k015-0029

1-[(3,4-dichlorophenyl)methyl]-5-nitroindole-2,3-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1028
Screen concentration 4.8 μM
Source ChemDiv (Drug-like library)
PubChem CID 4588309
SMILES C1=CC(=C(C=C1CN2C3=C(C=C(C=C3)[N+](=O)[O-])C(=O)C2=O)Cl)Cl
Standardized SMILES [O-][N+](=O)c1ccc2N(Cc3ccc(Cl)c(Cl)c3)C(=O)C(=O)c2c1
Molecular weight 351.141
ALogP 3.68
H-bond donor count 0
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 15.4
% growth inhibition (Hom. pool) 4.85


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4588309
Download HIP data (tab-delimited text)  (excel)
Gene:CBK1(YNL161W)|FD-Score:-4.05|P-value:2.57E-5|Clearance:0||SGD DESC:Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress Gene:CCL1(YPR025C)|FD-Score:4.57|P-value:2.48E-6|Clearance:0.15||SGD DESC:Cyclin associated with protein kinase Kin28p, which is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters Gene:DYS1(YHR068W)|FD-Score:3.21|P-value:6.63E-4|Clearance:0.05||SGD DESC:Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric Gene:GCD1(YOR260W)|FD-Score:-3.18|P-value:7.31E-4|Clearance:0||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GPI18(YBR004C)|FD-Score:3.16|P-value:7.93E-4|Clearance:0.11||SGD DESC:Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria Gene:ISD11(YER048W-A)|FD-Score:-3.25|P-value:5.86E-4|Clearance:0||SGD DESC:Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis Gene:MED6(YHR058C)|FD-Score:-3.76|P-value:8.60E-5|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress Gene:PAB1(YER165W)|FD-Score:5.39|P-value:3.44E-8|Clearance:0.83||SGD DESC:Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G Gene:POP1(YNL221C)|FD-Score:3.59|P-value:1.68E-4|Clearance:0.33||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:RER2(YBR002C)|FD-Score:3.26|P-value:5.60E-4|Clearance:0.05||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:ROK1(YGL171W)|FD-Score:-3.39|P-value:3.50E-4|Clearance:0||SGD DESC:RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation Gene:RPL18A(YOL120C)|FD-Score:3.62|P-value:1.49E-4|Clearance:0.03||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RVB2(YPL235W)|FD-Score:3.74|P-value:9.17E-5|Clearance:0.12||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SEC1(YDR164C)|FD-Score:-3.48|P-value:2.46E-4|Clearance:0||SGD DESC:Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function Gene:SFH1(YLR321C)|FD-Score:4.42|P-value:5.00E-6|Clearance:0.68||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:CBK1(YNL161W)|FD-Score:-4.05|P-value:2.57E-5|Clearance:0||SGD DESC:Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress Gene:CCL1(YPR025C)|FD-Score:4.57|P-value:2.48E-6|Clearance:0.15||SGD DESC:Cyclin associated with protein kinase Kin28p, which is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters Gene:DYS1(YHR068W)|FD-Score:3.21|P-value:6.63E-4|Clearance:0.05||SGD DESC:Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric Gene:GCD1(YOR260W)|FD-Score:-3.18|P-value:7.31E-4|Clearance:0||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GPI18(YBR004C)|FD-Score:3.16|P-value:7.93E-4|Clearance:0.11||SGD DESC:Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria Gene:ISD11(YER048W-A)|FD-Score:-3.25|P-value:5.86E-4|Clearance:0||SGD DESC:Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis Gene:MED6(YHR058C)|FD-Score:-3.76|P-value:8.60E-5|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress Gene:PAB1(YER165W)|FD-Score:5.39|P-value:3.44E-8|Clearance:0.83||SGD DESC:Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G Gene:POP1(YNL221C)|FD-Score:3.59|P-value:1.68E-4|Clearance:0.33||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:RER2(YBR002C)|FD-Score:3.26|P-value:5.60E-4|Clearance:0.05||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:ROK1(YGL171W)|FD-Score:-3.39|P-value:3.50E-4|Clearance:0||SGD DESC:RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation Gene:RPL18A(YOL120C)|FD-Score:3.62|P-value:1.49E-4|Clearance:0.03||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RVB2(YPL235W)|FD-Score:3.74|P-value:9.17E-5|Clearance:0.12||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SEC1(YDR164C)|FD-Score:-3.48|P-value:2.46E-4|Clearance:0||SGD DESC:Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function Gene:SFH1(YLR321C)|FD-Score:4.42|P-value:5.00E-6|Clearance:0.68||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4588309
Download HOP data (tab-delimited text)  (excel)
Gene:APL6(YGR261C)|FD-Score:4.1|P-value:2.10E-5||SGD DESC:Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools Gene:ASC1(YMR116C)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; regulates P-body formation induced by replication stress; represses Gcn4p in the absence of amino acid starvation Gene:AZF1(YOR113W)|FD-Score:4.62|P-value:1.88E-6||SGD DESC:Zinc-finger transcription factor; in the presence of glucose, activates transcription of genes involved in growth and carbon metabolism; in nonfermentable carbon sources, activates transcription of genes involved in maintenance of cell wall integrity Gene:BOI2(YER114C)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; BOI2 has a paralog, BOI1, that arose from the whole genome duplication Gene:BOR1(YNL275W)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Boron efflux transporter of the plasma membrane; binds HCO3-, I-, Br-, NO3- and Cl-; has similarity to the characterized boron efflux transporter A. thaliana BOR1 Gene:BRO1(YPL084W)|FD-Score:3.73|P-value:9.64E-5||SGD DESC:Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes Gene:CMR3(YPR013C_p)|FD-Score:-3.49|P-value:2.46E-4||SGD DESC:Putative zinc finger protein; YPR013C is not an essential gene Gene:COS10(YNR075W)|FD-Score:-3.27|P-value:5.30E-4||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:CRH1(YGR189C)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar and functionally redundant to Utr2; localizes to sites of polarized growth; expression induced by cell wall stress Gene:DAP1(YPL170W)|FD-Score:-3.12|P-value:9.00E-4||SGD DESC:Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis Gene:ECM30(YLR436C)|FD-Score:4.21|P-value:1.26E-5||SGD DESC:Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants have abormal relative levels of mannose and glucose and have Gap1p sorting and transport defects; (GFP)-fusion protein localizes to the cytoplasm Gene:ELP3(YPL086C)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; exhibits histone acetyltransferase activity that is directed to histones H3 and H4; disruption confers resistance to K. lactis zymotoxin Gene:EPS1(YIL005W)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:ER protein with chaperone and co-chaperone activity, involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family Gene:ERG2(YMR202W)|FD-Score:4.71|P-value:1.26E-6||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ESC1(YMR219W)|FD-Score:3.17|P-value:7.62E-4||SGD DESC:Protein localized to the nuclear periphery, involved in telomeric silencing; interacts with PAD4-domain of Sir4p Gene:FES1(YBR101C)|FD-Score:-3.34|P-value:4.17E-4||SGD DESC:Hsp70 (Ssa1p) nucleotide exchange factor; cytosolic homolog of Sil1p, which is the nucleotide exchange factor for BiP (Kar2p) in the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:FIN1(YDR130C)|FD-Score:-4.9|P-value:4.88E-7||SGD DESC:Spindle pole body-related intermediate filament protein; forms cell cycle-specific filaments between spindle pole bodies in dividing cells; localizes to poles and microtubules of spindle during anaphase and contributes to spindle stability; involved in Glc7p localization and regulation; relative distribution to the nucleus increases upon DNA replication stress Gene:FMP48(YGR052W_p)|FD-Score:4.93|P-value:4.04E-7||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FUB1(YCR076C)|FD-Score:-3.96|P-value:3.78E-5||SGD DESC:Putative protein of unknown function; interacts physically with multiple subunits of the 20S proteasome and genetically with genes encoding 20S core particle and 19S regulatory particle subunits; exhibits boundary activity which blocks the propagation of heterochromatic silencing; contains a PI31 proteasome regulator domain and sequence similarity with human PSMF1, a proteasome inhibitor; not an essential gene Gene:FUN14(YAL008W)|FD-Score:3.15|P-value:8.30E-4||SGD DESC:Mitochondrial protein of unknown function Gene:GPT2(YKR067W)|FD-Score:-3.42|P-value:3.08E-4||SGD DESC:Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen Gene:IRC21(YMR073C)|FD-Score:5.35|P-value:4.42E-8||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:IST2(YBR086C)|FD-Score:3.12|P-value:9.03E-4||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localizes to the mother cell in small-budded cells and to the bud in medium- and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process Gene:LDB19(YOR322C)|FD-Score:4.94|P-value:3.87E-7||SGD DESC:Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye Gene:LOA1(YPR139C)|FD-Score:3.15|P-value:8.04E-4||SGD DESC:Lysophosphatidic acid acyltransferase; involved in triacelglyceride homeostasis and lipid droplet formation; localized to lipid droplets and the ER; specificity for oleoyl-CoA Gene:LYS14(YDR034C)|FD-Score:4.79|P-value:8.47E-7||SGD DESC:Transcriptional activator involved in regulation of genes of the lysine biosynthesis pathway; requires 2-aminoadipate semialdehyde as co-inducer Gene:MAE1(YKL029C)|FD-Score:4.07|P-value:2.33E-5||SGD DESC:Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids Gene:MDM20(YOL076W)|FD-Score:4.88|P-value:5.43E-7||SGD DESC:Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly Gene:MDM36(YPR083W)|FD-Score:4.13|P-value:1.84E-5||SGD DESC:Mitochondrial protein required for normal mitochondrial morphology and inheritance; proposed involvement in the formation of Dnm1p and Num1p-containing cortical anchor complexes that promote mitochondrial fission Gene:MET1(YKR069W)|FD-Score:3.27|P-value:5.37E-4||SGD DESC:S-adenosyl-L-methionine uroporphyrinogen III transmethylase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis Gene:MND2(YIR025W)|FD-Score:4.09|P-value:2.11E-5||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:MRPS35(YGR165W)|FD-Score:-3.25|P-value:5.78E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:NDL1(YLR254C)|FD-Score:4.12|P-value:1.91E-5||SGD DESC:Homolog of nuclear distribution factor NudE, NUDEL; interacts with Pac1p and regulates dynein targeting to microtubule plus ends Gene:NHP10(YDL002C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Protein related to mammalian high mobility group proteins; preferentially binds DNA ends, protecting them from exonucleatic cleavage; likely component of the chromatin-remodeling complex INO80 complex; proposed to be involved in DNA repair Gene:NPP1(YCR026C)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP1 has a paralog, NPP2, that arose from the whole genome duplication Gene:NPR3(YHL023C)|FD-Score:3.21|P-value:6.73E-4||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; required for Npr2p phosphorylation and Iml1p-Npr2p interaction; null mutant shows delayed meiotic DNA replication and double-strand break repair Gene:PDE2(YOR360C)|FD-Score:3.91|P-value:4.56E-5||SGD DESC:High-affinity cyclic AMP phosphodiesterase, component of the cAMP-dependent protein kinase signaling system, protects the cell from extracellular cAMP, contains readthrough motif surrounding termination codon Gene:PDR1(YGL013C)|FD-Score:5.73|P-value:5.14E-9||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PEX12(YMR026C)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder Gene:PMT1(YDL095W)|FD-Score:4.91|P-value:4.56E-7||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose from dolichyl phosphate-D-mannose to protein serine and threonine residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell wall rigidity; involved in ER quality control; amino terminus faces cytoplasm, carboxyl terminus faces ER lumen Gene:POR2(YIL114C)|FD-Score:-3.9|P-value:4.88E-5||SGD DESC:Putative mitochondrial porin (voltage-dependent anion channel); not required for mitochondrial membrane permeability or mitochondrial osmotic stability; POR2 has a paralog, POR1, that arose from the whole genome duplication Gene:PSY3(YLR376C)|FD-Score:4.05|P-value:2.59E-5||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; deletion of PSY3 results in a mutator phenotype; deletion increases sensitivity to anticancer drugs oxaliplatin and cisplatin but not mitomycin C Gene:PYC1(YGL062W)|FD-Score:3.34|P-value:4.20E-4||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc2p; mutations in the human homolog are associated with lactic acidosis; PYC1 has a paralog, PYC2, that arose from the whole genome duplication Gene:RCO1(YMR075W)|FD-Score:4.65|P-value:1.66E-6||SGD DESC:Essential subunit of the histone deacetylase Rpd3S complex; interacts with Eaf3p Gene:RCR2(YDR003W)|FD-Score:-3.35|P-value:3.97E-4||SGD DESC:Vacuolar protein; presumably functions within the endosomal-vacuolar trafficking pathway, affecting events that determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR2 has a paralog, RCR1, that arose from the whole genome duplication Gene:REV1(YOR346W)|FD-Score:3.17|P-value:7.60E-4||SGD DESC:Deoxycytidyl transferase; involved in repair of abasic sites and adducted guanines in damaged DNA by translesion synthesis (TLS); forms a complex with the subunits of DNA polymerase zeta, Rev3p and Rev7p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:REX2(YLR059C)|FD-Score:-3.86|P-value:5.69E-5||SGD DESC:3'-5' RNA exonuclease; involved in 3'-end processing of U4 and U5 snRNAs, 5S and 5.8S rRNAs, and RNase P and RNase MRP RNA; localized to mitochondria and null suppresses escape of mtDNA to nucleus in yme1 yme2 mutants; RNase D exonuclease Gene:RKR1(YMR247C)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:RING domain E3 ubiquitin ligase; involved in the ubiquitin-mediated degradation of non-stop proteins; functional connections to chromatin modification; nuclear protein that also co-localizes with ribosomes; homolog of mouse Listerin, whose mutation has been reported to cause neurodegeneration in mice Gene:RPL4A(YBR031W)|FD-Score:3.23|P-value:6.30E-4||SGD DESC:Ribosomal 60S subunit protein L4A; N-terminally acetylated; homologous to mammalian ribosomal protein L4 and bacterial L4; RPL4A has a paralog, RPL4B, that arose from the whole genome duplication Gene:RTT10(YPL183C)|FD-Score:-3.27|P-value:5.33E-4||SGD DESC:WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6 Gene:SBH1(YER087C-B)|FD-Score:6.26|P-value:1.93E-10||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SIS2(YKR072C)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SMY2(YBR172C)|FD-Score:-3.31|P-value:4.62E-4||SGD DESC:Protein of unknown function involved in COPII vesicle formation; interacts with the Sec23p/Sec24p subcomplex; overexpression suppresses the temperature sensitivity of a myo2 mutant; similar to S. pombe Mpd2; SMY2 has a paralog, SYH1, that arose from the whole genome duplication Gene:SPC72(YAL047C)|FD-Score:3.9|P-value:4.77E-5||SGD DESC:Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization Gene:SPT4(YGR063C)|FD-Score:3.24|P-value:6.08E-4||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:STE13(YOR219C)|FD-Score:3.17|P-value:7.62E-4||SGD DESC:Dipeptidyl aminopeptidase, Golgi integral membrane protein that cleaves on the carboxyl side of repeating -X-Ala- sequences, required for maturation of alpha factor, transcription is induced by a-factor Gene:STP22(YCL008C)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:SUS1(YBR111W-A)|FD-Score:5.14|P-value:1.40E-7||SGD DESC:Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP Gene:TIP41(YPR040W)|FD-Score:4.03|P-value:2.82E-5||SGD DESC:Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress Gene:TIR3(YIL011W)|FD-Score:-3.55|P-value:1.95E-4||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth Gene:UPS3(YDR185C)|FD-Score:5.08|P-value:1.89E-7||SGD DESC:Mitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations Gene:XBP1(YIL101C)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Transcriptional repressor; binds to promoter sequences of the cyclin genes, CYS3, and SMF2; expression is induced by stress or starvation during mitosis, and late in meiosis; member of the Swi4p/Mbp1p family; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:YCR099C(YCR099C_p)|FD-Score:3.32|P-value:4.51E-4||SGD DESC:Putative protein of unknown function Gene:YDR222W(YDR222W_p)|FD-Score:3.32|P-value:4.51E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YER175W-A(YER175W-A_p)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YGR067C(YGR067C_p)|FD-Score:4.31|P-value:8.20E-6||SGD DESC:Putative protein of unknown function; contains a zinc finger motif similar to that of Adr1p Gene:YGR168C(YGR168C_p)|FD-Score:4.26|P-value:1.00E-5||SGD DESC:Putative protein of unknown function; YGR168C is not an essential gene Gene:YHL045W(YHL045W_d)|FD-Score:3.56|P-value:1.82E-4||SGD DESC:Putative protein of unknown function; not an essential gene Gene:YIL012W(YIL012W_d)|FD-Score:-3.26|P-value:5.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIR044C(YIR044C)|FD-Score:-4.33|P-value:7.58E-6||SGD DESC:Possible pseudogene in strain S288C; YIR044C and the adjacent ORF, YIR043C, together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family Gene:YJR084W(YJR084W)|FD-Score:-3.86|P-value:5.69E-5||SGD DESC:Protein that forms a complex with Thp3p; may have a role in transcription elongation and/or mRNA splicing; identified as a COP9 signalosome component but phenotype and interactions suggest it may not be involved with the signalosome Gene:YNL050C(YNL050C_p)|FD-Score:-3.12|P-value:9.02E-4||SGD DESC:Putative protein of unknown function; YNL050c is not an essential gene Gene:YOL014W(YOL014W_p)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Putative protein of unknown function Gene:YOR1(YGR281W)|FD-Score:3.19|P-value:7.07E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter mediates export of many different organic anions including oligomycin; similar to human cystic fibrosis transmembrane receptor (CFTR) Gene:YOR277C(YOR277C_d)|FD-Score:4.32|P-value:7.97E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene CAF20 Gene:YPL071C(YPL071C_p)|FD-Score:-3.38|P-value:3.58E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YPL102C(YPL102C_d)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4 Gene:YPL225W(YPL225W)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YPR109W(YPR109W_p)|FD-Score:3.19|P-value:7.02E-4||SGD DESC:Predicted membrane protein; dipoid deletion strain has high budding index Gene:YPR196W(YPR196W_p)|FD-Score:4.09|P-value:2.16E-5||SGD DESC:Putative maltose-responsive transcription factor Gene:APL6(YGR261C)|FD-Score:4.1|P-value:2.10E-5||SGD DESC:Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools Gene:ASC1(YMR116C)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; regulates P-body formation induced by replication stress; represses Gcn4p in the absence of amino acid starvation Gene:AZF1(YOR113W)|FD-Score:4.62|P-value:1.88E-6||SGD DESC:Zinc-finger transcription factor; in the presence of glucose, activates transcription of genes involved in growth and carbon metabolism; in nonfermentable carbon sources, activates transcription of genes involved in maintenance of cell wall integrity Gene:BOI2(YER114C)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; BOI2 has a paralog, BOI1, that arose from the whole genome duplication Gene:BOR1(YNL275W)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Boron efflux transporter of the plasma membrane; binds HCO3-, I-, Br-, NO3- and Cl-; has similarity to the characterized boron efflux transporter A. thaliana BOR1 Gene:BRO1(YPL084W)|FD-Score:3.73|P-value:9.64E-5||SGD DESC:Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes Gene:CMR3(YPR013C_p)|FD-Score:-3.49|P-value:2.46E-4||SGD DESC:Putative zinc finger protein; YPR013C is not an essential gene Gene:COS10(YNR075W)|FD-Score:-3.27|P-value:5.30E-4||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:CRH1(YGR189C)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar and functionally redundant to Utr2; localizes to sites of polarized growth; expression induced by cell wall stress Gene:DAP1(YPL170W)|FD-Score:-3.12|P-value:9.00E-4||SGD DESC:Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis Gene:ECM30(YLR436C)|FD-Score:4.21|P-value:1.26E-5||SGD DESC:Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants have abormal relative levels of mannose and glucose and have Gap1p sorting and transport defects; (GFP)-fusion protein localizes to the cytoplasm Gene:ELP3(YPL086C)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; exhibits histone acetyltransferase activity that is directed to histones H3 and H4; disruption confers resistance to K. lactis zymotoxin Gene:EPS1(YIL005W)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:ER protein with chaperone and co-chaperone activity, involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family Gene:ERG2(YMR202W)|FD-Score:4.71|P-value:1.26E-6||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ESC1(YMR219W)|FD-Score:3.17|P-value:7.62E-4||SGD DESC:Protein localized to the nuclear periphery, involved in telomeric silencing; interacts with PAD4-domain of Sir4p Gene:FES1(YBR101C)|FD-Score:-3.34|P-value:4.17E-4||SGD DESC:Hsp70 (Ssa1p) nucleotide exchange factor; cytosolic homolog of Sil1p, which is the nucleotide exchange factor for BiP (Kar2p) in the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:FIN1(YDR130C)|FD-Score:-4.9|P-value:4.88E-7||SGD DESC:Spindle pole body-related intermediate filament protein; forms cell cycle-specific filaments between spindle pole bodies in dividing cells; localizes to poles and microtubules of spindle during anaphase and contributes to spindle stability; involved in Glc7p localization and regulation; relative distribution to the nucleus increases upon DNA replication stress Gene:FMP48(YGR052W_p)|FD-Score:4.93|P-value:4.04E-7||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FUB1(YCR076C)|FD-Score:-3.96|P-value:3.78E-5||SGD DESC:Putative protein of unknown function; interacts physically with multiple subunits of the 20S proteasome and genetically with genes encoding 20S core particle and 19S regulatory particle subunits; exhibits boundary activity which blocks the propagation of heterochromatic silencing; contains a PI31 proteasome regulator domain and sequence similarity with human PSMF1, a proteasome inhibitor; not an essential gene Gene:FUN14(YAL008W)|FD-Score:3.15|P-value:8.30E-4||SGD DESC:Mitochondrial protein of unknown function Gene:GPT2(YKR067W)|FD-Score:-3.42|P-value:3.08E-4||SGD DESC:Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen Gene:IRC21(YMR073C)|FD-Score:5.35|P-value:4.42E-8||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:IST2(YBR086C)|FD-Score:3.12|P-value:9.03E-4||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localizes to the mother cell in small-budded cells and to the bud in medium- and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process Gene:LDB19(YOR322C)|FD-Score:4.94|P-value:3.87E-7||SGD DESC:Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye Gene:LOA1(YPR139C)|FD-Score:3.15|P-value:8.04E-4||SGD DESC:Lysophosphatidic acid acyltransferase; involved in triacelglyceride homeostasis and lipid droplet formation; localized to lipid droplets and the ER; specificity for oleoyl-CoA Gene:LYS14(YDR034C)|FD-Score:4.79|P-value:8.47E-7||SGD DESC:Transcriptional activator involved in regulation of genes of the lysine biosynthesis pathway; requires 2-aminoadipate semialdehyde as co-inducer Gene:MAE1(YKL029C)|FD-Score:4.07|P-value:2.33E-5||SGD DESC:Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids Gene:MDM20(YOL076W)|FD-Score:4.88|P-value:5.43E-7||SGD DESC:Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly Gene:MDM36(YPR083W)|FD-Score:4.13|P-value:1.84E-5||SGD DESC:Mitochondrial protein required for normal mitochondrial morphology and inheritance; proposed involvement in the formation of Dnm1p and Num1p-containing cortical anchor complexes that promote mitochondrial fission Gene:MET1(YKR069W)|FD-Score:3.27|P-value:5.37E-4||SGD DESC:S-adenosyl-L-methionine uroporphyrinogen III transmethylase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis Gene:MND2(YIR025W)|FD-Score:4.09|P-value:2.11E-5||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:MRPS35(YGR165W)|FD-Score:-3.25|P-value:5.78E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:NDL1(YLR254C)|FD-Score:4.12|P-value:1.91E-5||SGD DESC:Homolog of nuclear distribution factor NudE, NUDEL; interacts with Pac1p and regulates dynein targeting to microtubule plus ends Gene:NHP10(YDL002C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Protein related to mammalian high mobility group proteins; preferentially binds DNA ends, protecting them from exonucleatic cleavage; likely component of the chromatin-remodeling complex INO80 complex; proposed to be involved in DNA repair Gene:NPP1(YCR026C)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP1 has a paralog, NPP2, that arose from the whole genome duplication Gene:NPR3(YHL023C)|FD-Score:3.21|P-value:6.73E-4||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; required for Npr2p phosphorylation and Iml1p-Npr2p interaction; null mutant shows delayed meiotic DNA replication and double-strand break repair Gene:PDE2(YOR360C)|FD-Score:3.91|P-value:4.56E-5||SGD DESC:High-affinity cyclic AMP phosphodiesterase, component of the cAMP-dependent protein kinase signaling system, protects the cell from extracellular cAMP, contains readthrough motif surrounding termination codon Gene:PDR1(YGL013C)|FD-Score:5.73|P-value:5.14E-9||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PEX12(YMR026C)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder Gene:PMT1(YDL095W)|FD-Score:4.91|P-value:4.56E-7||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose from dolichyl phosphate-D-mannose to protein serine and threonine residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell wall rigidity; involved in ER quality control; amino terminus faces cytoplasm, carboxyl terminus faces ER lumen Gene:POR2(YIL114C)|FD-Score:-3.9|P-value:4.88E-5||SGD DESC:Putative mitochondrial porin (voltage-dependent anion channel); not required for mitochondrial membrane permeability or mitochondrial osmotic stability; POR2 has a paralog, POR1, that arose from the whole genome duplication Gene:PSY3(YLR376C)|FD-Score:4.05|P-value:2.59E-5||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; deletion of PSY3 results in a mutator phenotype; deletion increases sensitivity to anticancer drugs oxaliplatin and cisplatin but not mitomycin C Gene:PYC1(YGL062W)|FD-Score:3.34|P-value:4.20E-4||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc2p; mutations in the human homolog are associated with lactic acidosis; PYC1 has a paralog, PYC2, that arose from the whole genome duplication Gene:RCO1(YMR075W)|FD-Score:4.65|P-value:1.66E-6||SGD DESC:Essential subunit of the histone deacetylase Rpd3S complex; interacts with Eaf3p Gene:RCR2(YDR003W)|FD-Score:-3.35|P-value:3.97E-4||SGD DESC:Vacuolar protein; presumably functions within the endosomal-vacuolar trafficking pathway, affecting events that determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR2 has a paralog, RCR1, that arose from the whole genome duplication Gene:REV1(YOR346W)|FD-Score:3.17|P-value:7.60E-4||SGD DESC:Deoxycytidyl transferase; involved in repair of abasic sites and adducted guanines in damaged DNA by translesion synthesis (TLS); forms a complex with the subunits of DNA polymerase zeta, Rev3p and Rev7p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:REX2(YLR059C)|FD-Score:-3.86|P-value:5.69E-5||SGD DESC:3'-5' RNA exonuclease; involved in 3'-end processing of U4 and U5 snRNAs, 5S and 5.8S rRNAs, and RNase P and RNase MRP RNA; localized to mitochondria and null suppresses escape of mtDNA to nucleus in yme1 yme2 mutants; RNase D exonuclease Gene:RKR1(YMR247C)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:RING domain E3 ubiquitin ligase; involved in the ubiquitin-mediated degradation of non-stop proteins; functional connections to chromatin modification; nuclear protein that also co-localizes with ribosomes; homolog of mouse Listerin, whose mutation has been reported to cause neurodegeneration in mice Gene:RPL4A(YBR031W)|FD-Score:3.23|P-value:6.30E-4||SGD DESC:Ribosomal 60S subunit protein L4A; N-terminally acetylated; homologous to mammalian ribosomal protein L4 and bacterial L4; RPL4A has a paralog, RPL4B, that arose from the whole genome duplication Gene:RTT10(YPL183C)|FD-Score:-3.27|P-value:5.33E-4||SGD DESC:WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6 Gene:SBH1(YER087C-B)|FD-Score:6.26|P-value:1.93E-10||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SIS2(YKR072C)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SMY2(YBR172C)|FD-Score:-3.31|P-value:4.62E-4||SGD DESC:Protein of unknown function involved in COPII vesicle formation; interacts with the Sec23p/Sec24p subcomplex; overexpression suppresses the temperature sensitivity of a myo2 mutant; similar to S. pombe Mpd2; SMY2 has a paralog, SYH1, that arose from the whole genome duplication Gene:SPC72(YAL047C)|FD-Score:3.9|P-value:4.77E-5||SGD DESC:Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization Gene:SPT4(YGR063C)|FD-Score:3.24|P-value:6.08E-4||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:STE13(YOR219C)|FD-Score:3.17|P-value:7.62E-4||SGD DESC:Dipeptidyl aminopeptidase, Golgi integral membrane protein that cleaves on the carboxyl side of repeating -X-Ala- sequences, required for maturation of alpha factor, transcription is induced by a-factor Gene:STP22(YCL008C)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:SUS1(YBR111W-A)|FD-Score:5.14|P-value:1.40E-7||SGD DESC:Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP Gene:TIP41(YPR040W)|FD-Score:4.03|P-value:2.82E-5||SGD DESC:Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress Gene:TIR3(YIL011W)|FD-Score:-3.55|P-value:1.95E-4||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth Gene:UPS3(YDR185C)|FD-Score:5.08|P-value:1.89E-7||SGD DESC:Mitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations Gene:XBP1(YIL101C)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Transcriptional repressor; binds to promoter sequences of the cyclin genes, CYS3, and SMF2; expression is induced by stress or starvation during mitosis, and late in meiosis; member of the Swi4p/Mbp1p family; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:YCR099C(YCR099C_p)|FD-Score:3.32|P-value:4.51E-4||SGD DESC:Putative protein of unknown function Gene:YDR222W(YDR222W_p)|FD-Score:3.32|P-value:4.51E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YER175W-A(YER175W-A_p)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YGR067C(YGR067C_p)|FD-Score:4.31|P-value:8.20E-6||SGD DESC:Putative protein of unknown function; contains a zinc finger motif similar to that of Adr1p Gene:YGR168C(YGR168C_p)|FD-Score:4.26|P-value:1.00E-5||SGD DESC:Putative protein of unknown function; YGR168C is not an essential gene Gene:YHL045W(YHL045W_d)|FD-Score:3.56|P-value:1.82E-4||SGD DESC:Putative protein of unknown function; not an essential gene Gene:YIL012W(YIL012W_d)|FD-Score:-3.26|P-value:5.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIR044C(YIR044C)|FD-Score:-4.33|P-value:7.58E-6||SGD DESC:Possible pseudogene in strain S288C; YIR044C and the adjacent ORF, YIR043C, together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family Gene:YJR084W(YJR084W)|FD-Score:-3.86|P-value:5.69E-5||SGD DESC:Protein that forms a complex with Thp3p; may have a role in transcription elongation and/or mRNA splicing; identified as a COP9 signalosome component but phenotype and interactions suggest it may not be involved with the signalosome Gene:YNL050C(YNL050C_p)|FD-Score:-3.12|P-value:9.02E-4||SGD DESC:Putative protein of unknown function; YNL050c is not an essential gene Gene:YOL014W(YOL014W_p)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Putative protein of unknown function Gene:YOR1(YGR281W)|FD-Score:3.19|P-value:7.07E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter mediates export of many different organic anions including oligomycin; similar to human cystic fibrosis transmembrane receptor (CFTR) Gene:YOR277C(YOR277C_d)|FD-Score:4.32|P-value:7.97E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene CAF20 Gene:YPL071C(YPL071C_p)|FD-Score:-3.38|P-value:3.58E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YPL102C(YPL102C_d)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4 Gene:YPL225W(YPL225W)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YPR109W(YPR109W_p)|FD-Score:3.19|P-value:7.02E-4||SGD DESC:Predicted membrane protein; dipoid deletion strain has high budding index Gene:YPR196W(YPR196W_p)|FD-Score:4.09|P-value:2.16E-5||SGD DESC:Putative maltose-responsive transcription factor

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YER165W5.393.44E-80.83PAB1Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G
YPR025C4.572.48E-60.15CCL1Cyclin associated with protein kinase Kin28p, which is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters
YLR321C4.425.00E-60.68SFH1Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog
YPL235W3.749.17E-50.12RVB2ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly
YOL120C3.621.49E-40.03RPL18ARibosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication
YNL221C3.591.68E-40.33POP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
YBR002C3.265.60E-40.05RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YHR068W3.216.63E-40.05DYS1Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric
YBR004C3.167.93E-40.11GPI18Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria
YPR034W3.050.001160.08ARP7Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YJL009W_d2.970.001500.05YJL009W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex
YBL105C2.920.001760.04PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
YEL034W2.870.002030.10HYP2Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication
YDL209C2.770.002800.04CWC2Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2
YGL113W2.730.003160.01SLD3Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YER087C-B6.261.93E-10SBH1Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p
YGL013C5.735.14E-9PDR1Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication
YMR073C5.354.42E-8IRC21Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study
YBR111W-A5.141.40E-7SUS1Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP
YDR185C5.081.89E-7UPS3Mitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations
YOR322C4.943.87E-7LDB19Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye
YGR052W_p4.934.04E-7FMP48_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation
YDL095W4.914.56E-7PMT1Protein O-mannosyltransferase of the ER membrane; transfers mannose from dolichyl phosphate-D-mannose to protein serine and threonine residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell wall rigidity; involved in ER quality control; amino terminus faces cytoplasm, carboxyl terminus faces ER lumen
YOL076W4.885.43E-7MDM20Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly
YDR034C4.798.47E-7LYS14Transcriptional activator involved in regulation of genes of the lysine biosynthesis pathway; requires 2-aminoadipate semialdehyde as co-inducer
YMR202W4.711.26E-6ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YMR075W4.651.66E-6RCO1Essential subunit of the histone deacetylase Rpd3S complex; interacts with Eaf3p
YOR113W4.621.88E-6AZF1Zinc-finger transcription factor; in the presence of glucose, activates transcription of genes involved in growth and carbon metabolism; in nonfermentable carbon sources, activates transcription of genes involved in maintenance of cell wall integrity
YOR277C_d4.327.97E-6YOR277C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene CAF20
YGR067C_p4.318.20E-6YGR067C_pPutative protein of unknown function; contains a zinc finger motif similar to that of Adr1p

GO enrichment analysis for SGTC_1028
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1201.50E-20SGTC_1826st055655 41.6 μMTimTec (Natural product derivative library)6761680.0759494ERG2
0.1093.60E-17SGTC_8410251-0215 91.2 μMChemDiv (Drug-like library)38886140.0526316
0.1081.09E-16SGTC_1694st024011 44.9 μMTimTec (Natural product derivative library)27875330.0561798ERG2
0.0944.25E-13SGTC_21445628481 200.0 μMChembridge (Fragment library)28183990.0735294ERG2
0.0944.79E-13SGTC_295k015-0027 31.0 μMChemDiv (Drug-like library)38874240.702128PDR1
0.0936.47E-13SGTC_1700st030840 7.9 μMTimTec (Natural product derivative library)54993070.152941PDR1
0.0937.66E-13SGTC_12430416-0003 5.7 μMChemDiv (Drug-like library)21419770.0819672PDR1
0.0921.24E-12SGTC_5274476-4215 268.0 μMChemDiv (Drug-like library)44187540.0860215PDR1
0.0897.22E-12SGTC_1093fluphenazine 8.0 μMNIH Clinical Collection66026110.102564
0.0898.67E-12SGTC_1139k015-0024 46.9 μMChemDiv (Drug-like library)27666520.368421PDR1
0.0898.52E-12SGTC_28629027634 71.4 μMChembridge (Drug-like library)50125620.060241ERG2
0.0881.30E-11SGTC_5983937-0236 20.2 μMChemDiv (Drug-like library)13793130.215385ERG2
0.0814.59E-10SGTC_11831254-0324 198.0 μMChemDiv (Drug-like library)57993570.25
0.0806.58E-10SGTC_12063978-0017 132.0 μMChemDiv (Drug-like library)38736770.0823529ERG2
0.0807.29E-10SGTC_234nsc-17383 10.7 μMMiscellaneous4089740.102564ERG2

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_295k015-002731 μM0.7021283887424ChemDiv (Drug-like library)296.277482.8404PDR1
SGTC_196k015-002318.11 μM0.6326535211998ChemDiv (Drug-like library)361.146963.10204PDR1
SGTC_1457k015-003257.3 μM0.6226424302660ChemDiv (Drug-like library)334.6864233.22405
SGTC_1027k015-00258.67 μM0.5961543598955ChemDiv (Drug-like library)361.146963.10204
SGTC_169k015-001716.03 μM0.4339621840002ChemDiv (Drug-like library)334.1398633.41303
SGTC_349k015-001424 μM0.4181822196908ChemDiv (Drug-like library)350.594463.87202
SGTC_1458k015-00346.33 μM0.4074073598957ChemDiv (Drug-like library)352.1303263.61904
SGTC_23369001819198.65 μM0.42989602Chembridge (Fragment library)209.629021.88902heme biosynthesis & mitochondrial translocase
SGTC_1000086-012849.59 μM0.38888993297ChemDiv (Drug-like library)248.234682.08504copper-dependent oxidative stress
SGTC_1456k015-001213 μM0.3888892989616ChemDiv (Drug-like library)363.149873.03802