k061-1018

N-[3-(4-ethoxyphenoxy)-5-[[2-(2-methylphenoxy)acetyl]amino]phenyl]-2-(2-methylphenoxy)acetamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1032
Screen concentration 98.7 μM
Source ChemDiv (Drug-like library)
PubChem CID 4005393
SMILES CCOC1=CC=C(C=C1)OC2=CC(=CC(=C2)NC(=O)COC3=CC=CC=C3C)NC(=O)COC4=CC=CC=C4C
Standardized SMILES CCOc1ccc(Oc2cc(NC(=O)COc3ccccc3C)cc(NC(=O)COc4ccccc4C)c2)cc1
Molecular weight 540.6063
ALogP 5.94
H-bond donor count 2
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 1.03
% growth inhibition (Hom. pool) 1.27


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4005393
Download HIP data (tab-delimited text)  (excel)
Gene:DBP2(YNL112W)|FD-Score:3.62|P-value:1.45E-4|Clearance:0||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:ERG9(YHR190W)|FD-Score:-3.51|P-value:2.26E-4|Clearance:0||SGD DESC:Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway Gene:GPI10(YGL142C)|FD-Score:3.61|P-value:1.55E-4|Clearance:0.01||SGD DESC:Integral membrane protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; putative alpha 1,2 mannosyltransferase required for addition of the third mannose onto the GPI core structure; human PIG-Bp is a functional homolog Gene:KRI1(YNL308C)|FD-Score:4.23|P-value:1.14E-5|Clearance:0.41||SGD DESC:Essential nucleolar protein required for 40S ribosome biogenesis; associate with snR30; physically and functionally interacts with Krr1p Gene:MET30(YIL046W)|FD-Score:3.73|P-value:9.55E-5|Clearance:0.11||SGD DESC:F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus Gene:NOP15(YNL110C)|FD-Score:4.22|P-value:1.23E-5|Clearance:0.41||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:NOP56(YLR197W)|FD-Score:3.77|P-value:8.03E-5|Clearance:0.04||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:PGK1(YCR012W)|FD-Score:3.6|P-value:1.58E-4|Clearance:0.22||SGD DESC:3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phosphate of 1,3-bisphosphoglycerate to ADP to produce ATP; key enzyme in glycolysis and gluconeogenesis Gene:RPF1(YHR088W)|FD-Score:3.38|P-value:3.57E-4|Clearance:0.1||SGD DESC:Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA Gene:SCC2(YDR180W)|FD-Score:-3.18|P-value:7.27E-4|Clearance:0||SGD DESC:Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; evolutionarily-conserved adherin Gene:SKP1(YDR328C)|FD-Score:3.62|P-value:1.45E-4|Clearance:0.02||SGD DESC:Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress Gene:SNP1(YIL061C)|FD-Score:3.28|P-value:5.14E-4|Clearance:0.08||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress Gene:SNU71(YGR013W)|FD-Score:4.46|P-value:4.07E-6|Clearance:0.41||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart Gene:TRL1(YJL087C)|FD-Score:3.2|P-value:6.78E-4|Clearance:0.23||SGD DESC:tRNA ligase, required for tRNA splicing and for both splicing and translation of HAC1 mRNA in the UPR; has phosphodiesterase, polynucleotide kinase, and ligase activities; localized at the inner nuclear envelope and partially to polysomes Gene:YBR190W(YBR190W_d)|FD-Score:4.19|P-value:1.41E-5|Clearance:0.41||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Gene:YOR146W(YOR146W_d)|FD-Score:4.48|P-value:3.77E-6|Clearance:0.41||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C Gene:DBP2(YNL112W)|FD-Score:3.62|P-value:1.45E-4|Clearance:0||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:ERG9(YHR190W)|FD-Score:-3.51|P-value:2.26E-4|Clearance:0||SGD DESC:Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway Gene:GPI10(YGL142C)|FD-Score:3.61|P-value:1.55E-4|Clearance:0.01||SGD DESC:Integral membrane protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; putative alpha 1,2 mannosyltransferase required for addition of the third mannose onto the GPI core structure; human PIG-Bp is a functional homolog Gene:KRI1(YNL308C)|FD-Score:4.23|P-value:1.14E-5|Clearance:0.41||SGD DESC:Essential nucleolar protein required for 40S ribosome biogenesis; associate with snR30; physically and functionally interacts with Krr1p Gene:MET30(YIL046W)|FD-Score:3.73|P-value:9.55E-5|Clearance:0.11||SGD DESC:F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus Gene:NOP15(YNL110C)|FD-Score:4.22|P-value:1.23E-5|Clearance:0.41||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:NOP56(YLR197W)|FD-Score:3.77|P-value:8.03E-5|Clearance:0.04||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:PGK1(YCR012W)|FD-Score:3.6|P-value:1.58E-4|Clearance:0.22||SGD DESC:3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phosphate of 1,3-bisphosphoglycerate to ADP to produce ATP; key enzyme in glycolysis and gluconeogenesis Gene:RPF1(YHR088W)|FD-Score:3.38|P-value:3.57E-4|Clearance:0.1||SGD DESC:Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA Gene:SCC2(YDR180W)|FD-Score:-3.18|P-value:7.27E-4|Clearance:0||SGD DESC:Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; evolutionarily-conserved adherin Gene:SKP1(YDR328C)|FD-Score:3.62|P-value:1.45E-4|Clearance:0.02||SGD DESC:Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress Gene:SNP1(YIL061C)|FD-Score:3.28|P-value:5.14E-4|Clearance:0.08||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress Gene:SNU71(YGR013W)|FD-Score:4.46|P-value:4.07E-6|Clearance:0.41||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart Gene:TRL1(YJL087C)|FD-Score:3.2|P-value:6.78E-4|Clearance:0.23||SGD DESC:tRNA ligase, required for tRNA splicing and for both splicing and translation of HAC1 mRNA in the UPR; has phosphodiesterase, polynucleotide kinase, and ligase activities; localized at the inner nuclear envelope and partially to polysomes Gene:YBR190W(YBR190W_d)|FD-Score:4.19|P-value:1.41E-5|Clearance:0.41||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Gene:YOR146W(YOR146W_d)|FD-Score:4.48|P-value:3.77E-6|Clearance:0.41||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4005393
Download HOP data (tab-delimited text)  (excel)
Gene:ANT1(YPR128C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation Gene:AYR1(YIL124W)|FD-Score:3.48|P-value:2.46E-4||SGD DESC:NADPH-dependent 1-acyl dihydroxyacetone phosphate reductase found in lipid particles, ER, and mitochondrial outer membrane; involved in phosphatidic acid biosynthesis; required for spore germination; capable of metabolizing steroid hormones Gene:CAM1(YPL048W)|FD-Score:5.12|P-value:1.52E-7||SGD DESC:Nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids; has similarity to translational cofactor EF-1 gamma Gene:CAT5(YOR125C)|FD-Score:-3.96|P-value:3.82E-5||SGD DESC:Protein required for ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes; required for gluconeogenic gene activation Gene:CBR1(YIL043C)|FD-Score:3.82|P-value:6.65E-5||SGD DESC:Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia Gene:CCC2(YDR270W)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism Gene:COQ10(YOL008W)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q<sub>6</sub> to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes Gene:CTF3(YLR381W)|FD-Score:3.84|P-value:6.20E-5||SGD DESC:Outer kinetochore protein that forms a complex with Mcm16p and Mcm22p; may bind the kinetochore to spindle microtubules; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-I and fission yeast mis6 Gene:ECM38(YLR299W)|FD-Score:3.3|P-value:4.88E-4||SGD DESC:Gamma-glutamyltranspeptidase, major glutathione-degrading enzyme; involved in detoxification of electrophilic xenobiotics; expression induced mainly by nitrogen starvation Gene:EMC4(YGL231C)|FD-Score:-4.11|P-value:1.96E-5||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4, fly CG11137, human TMM85 Gene:EPS1(YIL005W)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:ER protein with chaperone and co-chaperone activity, involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family Gene:ERD1(YDR414C)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p) Gene:ERG4(YGL012W)|FD-Score:4.09|P-value:2.16E-5||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FIR1(YER032W)|FD-Score:3.53|P-value:2.12E-4||SGD DESC:Protein involved in 3' mRNA processing, interacts with Ref2p; potential Cdc28p substrate Gene:KAP104(YBR017C)|FD-Score:3.78|P-value:7.85E-5||SGD DESC:Transportin or cytosolic karyopherin beta 2; functions in the rg-nuclear localization signal-mediated nuclear import/reimport of mRNA-binding proteins Nab2p and Hrp1p; regulates asymmetric protein synthesis in daughter cells during mitosis Gene:LCB4(YOR171C)|FD-Score:3.77|P-value:8.30E-5||SGD DESC:Sphingoid long-chain base kinase, responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes Gene:MAG2(YLR427W)|FD-Score:-3.53|P-value:2.08E-4||SGD DESC:Cytoplasmic protein of unknown function; induced in response to mycotoxin patulin; ubiquitinated protein similar to the human ring finger motif protein RNF10; predicted to be involved in repair of alkylated DNA due to interaction with MAG1 Gene:MDH2(YOL126C)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Cytoplasmic malate dehydrogenase, one of three isozymes that catalyze interconversion of malate and oxaloacetate; involved in the glyoxylate cycle and gluconeogenesis during growth on two-carbon compounds; interacts with Pck1p and Fbp1 Gene:MNN2(YBR015C)|FD-Score:4.35|P-value:6.86E-6||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MPP6(YNR024W)|FD-Score:-3.16|P-value:7.84E-4||SGD DESC:Nuclear exosome-associated RNA binding protein; involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA); copurifies with ribosomes Gene:MRN1(YPL184C)|FD-Score:3.11|P-value:9.41E-4||SGD DESC:RNA-binding protein that may be involved in translational regulation; binds specific categories of mRNAs, including those that contain upstream open reading frames (uORFs) and internal ribosome entry sites (IRES); interacts genetically with chromatin remodelers and splicing factors, linking chromatin state, splicing and as a result mRNA maturation Gene:MRPS12(YNR036C)|FD-Score:5.86|P-value:2.32E-9||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MRPS17(YMR188C)|FD-Score:3.12|P-value:9.18E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NDL1(YLR254C)|FD-Score:5.31|P-value:5.53E-8||SGD DESC:Homolog of nuclear distribution factor NudE, NUDEL; interacts with Pac1p and regulates dynein targeting to microtubule plus ends Gene:NEW1(YPL226W)|FD-Score:3.96|P-value:3.74E-5||SGD DESC:ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction Gene:OCA5(YHL029C_p)|FD-Score:-3.52|P-value:2.16E-4||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:OPI8(YKR035C_d)|FD-Score:3.1|P-value:9.67E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A Gene:OSH6(YKR003W)|FD-Score:4.55|P-value:2.74E-6||SGD DESC:Member of an oxysterol-binding protein family; overlapping, redundant functions in sterol metabolism and which collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion Gene:PER1(YCR044C)|FD-Score:3.3|P-value:4.80E-4||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PEX7(YDR142C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Peroxisomal signal receptor for the N-terminal nonapeptide signal (PTS2) of peroxisomal matrix proteins; WD repeat protein; defects in human homolog cause lethal rhizomelic chondrodysplasia punctata (RCDP) Gene:PFK2(YMR205C)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PPH21(YDL134C)|FD-Score:3.37|P-value:3.82E-4||SGD DESC:Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph22p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; forms nuclear foci upon DNA replication stress; PPH21 has a paralog, PPH22, that arose from the whole genome duplication Gene:PSY3(YLR376C)|FD-Score:-3.17|P-value:7.68E-4||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; deletion of PSY3 results in a mutator phenotype; deletion increases sensitivity to anticancer drugs oxaliplatin and cisplatin but not mitomycin C Gene:RDH54(YBR073W)|FD-Score:3.31|P-value:4.64E-4||SGD DESC:DNA-dependent ATPase; stimulates strand exchange by modifying the topology of double-stranded DNA; involved in recombinational repair of DNA double-strand breaks during mitosis and meiosis; proposed to be involved in crossover interference; interacts with Dmc1p; stimulates Dmc1p and Rad51p Gene:RIM101(YHL027W)|FD-Score:-3.39|P-value:3.49E-4||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RIM21(YNL294C)|FD-Score:-3.34|P-value:4.22E-4||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RPL27A(YHR010W)|FD-Score:4.55|P-value:2.67E-6||SGD DESC:Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication Gene:RPL31A(YDL075W)|FD-Score:3.46|P-value:2.75E-4||SGD DESC:Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication Gene:RPP1B(YDL130W)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component Gene:RPS30A(YLR287C-A)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication Gene:RTG1(YOL067C)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus Gene:SAS3(YBL052C)|FD-Score:3.91|P-value:4.68E-5||SGD DESC:Histone acetyltransferase catalytic subunit of NuA3 complex that acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal Gene:SDH1(YKL148C)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Flavoprotein subunit of succinate dehydrogenase; couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; FAD binding to Sdh1p is required for the assembly of the succinate dehydrogenase subunits Gene:SEG1(YMR086W)|FD-Score:3.91|P-value:4.61E-5||SGD DESC:Component of eisosome required for proper eisosome assembly; precedes Pil1p/Lsp1p during eisosome formation and controls eisosome length and shape; Seg1p-GFP diffusely distributed and forms heterogeneous patches at the plasma membrane in small buds, also found in medium and large buds; may interact with ribosomes, based on co-purification experiments; expression is repressed by cAMP; similar to A. gossypii SEG gene and to S. pombe Sle1p, important for generating eisosomes Gene:SNF12(YNR023W)|FD-Score:4.02|P-value:2.93E-5||SGD DESC:73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive Gene:SNF4(YGL115W)|FD-Score:6.18|P-value:3.20E-10||SGD DESC:Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress Gene:SOL3(YHR163W)|FD-Score:4.27|P-value:9.68E-6||SGD DESC:6-phosphogluconolactonase; catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p; SOL3 has a paralog, SOL4, that arose from the whole genome duplication Gene:SPT8(YLR055C)|FD-Score:3.22|P-value:6.50E-4||SGD DESC:Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters Gene:SSE1(YPL106C)|FD-Score:5.73|P-value:5.17E-9||SGD DESC:ATPase component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSE1 has a paralog, SSE2, that arose from the whole genome duplication Gene:SWC5(YBR231C)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:TDA6(YPR157W_p)|FD-Score:-4.26|P-value:1.05E-5||SGD DESC:Putative protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; null mutant is sensitive to expression of the top1-T722A allele Gene:TRP1(YDR007W)|FD-Score:3.12|P-value:8.97E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:UPS2(YLR168C)|FD-Score:4.14|P-value:1.71E-5||SGD DESC:Mitochondrial protein involved in phospholipid metabolism; localizes to the intermembrane space; has a role in regulation of phospholipid metabolism by inhibiting conversion of phosphatidylethanolamine to phosphatidylcholine; null mutant has defects in mitochondrial morphology; similar to Ups1p, Ups3p and to human PRELI Gene:VEL1(YGL258W)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has paralog YOR387C Gene:VID27(YNL212W)|FD-Score:4.25|P-value:1.09E-5||SGD DESC:Cytoplasmic protein of unknown function; possibly involved in vacuolar protein degradation; not essential for proteasome-dependent degradation of fructose-1,6-bisphosphatase (FBPase); null mutants exhibit normal growth Gene:YAP3(YHL009C)|FD-Score:3.92|P-value:4.51E-5||SGD DESC:Basic leucine zipper (bZIP) transcription factor Gene:YBL012C(YBL012C_d)|FD-Score:4.55|P-value:2.69E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBL071C(YBL071C_d)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Dubious open reading frame, predicted protein contains a peroxisomal targeting signal Gene:YBR200W-A(YBR200W-A_p)|FD-Score:4.56|P-value:2.53E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YCR090C(YCR090C_p)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YCR090C is not an essential gene Gene:YDL241W(YDL241W_p)|FD-Score:3.21|P-value:6.67E-4||SGD DESC:Putative protein of unknown function; YDL241W is not an essential gene Gene:YER097W(YER097W_d)|FD-Score:5.83|P-value:2.82E-9||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFL054C(YFL054C_p)|FD-Score:-3.23|P-value:6.25E-4||SGD DESC:Putative channel-like protein; similar to Fps1p; mediates passive diffusion of glycerol in the presence of ethanol Gene:YHL044W(YHL044W_p)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; green fluorescent protein (GFP)-fusion protein localizes to the plasma membrane in a punctate pattern Gene:YIR042C(YIR042C_p)|FD-Score:3.14|P-value:8.55E-4||SGD DESC:Putative protein of unknown function; YIR042C is a non-essential gene Gene:YMR187C(YMR187C_p)|FD-Score:4.01|P-value:2.97E-5||SGD DESC:Putative protein of unknown function; YMR187C is not an essential gene Gene:YNR005C(YNR005C_d)|FD-Score:3.97|P-value:3.57E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR048W(YNR048W)|FD-Score:-3.67|P-value:1.22E-4||SGD DESC:Protein that interacts with phospholipid translocase (flippase) Dnf3p; YNR048W has a paralog, CDC50, that arose from the whole genome duplication Gene:YOP1(YPR028W)|FD-Score:5.13|P-value:1.47E-7||SGD DESC:Membrane protein that interacts with Yip1p to mediate membrane traffic; interacts with Sey1p to maintain ER morphology; overexpression leads to cell death and accumulation of internal cell membranes; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; forms ER foci upon DNA replication stress Gene:YOR097C(YOR097C_p)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen; YOR097C is not an essential gene Gene:YPR003C(YPR003C_p)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene Gene:YPR1(YDR368W)|FD-Score:3.1|P-value:9.55E-4||SGD DESC:NADPH-dependent aldo-keto reductase; reduces multiple substrates including 2-methylbutyraldehyde and D,L-glyceraldehyde, expression is induced by osmotic and oxidative stress; functionally redundant with other aldo-keto reductases; protein abundance increases in response to DNA replication stress; YPR1 has a paralog, GCY1, that arose from the whole genome duplication Gene:YUR1(YJL139C)|FD-Score:3.74|P-value:9.29E-5||SGD DESC:Mannosyltransferase involved in protein N-glycosylation; member of the KTR1 family; located in the Golgi apparatus; YUR1 has a paralog, KTR2, that arose from the whole genome duplication Gene:ANT1(YPR128C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation Gene:AYR1(YIL124W)|FD-Score:3.48|P-value:2.46E-4||SGD DESC:NADPH-dependent 1-acyl dihydroxyacetone phosphate reductase found in lipid particles, ER, and mitochondrial outer membrane; involved in phosphatidic acid biosynthesis; required for spore germination; capable of metabolizing steroid hormones Gene:CAM1(YPL048W)|FD-Score:5.12|P-value:1.52E-7||SGD DESC:Nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids; has similarity to translational cofactor EF-1 gamma Gene:CAT5(YOR125C)|FD-Score:-3.96|P-value:3.82E-5||SGD DESC:Protein required for ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes; required for gluconeogenic gene activation Gene:CBR1(YIL043C)|FD-Score:3.82|P-value:6.65E-5||SGD DESC:Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia Gene:CCC2(YDR270W)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism Gene:COQ10(YOL008W)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q<sub>6</sub> to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes Gene:CTF3(YLR381W)|FD-Score:3.84|P-value:6.20E-5||SGD DESC:Outer kinetochore protein that forms a complex with Mcm16p and Mcm22p; may bind the kinetochore to spindle microtubules; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-I and fission yeast mis6 Gene:ECM38(YLR299W)|FD-Score:3.3|P-value:4.88E-4||SGD DESC:Gamma-glutamyltranspeptidase, major glutathione-degrading enzyme; involved in detoxification of electrophilic xenobiotics; expression induced mainly by nitrogen starvation Gene:EMC4(YGL231C)|FD-Score:-4.11|P-value:1.96E-5||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4, fly CG11137, human TMM85 Gene:EPS1(YIL005W)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:ER protein with chaperone and co-chaperone activity, involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family Gene:ERD1(YDR414C)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p) Gene:ERG4(YGL012W)|FD-Score:4.09|P-value:2.16E-5||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FIR1(YER032W)|FD-Score:3.53|P-value:2.12E-4||SGD DESC:Protein involved in 3' mRNA processing, interacts with Ref2p; potential Cdc28p substrate Gene:KAP104(YBR017C)|FD-Score:3.78|P-value:7.85E-5||SGD DESC:Transportin or cytosolic karyopherin beta 2; functions in the rg-nuclear localization signal-mediated nuclear import/reimport of mRNA-binding proteins Nab2p and Hrp1p; regulates asymmetric protein synthesis in daughter cells during mitosis Gene:LCB4(YOR171C)|FD-Score:3.77|P-value:8.30E-5||SGD DESC:Sphingoid long-chain base kinase, responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes Gene:MAG2(YLR427W)|FD-Score:-3.53|P-value:2.08E-4||SGD DESC:Cytoplasmic protein of unknown function; induced in response to mycotoxin patulin; ubiquitinated protein similar to the human ring finger motif protein RNF10; predicted to be involved in repair of alkylated DNA due to interaction with MAG1 Gene:MDH2(YOL126C)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Cytoplasmic malate dehydrogenase, one of three isozymes that catalyze interconversion of malate and oxaloacetate; involved in the glyoxylate cycle and gluconeogenesis during growth on two-carbon compounds; interacts with Pck1p and Fbp1 Gene:MNN2(YBR015C)|FD-Score:4.35|P-value:6.86E-6||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MPP6(YNR024W)|FD-Score:-3.16|P-value:7.84E-4||SGD DESC:Nuclear exosome-associated RNA binding protein; involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA); copurifies with ribosomes Gene:MRN1(YPL184C)|FD-Score:3.11|P-value:9.41E-4||SGD DESC:RNA-binding protein that may be involved in translational regulation; binds specific categories of mRNAs, including those that contain upstream open reading frames (uORFs) and internal ribosome entry sites (IRES); interacts genetically with chromatin remodelers and splicing factors, linking chromatin state, splicing and as a result mRNA maturation Gene:MRPS12(YNR036C)|FD-Score:5.86|P-value:2.32E-9||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MRPS17(YMR188C)|FD-Score:3.12|P-value:9.18E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NDL1(YLR254C)|FD-Score:5.31|P-value:5.53E-8||SGD DESC:Homolog of nuclear distribution factor NudE, NUDEL; interacts with Pac1p and regulates dynein targeting to microtubule plus ends Gene:NEW1(YPL226W)|FD-Score:3.96|P-value:3.74E-5||SGD DESC:ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction Gene:OCA5(YHL029C_p)|FD-Score:-3.52|P-value:2.16E-4||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:OPI8(YKR035C_d)|FD-Score:3.1|P-value:9.67E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A Gene:OSH6(YKR003W)|FD-Score:4.55|P-value:2.74E-6||SGD DESC:Member of an oxysterol-binding protein family; overlapping, redundant functions in sterol metabolism and which collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion Gene:PER1(YCR044C)|FD-Score:3.3|P-value:4.80E-4||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PEX7(YDR142C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Peroxisomal signal receptor for the N-terminal nonapeptide signal (PTS2) of peroxisomal matrix proteins; WD repeat protein; defects in human homolog cause lethal rhizomelic chondrodysplasia punctata (RCDP) Gene:PFK2(YMR205C)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PPH21(YDL134C)|FD-Score:3.37|P-value:3.82E-4||SGD DESC:Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph22p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; forms nuclear foci upon DNA replication stress; PPH21 has a paralog, PPH22, that arose from the whole genome duplication Gene:PSY3(YLR376C)|FD-Score:-3.17|P-value:7.68E-4||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; deletion of PSY3 results in a mutator phenotype; deletion increases sensitivity to anticancer drugs oxaliplatin and cisplatin but not mitomycin C Gene:RDH54(YBR073W)|FD-Score:3.31|P-value:4.64E-4||SGD DESC:DNA-dependent ATPase; stimulates strand exchange by modifying the topology of double-stranded DNA; involved in recombinational repair of DNA double-strand breaks during mitosis and meiosis; proposed to be involved in crossover interference; interacts with Dmc1p; stimulates Dmc1p and Rad51p Gene:RIM101(YHL027W)|FD-Score:-3.39|P-value:3.49E-4||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RIM21(YNL294C)|FD-Score:-3.34|P-value:4.22E-4||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RPL27A(YHR010W)|FD-Score:4.55|P-value:2.67E-6||SGD DESC:Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication Gene:RPL31A(YDL075W)|FD-Score:3.46|P-value:2.75E-4||SGD DESC:Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication Gene:RPP1B(YDL130W)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component Gene:RPS30A(YLR287C-A)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication Gene:RTG1(YOL067C)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus Gene:SAS3(YBL052C)|FD-Score:3.91|P-value:4.68E-5||SGD DESC:Histone acetyltransferase catalytic subunit of NuA3 complex that acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal Gene:SDH1(YKL148C)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Flavoprotein subunit of succinate dehydrogenase; couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; FAD binding to Sdh1p is required for the assembly of the succinate dehydrogenase subunits Gene:SEG1(YMR086W)|FD-Score:3.91|P-value:4.61E-5||SGD DESC:Component of eisosome required for proper eisosome assembly; precedes Pil1p/Lsp1p during eisosome formation and controls eisosome length and shape; Seg1p-GFP diffusely distributed and forms heterogeneous patches at the plasma membrane in small buds, also found in medium and large buds; may interact with ribosomes, based on co-purification experiments; expression is repressed by cAMP; similar to A. gossypii SEG gene and to S. pombe Sle1p, important for generating eisosomes Gene:SNF12(YNR023W)|FD-Score:4.02|P-value:2.93E-5||SGD DESC:73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive Gene:SNF4(YGL115W)|FD-Score:6.18|P-value:3.20E-10||SGD DESC:Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress Gene:SOL3(YHR163W)|FD-Score:4.27|P-value:9.68E-6||SGD DESC:6-phosphogluconolactonase; catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p; SOL3 has a paralog, SOL4, that arose from the whole genome duplication Gene:SPT8(YLR055C)|FD-Score:3.22|P-value:6.50E-4||SGD DESC:Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters Gene:SSE1(YPL106C)|FD-Score:5.73|P-value:5.17E-9||SGD DESC:ATPase component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSE1 has a paralog, SSE2, that arose from the whole genome duplication Gene:SWC5(YBR231C)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:TDA6(YPR157W_p)|FD-Score:-4.26|P-value:1.05E-5||SGD DESC:Putative protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; null mutant is sensitive to expression of the top1-T722A allele Gene:TRP1(YDR007W)|FD-Score:3.12|P-value:8.97E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:UPS2(YLR168C)|FD-Score:4.14|P-value:1.71E-5||SGD DESC:Mitochondrial protein involved in phospholipid metabolism; localizes to the intermembrane space; has a role in regulation of phospholipid metabolism by inhibiting conversion of phosphatidylethanolamine to phosphatidylcholine; null mutant has defects in mitochondrial morphology; similar to Ups1p, Ups3p and to human PRELI Gene:VEL1(YGL258W)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has paralog YOR387C Gene:VID27(YNL212W)|FD-Score:4.25|P-value:1.09E-5||SGD DESC:Cytoplasmic protein of unknown function; possibly involved in vacuolar protein degradation; not essential for proteasome-dependent degradation of fructose-1,6-bisphosphatase (FBPase); null mutants exhibit normal growth Gene:YAP3(YHL009C)|FD-Score:3.92|P-value:4.51E-5||SGD DESC:Basic leucine zipper (bZIP) transcription factor Gene:YBL012C(YBL012C_d)|FD-Score:4.55|P-value:2.69E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBL071C(YBL071C_d)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Dubious open reading frame, predicted protein contains a peroxisomal targeting signal Gene:YBR200W-A(YBR200W-A_p)|FD-Score:4.56|P-value:2.53E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YCR090C(YCR090C_p)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YCR090C is not an essential gene Gene:YDL241W(YDL241W_p)|FD-Score:3.21|P-value:6.67E-4||SGD DESC:Putative protein of unknown function; YDL241W is not an essential gene Gene:YER097W(YER097W_d)|FD-Score:5.83|P-value:2.82E-9||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFL054C(YFL054C_p)|FD-Score:-3.23|P-value:6.25E-4||SGD DESC:Putative channel-like protein; similar to Fps1p; mediates passive diffusion of glycerol in the presence of ethanol Gene:YHL044W(YHL044W_p)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; green fluorescent protein (GFP)-fusion protein localizes to the plasma membrane in a punctate pattern Gene:YIR042C(YIR042C_p)|FD-Score:3.14|P-value:8.55E-4||SGD DESC:Putative protein of unknown function; YIR042C is a non-essential gene Gene:YMR187C(YMR187C_p)|FD-Score:4.01|P-value:2.97E-5||SGD DESC:Putative protein of unknown function; YMR187C is not an essential gene Gene:YNR005C(YNR005C_d)|FD-Score:3.97|P-value:3.57E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR048W(YNR048W)|FD-Score:-3.67|P-value:1.22E-4||SGD DESC:Protein that interacts with phospholipid translocase (flippase) Dnf3p; YNR048W has a paralog, CDC50, that arose from the whole genome duplication Gene:YOP1(YPR028W)|FD-Score:5.13|P-value:1.47E-7||SGD DESC:Membrane protein that interacts with Yip1p to mediate membrane traffic; interacts with Sey1p to maintain ER morphology; overexpression leads to cell death and accumulation of internal cell membranes; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; forms ER foci upon DNA replication stress Gene:YOR097C(YOR097C_p)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen; YOR097C is not an essential gene Gene:YPR003C(YPR003C_p)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene Gene:YPR1(YDR368W)|FD-Score:3.1|P-value:9.55E-4||SGD DESC:NADPH-dependent aldo-keto reductase; reduces multiple substrates including 2-methylbutyraldehyde and D,L-glyceraldehyde, expression is induced by osmotic and oxidative stress; functionally redundant with other aldo-keto reductases; protein abundance increases in response to DNA replication stress; YPR1 has a paralog, GCY1, that arose from the whole genome duplication Gene:YUR1(YJL139C)|FD-Score:3.74|P-value:9.29E-5||SGD DESC:Mannosyltransferase involved in protein N-glycosylation; member of the KTR1 family; located in the Golgi apparatus; YUR1 has a paralog, KTR2, that arose from the whole genome duplication

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR146W_d4.483.77E-60.41YOR146W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C
YGR013W4.464.07E-60.41SNU71Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart
YNL308C4.231.14E-50.41KRI1Essential nucleolar protein required for 40S ribosome biogenesis; associate with snR30; physically and functionally interacts with Krr1p
YNL110C4.221.23E-50.41NOP15Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm
YBR190W_d4.191.41E-50.41YBR190W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W
YLR197W3.778.03E-50.04NOP56Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects
YIL046W3.739.55E-50.11MET30F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus
YNL112W3.621.45E-47.86E-4DBP2ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites
YDR328C3.621.45E-40.02SKP1Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress
YGL142C3.611.55E-40.01GPI10Integral membrane protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; putative alpha 1,2 mannosyltransferase required for addition of the third mannose onto the GPI core structure; human PIG-Bp is a functional homolog
YCR012W3.601.58E-40.22PGK13-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phosphate of 1,3-bisphosphoglycerate to ADP to produce ATP; key enzyme in glycolysis and gluconeogenesis
YHR088W3.383.57E-40.10RPF1Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA
YIL061C3.285.14E-40.08SNP1Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress
YJL087C3.206.78E-40.23TRL1tRNA ligase, required for tRNA splicing and for both splicing and translation of HAC1 mRNA in the UPR; has phosphodiesterase, polynucleotide kinase, and ligase activities; localized at the inner nuclear envelope and partially to polysomes
YDR060W2.980.001460.08MAK21Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL115W6.183.20E-10SNF4Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress
YNR036C5.862.32E-9MRPS12Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins
YER097W_d5.832.82E-9YER097W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YPL106C5.735.17E-9SSE1ATPase component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSE1 has a paralog, SSE2, that arose from the whole genome duplication
YLR254C5.315.53E-8NDL1Homolog of nuclear distribution factor NudE, NUDEL; interacts with Pac1p and regulates dynein targeting to microtubule plus ends
YPR028W5.131.47E-7YOP1Membrane protein that interacts with Yip1p to mediate membrane traffic; interacts with Sey1p to maintain ER morphology; overexpression leads to cell death and accumulation of internal cell membranes; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; forms ER foci upon DNA replication stress
YPL048W5.121.52E-7CAM1Nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids; has similarity to translational cofactor EF-1 gamma
YBR200W-A_p4.562.53E-6YBR200W-A_pPutative protein of unknown function; identified by fungal homology and RT-PCR
YHR010W4.552.67E-6RPL27ARibosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication
YBL012C_d4.552.69E-6YBL012C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YKR003W4.552.74E-6OSH6Member of an oxysterol-binding protein family; overlapping, redundant functions in sterol metabolism and which collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion
YBR015C4.356.86E-6MNN2Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment
YHR163W4.279.68E-6SOL36-phosphogluconolactonase; catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p; SOL3 has a paralog, SOL4, that arose from the whole genome duplication
YNL212W4.251.09E-5VID27Cytoplasmic protein of unknown function; possibly involved in vacuolar protein degradation; not essential for proteasome-dependent degradation of fructose-1,6-bisphosphatase (FBPase); null mutants exhibit normal growth
YLR287C-A4.171.54E-5RPS30AProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication

GO enrichment analysis for SGTC_1032
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0765.41E-9SGTC_24977-hydroxyflavone 100.0 μMMicrosource (Natural product library)52818940.102941
0.0656.58E-7SGTC_1027k015-0025 8.7 μMChemDiv (Drug-like library)35989550.0897436
0.0647.15E-7SGTC_797k781-0471 270.0 μMChemDiv (Drug-like library)237381290.121622RPP1 & pyrimidine depletion
0.0621.96E-6SGTC_31799107801 49.5 μMChembridge (Drug-like library)48182590.208333
0.0612.58E-6SGTC_1038k292-0204 154.0 μMChemDiv (Drug-like library)44542120.0945946
0.0595.91E-6SGTC_13772944-0135 198.0 μMChemDiv (Drug-like library)18941370.0963855calcium & mitochondrial duress
0.0588.45E-6SGTC_28097964133 13.0 μMChembridge (Drug-like library)12536650.17948760S ribosome export
0.0571.08E-5SGTC_10410206-0046 107.0 μMChemDiv (Drug-like library)504940.0972222
0.0552.19E-5SGTC_1080509-0257 621.1 μMChemDiv (Drug-like library)5366240.15873
0.0552.40E-5SGTC_1037k284-1091 78.7 μMChemDiv (Drug-like library)33347110.0958904
0.0542.80E-5SGTC_1839st053978 48.0 μMTimTec (Natural product derivative library)37883980.127907
0.0542.92E-5SGTC_120rhodamine 6G 52.3 μMChemDiv (Drug-like library)652110.195402mitochondrial processes
0.0535.35E-5SGTC_204PMA1.184 62.4 μMChemDiv (Drug-like library)22522130.151899TRP & mitochondrial translation
0.0526.06E-5SGTC_7353076-0345 636.0 μMChemDiv (Drug-like library)28889900.0909091
0.0527.26E-5SGTC_7700389-1121 67.2 μMChemDiv (Drug-like library)67531080.111111RPP1 & pyrimidine depletion

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_831k061-1017217 μM0.7916671240153ChemDiv (Drug-like library)512.553125.3636translation
SGTC_2798781363745.45 μM0.557692956368Chembridge (Drug-like library)277.2660263.34714
SGTC_3207911416349.47 μM0.421053849233Chembridge (Drug-like library)269.338263.43812
SGTC_2826799254119.48 μM0.42985066Chembridge (Drug-like library)297.6845063.52514
SGTC_3184910875849.47 μM0.4902885Chembridge (Drug-like library)241.28512.94712
SGTC_2912796429810.93 μM0.3965522975850Chembridge (Drug-like library)299.364243.60823cell wall
SGTC_3206911435049.47 μM0.38596517087243Chembridge (Drug-like library)283.321783.53513
SGTC_2988907364071.43 μM0.3620699102229Chembridge (Drug-like library)283.364843.91512
SGTC_1300136-0219337.66 μM0.357143234244ChemDiv (Drug-like library)296.148643.77812
SGTC_7950685-026578 μM0.357143302754ChemDiv (Drug-like library)340.599643.86212excess fatty acid