k035-0009

ethyl 5-[(5-bromo-2,3-dioxoindol-1-yl)methyl]furan-2-carboxylate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1031
Screen concentration 16.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 5180959
SMILES CCOC(=O)C1=CC=C(O1)CN2C3=C(C=C(C=C3)Br)C(=O)C2=O
Standardized SMILES CCOC(=O)c1oc(CN2C(=O)C(=O)c3cc(Br)ccc23)cc1
Molecular weight 378.1742
ALogP 2.89
H-bond donor count 0
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 7.31
% growth inhibition (Hom. pool) 4.85


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5180959
Download HIP data (tab-delimited text)  (excel)
Gene:GCD10(YNL062C)|FD-Score:-3.67|P-value:1.22E-4|Clearance:0||SGD DESC:Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression Gene:GCD6(YDR211W)|FD-Score:5.9|P-value:1.82E-9|Clearance:2.28||SGD DESC:Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress Gene:GLN1(YPR035W)|FD-Score:-3.15|P-value:8.12E-4|Clearance:0||SGD DESC:Glutamine synthetase (GS); synthesizes glutamine from glutamate and ammonia; with Glt1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source and by amino acid limitation; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:NAM9(YNL137C)|FD-Score:3.11|P-value:9.32E-4|Clearance:0.05||SGD DESC:Mitochondrial ribosomal component of the small subunit Gene:NUP159(YIL115C)|FD-Score:3.17|P-value:7.74E-4|Clearance:0.03||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p) Gene:RPB5(YBR154C)|FD-Score:5.86|P-value:2.29E-9|Clearance:2.28||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RSC4(YKR008W)|FD-Score:3.58|P-value:1.69E-4|Clearance:0.42||SGD DESC:Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p Gene:SEC26(YDR238C)|FD-Score:-3.13|P-value:8.69E-4|Clearance:0||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SPT6(YGR116W)|FD-Score:3.13|P-value:8.60E-4|Clearance:0.02||SGD DESC:Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region Gene:GCD10(YNL062C)|FD-Score:-3.67|P-value:1.22E-4|Clearance:0||SGD DESC:Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression Gene:GCD6(YDR211W)|FD-Score:5.9|P-value:1.82E-9|Clearance:2.28||SGD DESC:Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress Gene:GLN1(YPR035W)|FD-Score:-3.15|P-value:8.12E-4|Clearance:0||SGD DESC:Glutamine synthetase (GS); synthesizes glutamine from glutamate and ammonia; with Glt1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source and by amino acid limitation; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:NAM9(YNL137C)|FD-Score:3.11|P-value:9.32E-4|Clearance:0.05||SGD DESC:Mitochondrial ribosomal component of the small subunit Gene:NUP159(YIL115C)|FD-Score:3.17|P-value:7.74E-4|Clearance:0.03||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p) Gene:RPB5(YBR154C)|FD-Score:5.86|P-value:2.29E-9|Clearance:2.28||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RSC4(YKR008W)|FD-Score:3.58|P-value:1.69E-4|Clearance:0.42||SGD DESC:Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p Gene:SEC26(YDR238C)|FD-Score:-3.13|P-value:8.69E-4|Clearance:0||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SPT6(YGR116W)|FD-Score:3.13|P-value:8.60E-4|Clearance:0.02||SGD DESC:Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5180959
Download HOP data (tab-delimited text)  (excel)
Gene:APS2(YJR058C)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-2, which is involved in protein sorting at the plasma membrane; related to the sigma subunit of the mammalian plasma membrane clathrin-associated protein (AP-2) complex Gene:ASG1(YIL130W)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:Zinc cluster protein proposed to function as a transcriptional regulator involved in the stress response; null mutants have a respiratory deficiency, calcofluor white sensitivity and slightly increased cycloheximide resistance Gene:ATG14(YBR128C)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:Autophagy-specific subunit of phosphatidylinositol 3-kinase complex I (with Vps34/15/30p); Atg14p targets complex I to the phagophore assembly site (PAS); required for localizing additional ATG proteins to the PAS; required for overflow degradation of misfolded proteins when ERAD is saturated; homolog of human Barkor Gene:ATG26(YLR189C)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:UDP-glucose:sterol glucosyltransferase, conserved enzyme involved in synthesis of sterol glucoside membrane lipids; in contrast to ATG26 from P. pastoris, S. cerevisiae ATG26 is not involved in autophagy Gene:AYR1(YIL124W)|FD-Score:3.95|P-value:3.92E-5||SGD DESC:NADPH-dependent 1-acyl dihydroxyacetone phosphate reductase found in lipid particles, ER, and mitochondrial outer membrane; involved in phosphatidic acid biosynthesis; required for spore germination; capable of metabolizing steroid hormones Gene:BNA3(YJL060W)|FD-Score:6.33|P-value:1.20E-10||SGD DESC:Kynurenine aminotransferase, catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate Gene:BTN2(YGR142W)|FD-Score:3.36|P-value:3.88E-4||SGD DESC:v-SNARE binding protein; facilitates specific protein retrieval from a late endosome to the Golgi; modulates arginine uptake, possible role in mediating pH homeostasis between the vacuole and plasma membrane H(+)-ATPase; BTN2 has a paralog, CUR1, that arose from the whole genome duplication Gene:BUD27(YFL023W)|FD-Score:4.05|P-value:2.60E-5||SGD DESC:Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP Gene:CAF120(YNL278W)|FD-Score:3.92|P-value:4.34E-5||SGD DESC:Part of the CCR4-NOT transcriptional regulatory complex; involved in controlling mRNA initiation, elongation, and degradation; CAF120 has a paralog, SKG3, that arose from the whole genome duplication Gene:COG5(YNL051W)|FD-Score:-3.77|P-value:8.23E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:-3.82|P-value:6.73E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COQ3(YOL096C)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:O-methyltransferase, catalyzes two different O-methylation steps in ubiquinone (Coenzyme Q) biosynthesis; component of a mitochondrial ubiquinone-synthesizing complex; phosphoprotein Gene:ENV10(YLR065C)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:FUN30(YAL019W)|FD-Score:6.24|P-value:2.13E-10||SGD DESC:Snf2p family member with ATP-dependent chromatin remodeling activity; has a role in silencing at the mating type locus, telomeres and centromeres; enriched at centromeres and is required for correct chromatin structure around centromeres, as well as at the boundary element of the silent HMR; recruited to DNA double-strand breaks (DSBs) where it promotes 5' strand resection of DSBs; potential Cdc28p substrate Gene:GAT1(YFL021W)|FD-Score:3.1|P-value:9.61E-4||SGD DESC:Transcriptional activator of genes involved in nitrogen catabolite repression; contains a GATA-1-type zinc finger DNA-binding motif; activity and localization regulated by nitrogen limitation and Ure2p Gene:GAT2(YMR136W)|FD-Score:4.19|P-value:1.42E-5||SGD DESC:Protein containing GATA family zinc finger motifs; similar to Gln3p and Dal80p; expression repressed by leucine Gene:GCN2(YDR283C)|FD-Score:3.79|P-value:7.67E-5||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN20(YFR009W)|FD-Score:-3.89|P-value:4.95E-5||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:IRC21(YMR073C)|FD-Score:4.21|P-value:1.25E-5||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:ISN1(YOR155C)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase, catalyzes the breakdown of IMP to inosine, does not show similarity to known 5'-nucleotidases from other organisms Gene:KAR9(YPL269W)|FD-Score:-4.96|P-value:3.58E-7||SGD DESC:Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase Gene:LDH1(YBR204C)|FD-Score:4.03|P-value:2.75E-5||SGD DESC:Serine hydrolase; exhibits active esterase plus weak triacylglycerol lipase activities; proposed role in lipid homeostasis, regulating phospholipid and non-polar lipid levels and required for mobilization of LD-stored lipids; localizes to the lipid droplet (LD) surface; contains a classical serine containing catalytic triad (GxSxG motif) Gene:LHS1(YKL073W)|FD-Score:3.1|P-value:9.62E-4||SGD DESC:Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway Gene:MPP6(YNR024W)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Nuclear exosome-associated RNA binding protein; involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA); copurifies with ribosomes Gene:MRPL31(YKL138C)|FD-Score:5.77|P-value:4.05E-9||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSR1(YHR091C)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Mitochondrial arginyl-tRNA synthetase; mutations in human ortholog are associated with pontocerebellar hypoplasia type 6 Gene:NDJ1(YOL104C)|FD-Score:4.81|P-value:7.50E-7||SGD DESC:Meiosis-specific telomere protein, required for bouquet formation, effective homolog pairing, ordered cross-over distribution, sister chromatid cohesion at meiotic telomeres, chromosomal segregation and telomere-led rapid prophase movement Gene:NUP60(YAR002W)|FD-Score:3.93|P-value:4.31E-5||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; both NUP1 and NUP60 are homologous to human NUP153 Gene:PAL1(YDR348C)|FD-Score:4.27|P-value:9.95E-6||SGD DESC:Protein of unknown function thought to be involved in endocytosis; Pal1p physically interacts with Ede1p and is found at endocytic sites at the cell periphery during the early stages of endocytosis; green fluorescent protein (GFP)-fusion protein also localizes to the bud neck; potential Cdc28p substrate; similar to the S. pombe Pal1 protein; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:PCP1(YGR101W)|FD-Score:-3.18|P-value:7.31E-4||SGD DESC:Mitochondrial serine protease required for the processing of various mitochondrial proteins and maintenance of mitochondrial DNA and morphology; belongs to the rhomboid-GlpG superfamily of intramembrane peptidases Gene:PXA2(YKL188C)|FD-Score:-3.5|P-value:2.32E-4||SGD DESC:Subunit of a heterodimeric peroxisomal ATP-binding cassette transporter complex (Pxa1p-Pxa2p), required for import of long-chain fatty acids into peroxisomes; similarity to human adrenoleukodystrophy transporter ABCD1 and ABCD2 and ALD-related proteins; mutations in ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), a peroxisomal disorder Gene:RAD5(YLR032W)|FD-Score:4.69|P-value:1.37E-6||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:RCF2(YNR018W)|FD-Score:6.31|P-value:1.43E-10||SGD DESC:Cytochrome c oxidase subunit; has a role in assembly of respiratory supercomplexes; similar to Rcf1p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; associates with the cytochrome c oxidase - cytochrome bc1 supercomplex; null mutant accumulates reactive oxygen species; member of the conserved hypoxia induced gene family; C. elegans homolog is functional in yeast Gene:REI1(YBR267W)|FD-Score:5.58|P-value:1.22E-8||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:RIB4(YOL143C)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Lumazine synthase (6,7-dimethyl-8-ribityllumazine synthase, also known as DMRL synthase); catalyzes synthesis of immediate precursor to riboflavin Gene:RIM1(YCR028C-A)|FD-Score:-3.11|P-value:9.28E-4||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:RKR1(YMR247C)|FD-Score:7.01|P-value:1.20E-12||SGD DESC:RING domain E3 ubiquitin ligase; involved in the ubiquitin-mediated degradation of non-stop proteins; functional connections to chromatin modification; nuclear protein that also co-localizes with ribosomes; homolog of mouse Listerin, whose mutation has been reported to cause neurodegeneration in mice Gene:RPL13A(YDL082W)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Ribosomal 60S subunit protein L13A; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13A has a paralog, RPL13B, that arose from the whole genome duplication Gene:RPL19A(YBR084C-A)|FD-Score:3.23|P-value:6.11E-4||SGD DESC:Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication Gene:RPS0B(YLR048W)|FD-Score:3.99|P-value:3.28E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2 Gene:RPS14A(YCR031C)|FD-Score:4.73|P-value:1.10E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication Gene:RTT10(YPL183C)|FD-Score:5.94|P-value:1.45E-9||SGD DESC:WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6 Gene:SAC7(YDR389W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate Gene:SAK1(YER129W)|FD-Score:4.05|P-value:2.52E-5||SGD DESC:Upstream serine/threonine kinase for the SNF1 complex; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome Gene:SFG1(YOR315W)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Nuclear protein, putative transcription factor required for growth of superficial pseudohyphae (which do not invade the agar substrate) but not for invasive pseudohyphal growth; may act together with Phd1p; potential Cdc28p substrate Gene:SIP1(YDR422C)|FD-Score:3.99|P-value:3.33E-5||SGD DESC:Alternate beta-subunit of the Snf1p kinase complex, may confer substrate specificity; vacuolar protein containing KIS (Kinase-Interacting Sequence) and ASC (Association with Snf1 kinase Complex) domains involved in protein interactions Gene:SPC72(YAL047C)|FD-Score:3.37|P-value:3.80E-4||SGD DESC:Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization Gene:SPO7(YAL009W)|FD-Score:3.73|P-value:9.49E-5||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:SPR28(YDR218C)|FD-Score:4.13|P-value:1.85E-5||SGD DESC:Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; meiotic septin expressed at high levels during meiotic divisions and ascospore formation Gene:SPT2(YER161C)|FD-Score:3.3|P-value:4.84E-4||SGD DESC:Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins Gene:SSF1(YHR066W)|FD-Score:3.87|P-value:5.39E-5||SGD DESC:Constituent of 66S pre-ribosomal particles; required for ribosomal large subunit maturation; functionally redundant with Ssf2p; member of the Brix family; SSF1 has a paralog, SSF2, that arose from the whole genome duplication Gene:SSN2(YDR443C)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:SVF1(YDR346C)|FD-Score:-3.8|P-value:7.17E-5||SGD DESC:Protein with a potential role in cell survival pathways, required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance Gene:SYF2(YGR129W)|FD-Score:3.18|P-value:7.43E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:TMT1(YER175C)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:Trans-aconitate methyltransferase, cytosolic enzyme that catalyzes the methyl esterification of 3-isopropylmalate, an intermediate of the leucine biosynthetic pathway, and trans-aconitate, which inhibits the citric acid cycle Gene:TOM1(YDR457W)|FD-Score:3.74|P-value:9.22E-5||SGD DESC:E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:UGA2(YBR006W)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:URA7(YBL039C)|FD-Score:4.03|P-value:2.82E-5||SGD DESC:Major CTP synthase isozyme (see also URA8); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA7 has a paralog, URA8, that arose from the whole genome duplication Gene:VPS1(YKR001C)|FD-Score:-4.19|P-value:1.37E-5||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:YBL012C(YBL012C_d)|FD-Score:3.14|P-value:8.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR071W(YBR071W)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:Protein of unknown function found in the cytoplasm and bud neck; mRNA expression may be regulated by the cell cycle and/or cell wall stress; overexpression of YBR071W affects endocytic protein trafficking Gene:YBR259W(YBR259W_p)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Protein of unknown function; YBR259W is not an essential gene; forms cytoplasmic foci upon DNA replication stress Gene:YBR277C(YBR277C_d)|FD-Score:4.88|P-value:5.40E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W Gene:YCK2(YNL154C)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Palmitoylated plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck1p in morphogenesis, proper septin assembly, endocytic trafficking; YCK2 has a paralog, YCK1, that arose from the whole genome duplication Gene:YEL028W(YEL028W_d)|FD-Score:4.22|P-value:1.20E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YEL045C(YEL045C_d)|FD-Score:-4.98|P-value:3.13E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress Gene:YER097W(YER097W_d)|FD-Score:4.04|P-value:2.70E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL034C(YGL034C_d)|FD-Score:3.17|P-value:7.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLL007C(YLL007C_p)|FD-Score:-3.14|P-value:8.59E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YLR040C(YLR040C_p)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Protein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; mRNA identified as translated by ribosome profiling data; YLR040C is not essential Gene:YLR444C(YLR444C_d)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML009C-A(YML009C-A_d)|FD-Score:4.92|P-value:4.23E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML012C-A(YML012C-A_d)|FD-Score:6.13|P-value:4.36E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YMR090W(YMR090W_p)|FD-Score:4.4|P-value:5.53E-6||SGD DESC:Putative protein of unknown function with similarity to DTDP-glucose 4,6-dehydratases; GFP-fusion protein localizes to the cytoplasm; up-regulated in response to the fungicide mancozeb; not essential for viability Gene:YMR196W(YMR196W_p)|FD-Score:-3.2|P-value:6.81E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene Gene:YNL034W(YNL034W_p)|FD-Score:3.98|P-value:3.49E-5||SGD DESC:Putative protein of unknown function; YNL034W is not an essential gene Gene:YOL107W(YOL107W_p)|FD-Score:6.99|P-value:1.34E-12||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and colocalizes in a punctate pattern with the early golgi/COPI vesicles; YOL107W is not an essential protein Gene:YOR105W(YOR105W_p)|FD-Score:4.09|P-value:2.19E-5||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOR121C(YOR121C_d)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GCY1/YOR120W Gene:YOR378W(YOR378W_p)|FD-Score:3.29|P-value:5.07E-4||SGD DESC:Putative paralog of ATR1, but not required for boron tolerance; member of the DHA2 family of drug:H+ antiporters; YOR378W is not an essential gene Gene:YPL071C(YPL071C_p)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YPL102C(YPL102C_d)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4 Gene:YPL168W(YPL168W_p)|FD-Score:-4.18|P-value:1.45E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; expression may be cell cycle-regulated Gene:YPR123C(YPR123C_d)|FD-Score:4.04|P-value:2.71E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPT53(YNL093W)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication Gene:APS2(YJR058C)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-2, which is involved in protein sorting at the plasma membrane; related to the sigma subunit of the mammalian plasma membrane clathrin-associated protein (AP-2) complex Gene:ASG1(YIL130W)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:Zinc cluster protein proposed to function as a transcriptional regulator involved in the stress response; null mutants have a respiratory deficiency, calcofluor white sensitivity and slightly increased cycloheximide resistance Gene:ATG14(YBR128C)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:Autophagy-specific subunit of phosphatidylinositol 3-kinase complex I (with Vps34/15/30p); Atg14p targets complex I to the phagophore assembly site (PAS); required for localizing additional ATG proteins to the PAS; required for overflow degradation of misfolded proteins when ERAD is saturated; homolog of human Barkor Gene:ATG26(YLR189C)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:UDP-glucose:sterol glucosyltransferase, conserved enzyme involved in synthesis of sterol glucoside membrane lipids; in contrast to ATG26 from P. pastoris, S. cerevisiae ATG26 is not involved in autophagy Gene:AYR1(YIL124W)|FD-Score:3.95|P-value:3.92E-5||SGD DESC:NADPH-dependent 1-acyl dihydroxyacetone phosphate reductase found in lipid particles, ER, and mitochondrial outer membrane; involved in phosphatidic acid biosynthesis; required for spore germination; capable of metabolizing steroid hormones Gene:BNA3(YJL060W)|FD-Score:6.33|P-value:1.20E-10||SGD DESC:Kynurenine aminotransferase, catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate Gene:BTN2(YGR142W)|FD-Score:3.36|P-value:3.88E-4||SGD DESC:v-SNARE binding protein; facilitates specific protein retrieval from a late endosome to the Golgi; modulates arginine uptake, possible role in mediating pH homeostasis between the vacuole and plasma membrane H(+)-ATPase; BTN2 has a paralog, CUR1, that arose from the whole genome duplication Gene:BUD27(YFL023W)|FD-Score:4.05|P-value:2.60E-5||SGD DESC:Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP Gene:CAF120(YNL278W)|FD-Score:3.92|P-value:4.34E-5||SGD DESC:Part of the CCR4-NOT transcriptional regulatory complex; involved in controlling mRNA initiation, elongation, and degradation; CAF120 has a paralog, SKG3, that arose from the whole genome duplication Gene:COG5(YNL051W)|FD-Score:-3.77|P-value:8.23E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:-3.82|P-value:6.73E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COQ3(YOL096C)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:O-methyltransferase, catalyzes two different O-methylation steps in ubiquinone (Coenzyme Q) biosynthesis; component of a mitochondrial ubiquinone-synthesizing complex; phosphoprotein Gene:ENV10(YLR065C)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:FUN30(YAL019W)|FD-Score:6.24|P-value:2.13E-10||SGD DESC:Snf2p family member with ATP-dependent chromatin remodeling activity; has a role in silencing at the mating type locus, telomeres and centromeres; enriched at centromeres and is required for correct chromatin structure around centromeres, as well as at the boundary element of the silent HMR; recruited to DNA double-strand breaks (DSBs) where it promotes 5' strand resection of DSBs; potential Cdc28p substrate Gene:GAT1(YFL021W)|FD-Score:3.1|P-value:9.61E-4||SGD DESC:Transcriptional activator of genes involved in nitrogen catabolite repression; contains a GATA-1-type zinc finger DNA-binding motif; activity and localization regulated by nitrogen limitation and Ure2p Gene:GAT2(YMR136W)|FD-Score:4.19|P-value:1.42E-5||SGD DESC:Protein containing GATA family zinc finger motifs; similar to Gln3p and Dal80p; expression repressed by leucine Gene:GCN2(YDR283C)|FD-Score:3.79|P-value:7.67E-5||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN20(YFR009W)|FD-Score:-3.89|P-value:4.95E-5||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:IRC21(YMR073C)|FD-Score:4.21|P-value:1.25E-5||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:ISN1(YOR155C)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase, catalyzes the breakdown of IMP to inosine, does not show similarity to known 5'-nucleotidases from other organisms Gene:KAR9(YPL269W)|FD-Score:-4.96|P-value:3.58E-7||SGD DESC:Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase Gene:LDH1(YBR204C)|FD-Score:4.03|P-value:2.75E-5||SGD DESC:Serine hydrolase; exhibits active esterase plus weak triacylglycerol lipase activities; proposed role in lipid homeostasis, regulating phospholipid and non-polar lipid levels and required for mobilization of LD-stored lipids; localizes to the lipid droplet (LD) surface; contains a classical serine containing catalytic triad (GxSxG motif) Gene:LHS1(YKL073W)|FD-Score:3.1|P-value:9.62E-4||SGD DESC:Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway Gene:MPP6(YNR024W)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Nuclear exosome-associated RNA binding protein; involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA); copurifies with ribosomes Gene:MRPL31(YKL138C)|FD-Score:5.77|P-value:4.05E-9||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSR1(YHR091C)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Mitochondrial arginyl-tRNA synthetase; mutations in human ortholog are associated with pontocerebellar hypoplasia type 6 Gene:NDJ1(YOL104C)|FD-Score:4.81|P-value:7.50E-7||SGD DESC:Meiosis-specific telomere protein, required for bouquet formation, effective homolog pairing, ordered cross-over distribution, sister chromatid cohesion at meiotic telomeres, chromosomal segregation and telomere-led rapid prophase movement Gene:NUP60(YAR002W)|FD-Score:3.93|P-value:4.31E-5||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; both NUP1 and NUP60 are homologous to human NUP153 Gene:PAL1(YDR348C)|FD-Score:4.27|P-value:9.95E-6||SGD DESC:Protein of unknown function thought to be involved in endocytosis; Pal1p physically interacts with Ede1p and is found at endocytic sites at the cell periphery during the early stages of endocytosis; green fluorescent protein (GFP)-fusion protein also localizes to the bud neck; potential Cdc28p substrate; similar to the S. pombe Pal1 protein; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:PCP1(YGR101W)|FD-Score:-3.18|P-value:7.31E-4||SGD DESC:Mitochondrial serine protease required for the processing of various mitochondrial proteins and maintenance of mitochondrial DNA and morphology; belongs to the rhomboid-GlpG superfamily of intramembrane peptidases Gene:PXA2(YKL188C)|FD-Score:-3.5|P-value:2.32E-4||SGD DESC:Subunit of a heterodimeric peroxisomal ATP-binding cassette transporter complex (Pxa1p-Pxa2p), required for import of long-chain fatty acids into peroxisomes; similarity to human adrenoleukodystrophy transporter ABCD1 and ABCD2 and ALD-related proteins; mutations in ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), a peroxisomal disorder Gene:RAD5(YLR032W)|FD-Score:4.69|P-value:1.37E-6||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:RCF2(YNR018W)|FD-Score:6.31|P-value:1.43E-10||SGD DESC:Cytochrome c oxidase subunit; has a role in assembly of respiratory supercomplexes; similar to Rcf1p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; associates with the cytochrome c oxidase - cytochrome bc1 supercomplex; null mutant accumulates reactive oxygen species; member of the conserved hypoxia induced gene family; C. elegans homolog is functional in yeast Gene:REI1(YBR267W)|FD-Score:5.58|P-value:1.22E-8||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:RIB4(YOL143C)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Lumazine synthase (6,7-dimethyl-8-ribityllumazine synthase, also known as DMRL synthase); catalyzes synthesis of immediate precursor to riboflavin Gene:RIM1(YCR028C-A)|FD-Score:-3.11|P-value:9.28E-4||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:RKR1(YMR247C)|FD-Score:7.01|P-value:1.20E-12||SGD DESC:RING domain E3 ubiquitin ligase; involved in the ubiquitin-mediated degradation of non-stop proteins; functional connections to chromatin modification; nuclear protein that also co-localizes with ribosomes; homolog of mouse Listerin, whose mutation has been reported to cause neurodegeneration in mice Gene:RPL13A(YDL082W)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Ribosomal 60S subunit protein L13A; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13A has a paralog, RPL13B, that arose from the whole genome duplication Gene:RPL19A(YBR084C-A)|FD-Score:3.23|P-value:6.11E-4||SGD DESC:Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication Gene:RPS0B(YLR048W)|FD-Score:3.99|P-value:3.28E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2 Gene:RPS14A(YCR031C)|FD-Score:4.73|P-value:1.10E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication Gene:RTT10(YPL183C)|FD-Score:5.94|P-value:1.45E-9||SGD DESC:WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6 Gene:SAC7(YDR389W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate Gene:SAK1(YER129W)|FD-Score:4.05|P-value:2.52E-5||SGD DESC:Upstream serine/threonine kinase for the SNF1 complex; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome Gene:SFG1(YOR315W)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Nuclear protein, putative transcription factor required for growth of superficial pseudohyphae (which do not invade the agar substrate) but not for invasive pseudohyphal growth; may act together with Phd1p; potential Cdc28p substrate Gene:SIP1(YDR422C)|FD-Score:3.99|P-value:3.33E-5||SGD DESC:Alternate beta-subunit of the Snf1p kinase complex, may confer substrate specificity; vacuolar protein containing KIS (Kinase-Interacting Sequence) and ASC (Association with Snf1 kinase Complex) domains involved in protein interactions Gene:SPC72(YAL047C)|FD-Score:3.37|P-value:3.80E-4||SGD DESC:Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization Gene:SPO7(YAL009W)|FD-Score:3.73|P-value:9.49E-5||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:SPR28(YDR218C)|FD-Score:4.13|P-value:1.85E-5||SGD DESC:Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; meiotic septin expressed at high levels during meiotic divisions and ascospore formation Gene:SPT2(YER161C)|FD-Score:3.3|P-value:4.84E-4||SGD DESC:Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins Gene:SSF1(YHR066W)|FD-Score:3.87|P-value:5.39E-5||SGD DESC:Constituent of 66S pre-ribosomal particles; required for ribosomal large subunit maturation; functionally redundant with Ssf2p; member of the Brix family; SSF1 has a paralog, SSF2, that arose from the whole genome duplication Gene:SSN2(YDR443C)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:SVF1(YDR346C)|FD-Score:-3.8|P-value:7.17E-5||SGD DESC:Protein with a potential role in cell survival pathways, required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance Gene:SYF2(YGR129W)|FD-Score:3.18|P-value:7.43E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:TMT1(YER175C)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:Trans-aconitate methyltransferase, cytosolic enzyme that catalyzes the methyl esterification of 3-isopropylmalate, an intermediate of the leucine biosynthetic pathway, and trans-aconitate, which inhibits the citric acid cycle Gene:TOM1(YDR457W)|FD-Score:3.74|P-value:9.22E-5||SGD DESC:E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:UGA2(YBR006W)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:URA7(YBL039C)|FD-Score:4.03|P-value:2.82E-5||SGD DESC:Major CTP synthase isozyme (see also URA8); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA7 has a paralog, URA8, that arose from the whole genome duplication Gene:VPS1(YKR001C)|FD-Score:-4.19|P-value:1.37E-5||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:YBL012C(YBL012C_d)|FD-Score:3.14|P-value:8.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR071W(YBR071W)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:Protein of unknown function found in the cytoplasm and bud neck; mRNA expression may be regulated by the cell cycle and/or cell wall stress; overexpression of YBR071W affects endocytic protein trafficking Gene:YBR259W(YBR259W_p)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Protein of unknown function; YBR259W is not an essential gene; forms cytoplasmic foci upon DNA replication stress Gene:YBR277C(YBR277C_d)|FD-Score:4.88|P-value:5.40E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W Gene:YCK2(YNL154C)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Palmitoylated plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck1p in morphogenesis, proper septin assembly, endocytic trafficking; YCK2 has a paralog, YCK1, that arose from the whole genome duplication Gene:YEL028W(YEL028W_d)|FD-Score:4.22|P-value:1.20E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YEL045C(YEL045C_d)|FD-Score:-4.98|P-value:3.13E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress Gene:YER097W(YER097W_d)|FD-Score:4.04|P-value:2.70E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL034C(YGL034C_d)|FD-Score:3.17|P-value:7.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLL007C(YLL007C_p)|FD-Score:-3.14|P-value:8.59E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YLR040C(YLR040C_p)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Protein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; mRNA identified as translated by ribosome profiling data; YLR040C is not essential Gene:YLR444C(YLR444C_d)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML009C-A(YML009C-A_d)|FD-Score:4.92|P-value:4.23E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML012C-A(YML012C-A_d)|FD-Score:6.13|P-value:4.36E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YMR090W(YMR090W_p)|FD-Score:4.4|P-value:5.53E-6||SGD DESC:Putative protein of unknown function with similarity to DTDP-glucose 4,6-dehydratases; GFP-fusion protein localizes to the cytoplasm; up-regulated in response to the fungicide mancozeb; not essential for viability Gene:YMR196W(YMR196W_p)|FD-Score:-3.2|P-value:6.81E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene Gene:YNL034W(YNL034W_p)|FD-Score:3.98|P-value:3.49E-5||SGD DESC:Putative protein of unknown function; YNL034W is not an essential gene Gene:YOL107W(YOL107W_p)|FD-Score:6.99|P-value:1.34E-12||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and colocalizes in a punctate pattern with the early golgi/COPI vesicles; YOL107W is not an essential protein Gene:YOR105W(YOR105W_p)|FD-Score:4.09|P-value:2.19E-5||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOR121C(YOR121C_d)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GCY1/YOR120W Gene:YOR378W(YOR378W_p)|FD-Score:3.29|P-value:5.07E-4||SGD DESC:Putative paralog of ATR1, but not required for boron tolerance; member of the DHA2 family of drug:H+ antiporters; YOR378W is not an essential gene Gene:YPL071C(YPL071C_p)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YPL102C(YPL102C_d)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4 Gene:YPL168W(YPL168W_p)|FD-Score:-4.18|P-value:1.45E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; expression may be cell cycle-regulated Gene:YPR123C(YPR123C_d)|FD-Score:4.04|P-value:2.71E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPT53(YNL093W)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR211W5.901.82E-92.28GCD6Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress
YBR154C5.862.29E-92.28RPB5RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation
YKR008W3.581.69E-40.42RSC4Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p
YIL115C3.177.74E-40.03NUP159FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p)
YGR116W3.138.60E-40.02SPT6Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region
YNL137C3.119.32E-40.05NAM9Mitochondrial ribosomal component of the small subunit
YER133W3.060.001100.09GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YIL062C2.970.001480.07ARC15Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; has mRNA binding activity
YBL023C2.900.001870.01MCM2Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress
YGL137W2.890.001920.04SEC27Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP
YNL258C2.850.002170.05DSL1Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; forms a complex with Sec39p and Tip20p that interacts with ER SNAREs Sec20p and Use1p; component of the ER target site that interacts with coatomer; interacts with Cin5p; similar to the fly and human ZW10 gene
YBR109C2.800.002540.00CMD1Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin
YBL026W2.800.002580.07LSM2Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YKL042W2.730.003180.04SPC42Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane
YFR029W2.690.003530.05PTR3Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR247C7.011.20E-12RKR1RING domain E3 ubiquitin ligase; involved in the ubiquitin-mediated degradation of non-stop proteins; functional connections to chromatin modification; nuclear protein that also co-localizes with ribosomes; homolog of mouse Listerin, whose mutation has been reported to cause neurodegeneration in mice
YOL107W_p6.991.34E-12YOL107W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and colocalizes in a punctate pattern with the early golgi/COPI vesicles; YOL107W is not an essential protein
YJL060W6.331.20E-10BNA3Kynurenine aminotransferase, catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate
YNR018W6.311.43E-10RCF2Cytochrome c oxidase subunit; has a role in assembly of respiratory supercomplexes; similar to Rcf1p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; associates with the cytochrome c oxidase - cytochrome bc1 supercomplex; null mutant accumulates reactive oxygen species; member of the conserved hypoxia induced gene family; C. elegans homolog is functional in yeast
YAL019W6.242.13E-10FUN30Snf2p family member with ATP-dependent chromatin remodeling activity; has a role in silencing at the mating type locus, telomeres and centromeres; enriched at centromeres and is required for correct chromatin structure around centromeres, as well as at the boundary element of the silent HMR; recruited to DNA double-strand breaks (DSBs) where it promotes 5' strand resection of DSBs; potential Cdc28p substrate
YML012C-A_d6.134.36E-10YML012C-A_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1
YPL183C5.941.45E-9RTT10WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6
YKL138C5.774.05E-9MRPL31Mitochondrial ribosomal protein of the large subunit
YBR267W5.581.22E-8REI1Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network
YML009C-A_d4.924.23E-7YML009C-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YBR277C_d4.885.40E-7YBR277C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W
YOL104C4.817.50E-7NDJ1Meiosis-specific telomere protein, required for bouquet formation, effective homolog pairing, ordered cross-over distribution, sister chromatid cohesion at meiotic telomeres, chromosomal segregation and telomere-led rapid prophase movement
YCR031C4.731.10E-6RPS14AProtein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication
YLR032W4.691.37E-6RAD5DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress
YMR090W_p4.405.53E-6YMR090W_pPutative protein of unknown function with similarity to DTDP-glucose 4,6-dehydratases; GFP-fusion protein localizes to the cytoplasm; up-regulated in response to the fungicide mancozeb; not essential for viability

GO enrichment analysis for SGTC_1031
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0814.69E-10SGTC_620180-0423 3.2 μMChemDiv (Drug-like library)301020.12658260S ribosome export
0.0672.73E-7SGTC_21715739187 198.1 μMChembridge (Fragment library)22627610.116667copper-dependent oxidative stress
0.0604.47E-6SGTC_2598perillyl alcohol 100.0 μMMicrosource (Natural product library)108190.0714286
0.0595.78E-6SGTC_8690564-0080 44.1 μMChemDiv (Drug-like library)68433210.0540541
0.0543.13E-5SGTC_32099113616 49.5 μMChembridge (Drug-like library)166493530.104651
0.0543.21E-5SGTC_22016605497 169.9 μMChembridge (Fragment library)6707410.132353ergosterol biosynthesis
0.0525.61E-5SGTC_8610443-0273 110.0 μMChemDiv (Drug-like library)61491690.0519481
0.0526.90E-5SGTC_712r015-0009 80.6 μMChemDiv (Drug-like library)68138720.0833333
0.0527.01E-5SGTC_2130NSC 7 2.5 μMChembridge (Fragment library)214480.126984copper-dependent oxidative stress
0.0519.00E-5SGTC_20575236571 38.3 μMChembridge (Fragment library)54684039NARPP1 & pyrimidine depletion
0.0501.11E-4SGTC_1893st059353 36.1 μMTimTec (Natural product derivative library)31124880.115789
0.0491.42E-4SGTC_30309087619 49.5 μMChembridge (Drug-like library)252369730.157143
0.0482.48E-4SGTC_20104012422 159.0 μMChembridge (Fragment library)9523930.0714286
0.0472.65E-4SGTC_608k048-0125 159.0 μMChemDiv (Drug-like library)68176520.181818
0.0472.77E-4SGTC_7041310-0270 142.0 μMChemDiv (Drug-like library)57180330.234375

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_169k015-001716.03 μM0.4642861840002ChemDiv (Drug-like library)334.1398633.41303
SGTC_1458k015-00346.33 μM0.4642863598957ChemDiv (Drug-like library)352.1303263.61904
SGTC_349k015-001424 μM0.4482762196908ChemDiv (Drug-like library)350.594463.87202
SGTC_10483161-030024.5 μM0.4363642165101ChemDiv (Drug-like library)266.090722.24102
SGTC_196k015-002318.11 μM0.4333335211998ChemDiv (Drug-like library)361.146963.10204PDR1
SGTC_1027k015-00258.67 μM0.4126983598955ChemDiv (Drug-like library)361.146963.10204
SGTC_11750741-005627.6 μM0.384615765927ChemDiv (Drug-like library)318.321223.61826
SGTC_12620737-011449.6 μM0.373134765919ChemDiv (Drug-like library)332.34784.07426
SGTC_1871st05744867.3 μM0.370968691747TimTec (Natural product derivative library)297.101462.81204
SGTC_544k015-002052.4 μM0.3684213008304ChemDiv (Drug-like library)316.14943.20802