nuclear protein required for actin cytoskeleton organization and passage through Start, plays a critical role in G1 events, binds Nap1p, also involved in 60S ribosome biogenesis Gene:SPC98(YNL126W)|FD-Score:-3.14|P-value:8.54E-4|Clearance:0||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:TAF8(YML114C)|FD-Score:3.16|P-value:7.79E-4|Clearance:0.02||SGD DESC:TFIID subunit (65 kDa), involved in RNA polymerase II transcription initiation Gene:TFC3(YAL001C)|FD-Score:-4.9|P-value:4.76E-7|Clearance:0||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:TOP2(YNL088W)|FD-Score:4.51|P-value:3.23E-6|Clearance:0.01||SGD DESC:Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:TSC11(YER093C)|FD-Score:-3.45|P-value:2.82E-4|Clearance:0||SGD DESC:Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p), a membrane-associated complex that regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; involved in sphingolipid metabolism; contains a RasGEFN domain Gene:CCT3(YJL014W)|FD-Score:4.59|P-value:2.25E-6|Clearance:0.05||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:FBA1(YKL060C)|FD-Score:-3.2|P-value:6.89E-4|Clearance:0||SGD DESC:Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress Gene:GCD2(YGR083C)|FD-Score:3.6|P-value:1.62E-4|Clearance:0.09||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GPI19(YDR437W)|FD-Score:4.79|P-value:8.53E-7|Clearance:0.15||SGD DESC:Subunit of GPI-GlcNAc transferase involved in synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), which is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis, shares similarity with mammalian PIG-P Gene:KRR1(YCL059C)|FD-Score:4.64|P-value:1.78E-6|Clearance:0.05||SGD DESC:Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit Gene:NIP1(YMR309C)|FD-Score:3.17|P-value:7.65E-4|Clearance:0.01||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:NOP14(YDL148C)|FD-Score:3.73|P-value:9.48E-5|Clearance:0.14||SGD DESC:Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:PGS1(YCL004W)|FD-Score:4.54|P-value:2.81E-6|Clearance:0.03||SGD DESC:Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis Gene:PRE8(YML092C)|FD-Score:3.83|P-value:6.36E-5|Clearance:0.1||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:PRP42(YDR235W)|FD-Score:3.43|P-value:2.97E-4|Clearance:0.21||SGD DESC:U1 snRNP protein involved in splicing, required for U1 snRNP biogenesis; contains multiple tetriatricopeptide repeats Gene:RBA50(YDR527W)|FD-Score:4.22|P-value:1.23E-5|Clearance:0.38||SGD DESC:Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 Gene:RET1(YOR207C)|FD-Score:4.92|P-value:4.22E-7|Clearance:0.14||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RPB10(YOR210W)|FD-Score:4.5|P-value:3.35E-6|Clearance:0.28||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPB11(YOL005C)|FD-Score:5.45|P-value:2.54E-8|Clearance:0.52||SGD DESC:RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit Gene:RPN5(YDL147W)|FD-Score:3.15|P-value:8.28E-4|Clearance:0.18||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RPP1(YHR062C)|FD-Score:9.14|P-value:3.16E-20|Clearance:3.69||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS31(YLR167W)|FD-Score:3.51|P-value:2.24E-4|Clearance:0.08||SGD DESC:Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog Gene:RRS1(YOR294W)|FD-Score:-4.29|P-value:8.89E-6|Clearance:0||SGD DESC:Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice Gene:RTP1(YMR185W_p)|FD-Score:3.84|P-value:6.25E-5|Clearance:0||SGD DESC:Protein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene Gene:SDA1(YGR245C)|FD-Score:3.22|P-value:6.30E-4|Clearance:0.06||SGD DESC:Highly conserved nuclear protein required for actin cytoskeleton organization and passage through Start, plays a critical role in G1 events, binds Nap1p, also involved in 60S ribosome biogenesis Gene:SPC98(YNL126W)|FD-Score:-3.14|P-value:8.54E-4|Clearance:0||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:TAF8(YML114C)|FD-Score:3.16|P-value:7.79E-4|Clearance:0.02||SGD DESC:TFIID subunit (65 kDa), involved in RNA polymerase II transcription initiation Gene:TFC3(YAL001C)|FD-Score:-4.9|P-value:4.76E-7|Clearance:0||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:TOP2(YNL088W)|FD-Score:4.51|P-value:3.23E-6|Clearance:0.01||SGD DESC:Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:TSC11(YER093C)|FD-Score:-3.45|P-value:2.82E-4|Clearance:0||SGD DESC:Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p), a membrane-associated complex that regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; involved in sphingolipid metabolism; contains a RasGEFN domain

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4201836
Download HOP data (tab-delimited text)  (excel)
Gene:ACE2(YLR131C)|FD-Score:5.97|P-value:1.22E-9||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:ADE1(YAR015W)|FD-Score:-5.47|P-value:2.23E-8||SGD DESC:N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress Gene:ADY4(YLR227C)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:Structural component of the meiotic outer plaque, which is a membrane-organizing center that assembles on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane Gene:ALD3(YMR169C)|FD-Score:4.1|P-value:2.10E-5||SGD DESC:Cytoplasmic aldehyde dehydrogenase, involved in beta-alanine synthesis; uses NAD+ as the preferred coenzyme; very similar to Ald2p; expression is induced by stress and repressed by glucose Gene:ALD6(YPL061W)|FD-Score:5.03|P-value:2.42E-7||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:ALF1(YNL148C)|FD-Score:5.99|P-value:1.03E-9||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ARP1(YHR129C)|FD-Score:3.92|P-value:4.48E-5||SGD DESC:Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin Gene:BUD17(YNR027W)|FD-Score:4.41|P-value:5.17E-6||SGD DESC:Putative pyridoxal kinase, a key enzyme in vitamin B6 metabolism; involved in bud-site selection; diploid mutants display a random rather than a bipolar budding pattern; similarity to yeast BUD16 and human pyridoxal kinase (PDXK) Gene:CNM67(YNL225C)|FD-Score:3.1|P-value:9.66E-4||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:COG1(YGL223C)|FD-Score:-3.21|P-value:6.54E-4||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COS12(YGL263W)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:DAS1(YJL149W)|FD-Score:4.03|P-value:2.81E-5||SGD DESC:Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C Gene:DSE4(YNR067C)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother Gene:GLC8(YMR311C)|FD-Score:4.79|P-value:8.41E-7||SGD DESC:Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress Gene:GLY1(YEL046C)|FD-Score:3.14|P-value:8.37E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GMC1(YDR506C)|FD-Score:3.97|P-value:3.53E-5||SGD DESC:Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; possible membrane-localized protein Gene:HPC2(YBR215W)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; mutants display synthetic defects with subunits of FACT, a complex that allows passage of RNA Pol II through nucleosomes Gene:HXK1(YFR053C)|FD-Score:4.04|P-value:2.62E-5||SGD DESC:Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication Gene:ILV1(YER086W)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation Gene:IRC10(YOL015W)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:IZH2(YOL002C)|FD-Score:4.77|P-value:9.25E-7||SGD DESC:Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc Gene:MAK3(YPR051W)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus Gene:MMM1(YLL006W)|FD-Score:-4.24|P-value:1.14E-5||SGD DESC:ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MRPL16(YBL038W)|FD-Score:3.93|P-value:4.16E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSH2(YOL090W)|FD-Score:5.11|P-value:1.65E-7||SGD DESC:Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP Gene:NAS6(YGR232W)|FD-Score:3.24|P-value:6.01E-4||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); ortholog of human oncoprotein gankyrin, which interacts with the Rb tumor suppressor and CDK4/6 Gene:NIP100(YPL174C)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) Gene:NPP1(YCR026C)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP1 has a paralog, NPP2, that arose from the whole genome duplication Gene:NRG1(YDR043C)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Transcriptional repressor; recruits the Cyc8p-Tup1p complex to promoters; mediates glucose repression and negatively regulates a variety of processes including filamentous growth and alkaline pH response; NRG1 has a paralog, NRG2, that arose from the whole genome duplication Gene:PCP1(YGR101W)|FD-Score:4.71|P-value:1.23E-6||SGD DESC:Mitochondrial serine protease required for the processing of various mitochondrial proteins and maintenance of mitochondrial DNA and morphology; belongs to the rhomboid-GlpG superfamily of intramembrane peptidases Gene:PDR12(YPL058C)|FD-Score:3.19|P-value:7.14E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, weak-acid-inducible multidrug transporter required for weak organic acid resistance; induced by sorbate and benzoate and regulated by War1p; mutants exhibit sorbate hypersensitivity Gene:PTH2(YBL057C)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth Gene:RAD16(YBR114W)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:RAD27(YKL113C)|FD-Score:-3.12|P-value:9.10E-4||SGD DESC:5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family Gene:RAI1(YGL246C)|FD-Score:4.31|P-value:8.34E-6||SGD DESC:Nuclear protein with decapping endonuclease activity targeted toward mRNAs with unmethylated 7-methylguanosine cap structures; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; homologous to human DOM3Z Gene:RBG1(YAL036C)|FD-Score:-3.31|P-value:4.68E-4||SGD DESC:Member of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid Gene:RML2(YEL050C)|FD-Score:-3.23|P-value:6.25E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:RPL20A(YMR242C)|FD-Score:-3.14|P-value:8.44E-4||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RPL43A(YPR043W)|FD-Score:5.64|P-value:8.57E-9||SGD DESC:Ribosomal 60S subunit protein L43A; null mutation confers a dominant lethal phenotype; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43A has a paralog, RPL43B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:5.74|P-value:4.76E-9||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS0B(YLR048W)|FD-Score:3.37|P-value:3.82E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2 Gene:RRG7(YOR305W)|FD-Score:4.9|P-value:4.73E-7||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YOR305W is not an essential gene Gene:SEY1(YOR165W)|FD-Score:4.35|P-value:6.77E-6||SGD DESC:Dynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3 Gene:SLF1(YDR515W)|FD-Score:3.72|P-value:9.90E-5||SGD DESC:RNA binding protein that associates with polysomes; may be involved in regulating mRNA translation; involved in the copper-dependent mineralization of copper sulfide complexes on cell surface in cells cultured in copper salts; SLF1 has a paralog, SRO9, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SNF12(YNR023W)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive Gene:SNF6(YHL025W)|FD-Score:-4.2|P-value:1.34E-5||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions Gene:STL1(YDR536W)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Glycerol proton symporter of the plasma membrane, subject to glucose-induced inactivation, strongly but transiently induced when cells are subjected to osmotic shock Gene:STM1(YLR150W)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery Gene:THP3(YPR045C)|FD-Score:5.42|P-value:3.02E-8||SGD DESC:Protein that forms a complex with Csn12p that is recruited to transcribed genes and may have a role in transcription elongation; possibly involved in splicing based on pre-mRNA accumulation defect for many intron-containing genes Gene:TMA19(YKL056C)|FD-Score:3.83|P-value:6.49E-5||SGD DESC:Protein that associates with ribosomes; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and relocates to the mitochondrial outer surface upon oxidative stress Gene:TRP2(YER090W)|FD-Score:4.23|P-value:1.19E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP4(YDR354W)|FD-Score:5.04|P-value:2.27E-7||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:15.7|P-value:3.89E-56||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUB3(YML124C)|FD-Score:9.01|P-value:1.01E-19||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UBP9(YER098W)|FD-Score:-3.17|P-value:7.61E-4||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP9 has a paralog, UBP13, that arose from the whole genome duplication Gene:URK1(YNR012W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Uridine/cytidine kinase, component of the pyrimidine ribonucleotide salvage pathway that converts uridine into UMP and cytidine into CMP; involved in the pyrimidine deoxyribonucleotide salvage pathway, converting deoxycytidine into dCMP Gene:VAC17(YCL063W)|FD-Score:3.86|P-value:5.59E-5||SGD DESC:Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p Gene:VPS75(YNL246W)|FD-Score:3.89|P-value:5.05E-5||SGD DESC:NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress Gene:YAL044W-A(YAL044W-A_p)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Putative protein of unknown function; similar to S. pombe uvi31 which is a putative DNA repair protein Gene:YAP1(YML007W)|FD-Score:3.31|P-value:4.63E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YBR226C(YBR226C_d)|FD-Score:-3.37|P-value:3.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W Gene:YCL001W-A(YCL001W-A_p)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III Gene:YCR095W-A(YCR095W-A_p)|FD-Score:3.31|P-value:4.64E-4||SGD DESC:Putative protein of unknown function Gene:YDR182W-A(YDR182W-A_p)|FD-Score:5.57|P-value:1.28E-8||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YHP1(YDR451C)|FD-Score:-3.71|P-value:1.04E-4||SGD DESC:Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication Gene:YKL091C(YKL091C)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Putative homolog of Sec14p, which is a phosphatidylinositol/phosphatidylcholine transfer protein involved in lipid metabolism; localizes to the nucleus; contains a CRAL/TRIO domain and binds several lipids in a large-scale study Gene:YLR122C(YLR122C_d)|FD-Score:3.93|P-value:4.33E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR123C Gene:YML020W(YML020W_p)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Putative protein of unknown function Gene:YSC83(YHR017W)|FD-Score:4.76|P-value:9.76E-7||SGD DESC:Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83 Gene:ACE2(YLR131C)|FD-Score:5.97|P-value:1.22E-9||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:ADE1(YAR015W)|FD-Score:-5.47|P-value:2.23E-8||SGD DESC:N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress Gene:ADY4(YLR227C)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:Structural component of the meiotic outer plaque, which is a membrane-organizing center that assembles on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane Gene:ALD3(YMR169C)|FD-Score:4.1|P-value:2.10E-5||SGD DESC:Cytoplasmic aldehyde dehydrogenase, involved in beta-alanine synthesis; uses NAD+ as the preferred coenzyme; very similar to Ald2p; expression is induced by stress and repressed by glucose Gene:ALD6(YPL061W)|FD-Score:5.03|P-value:2.42E-7||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:ALF1(YNL148C)|FD-Score:5.99|P-value:1.03E-9||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ARP1(YHR129C)|FD-Score:3.92|P-value:4.48E-5||SGD DESC:Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin Gene:BUD17(YNR027W)|FD-Score:4.41|P-value:5.17E-6||SGD DESC:Putative pyridoxal kinase, a key enzyme in vitamin B6 metabolism; involved in bud-site selection; diploid mutants display a random rather than a bipolar budding pattern; similarity to yeast BUD16 and human pyridoxal kinase (PDXK) Gene:CNM67(YNL225C)|FD-Score:3.1|P-value:9.66E-4||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:COG1(YGL223C)|FD-Score:-3.21|P-value:6.54E-4||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COS12(YGL263W)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:DAS1(YJL149W)|FD-Score:4.03|P-value:2.81E-5||SGD DESC:Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C Gene:DSE4(YNR067C)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother Gene:GLC8(YMR311C)|FD-Score:4.79|P-value:8.41E-7||SGD DESC:Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress Gene:GLY1(YEL046C)|FD-Score:3.14|P-value:8.37E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GMC1(YDR506C)|FD-Score:3.97|P-value:3.53E-5||SGD DESC:Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; possible membrane-localized protein Gene:HPC2(YBR215W)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; mutants display synthetic defects with subunits of FACT, a complex that allows passage of RNA Pol II through nucleosomes Gene:HXK1(YFR053C)|FD-Score:4.04|P-value:2.62E-5||SGD DESC:Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication Gene:ILV1(YER086W)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation Gene:IRC10(YOL015W)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:IZH2(YOL002C)|FD-Score:4.77|P-value:9.25E-7||SGD DESC:Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc Gene:MAK3(YPR051W)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus Gene:MMM1(YLL006W)|FD-Score:-4.24|P-value:1.14E-5||SGD DESC:ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MRPL16(YBL038W)|FD-Score:3.93|P-value:4.16E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSH2(YOL090W)|FD-Score:5.11|P-value:1.65E-7||SGD DESC:Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP Gene:NAS6(YGR232W)|FD-Score:3.24|P-value:6.01E-4||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); ortholog of human oncoprotein gankyrin, which interacts with the Rb tumor suppressor and CDK4/6 Gene:NIP100(YPL174C)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) Gene:NPP1(YCR026C)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP1 has a paralog, NPP2, that arose from the whole genome duplication Gene:NRG1(YDR043C)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Transcriptional repressor; recruits the Cyc8p-Tup1p complex to promoters; mediates glucose repression and negatively regulates a variety of processes including filamentous growth and alkaline pH response; NRG1 has a paralog, NRG2, that arose from the whole genome duplication Gene:PCP1(YGR101W)|FD-Score:4.71|P-value:1.23E-6||SGD DESC:Mitochondrial serine protease required for the processing of various mitochondrial proteins and maintenance of mitochondrial DNA and morphology; belongs to the rhomboid-GlpG superfamily of intramembrane peptidases Gene:PDR12(YPL058C)|FD-Score:3.19|P-value:7.14E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, weak-acid-inducible multidrug transporter required for weak organic acid resistance; induced by sorbate and benzoate and regulated by War1p; mutants exhibit sorbate hypersensitivity Gene:PTH2(YBL057C)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth Gene:RAD16(YBR114W)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:RAD27(YKL113C)|FD-Score:-3.12|P-value:9.10E-4||SGD DESC:5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family Gene:RAI1(YGL246C)|FD-Score:4.31|P-value:8.34E-6||SGD DESC:Nuclear protein with decapping endonuclease activity targeted toward mRNAs with unmethylated 7-methylguanosine cap structures; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; homologous to human DOM3Z Gene:RBG1(YAL036C)|FD-Score:-3.31|P-value:4.68E-4||SGD DESC:Member of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid Gene:RML2(YEL050C)|FD-Score:-3.23|P-value:6.25E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:RPL20A(YMR242C)|FD-Score:-3.14|P-value:8.44E-4||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RPL43A(YPR043W)|FD-Score:5.64|P-value:8.57E-9||SGD DESC:Ribosomal 60S subunit protein L43A; null mutation confers a dominant lethal phenotype; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43A has a paralog, RPL43B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:5.74|P-value:4.76E-9||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS0B(YLR048W)|FD-Score:3.37|P-value:3.82E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2 Gene:RRG7(YOR305W)|FD-Score:4.9|P-value:4.73E-7||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YOR305W is not an essential gene Gene:SEY1(YOR165W)|FD-Score:4.35|P-value:6.77E-6||SGD DESC:Dynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3 Gene:SLF1(YDR515W)|FD-Score:3.72|P-value:9.90E-5||SGD DESC:RNA binding protein that associates with polysomes; may be involved in regulating mRNA translation; involved in the copper-dependent mineralization of copper sulfide complexes on cell surface in cells cultured in copper salts; SLF1 has a paralog, SRO9, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SNF12(YNR023W)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive Gene:SNF6(YHL025W)|FD-Score:-4.2|P-value:1.34E-5||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions Gene:STL1(YDR536W)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Glycerol proton symporter of the plasma membrane, subject to glucose-induced inactivation, strongly but transiently induced when cells are subjected to osmotic shock Gene:STM1(YLR150W)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery Gene:THP3(YPR045C)|FD-Score:5.42|P-value:3.02E-8||SGD DESC:Protein that forms a complex with Csn12p that is recruited to transcribed genes and may have a role in transcription elongation; possibly involved in splicing based on pre-mRNA accumulation defect for many intron-containing genes Gene:TMA19(YKL056C)|FD-Score:3.83|P-value:6.49E-5||SGD DESC:Protein that associates with ribosomes; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and relocates to the mitochondrial outer surface upon oxidative stress Gene:TRP2(YER090W)|FD-Score:4.23|P-value:1.19E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP4(YDR354W)|FD-Score:5.04|P-value:2.27E-7||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:15.7|P-value:3.89E-56||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUB3(YML124C)|FD-Score:9.01|P-value:1.01E-19||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UBP9(YER098W)|FD-Score:-3.17|P-value:7.61E-4||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP9 has a paralog, UBP13, that arose from the whole genome duplication Gene:URK1(YNR012W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Uridine/cytidine kinase, component of the pyrimidine ribonucleotide salvage pathway that converts uridine into UMP and cytidine into CMP; involved in the pyrimidine deoxyribonucleotide salvage pathway, converting deoxycytidine into dCMP Gene:VAC17(YCL063W)|FD-Score:3.86|P-value:5.59E-5||SGD DESC:Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p Gene:VPS75(YNL246W)|FD-Score:3.89|P-value:5.05E-5||SGD DESC:NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress Gene:YAL044W-A(YAL044W-A_p)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Putative protein of unknown function; similar to S. pombe uvi31 which is a putative DNA repair protein Gene:YAP1(YML007W)|FD-Score:3.31|P-value:4.63E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YBR226C(YBR226C_d)|FD-Score:-3.37|P-value:3.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W Gene:YCL001W-A(YCL001W-A_p)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III Gene:YCR095W-A(YCR095W-A_p)|FD-Score:3.31|P-value:4.64E-4||SGD DESC:Putative protein of unknown function Gene:YDR182W-A(YDR182W-A_p)|FD-Score:5.57|P-value:1.28E-8||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YHP1(YDR451C)|FD-Score:-3.71|P-value:1.04E-4||SGD DESC:Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication Gene:YKL091C(YKL091C)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Putative homolog of Sec14p, which is a phosphatidylinositol/phosphatidylcholine transfer protein involved in lipid metabolism; localizes to the nucleus; contains a CRAL/TRIO domain and binds several lipids in a large-scale study Gene:YLR122C(YLR122C_d)|FD-Score:3.93|P-value:4.33E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR123C Gene:YML020W(YML020W_p)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Putative protein of unknown function Gene:YSC83(YHR017W)|FD-Score:4.76|P-value:9.76E-7||SGD DESC:Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR062C9.143.16E-203.69RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YOL005C5.452.54E-80.52RPB11RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit
YOR207C4.924.22E-70.14RET1Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
YDR437W4.798.53E-70.15GPI19Subunit of GPI-GlcNAc transferase involved in synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), which is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis, shares similarity with mammalian PIG-P
YCL059C4.641.78E-60.05KRR1Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit
YJL014W4.592.25E-60.05CCT3Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YCL004W4.542.81E-60.03PGS1Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis
YNL088W4.513.23E-60.01TOP2Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation
YOR210W4.503.35E-60.28RPB10RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III
YDR527W4.221.23E-50.38RBA50Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1
YMR185W_p3.846.25E-50.00RTP1_pProtein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene
YML092C3.836.36E-50.10PRE8Alpha 2 subunit of the 20S proteasome
YDL148C3.739.48E-50.14NOP14Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA
YGR083C3.601.62E-40.09GCD2Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YLR167W3.512.24E-40.08RPS31Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A15.703.89E-56TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YML124C9.011.01E-19TUB3Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication
YNL148C5.991.03E-9ALF1Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance
YLR131C5.971.22E-9ACE2Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication
YDL020C5.744.76E-9RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YPR043W5.648.57E-9RPL43ARibosomal 60S subunit protein L43A; null mutation confers a dominant lethal phenotype; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43A has a paralog, RPL43B, that arose from the whole genome duplication
YDR182W-A_p5.571.28E-8YDR182W-A_pPutative protein of unknown function; identified by fungal homology and RT-PCR
YPR045C5.423.02E-8THP3Protein that forms a complex with Csn12p that is recruited to transcribed genes and may have a role in transcription elongation; possibly involved in splicing based on pre-mRNA accumulation defect for many intron-containing genes
YOL090W5.111.65E-7MSH2Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP
YDR354W5.042.27E-7TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YPL061W5.032.42E-7ALD6Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress
YOR305W4.904.73E-7RRG7Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YOR305W is not an essential gene
YMR311C4.798.41E-7GLC8Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress
YOL002C4.779.25E-7IZH2Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc
YHR017W4.769.76E-7YSC83Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83

GO enrichment analysis for SGTC_1024
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.3891.08E-212SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.202532
0.3798.92E-201SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.108108TSC3-RPN4
0.3473.70E-166SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0810811TSC3-RPN4
0.3428.65E-162SGTC_32829108498 15.5 μMChembridge (Drug-like library)164596450.185185
0.3395.62E-159SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.168675TSC3-RPN4
0.3294.06E-149SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.123288TSC3-RPN4
0.3146.59E-135SGTC_23236148992 148.3 μMChembridge (Fragment library)28882200.118421
0.3103.75E-131SGTC_8971000-0139 31.4 μMChemDiv (Drug-like library)61510200.208955
0.3053.99E-127SGTC_9262268-1401 59.4 μMChemDiv (Drug-like library)29315780.0697674TSC3-RPN4
0.3003.52E-123SGTC_20905301565 104.4 μMChembridge (Fragment library)20563440.08TSC3-RPN4
0.2954.68E-119SGTC_1662st014054 52.0 μMTimTec (Natural product derivative library)28387770.0989011TSC3-RPN4
0.2911.89E-115SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.114286TSC3-RPN4
0.2851.23E-110SGTC_11730443-0020 122.0 μMChemDiv (Drug-like library)53312950.136986TSC3-RPN4
0.2852.32E-110SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.0897436TSC3-RPN4
0.2852.38E-110SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.0843373TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_3294912295326.28 μM0.36111116459826Chembridge (Drug-like library)394.420482.38806tubulin folding & SWR complex
SGTC_7103448-418758 μM0.3285713946412ChemDiv (Drug-like library)509.767813.79904fatty acid desaturase (OLE1)
SGTC_2465580924233.21 μM0.301372731748Miscellaneous357.38042.68116
SGTC_2123807-468238.77 μM0.2739733125501ChemDiv (Drug-like library)304.324381.35316tubulin folding & SWR complex
SGTC_5433844-1095191 μM0.2602744230703ChemDiv (Drug-like library)354.399682.70205ERG2
SGTC_2469565501990.81 μM0.2602742788239Miscellaneous341.3813.18315
SGTC_2487591837344.05 μM0.259742876279Miscellaneous385.39052.46517
SGTC_2103551-044556.83 μM0.256412865180ChemDiv (Drug-like library)371.406983.0316
SGTC_2483572905027.16 μM0.256412865180Miscellaneous371.406983.0316
SGTC_2476580691610.51 μM0.256412870194Miscellaneous395.352393.63918