k072-0165

N-[(Z)-(4-bromothiophen-2-yl)methylideneamino]-3,5-dichloropyridin-4-amine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1034
Screen concentration 12.7 μM
Source ChemDiv (Drug-like library)
PubChem CID 5835461
SMILES C1=C(SC=C1Br)C=NNC2=C(C=NC=C2Cl)Cl
Standardized SMILES Clc1cncc(Cl)c1NN=Cc2cc(Br)cs2
Molecular weight 351.0497
ALogP 4.33
H-bond donor count 1
H-bond acceptor count 4
Response signature Golgi

Pool Growth Kinetics
% growth inhibition (Het. pool) 12.55
% growth inhibition (Hom. pool) 8.01


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5835461
Download HIP data (tab-delimited text)  (excel)
Gene:CDC11(YJR076C)|FD-Score:3.26|P-value:5.52E-4|Clearance:0.01||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CDC19(YAL038W)|FD-Score:3.44|P-value:2.95E-4|Clearance:0.15||SGD DESC:Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication Gene:CDC20(YGL116W)|FD-Score:4.48|P-value:3.75E-6|Clearance:0.26||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC43(YGL155W)|FD-Score:3.22|P-value:6.42E-4|Clearance:0.01||SGD DESC:Beta subunit of geranylgeranyltransferase type I, catalyzes geranylgeranylation to the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis Gene:CTR86(YCR054C)|FD-Score:3.87|P-value:5.38E-5|Clearance:0.34||SGD DESC:Essential protein of unknown function; with orthologs in Ashbya gossypii and Candida albicans; similar to human ATXN10, mutations in which cause spinocerebellar ataxia type 10; codon usage corresponds to that observed for yeast genes expressed at low levels; relative distribution to the nucleus increases upon DNA replication stress Gene:PRI2(YKL045W)|FD-Score:-3.26|P-value:5.64E-4|Clearance:0||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:PRO3(YER023W)|FD-Score:3.25|P-value:5.76E-4|Clearance:0.03||SGD DESC:Delta 1-pyrroline-5-carboxylate reductase, catalyzes the last step in proline biosynthesis Gene:RPL18A(YOL120C)|FD-Score:3.28|P-value:5.16E-4|Clearance:0.02||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RRN3(YKL125W)|FD-Score:-3.91|P-value:4.57E-5|Clearance:0||SGD DESC:Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo Gene:RVB2(YPL235W)|FD-Score:8.03|P-value:4.69E-16|Clearance:1.92||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SEC13(YLR208W)|FD-Score:4.73|P-value:1.14E-6|Clearance:0.25||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SNU71(YGR013W)|FD-Score:6.12|P-value:4.72E-10|Clearance:1.39||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart Gene:SSS1(YDR086C)|FD-Score:3.53|P-value:2.07E-4|Clearance:0.06||SGD DESC:Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p Gene:TRL1(YJL087C)|FD-Score:3.47|P-value:2.57E-4|Clearance:0.04||SGD DESC:tRNA ligase, required for tRNA splicing and for both splicing and translation of HAC1 mRNA in the UPR; has phosphodiesterase, polynucleotide kinase, and ligase activities; localized at the inner nuclear envelope and partially to polysomes Gene:TRM5(YHR070W)|FD-Score:4.21|P-value:1.25E-5|Clearance:0.34||SGD DESC:tRNA(m(1)G37)methyltransferase, methylates a tRNA base adjacent to the anticodon that has a role in prevention of frameshifting; highly conserved across Archaea, Bacteria, and Eukarya Gene:YNL150W(YNL150W_d)|FD-Score:-4|P-value:3.13E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking Gene:YSH1(YLR277C)|FD-Score:3.21|P-value:6.68E-4|Clearance:0.2||SGD DESC:Putative endoribonuclease; subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs; protein abundance increases in response to DNA replication stress; YSH1 has a paralog, SYC1, that arose from the whole genome duplication Gene:CDC11(YJR076C)|FD-Score:3.26|P-value:5.52E-4|Clearance:0.01||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CDC19(YAL038W)|FD-Score:3.44|P-value:2.95E-4|Clearance:0.15||SGD DESC:Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication Gene:CDC20(YGL116W)|FD-Score:4.48|P-value:3.75E-6|Clearance:0.26||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC43(YGL155W)|FD-Score:3.22|P-value:6.42E-4|Clearance:0.01||SGD DESC:Beta subunit of geranylgeranyltransferase type I, catalyzes geranylgeranylation to the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis Gene:CTR86(YCR054C)|FD-Score:3.87|P-value:5.38E-5|Clearance:0.34||SGD DESC:Essential protein of unknown function; with orthologs in Ashbya gossypii and Candida albicans; similar to human ATXN10, mutations in which cause spinocerebellar ataxia type 10; codon usage corresponds to that observed for yeast genes expressed at low levels; relative distribution to the nucleus increases upon DNA replication stress Gene:PRI2(YKL045W)|FD-Score:-3.26|P-value:5.64E-4|Clearance:0||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:PRO3(YER023W)|FD-Score:3.25|P-value:5.76E-4|Clearance:0.03||SGD DESC:Delta 1-pyrroline-5-carboxylate reductase, catalyzes the last step in proline biosynthesis Gene:RPL18A(YOL120C)|FD-Score:3.28|P-value:5.16E-4|Clearance:0.02||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RRN3(YKL125W)|FD-Score:-3.91|P-value:4.57E-5|Clearance:0||SGD DESC:Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo Gene:RVB2(YPL235W)|FD-Score:8.03|P-value:4.69E-16|Clearance:1.92||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SEC13(YLR208W)|FD-Score:4.73|P-value:1.14E-6|Clearance:0.25||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SNU71(YGR013W)|FD-Score:6.12|P-value:4.72E-10|Clearance:1.39||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart Gene:SSS1(YDR086C)|FD-Score:3.53|P-value:2.07E-4|Clearance:0.06||SGD DESC:Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p Gene:TRL1(YJL087C)|FD-Score:3.47|P-value:2.57E-4|Clearance:0.04||SGD DESC:tRNA ligase, required for tRNA splicing and for both splicing and translation of HAC1 mRNA in the UPR; has phosphodiesterase, polynucleotide kinase, and ligase activities; localized at the inner nuclear envelope and partially to polysomes Gene:TRM5(YHR070W)|FD-Score:4.21|P-value:1.25E-5|Clearance:0.34||SGD DESC:tRNA(m(1)G37)methyltransferase, methylates a tRNA base adjacent to the anticodon that has a role in prevention of frameshifting; highly conserved across Archaea, Bacteria, and Eukarya Gene:YNL150W(YNL150W_d)|FD-Score:-4|P-value:3.13E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking Gene:YSH1(YLR277C)|FD-Score:3.21|P-value:6.68E-4|Clearance:0.2||SGD DESC:Putative endoribonuclease; subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs; protein abundance increases in response to DNA replication stress; YSH1 has a paralog, SYC1, that arose from the whole genome duplication

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5835461
Download HOP data (tab-delimited text)  (excel)
Gene:AFT2(YPL202C)|FD-Score:-3.15|P-value:8.15E-4||SGD DESC:Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication Gene:AGX1(YFL030W)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Alanine:glyoxylate aminotransferase (AGT), catalyzes the synthesis of glycine from glyoxylate, which is one of three pathways for glycine biosynthesis in yeast; has similarity to mammalian and plant alanine:glyoxylate aminotransferases Gene:AHA1(YDR214W)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Co-chaperone that binds to Hsp82p and activates its ATPase activity; similar to Hch1p; expression is regulated by stresses such as heat shock; protein abundance increases in response to DNA replication stress Gene:AHC2(YCR082W)|FD-Score:-3.09|P-value:9.97E-4||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:APS2(YJR058C)|FD-Score:4.3|P-value:8.41E-6||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-2, which is involved in protein sorting at the plasma membrane; related to the sigma subunit of the mammalian plasma membrane clathrin-associated protein (AP-2) complex Gene:ATP19(YOL077W-A)|FD-Score:-3.63|P-value:1.42E-4||SGD DESC:Subunit k of the mitochondrial F1F0 ATP synthase, which is a large enzyme complex required for ATP synthesis; associated only with the dimeric form of ATP synthase Gene:CLC1(YGR167W)|FD-Score:5.52|P-value:1.67E-8||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:COG5(YNL051W)|FD-Score:8.32|P-value:4.49E-17||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG6(YNL041C)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:7.29|P-value:1.55E-13||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COS10(YNR075W)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:COS6(YGR295C)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:COX5A(YNL052W)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Subunit Va of cytochrome c oxidase; cytochrome c oxidase is the terminal member of the mitochondrial inner membrane electron transport chain; Cox5Ap is predominantly expressed during aerobic growth while its isoform Vb (Cox5Bp) is expressed during anaerobic growth; COX5A has a paralog, COX5B, that arose from the whole genome duplication Gene:DAL81(YIR023W)|FD-Score:3.1|P-value:9.69E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DIA3(YDL024C)|FD-Score:3.29|P-value:5.00E-4||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth Gene:ECM23(YPL021W)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:Non-essential protein of unconfirmed function; affects pre-rRNA processing, may act as a negative regulator of the transcription of genes involved in pseudohyphal growth; homologous to Srd1p Gene:EMP70(YLR083C)|FD-Score:-4.55|P-value:2.75E-6||SGD DESC:Protein with a role in cellular adhesion, filamentous growth, and endosome-to-vacuole sorting; similar to Tmn2p and Tmn3p; member of Transmembrane Nine family of proteins with 9 transmembrane segments Gene:GCN20(YFR009W)|FD-Score:-4.95|P-value:3.62E-7||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GEF1(YJR040W)|FD-Score:3.87|P-value:5.49E-5||SGD DESC:Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism Gene:GIR2(YDR152W)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Highly-acidic cytoplasmic RWD domain-containing protein of unknown function; forms a complex with Rbg2p; interacts with Rbg1p and Gcn1p; associates with translating ribosomes; putative intrinsically unstructured protein Gene:GPR1(YDL035C)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis Gene:HAL9(YOL089C)|FD-Score:3.21|P-value:6.71E-4||SGD DESC:Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication Gene:HNM1(YGL077C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Choline/ethanolamine transporter; involved in the uptake of nitrogen mustard and the uptake of glycine betaine during hypersaline stress; co-regulated with phospholipid biosynthetic genes and negatively regulated by choline and myo-inositol Gene:HOF1(YMR032W)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:Bud neck-localized, SH3 domain-containing protein required for cytokinesis; is phosphorylated by Dbf2p; regulates actomyosin ring dynamics and septin localization; interacts with the formins, Bni1p and Bnr1p, and with Cyk3p, Vrp1p, and Bni5p Gene:IRS4(YKR019C)|FD-Score:3.77|P-value:8.31E-5||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KTR7(YIL085C)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR7 has a paralog, KTR5, that arose from the whole genome duplication Gene:MEC3(YLR288C)|FD-Score:-3.64|P-value:1.35E-4||SGD DESC:DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1 Gene:MPC2(YHR162W)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium Gene:OSH3(YHR073W)|FD-Score:3.13|P-value:8.74E-4||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PAM1(YDR251W)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Essential protein of unknown function; exhibits variable expression during colony morphogenesis; overexpression permits survival without protein phosphatase 2A, inhibits growth, and induces a filamentous phenotype Gene:PPA2(YMR267W)|FD-Score:3.21|P-value:6.71E-4||SGD DESC:Mitochondrial inorganic pyrophosphatase, required for mitochondrial function and possibly involved in energy generation from inorganic pyrophosphate Gene:PPM1(YDR435C)|FD-Score:3.21|P-value:6.75E-4||SGD DESC:Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits Gene:PRM2(YIL037C)|FD-Score:4.21|P-value:1.29E-5||SGD DESC:Pheromone-regulated protein, predicted to have 4 transmembrane segments and a coiled coil domain; regulated by Ste12p; required for efficient nuclear fusion Gene:PUT2(YHR037W)|FD-Score:-6.25|P-value:2.09E-10||SGD DESC:Delta-1-pyrroline-5-carboxylate dehydrogenase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of the human homolog causes HPII, an autosomal recessive inborn error of metabolism Gene:REV1(YOR346W)|FD-Score:-3.15|P-value:8.25E-4||SGD DESC:Deoxycytidyl transferase; involved in repair of abasic sites and adducted guanines in damaged DNA by translesion synthesis (TLS); forms a complex with the subunits of DNA polymerase zeta, Rev3p and Rev7p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RIM8(YGL045W)|FD-Score:5.11|P-value:1.64E-7||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:RNH201(YNL072W)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:Ribonuclease H2 catalytic subunit, removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome Gene:RPL24B(YGR148C)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication Gene:RSM24(YDR175C)|FD-Score:-3.25|P-value:5.74E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RTC2(YBR147W)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; positive regulation by Lys14p suggests that lysine may be the primary substrate; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter Gene:RTT103(YDR289C)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition Gene:SEC66(YBR171W)|FD-Score:4.79|P-value:8.18E-7||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SHE4(YOR035C)|FD-Score:-3.51|P-value:2.28E-4||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SLT2(YHR030C)|FD-Score:4.29|P-value:9.08E-6||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNF12(YNR023W)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive Gene:SNF7(YLR025W)|FD-Score:-3.92|P-value:4.46E-5||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SOV1(YMR066W)|FD-Score:10.1|P-value:2.89E-24||SGD DESC:Mitochondrial protein of unknown function Gene:SPF1(YEL031W)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SPT7(YBR081C)|FD-Score:3.77|P-value:8.22E-5||SGD DESC:Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex Gene:SSF2(YDR312W)|FD-Score:4.51|P-value:3.29E-6||SGD DESC:Protein required for ribosomal large subunit maturation; functionally redundant with Ssf1p; member of the Brix family; SSF2 has a paralog, SSF1, that arose from the whole genome duplication Gene:SUR4(YLR372W)|FD-Score:3.23|P-value:6.09E-4||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SVF1(YDR346C)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Protein with a potential role in cell survival pathways, required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance Gene:SYF2(YGR129W)|FD-Score:-4.95|P-value:3.63E-7||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:TAT1(YBR069C)|FD-Score:7.13|P-value:5.13E-13||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TFB5(YDR079C-A)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Component of the RNA polymerase II general transcription and DNA repair factor TFIIH; involved in transcription initiation and in nucleotide-excision repair; homolog of Chlamydomonas reinhardtii REX1-S protein involved in DNA repair Gene:THI12(YNL332W)|FD-Score:4.06|P-value:2.40E-5||SGD DESC:Protein involved in synthesis of the thiamine precursor hydroxymethylpyrimidine (HMP); member of a subtelomeric gene family including THI5, THI11, THI12, and THI13 Gene:UBC5(YDR059C)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived, abnormal, or excess proteins, including histone H3; central component of the cellular stress response; expression is heat inducible; protein abundance increases in response to DNA replication stress; UBC5 has a paralog, UBC4, that arose from the whole genome duplication Gene:UIP4(YPL186C)|FD-Score:5.11|P-value:1.63E-7||SGD DESC:Protein that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates; detected in a phosphorylated state in the mitochondrial outer membrane; also detected in ER and nuclear envelope Gene:URA7(YBL039C)|FD-Score:-3.34|P-value:4.24E-4||SGD DESC:Major CTP synthase isozyme (see also URA8); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA7 has a paralog, URA8, that arose from the whole genome duplication Gene:URM1(YIL008W)|FD-Score:3.86|P-value:5.69E-5||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:VAM3(YOR106W)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region Gene:VAM6(YDL077C)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p Gene:VBA2(YBR293W)|FD-Score:4.97|P-value:3.29E-7||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VMA9(YCL005W-A)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis Gene:VPS36(YLR417W)|FD-Score:3.72|P-value:9.78E-5||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:VPS61(YDR136C_d)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YBR016W(YBR016W)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Tail-anchored plasma membrane protein with a conserved CYSTM module; predicted to be palmitoylated; has similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions; YBR016W has a paralog, YDL012C, that arose from the whole genome duplication Gene:YCL012C(YCL012C)|FD-Score:3.14|P-value:8.48E-4||SGD DESC:Putative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene Gene:YCR025C(YCR025C_d)|FD-Score:10.6|P-value:9.96E-27||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR025C is not an essential gene Gene:YDR230W(YDR230W_d)|FD-Score:3.14|P-value:8.42E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20 Gene:YIL032C(YIL032C_d)|FD-Score:-3.37|P-value:3.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIR044C(YIR044C)|FD-Score:-4.83|P-value:6.99E-7||SGD DESC:Possible pseudogene in strain S288C; YIR044C and the adjacent ORF, YIR043C, together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family Gene:YKR051W(YKR051W_p)|FD-Score:3.26|P-value:5.67E-4||SGD DESC:Putative protein of unknown function Gene:YLR112W(YLR112W_d)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML131W(YML131W_p)|FD-Score:3.1|P-value:9.74E-4||SGD DESC:Protein of unknown function; similar to medium chain dehydrogenase/reductases; expression induced by stresses including osmotic shock, DNA damaging agents, and other chemicals; GFP-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YMR265C(YMR265C_p)|FD-Score:4.43|P-value:4.64E-6||SGD DESC:Putative protein of unknown function Gene:YNL108C(YNL108C_p)|FD-Score:4.28|P-value:9.34E-6||SGD DESC:Putative protein of unknown function; similar to prokaryotic phosphotransfer enzymes; null mutant shows alterations in glucose metabolism; GFP-fusion protein localizes to the cytoplasm and nucleus; YNL108C has a paralog, TFC7, that arose from the whole genome duplication Gene:YNL198C(YNL198C_d)|FD-Score:-4.11|P-value:2.00E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOX1(YML027W)|FD-Score:3.2|P-value:6.93E-4||SGD DESC:Homeobox transcriptional repressor; binds to Mcm1p and to early cell cycle boxes (ECBs) in the promoters of cell cycle-regulated genes expressed in M/G1 phase; expression is cell cycle-regulated; potential Cdc28p substrate; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOX1 has a paralog, YHP1, that arose from the whole genome duplication Gene:YPR084W(YPR084W_p)|FD-Score:3.91|P-value:4.67E-5||SGD DESC:Putative protein of unknown function Gene:AFT2(YPL202C)|FD-Score:-3.15|P-value:8.15E-4||SGD DESC:Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication Gene:AGX1(YFL030W)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Alanine:glyoxylate aminotransferase (AGT), catalyzes the synthesis of glycine from glyoxylate, which is one of three pathways for glycine biosynthesis in yeast; has similarity to mammalian and plant alanine:glyoxylate aminotransferases Gene:AHA1(YDR214W)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Co-chaperone that binds to Hsp82p and activates its ATPase activity; similar to Hch1p; expression is regulated by stresses such as heat shock; protein abundance increases in response to DNA replication stress Gene:AHC2(YCR082W)|FD-Score:-3.09|P-value:9.97E-4||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:APS2(YJR058C)|FD-Score:4.3|P-value:8.41E-6||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-2, which is involved in protein sorting at the plasma membrane; related to the sigma subunit of the mammalian plasma membrane clathrin-associated protein (AP-2) complex Gene:ATP19(YOL077W-A)|FD-Score:-3.63|P-value:1.42E-4||SGD DESC:Subunit k of the mitochondrial F1F0 ATP synthase, which is a large enzyme complex required for ATP synthesis; associated only with the dimeric form of ATP synthase Gene:CLC1(YGR167W)|FD-Score:5.52|P-value:1.67E-8||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:COG5(YNL051W)|FD-Score:8.32|P-value:4.49E-17||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG6(YNL041C)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:7.29|P-value:1.55E-13||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COS10(YNR075W)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:COS6(YGR295C)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:COX5A(YNL052W)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Subunit Va of cytochrome c oxidase; cytochrome c oxidase is the terminal member of the mitochondrial inner membrane electron transport chain; Cox5Ap is predominantly expressed during aerobic growth while its isoform Vb (Cox5Bp) is expressed during anaerobic growth; COX5A has a paralog, COX5B, that arose from the whole genome duplication Gene:DAL81(YIR023W)|FD-Score:3.1|P-value:9.69E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DIA3(YDL024C)|FD-Score:3.29|P-value:5.00E-4||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth Gene:ECM23(YPL021W)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:Non-essential protein of unconfirmed function; affects pre-rRNA processing, may act as a negative regulator of the transcription of genes involved in pseudohyphal growth; homologous to Srd1p Gene:EMP70(YLR083C)|FD-Score:-4.55|P-value:2.75E-6||SGD DESC:Protein with a role in cellular adhesion, filamentous growth, and endosome-to-vacuole sorting; similar to Tmn2p and Tmn3p; member of Transmembrane Nine family of proteins with 9 transmembrane segments Gene:GCN20(YFR009W)|FD-Score:-4.95|P-value:3.62E-7||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GEF1(YJR040W)|FD-Score:3.87|P-value:5.49E-5||SGD DESC:Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism Gene:GIR2(YDR152W)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Highly-acidic cytoplasmic RWD domain-containing protein of unknown function; forms a complex with Rbg2p; interacts with Rbg1p and Gcn1p; associates with translating ribosomes; putative intrinsically unstructured protein Gene:GPR1(YDL035C)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis Gene:HAL9(YOL089C)|FD-Score:3.21|P-value:6.71E-4||SGD DESC:Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication Gene:HNM1(YGL077C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Choline/ethanolamine transporter; involved in the uptake of nitrogen mustard and the uptake of glycine betaine during hypersaline stress; co-regulated with phospholipid biosynthetic genes and negatively regulated by choline and myo-inositol Gene:HOF1(YMR032W)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:Bud neck-localized, SH3 domain-containing protein required for cytokinesis; is phosphorylated by Dbf2p; regulates actomyosin ring dynamics and septin localization; interacts with the formins, Bni1p and Bnr1p, and with Cyk3p, Vrp1p, and Bni5p Gene:IRS4(YKR019C)|FD-Score:3.77|P-value:8.31E-5||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KTR7(YIL085C)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR7 has a paralog, KTR5, that arose from the whole genome duplication Gene:MEC3(YLR288C)|FD-Score:-3.64|P-value:1.35E-4||SGD DESC:DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1 Gene:MPC2(YHR162W)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium Gene:OSH3(YHR073W)|FD-Score:3.13|P-value:8.74E-4||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PAM1(YDR251W)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Essential protein of unknown function; exhibits variable expression during colony morphogenesis; overexpression permits survival without protein phosphatase 2A, inhibits growth, and induces a filamentous phenotype Gene:PPA2(YMR267W)|FD-Score:3.21|P-value:6.71E-4||SGD DESC:Mitochondrial inorganic pyrophosphatase, required for mitochondrial function and possibly involved in energy generation from inorganic pyrophosphate Gene:PPM1(YDR435C)|FD-Score:3.21|P-value:6.75E-4||SGD DESC:Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits Gene:PRM2(YIL037C)|FD-Score:4.21|P-value:1.29E-5||SGD DESC:Pheromone-regulated protein, predicted to have 4 transmembrane segments and a coiled coil domain; regulated by Ste12p; required for efficient nuclear fusion Gene:PUT2(YHR037W)|FD-Score:-6.25|P-value:2.09E-10||SGD DESC:Delta-1-pyrroline-5-carboxylate dehydrogenase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of the human homolog causes HPII, an autosomal recessive inborn error of metabolism Gene:REV1(YOR346W)|FD-Score:-3.15|P-value:8.25E-4||SGD DESC:Deoxycytidyl transferase; involved in repair of abasic sites and adducted guanines in damaged DNA by translesion synthesis (TLS); forms a complex with the subunits of DNA polymerase zeta, Rev3p and Rev7p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RIM8(YGL045W)|FD-Score:5.11|P-value:1.64E-7||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:RNH201(YNL072W)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:Ribonuclease H2 catalytic subunit, removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome Gene:RPL24B(YGR148C)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication Gene:RSM24(YDR175C)|FD-Score:-3.25|P-value:5.74E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RTC2(YBR147W)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; positive regulation by Lys14p suggests that lysine may be the primary substrate; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter Gene:RTT103(YDR289C)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition Gene:SEC66(YBR171W)|FD-Score:4.79|P-value:8.18E-7||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SHE4(YOR035C)|FD-Score:-3.51|P-value:2.28E-4||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SLT2(YHR030C)|FD-Score:4.29|P-value:9.08E-6||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNF12(YNR023W)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive Gene:SNF7(YLR025W)|FD-Score:-3.92|P-value:4.46E-5||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SOV1(YMR066W)|FD-Score:10.1|P-value:2.89E-24||SGD DESC:Mitochondrial protein of unknown function Gene:SPF1(YEL031W)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SPT7(YBR081C)|FD-Score:3.77|P-value:8.22E-5||SGD DESC:Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex Gene:SSF2(YDR312W)|FD-Score:4.51|P-value:3.29E-6||SGD DESC:Protein required for ribosomal large subunit maturation; functionally redundant with Ssf1p; member of the Brix family; SSF2 has a paralog, SSF1, that arose from the whole genome duplication Gene:SUR4(YLR372W)|FD-Score:3.23|P-value:6.09E-4||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SVF1(YDR346C)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Protein with a potential role in cell survival pathways, required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance Gene:SYF2(YGR129W)|FD-Score:-4.95|P-value:3.63E-7||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:TAT1(YBR069C)|FD-Score:7.13|P-value:5.13E-13||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TFB5(YDR079C-A)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Component of the RNA polymerase II general transcription and DNA repair factor TFIIH; involved in transcription initiation and in nucleotide-excision repair; homolog of Chlamydomonas reinhardtii REX1-S protein involved in DNA repair Gene:THI12(YNL332W)|FD-Score:4.06|P-value:2.40E-5||SGD DESC:Protein involved in synthesis of the thiamine precursor hydroxymethylpyrimidine (HMP); member of a subtelomeric gene family including THI5, THI11, THI12, and THI13 Gene:UBC5(YDR059C)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived, abnormal, or excess proteins, including histone H3; central component of the cellular stress response; expression is heat inducible; protein abundance increases in response to DNA replication stress; UBC5 has a paralog, UBC4, that arose from the whole genome duplication Gene:UIP4(YPL186C)|FD-Score:5.11|P-value:1.63E-7||SGD DESC:Protein that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates; detected in a phosphorylated state in the mitochondrial outer membrane; also detected in ER and nuclear envelope Gene:URA7(YBL039C)|FD-Score:-3.34|P-value:4.24E-4||SGD DESC:Major CTP synthase isozyme (see also URA8); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA7 has a paralog, URA8, that arose from the whole genome duplication Gene:URM1(YIL008W)|FD-Score:3.86|P-value:5.69E-5||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:VAM3(YOR106W)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region Gene:VAM6(YDL077C)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p Gene:VBA2(YBR293W)|FD-Score:4.97|P-value:3.29E-7||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VMA9(YCL005W-A)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis Gene:VPS36(YLR417W)|FD-Score:3.72|P-value:9.78E-5||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:VPS61(YDR136C_d)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YBR016W(YBR016W)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Tail-anchored plasma membrane protein with a conserved CYSTM module; predicted to be palmitoylated; has similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions; YBR016W has a paralog, YDL012C, that arose from the whole genome duplication Gene:YCL012C(YCL012C)|FD-Score:3.14|P-value:8.48E-4||SGD DESC:Putative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene Gene:YCR025C(YCR025C_d)|FD-Score:10.6|P-value:9.96E-27||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR025C is not an essential gene Gene:YDR230W(YDR230W_d)|FD-Score:3.14|P-value:8.42E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20 Gene:YIL032C(YIL032C_d)|FD-Score:-3.37|P-value:3.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIR044C(YIR044C)|FD-Score:-4.83|P-value:6.99E-7||SGD DESC:Possible pseudogene in strain S288C; YIR044C and the adjacent ORF, YIR043C, together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family Gene:YKR051W(YKR051W_p)|FD-Score:3.26|P-value:5.67E-4||SGD DESC:Putative protein of unknown function Gene:YLR112W(YLR112W_d)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML131W(YML131W_p)|FD-Score:3.1|P-value:9.74E-4||SGD DESC:Protein of unknown function; similar to medium chain dehydrogenase/reductases; expression induced by stresses including osmotic shock, DNA damaging agents, and other chemicals; GFP-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YMR265C(YMR265C_p)|FD-Score:4.43|P-value:4.64E-6||SGD DESC:Putative protein of unknown function Gene:YNL108C(YNL108C_p)|FD-Score:4.28|P-value:9.34E-6||SGD DESC:Putative protein of unknown function; similar to prokaryotic phosphotransfer enzymes; null mutant shows alterations in glucose metabolism; GFP-fusion protein localizes to the cytoplasm and nucleus; YNL108C has a paralog, TFC7, that arose from the whole genome duplication Gene:YNL198C(YNL198C_d)|FD-Score:-4.11|P-value:2.00E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOX1(YML027W)|FD-Score:3.2|P-value:6.93E-4||SGD DESC:Homeobox transcriptional repressor; binds to Mcm1p and to early cell cycle boxes (ECBs) in the promoters of cell cycle-regulated genes expressed in M/G1 phase; expression is cell cycle-regulated; potential Cdc28p substrate; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOX1 has a paralog, YHP1, that arose from the whole genome duplication Gene:YPR084W(YPR084W_p)|FD-Score:3.91|P-value:4.67E-5||SGD DESC:Putative protein of unknown function

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPL235W8.034.69E-161.92RVB2ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly
YGR013W6.124.72E-101.39SNU71Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart
YLR208W4.731.14E-60.25SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YGL116W4.483.75E-60.27CDC20Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
YHR070W4.211.25E-50.34TRM5tRNA(m(1)G37)methyltransferase, methylates a tRNA base adjacent to the anticodon that has a role in prevention of frameshifting; highly conserved across Archaea, Bacteria, and Eukarya
YCR054C3.875.38E-50.34CTR86Essential protein of unknown function; with orthologs in Ashbya gossypii and Candida albicans; similar to human ATXN10, mutations in which cause spinocerebellar ataxia type 10; codon usage corresponds to that observed for yeast genes expressed at low levels; relative distribution to the nucleus increases upon DNA replication stress
YDR086C3.532.07E-40.06SSS1Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p
YJL087C3.472.57E-40.04TRL1tRNA ligase, required for tRNA splicing and for both splicing and translation of HAC1 mRNA in the UPR; has phosphodiesterase, polynucleotide kinase, and ligase activities; localized at the inner nuclear envelope and partially to polysomes
YAL038W3.442.95E-40.15CDC19Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication
YOL120C3.285.16E-40.02RPL18ARibosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication
YJR076C3.265.52E-40.01CDC11Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YER023W3.255.76E-40.03PRO3Delta 1-pyrroline-5-carboxylate reductase, catalyzes the last step in proline biosynthesis
YGL155W3.226.42E-40.01CDC43Beta subunit of geranylgeranyltransferase type I, catalyzes geranylgeranylation to the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis
YLR277C3.216.68E-40.20YSH1Putative endoribonuclease; subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs; protein abundance increases in response to DNA replication stress; YSH1 has a paralog, SYC1, that arose from the whole genome duplication
YJL097W3.010.001310.08PHS1Essential 3-hydroxyacyl-CoA dehydratase of the ER membrane, involved in elongation of very long-chain fatty acids; evolutionarily conserved, similar to mammalian PTPLA and PTPLB; involved in sphingolipid biosynthesis and protein trafficking

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YCR025C_d10.609.96E-27YCR025C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR025C is not an essential gene
YMR066W10.102.89E-24SOV1Mitochondrial protein of unknown function
YNL051W8.324.49E-17COG5Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YML071C7.291.55E-13COG8Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YBR069C7.135.13E-13TAT1Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress
YGR167W5.521.67E-8CLC1Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W
YPL186C5.111.63E-7UIP4Protein that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates; detected in a phosphorylated state in the mitochondrial outer membrane; also detected in ER and nuclear envelope
YGL045W5.111.64E-7RIM8Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family
YBR293W4.973.29E-7VBA2Permease of basic amino acids in the vacuolar membrane
YBR171W4.798.18E-7SEC66Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
YDR312W4.513.29E-6SSF2Protein required for ribosomal large subunit maturation; functionally redundant with Ssf1p; member of the Brix family; SSF2 has a paralog, SSF1, that arose from the whole genome duplication
YMR265C_p4.434.64E-6YMR265C_pPutative protein of unknown function
YJR058C4.308.41E-6APS2Small subunit of the clathrin-associated adaptor complex AP-2, which is involved in protein sorting at the plasma membrane; related to the sigma subunit of the mammalian plasma membrane clathrin-associated protein (AP-2) complex
YHR030C4.299.08E-6SLT2Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway
YNL108C_p4.289.34E-6YNL108C_pPutative protein of unknown function; similar to prokaryotic phosphotransfer enzymes; null mutant shows alterations in glucose metabolism; GFP-fusion protein localizes to the cytoplasm and nucleus; YNL108C has a paralog, TFC7, that arose from the whole genome duplication

GO enrichment analysis for SGTC_1034
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1711.30E-39SGTC_1039k821-0043 94.0 μMChemDiv (Drug-like library)159927680.102564Golgi
0.1561.65E-33SGTC_10603448-7350 82.0 μMChemDiv (Drug-like library)5701780.0483871Golgi
0.1217.76E-21SGTC_6320781-2353 4.7 μMChemDiv (Drug-like library)68115150.169014Golgi
0.1211.36E-20SGTC_33389144300 39.6 μMChembridge (Drug-like library)49012740.0921053Golgi
0.1181.27E-19SGTC_24605552655 174.2 μMMiscellaneous54019890.163934amide catabolism
0.1171.70E-19SGTC_28909046729 58.4 μMChembridge (Drug-like library)9033290.142857Golgi
0.1132.33E-18SGTC_9332914-0560 114.0 μMChemDiv (Drug-like library)4638870.12069
0.1111.09E-17SGTC_31869110464 49.5 μMChembridge (Drug-like library)170180210.0615385RPP1 & pyrimidine depletion
0.1111.36E-17SGTC_2737bleomycin 30.5 nMMiscellaneous162113180.0470588Golgi
0.1094.28E-17SGTC_15998-chlro-1-tetrahydronorharmanone 90.6 μMTimTec (Pure natural product library)41748400.078125
0.1095.14E-17SGTC_14204'-methoxyflavone 42.0 μMChemDiv (Drug-like library)777930.030303
0.1071.50E-16SGTC_1669st015249 58.4 μMTimTec (Natural product derivative library)7227930.056338Golgi
0.1071.93E-16SGTC_5571315-0110 16.2 μMChemDiv (Drug-like library)68338010.0882353Golgi
0.1055.37E-16SGTC_1025k007-0731 70.0 μMChemDiv (Drug-like library)62561180.0806452
0.1055.25E-16SGTC_14780453-0656 35.7 μMChemDiv (Drug-like library)52584960.0909091

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_772k072-017235.3 μM0.4705885861980ChemDiv (Drug-like library)338.246024.77615
SGTC_519k048-001162.8 μM0.3888896836379ChemDiv (Drug-like library)312.151341.63825
SGTC_340k038-0059454 μM0.32142923733424ChemDiv (Drug-like library)400.375086.53702ubiquinone biosynthesis & proteasome
SGTC_208k072-009812.37 μM0.2641515948901ChemDiv (Drug-like library)231.68092.96213heme biosynthesis & mitochondrial translocase
SGTC_352k048-00075.11 μM0.2586216811911ChemDiv (Drug-like library)404.484363.34223ERAD & cell cycle
SGTC_7334069-000756.1 μM0.2586215717425ChemDiv (Drug-like library)309.193764.32814ERAD & cell cycle
SGTC_242556286817.85 μM0.2456145714958Miscellaneous259.349964.02715
SGTC_3031348-166834.2 μM0.2413795380509ChemDiv (Drug-like library)292.13131.61324
SGTC_1462k081-001426.5 μM0.2372883270634ChemDiv (Drug-like library)329.5521833.08213
SGTC_2943906852945.32 μM0.2372883270634Yeast Active Library329.5521833.08213